EMDB-13241: E. faecalis 70S ribosome bound by PoxtA-EQ2, high-resolution comb...

Basic information

• Entry: Database: EMDB / ID: EMD-13241
• Title: E. faecalis 70S ribosome bound by PoxtA-EQ2, high-resolution combined volume
• Map data: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Half-map.

Sample

• Complex: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state I.
  • Complex: PoxA
    • Protein or peptide: x 1 types
  • Complex: 70S RibosomeRibosome
    • Protein or peptide: x 46 types
    • RNA: x 5 types
• Protein or peptide: x 1 types
• Ligand: x 7 types

Function / homology

Function:

ribosomal large subunit assembly / large ribosomal subunit / regulation of translation / small ribosomal subunit / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / zinc ion binding / cytoplasm

Domain/homology:

Ribosomal protein L31 type B / Ribosomal protein L1, bacterial-type / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 / Ribosomal protein L1 signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein S16 domain superfamily / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein S15, bacterial-type / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family / Ribosomal protein S6 / Ribosomal protein S6

Component:

Large ribosomal subunit protein bL33C / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS15 / 50S ribosomal protein L1 / Small ribosomal subunit protein bS20 / 30S ribosomal protein S2 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / 50S ribosomal protein L31 type B / Large ribosomal subunit protein bL32C / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14C / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / 50S ribosomal protein L2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / 50S ribosomal protein L13

• Biological species: Enterococcus faecalis (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.4 Å
• Authors: Crowe-McAuliffe C / Wilson DN

Funding support

Germany, Sweden, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	WI3285/8-1	Germany
Swedish Research Council	2017-03783	Sweden

Citation

Journal: mSphere / Year: 2018
Title: The Enterococcus Cassette Chromosome, a Genomic Variation Enabler in Enterococci.
Authors: A Sivertsen / J Janice / T Pedersen / T M Wagner / J Hegstad / K Hegstad /
Abstract: has a highly variable genome prone to recombination and horizontal gene transfer. Here, we have identified a novel genetic island with an insertion locus and mobilization genes similar to those of staphylococcus cassette chromosome elements SCC This novel element termed the enterococcus cassette chromosome (ECC) element was located in the 3' region of and encoded large serine recombinases similar to SCC Horizontal transfer of an ECC element termed ECC:: containing a knock-in chloramphenicol resistance determinant occurred in the presence of a conjugative plasmid. We determined the ECC:: insertion site in the 3' region of in the recipient by long-read sequencing. ECC:: also mobilized by homologous recombination through sequence identity between flanking insertion sequence (IS) elements in ECC:: and the conjugative plasmid. The genes were found in 69 of 516 genomes in GenBank. Full-length ECC elements were retrieved from 32 of these genomes. ECCs were flanked by and sites of approximately 50 bp. The sequences were found by PCR and sequencing of circularized ECCs in three strains. The genes in ECCs contained an amalgam of common and rare genes. Taken together, our data imply that ECC elements act as hot spots for genetic exchange and contribute to the large variation of accessory genes found in is a bacterium found in a great variety of environments, ranging from the clinic as a nosocomial pathogen to natural habitats such as mammalian intestines, water, and soil. They are known to exchange genetic material through horizontal gene transfer and recombination, leading to great variability of accessory genes and aiding environmental adaptation. Identifying mobile genetic elements causing sequence variation is important to understand how genetic content variation occurs. Here, a novel genetic island, the enterococcus cassette chromosome, is shown to contain a wealth of genes, which may aid in adapting to new environments. The transmission mechanism involves the only two conserved genes within ECC, , large serine recombinases that insert ECC into the host genome similarly to SCC elements found in staphylococci.

#1: Journal: Biorxiv / Year: 2021
Title: Structural basis for PoxtA-mediated resistance to Phenicol and Oxazolidinone antibiotics
Authors: Crowe-McAuliffe C / Murina V / Kasari M / Takada H / Turnbull KJ / Sundsfjord A / Hegstad K / Atkinson GC / Wilson DN / Hauryliuk V

History

Deposition	Jul 20, 2021	-
Header (metadata) release	Feb 23, 2022	-
Map release	Feb 23, 2022	-
Update	Dec 7, 2022	-
Current status	Dec 7, 2022	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_13241.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Half-map.
• Voxel size: X=Y=Z: 0.82 Å

Density

Contour Level	By AUTHOR: 0.022 / Movie #1: 0.01
Minimum - Maximum	-0.015773524 - 0.087937884
Average (Standard dev.)	0.00052956253 (±0.003905023)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 344.4 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	0.82	0.82	0.82
M x/y/z	420	420	420
origin x/y/z	0.000	0.000	0.000
length x/y/z	344.400	344.400	344.400
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	420	420	420
D min/max/mean	-0.016	0.088	0.001

Supplemental data

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_1.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_2.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Large-subunit from multibody refinement. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_3.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Small subunit body from multibody refinement. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_4.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. E-site volume from multibody refinement. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_5.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Small subunit head from multibody refinement. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_6.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Small subunit head from multibody refinement. Sharpened map.

