+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-13111 | ||||||||||||||||||
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タイトル | Cryo-EM structure of the 1 hpf zebrafish embryo 80S ribosome | ||||||||||||||||||
マップデータ | |||||||||||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 Peptide chain elongation / Hypusine synthesis from eIF5A-lysine / Synthesis of diphthamide-EEF2 / optokinetic behavior / regulation of mRNA cis splicing, via spliceosome / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Downregulation of ERBB4 signaling / Spry regulation of FGF signaling ...Peptide chain elongation / Hypusine synthesis from eIF5A-lysine / Synthesis of diphthamide-EEF2 / optokinetic behavior / regulation of mRNA cis splicing, via spliceosome / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Downregulation of ERBB4 signaling / Spry regulation of FGF signaling / Downregulation of ERBB2:ERBB3 signaling / L13a-mediated translational silencing of Ceruloplasmin expression / SCF-beta-TrCP mediated degradation of Emi1 / SRP-dependent cotranslational protein targeting to membrane / EGFR downregulation / SCF(Skp2)-mediated degradation of p27/p21 / NF-kB is activated and signals survival / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of TGF-beta receptor signaling / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / RMTs methylate histone arginines / AUF1 (hnRNP D0) binds and destabilizes mRNA / Asymmetric localization of PCP proteins / Degradation of AXIN / Degradation of DVL / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / TNFR1-induced NF-kappa-B signaling pathway / Hedgehog ligand biogenesis / GLI3 is processed to GLI3R by the proteasome / TNFR1-mediated ceramide production / Hedgehog 'on' state / Translesion synthesis by POLI / Termination of translesion DNA synthesis / Negative regulation of MAPK pathway / Regulation of necroptotic cell death / MAPK6/MAPK4 signaling / UCH proteinases / Josephin domain DUBs / Metalloprotease DUBs / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Negative regulation of MET activity / Assembly of the pre-replicative complex / CDK-mediated phosphorylation and removal of Cdc6 / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Ubiquitin-dependent degradation of Cyclin D / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Downregulation of ERBB2 signaling / E3 ubiquitin ligases ubiquitinate target proteins / Protein methylation / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Regulation of RUNX2 expression and activity / Regulation of PTEN localization / Regulation of PTEN stability and activity / ER Quality Control Compartment (ERQC) / Regulation of signaling by CBL / Endosomal Sorting Complex Required For Transport (ESCRT) / Protein hydroxylation / KEAP1-NFE2L2 pathway / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Regulation of pyruvate metabolism / Regulation of innate immune responses to cytosolic DNA / Deactivation of the beta-catenin transactivating complex / PINK1-PRKN Mediated Mitophagy / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of RUNX3 expression and activity / mTORC1-mediated signalling / Regulation of FZD by ubiquitination / brain segmentation / p75NTR recruits signalling complexes / Autodegradation of the E3 ubiquitin ligase COP1 / G2/M Checkpoints / Interleukin-1 signaling / nucleate erythrocyte maturation / ABC-family proteins mediated transport / Regulation of TNFR1 signaling / Antigen processing: Ubiquitination & Proteasome degradation / NOD1/2 Signaling Pathway / convergent extension involved in gastrulation / Ovarian tumor domain proteases / Ub-specific processing proteases / DNA Damage Recognition in GG-NER / Formation of Incision Complex in GG-NER / Neddylation / Oxidative Stress Induced Senescence / Oncogene Induced Senescence / Regulation of TP53 Degradation / Stabilization of p53 / The role of GTSE1 in G2/M progression after G2 checkpoint / convergent extension involved in axis elongation / Neutrophil degranulation / primitive hemopoiesis / embryonic retina morphogenesis in camera-type eye 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | Danio rerio (ゼブラフィッシュ) / Zebrafish, Brachydanio rerio | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | ||||||||||||||||||
データ登録者 | Leesch F / Lorenzo-Orts L / Grishkovskaya I / Kandolf S / Belacic K / Meinhart A / Haselbach D / Pauli A | ||||||||||||||||||
資金援助 | オーストリア, スイス, 5件
