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- EMDB-12334: VgaL, an antibiotic resistance ABCF, in complex with 70S ribosome... -

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Basic information

Entry
Database: EMDB / ID: EMD-12334
TitleVgaL, an antibiotic resistance ABCF, in complex with 70S ribosome from Listeria monocytogenes
Map dataVgaL in complex with 70S ribosome from Listeria monocytogenes. Post-processed with automatically estimated B-factor.
Sample
  • Complex: VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA from Listeria monocytogenes
    • Protein or peptide: x 47 types
    • RNA: x 5 types
  • Ligand: x 5 types
Function / homology
Function and homology information


positive regulation of translational fidelity / ATPase-coupled transmembrane transporter activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation ...positive regulation of translational fidelity / ATPase-coupled transmembrane transporter activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / large ribosomal subunit / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / zinc ion binding / ATP binding / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L31 type B / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : ...Ribosomal protein L31 type B / Ribosomal protein S14, type Z / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / ABC transporter-like, conserved site / ABC transporters family signature. / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / ABC transporter / ABC transporter-like, ATP-binding domain / Ribosomal protein S13, bacterial-type / ATP-binding cassette, ABC transporter-type domain profile. / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein S15, bacterial-type / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S2 signature 2. / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal L32p protein family
Similarity search - Domain/homology
Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL31B / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL32B ...Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL31B / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL32B / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL36 / 30S ribosomal protein S10 / 30S ribosomal protein S11 / 30S ribosomal protein S12 / 30S ribosomal protein S13 / 30S ribosomal protein S14 type Z / 30S ribosomal protein S18 / 30S ribosomal protein S19 / 30S ribosomal protein S20 / 30S ribosomal protein S3 / 30S ribosomal protein S7 / 30S ribosomal protein S8 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL23 / 50S ribosomal protein L24 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / 30S ribosomal protein S5 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / 30S ribosomal protein S9 / 30S ribosomal protein S16 / 30S ribosomal protein S2 / 30S ribosomal protein S4 / Large ribosomal subunit protein bL21 / Lmo0919 protein / 30S ribosomal protein S6 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL16 / 30S ribosomal protein S17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL30 / 30S ribosomal protein S15
Similarity search - Component
Biological speciesListeria monocytogenes EGD-e (bacteria) / Listeria monocytogenes EGD-E (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsCrowe-McAuliffe C / Turnbull KJ / Hauryliuk V / Wilson DN
Funding support Germany, Sweden, Estonia, 6 items
OrganizationGrant numberCountry
German Research Foundation (DFG)WI3285/8-1 Germany
Swedish Research Council2017-03783 Sweden
Swedish Research Council2019-01085 Sweden
European Regional Development Fund2014-2020.4.01.15-0013 Sweden
Estonian Research CouncilPRG335 Estonia
Swedish Research Council2018-00956 Sweden
CitationJournal: Nat Commun / Year: 2021
Title: Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
Authors: Caillan Crowe-McAuliffe / Victoriia Murina / Kathryn Jane Turnbull / Marje Kasari / Merianne Mohamad / Christine Polte / Hiraku Takada / Karolis Vaitkevicius / Jörgen Johansson / Zoya ...Authors: Caillan Crowe-McAuliffe / Victoriia Murina / Kathryn Jane Turnbull / Marje Kasari / Merianne Mohamad / Christine Polte / Hiraku Takada / Karolis Vaitkevicius / Jörgen Johansson / Zoya Ignatova / Gemma C Atkinson / Alex J O'Neill / Vasili Hauryliuk / Daniel N Wilson /
Abstract: Target protection proteins confer resistance to the host organism by directly binding to the antibiotic target. One class of such proteins are the antibiotic resistance (ARE) ATP-binding cassette ...Target protection proteins confer resistance to the host organism by directly binding to the antibiotic target. One class of such proteins are the antibiotic resistance (ARE) ATP-binding cassette (ABC) proteins of the F-subtype (ARE-ABCFs), which are widely distributed throughout Gram-positive bacteria and bind the ribosome to alleviate translational inhibition from antibiotics that target the large ribosomal subunit. Here, we present single-particle cryo-EM structures of ARE-ABCF-ribosome complexes from three Gram-positive pathogens: Enterococcus faecalis LsaA, Staphylococcus haemolyticus VgaA and Listeria monocytogenes VgaL. Supported by extensive mutagenesis analysis, these structures enable a general model for antibiotic resistance mediated by these ARE-ABCFs to be proposed. In this model, ABCF binding to the antibiotic-stalled ribosome mediates antibiotic release via mechanistically diverse long-range conformational relays that converge on a few conserved ribosomal RNA nucleotides located at the peptidyltransferase center. These insights are important for the future development of antibiotics that overcome such target protection resistance mechanisms.
History
DepositionFeb 10, 2021-
Header (metadata) releaseMay 5, 2021-
Map releaseMay 5, 2021-
UpdateJun 23, 2021-
Current statusJun 23, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7nhn
  • Surface level: 0.025
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12334.png