Additional map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_additional_7.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Central protuberance volume from multibody refinement. Sharpened map.

Half map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_half_map_1.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Half-map.

Half map: PoxtA bound to 70S ribosome from E. faecalis,...

• File: emd_13241_half_map_2.map
• Annotation: PoxtA bound to 70S ribosome from E. faecalis, high-resolution combined volume. Small subunit head from multibody refinement. Sharpened map.

Sample components

Entire : Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (...

• Entire: Name: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state I.

Components

• Complex: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state I.
  • Complex: PoxA
    • Protein or peptide: ARE-ABC-F family resistance factor PoxtA
  • Complex: 70S RibosomeRibosome
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31 type BRibosome
    • Protein or peptide: 50S ribosomal protein L32-3Ribosome
    • Protein or peptide: 50S ribosomal protein L33 3Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 23S rRNA23S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • RNA: fMet-tRNA
    • Protein or peptide: 50S ribosomal protein L1
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • RNA: 16S rRNA
    • RNA: mRNAMessenger RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
• Protein or peptide: 30S ribosomal protein S11
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM IONPotassium
• Ligand: ZINC ION
• Ligand: 1,4-DIAMINOBUTANEPutrescine
• Ligand: SPECTINOMYCIN
• Ligand: water

Supramolecule #1: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (...

• Supramolecule: Name: Complex of PoxtA-EQ2 bound to the 70S ribosome from E. faecalis (initiation complex), state I.; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43, #45-#53; Details: Obtained by affinity purification of FLAG-tagged PoxtA.

Supramolecule #2: PoxA

• Supramolecule: Name: PoxA / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 / Details: ARE-ABC-F family resistance factor PoxtA
• Source (natural): Organism: Enterococcus faecalis (bacteria)

Supramolecule #3: 70S Ribosome

• Supramolecule: Name: 70S Ribosome / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#43, #45-#53 / Details: 70S ribosome
• Source (natural): Organism: Enterococcus faecalis (bacteria)

Macromolecule #1: ARE-ABC-F family resistance factor PoxtA

• Macromolecule: Name: ARE-ABC-F family resistance factor PoxtA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 67.890656 KDa
• Recombinant expression: Organism: Enterococcus faecalis (bacteria)

Sequence

String:

MRGQKMNLTF GLEEVYEDAE FHLGDFDKVG IVGVNGAGKT TLFRLLLREL KLDKGKITIG NSRIGYLPQE IIIMDEKMTV LEFLAEGRP IKKMETELNY IYEKLTVVDD DKQDRLLKRM GWLQEQLEYF DCYEAESILL DLVDSMQIDF DLFDQPISEL S GGQKSKIS FAKVLYSKPE ILLLDQPTNH LDSSTKEFVT KYLKSYRGSV LIISHDIDFL NQIIDKIMYI DKVTHKISIY EG DYYTYKK KYSENQLLRE KMIIQQEKEV KELATFVQKA KQASQTNHAL KRMGLERAIR LDKKKKNLTK RDKVYKRVKM DIK PNREVA RTPLKVENVT FHYPGHPILY KDLSFQISGK ERFLVVGENG VGKSTLLKLL MGINIPDKGK IIFNDKTDVA YYSQ ELEQL DEEKTILENV KSDEYTDWKL RATLSNFLFY DDDVNKKVNV LSPGEKARIV LCKVLLQKAN LLILDQPTNH LDPET QSII GANFNLFEGT IIVVSHNPSF VEQIGINRML ILPSGNIDNY SPELLEYYSN LNMIKEIQVG HHHHHHAKGG ENLYFQ GVA DYKDHDGDYK DHDIDYKDDD DKG

Macromolecule #2: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 7.342562 KDa

Sequence

String:

MKVKEIRELT TAEMLDKEKQ LKEELFNLRF QLATGQLENT ARIKEVRQSI ARIKTVLREQ AN

Macromolecule #3: 50S ribosomal protein L30

• Macromolecule: Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 6.365469 KDa

Sequence

String:

MAELKITLKR SVIGRPQNQR ATVKALGLGK VNSTVTKPAN EAIKGMVNTI SHLVDVEEV

Macromolecule #4: 50S ribosomal protein L31 type B

• Macromolecule: Name: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.138101 KDa

Sequence

String:

MKQDIHPNYQ PVVFMDSTTG FKFLSGSTKG SSETVEWEDG NTYPLLRVEV TSDSHPFYTG RQKFTQADGR VDRFNKKYGL KDENANPDA

Macromolecule #5: 50S ribosomal protein L32-3

• Macromolecule: Name: 50S ribosomal protein L32-3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 6.548759 KDa

Sequence

String:

MAVPARRTSK AKKAKRRTHY KLTIKGLNAC SNCGEMKKSH HVCPACGHYD GKDVMSKEA

Macromolecule #6: 50S ribosomal protein L33 3

• Macromolecule: Name: 50S ribosomal protein L33 3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 6.052027 KDa

Sequence

String:

MRVNITLECT SCKERNYLTN KNKRNNPDRL EKQKYCPRER KVTLHRETK

Macromolecule #7: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 5.373507 KDa

Sequence

String:

MKRTYQPNKR KRQKVHGFRK RMSTKNGRRV LASRRRKGRK VISA

Macromolecule #8: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 7.727316 KDa

Sequence

String:

MPKQKTHRGL AKRVKRTGGG GLKRGRAFTS HRFHGKTKKQ RRQLRKASMV AKGDYKRIRQ QLARMK

Macromolecule #9: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 4.440534 KDa

Sequence

String:

MKVRPSVKPM CEHCKVIRRK GRVMVICPAN PKHKQRQG

Macromolecule #13: 50S ribosomal protein L1

• Macromolecule: Name: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 24.49523 KDa

Sequence

String:

MAKKSKKMQE ALKKVDATKA YSVEEAVALA KDTNIAKFDA TVEVAYKLNV DPKKADQQIR GAVVLPNGTG KTQTVLVFAK GEKAKEAEA AGADFVGDDD MVAKIQGGWF DFDVVVATPD MMATVGKLGR VLGPKGLMPN PKTGTVTMDV TKAVEEVKAG K VTYRVDKA GNIHVPIGKV SFDNEKLVEN FNTINDVLLK AKPSTAKGQY IKNISVTTTF GPGIHVDQAS F

Macromolecule #14: 50S ribosomal protein L2

• Macromolecule: Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 30.161828 KDa

Sequence

String:

MAIKKYKPTT NGRRNMTSSD FAEITTSTPE KSLLQPLKNN AGRNNNGRIT VRHQGGGHKR QYRVIDFKRN KDNVAAVVKT IEYDPNRSA NIALVHYEDG VKAYILAPKG LEVGMRLVSG PEADIKVGNA LPLENIPVGT VIHNIEMKPG KGGQLIRSAG T SAQVLGKE GKYVLIRLNS GEVRMILATC RATIGSVGNE QHELINIGKA GRSRWMRKRP TVRGSVMNPN DHPHGGGEGK TP IGRKAPV SPWGQPAIGY KTRNKKAKSD KLIVRRRT

Macromolecule #15: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 22.758479 KDa

Sequence

String:

MTKGILGKKV GMTQIFTESG ELIPVTVVEA TPNVVLQVKT VETDGYEAIQ VGYQDKREVL SNKPAKGHVA KANTAPKRFI KEFKNVELG EYEVGKEIKV DVFQAGDVVD VTGTTKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV APNRVFKNKR L AGRMGGDR VTIQNLEVVK VDVERNVILI KGNIPGAKKS LITIKSAVKA K

Macromolecule #16: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 22.549717 KDa

Sequence

String:

MPNVALFKQD GTQNGEITLN EEIFGIEPNE SVVYDAIIMQ RASLRQGTHA VKNRSAVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KFPKKVRRLA MKSVLSDKVA ENNLVAVEGL SFDAPKTKEF KQVLANLSID TKVLVVLENG N DFAALSAR NLPNVSVVTS DNVSVLDVVS ANKVLATQTA LTQIEEVLA

Macromolecule #17: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 20.12349 KDa

Sequence

String:

MNRLKEKYIK EVTPSLVEKF NYSSVMQTPK VDKIVINMGV GDAVSNAKNL DKAVEELALI TGQKPLITKA KKSIAGFRLR EGMPIGAKV TLRGERMYEF LDKLVTVSLP RVRDFHGVSK KAFDGRGNYT LGIKEQLIFP EVDYDLVDKV RGMDIVIVTT A NTDEESRE LLAQLGMPFQ K

Macromolecule #18: 50S ribosomal protein L6

• Macromolecule: Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 19.437303 KDa

Sequence

String:

MSRIGNKVVV LPAGVEIKQD GNNITVKGPK GELTREFSSD IKMNIEGNEV TFIRPNDSKE MKTIHGTTRA NFNNMVVGVS EGFQKALEL IGVGYRAQVQ GNKLTLNVGY SHPVEMTAPE GVTFEVPANT QVIVKGINKE VVGELAANIR GVRPPEPYKG K GIRYVGEF VRRKEGKTGK

Macromolecule #19: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 16.325902 KDa

Sequence

String:

MRTTYMAKAG EVERKWYVVD ATDVPLGRLS AVVASVLRGK NKPTFTPHVD TGDFVIVINA DKVKLTGKKA TDKIYYRHSM YPGGLKSVT AGELRDKNSR RLIETSVKGM LPKNTLGRKQ FTKLNVYGGA EHPHAAQQPE VLDITNLI

Macromolecule #20: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 13.165275 KDa

Sequence

String:

MIQQESRLRV ADNSGAREIL TIKVLGGSGR KTANIGDVIV ATVKQATPGG VVKKGEVVKA VIVRTKSGAR RADGSYIKFD ENAAVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVL

Macromolecule #21: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 15.602868 KDa

Sequence

String:

MKLHELKPAE GSRQVRNRVG RGTSSGNGKT AGRGQKGQKA RSGGGVRLGF EGGQTPLFRR LPKRGFTNIN RKDYAVVNLD TLNRFEDGT EVTPVVLKEA GIVKNEKAGI KVLADGELTK KLTVKAAKFS KSAQEAIEAA GGSIEVI

Macromolecule #22: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 16.261168 KDa

Sequence

String:

MLVPKRVKHR REFRGKMRGE AKGGKEVAFG EWGLQATESH WITNRQIEAA RIAMTRYMKR GGKVWIKIFP HKSYTSKAIG VRMGKGKGA PEGWVSPVKR GKIMFEIAGV PEEVAREALR LASHKLPVKT KIVKREEMGG ESNEG

Macromolecule #23: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 14.503704 KDa

Sequence

String:

MSYRKLGRTS SQRKAMLRDI TTDLIINERI VTTEARAKEV RSTVEKMITL GKRGDLHARR QAATFVRNEV ASVREEDESI VVESALQKL FNDLGPRFAE RQGGYTRILK TEPRRGDAAP MVVIEFVK

Macromolecule #24: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 12.937829 KDa

Sequence

String:

MITKPDKNKT RQKRHRRVRN KISGTAECPR LNIFRSNKNI YAQVIDDVAG VTLASASALD KEISGGTKTE TAAAVGKLVA ERAAEKGIK KVVFDRGGYL YHGRVQALAE AARENGLEF

Macromolecule #25: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 13.244479 KDa

Sequence

String:

MNPLIQELTQ EQLRTDIPAF RPGDTVRVHA KVVEGTRERI QLFEGVVIKR RGAGISETYT VRKVSNGVGV ERTFPLHTPR VAQIEVVRY GKVRRAKLYY LRALHGKAAR IKEIRR

Macromolecule #26: 50S ribosomal protein L20

• Macromolecule: Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 13.674152 KDa

Sequence

String:

MARVKGGTVT RKRRKKVLKL AKGYYGSKHT LFKSAKEQVM NSYYYAFRDR RQKKRDFRKL WIARINAAAR MNGLSYSKLM HGLKLAEID INRKMLADLA VNDAAAFTAL AEQAKDALSK

Macromolecule #27: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 11.166986 KDa

Sequence

String:

MYAIIKTGGK QVKVEVGQAI YVEKLNVEAG EKVVFDEVIL VGGESTKVGA PTVAGATVEG TVEKHGKQKK VVTFQYKPKK HSHRKQGHR QPYTKVMIEA INA

Macromolecule #28: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 12.473464 KDa

Sequence

String:

MSEQITSAKA TAKTVRTSPR KARLVIDLIR GKSVADAISI LKFTPNKSAG IIEKVLMSAV ANAENNFDLD VESLVVSEAF VNEGPTMKR FRPRAKGSAS PINKRTSHIT VVVTEK

Macromolecule #29: 50S ribosomal protein L23

• Macromolecule: Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 11.089021 KDa

Sequence

String:

MELLDVIKRP VITEKSMLAM DEKKYTFEVD TRANKTLVKQ AVESAFDVKV ANVNILNVRP KFKRMGKYAG YTKKRRKAIV TLTEDSKEI QLFEAAE

Macromolecule #30: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 11.137063 KDa

Sequence

String:

MFVKKGDKVK VITGKDKNKE GVVLAAFPKQ DKVIVEGVNV VKKHQKPNQA APQGGILEVE APIHVSNVMV IDPSNGEATK VAFKEVDGK KVRVSKKTGE VLDK

Macromolecule #31: 50S ribosomal protein L27

• Macromolecule: Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.175572 KDa

Sequence

String:

MLLTMNLQLF AHKKGGGSTS NGRDSESKRL GAKSADGQTV TGGSILYRQR GTKIYPGVNV GIGGDDTLFA KVDGVVRFER KGRDKKQVS VYPVAN

Macromolecule #32: 50S ribosomal protein L28

• Macromolecule: Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 7.005314 KDa

Sequence

String:

MAKVCYFTGR KTSSGNNRSH AMNSTKRTVK PNLQKVRVLI DGKPKKVWVS TRALKSGKIE RV

Macromolecule #35: 30S ribosomal protein S2

• Macromolecule: Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 29.500635 KDa

Sequence

String:

MAVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKLV DAAYDYMKNV AEEGGVALFV GTKKQAQEAI KDEAIRAGQ YYVNHRWLGG TLTNWDTIQK RIARLKKINA MEEDGTFEVL PKKEVAGLNK ERERLEKFLG GIADMPRIPD V MYIVDPRK ERIAVQEAHK LNIPIVAMVD TNCDPDEIDV VIPSNDDAIR AVKLITAKMA DAFIEGNQGE DQATEELFVE ET PEATSIE EIVDVVEGNN ESAE