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引用 | ジャーナル: Nature / 年: 2023 タイトル: A molecular network of conserved factors keeps ribosomes dormant in the egg. 著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne ...著者: Friederike Leesch / Laura Lorenzo-Orts / Carina Pribitzer / Irina Grishkovskaya / Josef Roehsner / Anastasia Chugunova / Manuel Matzinger / Elisabeth Roitinger / Katarina Belačić / Susanne Kandolf / Tzi-Yang Lin / Karl Mechtler / Anton Meinhart / David Haselbach / Andrea Pauli / 要旨: Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron ...Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg. | ||||||||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_13111.map.gz | 364.2 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-13111-v30.xml emd-13111.xml | 96.9 KB 96.9 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_13111.png | 157 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-13111 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-13111 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_13111_validation.pdf.gz | 469.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_13111_full_validation.pdf.gz | 468.8 KB | 表示 | |
XML形式データ | emd_13111_validation.xml.gz | 7.9 KB | 表示 | |
CIF形式データ | emd_13111_validation.cif.gz | 9.1 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-13111 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-13111 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_13111.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : 80S ribosome from 1 hpf zebrafish embryos
+超分子 #1: 80S ribosome from 1 hpf zebrafish embryos
+分子 #1: 18S rRNA
+分子 #76: 28S rRNA
+分子 #77: 5S rRNA
+分子 #78: 5.8S rRNA
+分子 #2: 40S ribosomal protein SA
+分子 #3: 40S ribosomal protein S3a
+分子 #4: 40S ribosomal protein S2
+分子 #5: 40S ribosomal protein S4, X isoform
+分子 #6: 40S ribosomal protein S6
+分子 #7: 40S ribosomal protein S7
+分子 #8: 40S ribosomal protein S8
+分子 #9: 40S ribosomal protein S9
+分子 #10: 40S ribosomal protein S11
+分子 #11: 40S ribosomal protein S13
+分子 #12: Ribosomal protein S14
+分子 #13: 40S ribosomal protein S17
+分子 #14: 40S ribosomal protein S21
+分子 #15: 40S ribosomal protein S15a
+分子 #16: 40S ribosomal protein S23
+分子 #17: 40S ribosomal protein S24
+分子 #18: 40S ribosomal protein S26
+分子 #19: 40S ribosomal protein S27
+分子 #20: 40S ribosomal protein S30
+分子 #21: DNA-(apurinic or apyrimidinic site) lyase
+分子 #22: Ribosomal protein S5
+分子 #23: Ribosomal protein S10
+分子 #24: 40S ribosomal protein S15
+分子 #25: Ribosomal protein S16
+分子 #26: 40S ribosomal protein S20
+分子 #27: 40S ribosomal protein S28
+分子 #28: 40S ribosomal protein S29
+分子 #29: Guanine nucleotide-binding protein subunit beta-2-like 1
+分子 #30: 40S ribosomal protein S25
+分子 #31: 40S ribosomal protein S19
+分子 #32: 40S ribosomal protein S18
+分子 #33: Zgc:103482
+分子 #34: 60S ribosomal protein L27
+分子 #35: 60S ribosomal protein L29
+分子 #36: 60S ribosomal protein L30
+分子 #37: 60S ribosomal protein L31
+分子 #38: Ribosomal protein L32
+分子 #39: 60S ribosomal protein L35a
+分子 #40: 60S ribosomal protein L34
+分子 #41: 60S ribosomal protein L35
+分子 #42: 60S ribosomal protein L36
+分子 #43: Ribosomal protein L37
+分子 #44: 60S ribosomal protein L38
+分子 #45: 60S ribosomal protein L36a
+分子 #46: Zgc:171772
+分子 #47: 60S ribosomal protein L8
+分子 #48: Ribosomal protein L3
+分子 #49: Ribosomal protein L4
+分子 #50: Ribosomal protein L5b
+分子 #51: 60S ribosomal protein L6
+分子 #52: Ribosomal protein L7
+分子 #53: 60S ribosomal protein L7a
+分子 #54: 60S ribosomal protein L9
+分子 #55: 60S ribosomal protein L11
+分子 #56: 60S ribosomal protein L13
+分子 #57: 60S ribosomal protein L14
+分子 #58: Ribosomal protein L15
+分子 #59: 60S ribosomal protein L13a
+分子 #60: 60S ribosomal protein L17
+分子 #61: Ribosomal protein L18
+分子 #62: 60S ribosomal protein L19
+分子 #63: 60S ribosomal protein L21
+分子 #64: Ribosomal protein L22
+分子 #65: 60S ribosomal protein L23
+分子 #66: 60S ribosomal protein L24
+分子 #67: Ribosomal protein L23a
+分子 #68: ATPase H+ transporting V0 subunit e1
+分子 #69: Ribosomal protein L39
+分子 #70: 60S ribosomal protein L41
+分子 #71: 60S ribosomal protein L28
+分子 #72: 60S ribosomal protein L10
+分子 #73: 60S ribosomal protein L18a
+分子 #74: 60S ribosomal protein L40
+分子 #75: 60S ribosomal protein L27a
+分子 #79: Novel protein similar to human proliferation-associated 2G4 prote...
+分子 #80: Eukaryotic translation elongation factor 2
+分子 #81: Eukaryotic translation initiation factor 5A
+分子 #82: Dap1b
+分子 #83: ZINC ION
+分子 #84: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 構成要素:
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グリッド | モデル: Quantifoil R3.5/1 / 材質: COPPER / メッシュ: 200 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 2.0 nm / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 60 sec. / 前処理 - 雰囲気: AIR | ||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 70 % / 装置: LEICA EM GP |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均電子線量: 43.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
+画像解析
-原子モデル構築 1
精密化 | プロトコル: OTHER |
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得られたモデル | PDB-7oya: |