Downloads & links

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Map

FileDownload / File: emd_12334.map.gz / Format: CCP4 / Size: 166.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationVgaL in complex with 70S ribosome from Listeria monocytogenes. Post-processed with automatically estimated B-factor.
Voxel sizeX=Y=Z: 1.041 Å
Density
Contour LevelBy AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum-0.09580157 - 0.17329556
Average (Standard dev.)0.0005648646 (±0.0057928376)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions352352352
Spacing352352352
CellA=B=C: 366.432 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0411.0411.041
M x/y/z352352352
origin x/y/z0.0000.0000.000
length x/y/z366.432366.432366.432
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS352352352
D min/max/mean-0.0960.1730.001

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Supplemental data

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Additional map: VgaL in complex with 70S ribosome from Listeria...

Fileemd_12334_additional_1.map
AnnotationVgaL in complex with 70S ribosome from Listeria monocytogenes. Output of RELION Refine3D.
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram (log scale)

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Additional map: VgaL in complex with 70S ribosome from Listeria...

Fileemd_12334_additional_2.map
AnnotationVgaL in complex with 70S ribosome from Listeria monocytogenes. Filtered by local resolution.
Projections & Slices
AxesZYX

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Additional map: Multibody refinement of large ribosomal subunit and vgaL NBDs.

Fileemd_12334_additional_3.map
AnnotationMultibody refinement of large ribosomal subunit and vgaL NBDs.
Projections & Slices
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Additional map: Multibody refinement of small ribosomal subunit head.

Fileemd_12334_additional_4.map
AnnotationMultibody refinement of small ribosomal subunit head.
Projections & Slices
AxesZYX

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Additional map: Multibody refinement of small ribosomal subunit body.

Fileemd_12334_additional_5.map
AnnotationMultibody refinement of small ribosomal subunit body.
Projections & Slices
AxesZYX

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Histogram

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Half map: VgaL in complex with 70S ribosome from Listeria...

Fileemd_12334_half_map_1.map
AnnotationVgaL in complex with 70S ribosome from Listeria monocytogenes. Half map 1.
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

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Half map: VgaL in complex with 70S ribosome from Listeria...

Fileemd_12334_half_map_2.map
AnnotationVgaL in complex with 70S ribosome from Listeria monocytogenes. Half map 2.
Projections & Slices
AxesZYX

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Sample components

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Entire : VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA fro...

EntireName: VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA from Listeria monocytogenes
Components
  • Complex: VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA from Listeria monocytogenes
    • Protein or peptide: Lmo0919 protein
    • RNA: tRNA-fMet
    • RNA: RNA (5'-R(P*GP*AP*GP*GP*UP*NP*NP*NP*NP*NP*NP*AP*UP*G)-3')
    • RNA: 23S rRNA23S ribosomal RNA
    • RNA: 16S rRNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32-2Ribosome
    • Protein or peptide: 50S ribosomal protein L33 1Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 5S rRNA5S ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 50S ribosomal protein L31 type BRibosome
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: SPERMIDINE
  • Ligand: POTASSIUM IONPotassium
  • Ligand: ZINC ION

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Supramolecule #1: VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA fro...