Macromolecule #36: 30S ribosomal protein S3

• Macromolecule: Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 24.415184 KDa

Sequence

String:

MGQKVHPIGM RVGIIRDWDA KWYAEKEYAE FLHEDLRIRK FIATKLADAA VSTIEIERAA NRVNISIHTA KPGMVIGKGG SEVENLRKE LNKLTGKRVH INIVEIKKPD LDAKLVGEGI ARQLENRVAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARSEGYS EGTVPLHTLR ADIDYAWEEA DTTYGKLGVK VWIYRGEILP TKKNTEKGGK

Macromolecule #37: 30S ribosomal protein S4

• Macromolecule: Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 23.273652 KDa

Sequence

String:

MSRYTGPSWK VSRRLGISLS GTGKELARRP YKPGQHGPNS RGKVSEYGMQ LTEKQKLRHM YGMNERQFRT LFIKASKIKE GKHGVNFMV LLEQRLDNVV YRLGLATTRR QARQLVNHGH ITVDGKRVDI PSYHVEVGQV IGVREKSQNI STIKEAVEAT V GRPAFVSF DTEKLEGSFT RLPERDELYP EIDEALVVEY YNQKL

Macromolecule #38: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 17.444357 KDa

Sequence

String:

MVYIDPKHLE LEDRVVAINR VTKVVKGGRR LRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAIEDAKKN LVEVPMVGST IPHEVIGVF GGGRILMKPA VEGSGVAAGG PVRAVLELAG VADITSKSLG SNTPINVVRA TVEGLKQLKR AEEVAALRGK S VEEIIG

Macromolecule #39: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 11.621188 KDa

Sequence

String:

MSQDTKYEIM YIIRPNIDEE AKTALVERFD TILKDNGAEV IESKDWEKRR LAYEMNGFRE GIYHIVNVTS PSTAGAINEF DRLAKINDD IIRHMIVKVE A

Macromolecule #40: 30S ribosomal protein S7

• Macromolecule: Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 17.864625 KDa

Sequence

String:

MPRKGPVAKR DVLPDPIYNS KLVTRLINRV MVDGKRGIAA NIIYNSFDII KESTGNDPLE VFEQAMKNVM PVLEVKARRV GGSNYQVPV EVRPERRTTL GLRWVVNYAR LRGEHTMEER LAKEIMDAAN NTGASVKKRE DTHKMADANR AFAHYRW

Macromolecule #41: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 14.936396 KDa

Sequence

String:

MVMTDPIADF LTRIRNANMV KHETLEVPAS KIKRDIAEIL KREGFIRDVE YIEDDKQGVI RVFLKYGKNE ERVITNLKRI SKPGLRAYV KADEVPKVLN GLGIAIISTS EGVITDKEAR AKNIGGEVIA YVW

Macromolecule #42: 30S ribosomal protein S9

• Macromolecule: Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 14.27148 KDa

Sequence

String:

MAQVQYSGTG RRKNAVARVR LVPGTGKITV NKKDVEEYIP HADLREVINQ PFGVTETKGA YDVIVNVNGG GYAGQSGAIR HGIARALLQ VDPDFRSALK RAGLLTRDAR MVERKKPGLK KARKASQFSK R

Macromolecule #43: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 11.731739 KDa

Sequence

String:

MAKQKIRIRL KAYEHRILDQ SADKIVETAK RTGADVSGPI PLPTERSLYT VIRATHKYKD SREQFEMRTH KRLIDIVNPT PKTVDALMK LDLPSGVNIE IKL

Macromolecule #44: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 13.740897 KDa

Sequence

String:

MAAKKVSRKR RVKKNIESGV AHIHSTFNNT IVMITDTHGN ALAWSSAGSL GFKGSKKSTP FAAQMAAEAA TKVAMEHGLK TVDVTVKGP GSGREAAIRS LQATGLEVTA IRDVTPVPH(IAS) GCRPPKRRRV

Macromolecule #45: 30S ribosomal protein S12

• Macromolecule: Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 15.309817 KDa

Sequence

String:

MPTINQLVRK PRKSKVEKSD SPALNKGYNS FKKTQTNVNS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL SNLIEVTAYI PGIGHNLQE HSVVLLRGGR VKDLPGVRYH IVRGALDTAG VNDRKQSRSK YGTKRPKA

Macromolecule #46: 30S ribosomal protein S13

• Macromolecule: Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 13.595774 KDa

Sequence

String:

MARIAGVDIP RDKRVVVSLT YIYGIGNTTA KKVLANVGVS EDVRVRDLTN EQTDAIRAEI DKLKVEGDLR REVNLNIKRL MEIGSYRGI RHRRGLPTRG QNTKNNARTR KGPTKTVAGK KK

