Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure determination by cryoEM at 100 keV.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 120, Issue 49, Page e2312905120, Year 2023
Publish date	Dec 5, 2023
Authors	Greg McMullan / Katerina Naydenova / Daniel Mihaylov / Keitaro Yamashita / Mathew J Peet / Hugh Wilson / Joshua L Dickerson / Shaoxia Chen / Giuseppe Cannone / Yang Lee / Katherine A Hutchings / Olivia Gittins / Mohamed A Sobhy / Torquil Wells / Mohamed M El-Gomati / Jason Dalby / Matthias Meffert / Clemens Schulze-Briese / Richard Henderson / Christopher J Russo /
PubMed Abstract	Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose- ...Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.
External links	Proc Natl Acad Sci U S A / PubMed:38011573 / PubMed Central
Methods	EM (single particle)
Resolution	2.6 - 4.5 Å
Structure data	17958 8pv9 EMDB-17958, PDB-8pv9: Structure of DPS determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 2.7 Å 17959 8pva EMDB-17959, PDB-8pva: Structure of bacterial ribosome determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 4.5 Å 17960 8pvb EMDB-17960, PDB-8pvb: Structure of GABAAR determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.6 Å 17961 8pvc EMDB-17961, PDB-8pvc: Structure of mouse heavy-chain apoferritin determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 2.6 Å 17962 8pvd EMDB-17962, PDB-8pvd: Structure of catalase determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.4 Å 17963 8pve EMDB-17963, PDB-8pve: Structure of AHIR determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.3 Å 17964 8pvf EMDB-17964, PDB-8pvf: Structure of GAPDH determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 2.9 Å 17965 8pvg EMDB-17965, PDB-8pvg: Structure of E. coli glutamine synthetase determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.4 Å 17966 8pvh EMDB-17966, PDB-8pvh: Structure of human apo ALDH1A1 determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 2.9 Å 17967 8pvi EMDB-17967, PDB-8pvi: Structure of PaaZ determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.7 Å 17968 8pvj EMDB-17968, PDB-8pvj: Structure of lumazine synthase determined by cryoEM at 100 keV Method: EM (single particle) / Resolution: 3.0 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-R16: HEXADECANE ChemComp-D10: DECANE ChemComp-HSM: HISTAMINE / neurotransmitter, hormoneYM ChemComp-CL: Unknown entry ChemComp-V8D: Etomidate ChemComp-FE: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM
Source	escherichia coli (E. coli) homo sapiens (human) lama glama (llama) mus musculus (house mouse) thermus thermophilus (bacteria) cryptosporidium parvum (eukaryote) aquifex aeolicus (bacteria)
Keywords	DNA BINDING PROTEIN / RIBOSOME / MEMBRANE PROTEIN / Pentameric ligand-gated ion channel / Neurotrasmitter receptor / GABA(A) receptor / METAL BINDING PROTEIN / Iron storage / OXIDOREDUCTASE / GAPDH / BIOSYNTHETIC PROTEIN / Glutamine synthetase / filament / Oxidoreductase Aldehyde dehydrogenase / HYDROLASE / substrate channeling / bi-functional enzyme / dehydrogenase / TRANSFERASE / LUMAZINE SYNTHASE