SupramoleculeName: VgaL in complex with 70S ribosome, mRNA, and distorted P-tRNA from Listeria monocytogenes
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Recombinant expressionOrganism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 2.2 MDa

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Macromolecule #1: Lmo0919 protein

MacromoleculeName: Lmo0919 protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 64.280094 KDa
Recombinant expressionOrganism: Listeria monocytogenes EGD-e (bacteria)
SequenceString: MSTIEINQLK IEVADRVLVE IPHLLVSKKA RIGIIGQNGL GKTTLMEVIA GAKEATSGTV TTQGKLAYIK QLSTDTSTKS GGEKTRKAT QHAMRQNPSV LLADQPTSNL DVESVKHLER QWSDFHGALI IISHDRAFLD ALCTEIWEIK NQKIHVYKGN Y HAYLEQKQ ...String:
MSTIEINQLK IEVADRVLVE IPHLLVSKKA RIGIIGQNGL GKTTLMEVIA GAKEATSGTV TTQGKLAYIK QLSTDTSTKS GGEKTRKAT QHAMRQNPSV LLADQPTSNL DVESVKHLER QWSDFHGALI IISHDRAFLD ALCTEIWEIK NQKIHVYKGN Y HAYLEQKQ QQENQAELAY KEFKNKKKQL QASQTHHEIE AGRIVKPGKR LNNKEASAFK AGKGTQQKKQ HSTIKALEKR IE RLGNVEK PHTTKPIKII TPDNRVIKKG NTILSAKETA YEIAGRKLFE TKAFSIKAGD KVALIGENAS GKTTFLKEII QEN PNLLCN PQAKIAYFDQ ELNGLNQTKS LLENISEISV QTKQVNREVL GSMHFKESDL HKEVRMLSGG ERVKLLLSML LLSD ANFLI LDQPTNYLDI YAMEALETLI KQFAGTVLFV SHDRTFVNHV AEQLLVIENN EMNFHRMTFA EYEESKAPSR ITEED KLIL EMRMSEIAAK LMQPNLKPTE KAILEQDYQE IITKRQQFSG GHHHHHHAKG GENLYFQGVA DYKDHDGDYK DHDIDY KDD DDKG

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Macromolecule #6: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 30.568367 KDa
SequenceString: MAIKKYKPTT NGRRHMTSSD FAEITTSTPE KSLLRPLKKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGIPGRVAT IEYDPNRSA NIALINYADG EKRYIIAAKG LEVGQTIYSG AEADIKVGNA LELKDIPVGT VIHNIEMKPG KGGQLVRSAG T SAQVLGKE ...String:
MAIKKYKPTT NGRRHMTSSD FAEITTSTPE KSLLRPLKKK AGRNNQGKLT VRHHGGGHKR QYRVIDFKRN KDGIPGRVAT IEYDPNRSA NIALINYADG EKRYIIAAKG LEVGQTIYSG AEADIKVGNA LELKDIPVGT VIHNIEMKPG KGGQLVRSAG T SAQVLGKE GKYVLIRLNS GEVRMILATC RATIGQVGNE QHELINIGKA GRSRWMGKRP TVRGSVMNPN DHPHGGGEGK AP IGRKSPM SPWGKPTLGY KTRKKNNNSD KFIVRRRKKK

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Macromolecule #7: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 22.863459 KDa
SequenceString: MTKGILGRKV GMTQVFTENG ELIPVTVIEA AQNVVLQKKT VETDGYEAVQ IGFEDKRAIL SNKPEQGHVA KANTTPKRFI REFRDVNLD EYEIGAEVKV DVFAEGDIID ATGVSKGKGF QGVIKRHGQS RGPMAHGSRY HRRPGSMGPV APNRVFKNKL L PGRMGGEQ ...String:
MTKGILGRKV GMTQVFTENG ELIPVTVIEA AQNVVLQKKT VETDGYEAVQ IGFEDKRAIL SNKPEQGHVA KANTTPKRFI REFRDVNLD EYEIGAEVKV DVFAEGDIID ATGVSKGKGF QGVIKRHGQS RGPMAHGSRY HRRPGSMGPV APNRVFKNKL L PGRMGGEQ ITIQNLEIVK VDVEKNVLLV KGNVPGAKKA LVQIKTATKA K