Macromolecule #47: 30S ribosomal protein S14 type Z

• Macromolecule: Name: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 7.172593 KDa

Sequence

String:

MAKKSMIAKN KRPAKHSTQA YTRCERCGRP HSVYRKFHLC RICFRELAYK GQIPGVKKAS W

Macromolecule #48: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.668236 KDa

Sequence

String:

MAISQERKNE IIKEYARHEG DTGSPEVQIA VLTEDINQLN EHARTHKKDH HSYRGLMKKI GHRRNLLAYL RKTDIQRYRE LIQRLGLRR

Macromolecule #49: 30S ribosomal protein S16

• Macromolecule: Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.35615 KDa

Sequence

String:

MAVKIRLKRM GSKKSPFYRI VVADSRSPRD GRFIETVGTY NPLKDPAEVV LKEDLVLDWL SKGAQPSDTV RNILSKEGVM KKHHEAKNV KK

Macromolecule #50: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.3321 KDa

Sequence

String:

MTEERNQRKV YQGRVVSDKM DKTITVVVET KKNHPIYGKR MKYSKKYKAH DENNTAKVGD IVKIMETRPL SATKRFRLLE VVEEAVII

Macromolecule #51: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 9.262891 KDa

Sequence

String:

MAQQRRGGRK RRKVDYIAAN HIEYIDYKDT ELLKRFISER GKILPRRVTG TGAKNQRKLT IAIKRARIMG LLPFVSDEQ

Macromolecule #52: 30S ribosomal protein S19

• Macromolecule: Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 10.586332 KDa

Sequence

String:

MGRSLKKGPF VDDHLMKKVE AQQGAEKKKV IKTWSRRSTI FPSFVGFTIA VYDGRKHVPV YIQEDMVGHK LGEFAPTRTY RGHVADDKK TKR

Macromolecule #53: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 8.97232 KDa

Sequence

String:

MPNIESAIKR VRTSANANAK NSSQTNAMRT AIKKFEEAVA AGADNVDALY NEAVKAVDMA ATKGLIHKNK ANRDKIRLSK LAK

Macromolecule #10: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 10 / Number of copies: 1
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 943.422312 KDa

Sequence

String:

GGUUAAGUGA AUAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACUAACACCG AUAUGCUUUG GGGAGCUGU AAGUAAGCUA UGAUCCAGAG AUUUCCGAAU GGGGGAACCC AAUAUCUUUU AUAGGAUAUU ACUUUUCAGU G AAUACAUA GCUGAUUAGA GGUAGACGCA GAGAACUGAA ACAUCUUAGU ACCUGCAGGA AGAGAAAGAA AAUUCGAUUC CC UGAGUAG CGGCGAGCGA AACGGGAAGA GCCCAAACCA ACAAGCUUGC UUGUUGGGGU UGUAGGACUC CGAUAUGGUA GUC UGUUAG UAUAGUUGAA GGAUUUGGAA AAUUCCGCUA AAGAGGGUGA AAGCCCCGUA GACGAAAUGC UGACAACACC UAGG AGGAU CCUGAGUACG GCGGAACACG AGAAAUUCCG UCGGAAUCCG CGGGGACCAU CCCGCAAGGC UAAAUACUCC CUAGU GACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGU GAAAAGCACC CCGGAAGGGG AGUGAAAUAG AUCCUGAAAC CGUGUG CCU ACAACAAGUC AAAGCUCGUU AAUGAGUGAU GGCGUGCCUU UUGUAGAAUG AACCGGCGAG UUACGAUUGC AUGCGAG GU UAAGUCGAAG AGACGGAGCC GCAGCGAAAG CGAGUCUGAA UAGGGCGAAU GAGUAUGUAG UCGUAGACCC GAAACCAU G UGAUCUACCC AUGUCCAGGU UGAAGGUGCG GUAAAACGCA CUGGAGGACC GAACCCACGU ACGUUGAAAA GUGCGGGGA UGAGGUGUGG GUAGCGGAGA AAUUCCAAAC GAACUUGGAG AUAGCUGGUU CUCUCCGAAA UAGCUUUAGG GCUAGCCUCG GAAUUGAGA AUGAUGGAGG UAGAGCACUG UUUGGACUAG GGGCCCAUCU CGGGUUACCG AAUUCAGAUA AACUCCGAAU G CCAUUCAU UUAUAUCCGG GAGUCAGACU GCGAGUGAUA AGAUCCGUAG UCGAAAGGGA AACAGCCCAG ACCACCAGCU AA GGUCCCA AAAUAUAUGU UAAGUGGAAA AGGAUGUGGG GUUGCACAGA CAACUAGGAU GUUGGCUUAG AAGCAGCCAC CAU UUAAAG AGUGCGUAAU AGCUCACUAG UCGAGUGACC CUGCGCCGAA AAUGUACCGG GGCUAAACAU AUUACCGAAG CUGU GGACU ACACCAUUAG GUGUAGUGGU AGGAGAGCGU UCUAAGGGCG UUGAAGGUCG AUCGUGAGGA CGGCUGGAGC GCUUA GAAG UGAGAAUGCC GGUAUGAGUA GCGAAAGACA GGUGAGAAUC CUGUCCACCG UAUGACUAAG GUUUCCUGGG GAAGGC UCG UCCGCCCAGG GUUAGUCGGG ACCUAAGCCG AGGCCGAUAG GCGUAGGCGA UGGACAACAG GUUGAUAUUC CUGUACC AG UUGUUUUUGU UUGAGCAAUG GAGGGACGCA GUAGGCUAAG GAAUGCAUGC GAUUGGAAGU GCAUGUCCAA GCAAUGAG U CUUGAGUAGA GUUAAAUGCU UUACUCUUUA AGGACAAGUU GUGACGGGGA GCGAAAUAAU AGUAGCGAAG UUCCUGACG UCACACUGCC AAGAAAAGCU UCUAGUGAGA AAACAACUGC CCGUACCGUA AACCGACACA GGUAGUCGAG GAGAGUAUCC UAAGGUGAG CGAGCGAACU CUCGUUAAGG AACUCGGCAA AAUGACCCCG UAACUUCGGG AGAAGGGGUG CUGACUUCGG U CAGCCGCA GUGAAUAGGC CCAAGCGACU GUUUAUCAAA AACACAGGUC UCUGCAAAAU CGUAAGAUGA AGUAUAGGGG CU GACGCCU GCCCGGUGCU GGAAGGUUAA GAGGAUGGGU UAGCUUCGGC GAAGCUCAGA AUUGAAGCCC CAGUAAACGG CGG CCGUAA CUAUAA(OMC)GGU CCUAAGGUAG CGAAA(5MU)UCCU UGUCGGGUAA GUUCCGACCC GCACGAAAGG CGUAAC GAU UUGGGCACUG UCUCAACGAG AGACUCGGUG AAAUUUUAGU ACCUGUGAAG AUGCAGGUUA CCCGCGACAG GACGGAA AG ACCCCAUGGA GCUUUACUGU AGUUUGAUAU UGAGUGUUUG UACCACAUGU ACAGGAUAGG UAGGAGCCGA UGAGACCG G AACGCUAGUU UCGGAGGAGG CGCUGGUGGG AUACUACCCU UGUGUUAUGA ACCCUCUAAC CCGCACCACU AAUCGUGGU GGGAGACAGU GUCAGAUGGG CAGUUUGACU G(OMG)GGCGGUCG CCUCCUAAAA GGUAACGGAG GCGCCCAAAG GUUCCC UCA GAAUGGUUGG AAAUCAUUCG AAGAGUGUAA AGGCAGAAGG GAGCUUGACU GCGAGACCUA CAAGUCGAGC AGGGACG AA AGUCGGGCUU AGUGAUCCGG UGGUUCCGCA UGGAAGGGCC AUCGCUCAAC GGAUAAAAGC UACCCUG(2MG)GG AUAA CAGGC UUAUCUCCCC CAAGAGUCCA CAUCGACGGG GAGGUUUGGC ACCUCGAUGU CGGCUCGUCG CAUCCUGGGG CUGUA GUCG GUCCCAAGGG UUGGGCUGUU CGCCCAUUAA AGCGGCACGC GAGCUGGGUU CAGAACGUCG UGAGACAGUU CGGUCC CUA UCCGUCGCGG GCGUUGGAAA UUUGAGAGGA GCUGUCCUUA GUACGAGAGG ACCGGGAUGG ACUUACCGCU GGUGUAC CA GUUGUUCUGC CAAGGGCAUU GCUGGGUAGC UAUGUAGGGA AGGGAUAAAC GCUGAAAGCA UCUAAGUGUG AAGCCCAC C UCAAGAUGAG AUUUCCCAUU UCUUUAAGAA AGUAAGACCC CUGAGAGAUG AUCAGGUAGA UAGGUUGGAA GUGGAAGGC UAGUGAUAGU UGGAGCGGAC CAAUACUAAU CGGUCGAGGA CUUAAC

Macromolecule #11: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 37.433188 KDa

Sequence

String:

UGUGGUGGCG AUAGCGAGAA GGAUACACCU GUUCCCAUGC CGAACACAGA AGUUAAGCUU CUUAGCGCCG AUUGUAGUGA AGGGUUUCC CUUUGUGAGA GUAGGACGUC GCCACGC

Macromolecule #12: fMet-tRNA

• Macromolecule: Name: fMet-tRNA / type: rna / ID: 12 / Number of copies: 1
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 24.811795 KDa

Sequence

String:

CGCGGGGUGG AGCAGUCAGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UAGGUUCAAA UCCUGCCCCC GCAACCA

Macromolecule #33: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 33 / Number of copies: 1
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 504.27875 KDa

Sequence

String:

GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CCUAAUACAU GCAAGUCGAA CGCUUCUUUC CUCCCGAGUG CUUGCACUC AAUUGGAAAG AGGAGUGGCG GACGGGUGAG UAACACGUGG GUAACCUACC CAUCAGAGGG GGAUAACACU U GGAAACAG GUGCUAAUAC CGCAUAACAG UUUAUGCCGC AUGGCAUAAG AGUGAAAGGC GCUGGCGGGU GUCGCUGAUG GA UGGACCC GCGGUGCAUU AGCUAGUUGG UGAGGUAACG GCUCACCAAG GCCACGAUGC AUAGCCGACC UGAGAGGGUG AUC GGCCAC ACUGGGACUG AGACACGGCC CAGACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCGGCAA UGGACGAAAG UCUG ACCGA GCAACGCCGC GUGAGUGAAG AAGGUUUUCG GAUCGUAAAA CUCUGUUGUU AGAGAAGAAC AAGGACGUUA GUAAC UGAA CGUCCCCUGA CGGUAUCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAG CGU UGUCCGGAUU UAUUGGGCGU AAAGCGAGCG CAGGCGGUUU CUUAAGUCUG AUGUGAAAGC CCCCGGCUCA ACCGGGG AG GGUCAUUGGA AACUGGGAGA CUUGAGUGCA GAAGAGGAGA GUGGAAUUCC AUGUGUAGCG GUGAAAUGCG UAGAUAUA U GGAGGAACAC CAGUGGCGAA GGCGGCUCUC UGGUCUGUAA CUGACGCUGA GGCUCGAAAG CGUGGGGAGC AAACAGGAU UAGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUGGAG GGUUUCCGCC CUUCAGUGCU GCAGCAAACG CAUUAAGCA CUCCGCCUGG GGAGUACGAC CGCAAGGUUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA G CAUGUGGU UUAAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUUUGAC CACUCUAGAG AUAGAGCUUU CC CUUCGGG GACAAAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGC GCAACC CUUAUUGUUA GUUGCCAUCA UUUAGUUGGG CACUCUAGCG AGACUGCCGG UGACAAACCG GAGGAAGGUG GGGA UGACG UCAAAUCAUC AUGCCCCUUA UGACCUGGGC UACACACGUG CUACAAUGGG AAGUACAACG AGUCGCUAGA CCGCG AGGU CAUGCAAAUC UCUUAAAGCU UCUCUCAGUU CGGAUUGCAG GCUGCAACUC GCCUGCAUGA AGCCGGAAUC GCUAGU AAU CGCGGAUCAG CACGCCGCGG UGAAUACGUU CCCGGGCCUU GUACACACCG CCCGUCACAC CACGAGAGUU UGUAACA CC CGAAGUCGGU GAGGUAACCU UUUUGGAGCC AGCCGCCUAA GGUGGGAUAG AUGAUUGGGG UGAAGUCGUA ACAAGGUA G CCGUAUCGG(MA6) (MA6)GGUGCGGCU GGAUCACCUC CUCCUCC

Macromolecule #34: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 34 ; Details: Sequence unknown. Likely a mix of many different mRNAs.; Number of copies: 1
• Source (natural): Organism: Enterococcus faecalis (bacteria)
• Molecular weight: Theoretical: 6.21985 KDa

Sequence

String:

GGAGGUAAAA AAAUGACAA

Macromolecule #54: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 54 / Number of copies: 2 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Macromolecule #55: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 196 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #56: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 56 / Number of copies: 153 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #57: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 57 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #58: 1,4-DIAMINOBUTANE

• Macromolecule: Name: 1,4-DIAMINOBUTANE / type: ligand / ID: 58 / Number of copies: 3 / Formula: PUT
• Molecular weight: Theoretical: 88.151 Da

Chemical component information

ChemComp-PUT:
1,4-DIAMINOBUTANE / Putrescine

Macromolecule #59: SPECTINOMYCIN

• Macromolecule: Name: SPECTINOMYCIN / type: ligand / ID: 59 / Number of copies: 1 / Formula: SCM
• Molecular weight: Theoretical: 332.35 Da

Chemical component information

ChemComp-SCM:
SPECTINOMYCIN / antibiotic*YM / Spectinomycin

Macromolecule #60: water

• Macromolecule: Name: water / type: ligand / ID: 60 / Number of copies: 3601 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 9
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2.0 nm
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK II
• Details: Grid was prepared by four applications of elution fraction from affinity purification.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Number real images: 3639 / Average exposure time: 5.0 sec. / Average electron dose: 30.255 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 203231
• Startup model: Type of model: INSILICO MODEL / In silico model: Initial model generated with RELION.
• Initial angle assignment: Type: RANDOM ASSIGNMENT
• Final 3D classification: Number classes: 6 / Software - Name: RELION (ver. 3.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
• Final reconstruction: Number classes used: 2 / Resolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 112877