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Macromolecule #8: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 22.640262 KDa
SequenceString: MPKLSLLKQD GTNAGEITLN DTVFGIEPNE KVVVDVILSQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYAY KLPKKVRRLA IKSILSSKVN EEKLVVLEGL TFDAPKTKEF AAFLKNISVD TKALIVVAGE S ENVELSAR ...String:
MPKLSLLKQD GTNAGEITLN DTVFGIEPNE KVVVDVILSQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYAY KLPKKVRRLA IKSILSSKVN EEKLVVLEGL TFDAPKTKEF AAFLKNISVD TKALIVVAGE S ENVELSAR NLQGITVIPA ESISVLEVAK HDKLIITKAA VEKVEEVLA

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Macromolecule #9: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 20.023248 KDa
SequenceString:
MNRLKDQYLK EIVPALMSKF NYDSVMEVPK IDKIVINTGV GDATANAKVL DSAVEELALI TGQKPVITKA KNSIAGFRLR EGMPIGAKV TLRGERMYDF LDKLVTVSLP RVRDFRGVSK KAFDGRGNYT LGVREQLIFP EIDYDQVSKV RGMDVVIVTT A KSDEESHE LLTQLGMPFQ K

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Macromolecule #10: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 19.434365 KDa
SequenceString:
MSRIGKKTIV IPAGVTVTLN GSTATVKGPK GELVKEFNPE ITIKIEGNEI NVSRPTDNKN HRALHGTTRA ILNNMVVGVS EGYEKKLEL IGVGYRAQKQ GDKLVLNVGY SHPVEFVAPK GVDIEVPANT QVIVKGYNKE HVGELAANIR AVRPPEPYKG K GIRYEGEH VRRKEGKTGK

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Macromolecule #11: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 16.227671 KDa
SequenceString:
MRTTYMAKPG EVERKWYVID ATGVSLGRLS SEVASILRGK NKPQFTPHID TGDFVIIINA GKIGLTGKKA TDKIYYRHSQ YPGGLKSRT AGEMRTNNPE KLLELSIKGM LPKNSLGRQL FKKLHVYGGS EHEHAAQQPE VYELRG

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Macromolecule #12: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.248427 KDa
SequenceString:
MIQQESRMKV ADNSGAREVL TIKVLGGSGR KTANIGDVVV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RQDGSYIKFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVL

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Macromolecule #13: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 15.812118 KDa
SequenceString:
MKLHELKPSE GSRKERNRVG RGTGSGNGKT SGRGHKGQKA RSGGGVRLGF EGGQLPLFRR IPKRGFTNIN RKEFAIVNLD VLNRFEDGT EVTPELLVET GIIRNEKSGI KILSNGNIEK KLTVKANKFS AAAKEAIEAA GGKTEVI

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Macromolecule #14: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 16.171043 KDa
SequenceString:
MLVPKRVKYR REFRGNMRGR AKGGTEVAFG EYGLQAVEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP HKSYTSKPIG VRMGKGKGA PEGWVSPVKR GKIMFEIAGV PEDVAREALR LAAHKLPVKT KIVKREEIGG EANES

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Macromolecule #15: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 15.246793 KDa
SequenceString:
MGYRKLGRTS SQRKALLRDL ATDLIVFERI ETTEARAKEI RKVVEKLITS GKKGDLHARR QAAAFIRHEV VEVVQVDAKG KDGSTVKKN RPVYALQKLF DDVAPRYAER QGGYTRILKK GPRRGDGAPM VIIELV

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Macromolecule #16: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.121936 KDa
SequenceString:
MITKIDKNKV RKKRHARVRS KISGTESRPR LNVFRSNKNI YAQIIDDVNG VTLASASNLD KDFGSAESKV DAASKVGELV AKRASEKGI TSVTFDRGGY LYHGRVKALA EAARENGLEF

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Macromolecule #17: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.146463 KDa
SequenceString:
MNKLIDEITK SQLNPDVPNF RPGDTVRVHA KVVEGTRERI QLFEGVVIKR RGAGISETFT VRKISNSVGV ERTFPVHTPR IAKLEVIRR GKVRRAKLYY LRNLRGKAAR IKEIR

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Macromolecule #18: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.71935 KDa
SequenceString:
MPRVKGGTVT RKRRKKIVKL AKGYYGSKHL LFKVANQAVM KSYQYAYRDR RQKKRDFRRL WIARINAAAR MQDLSYSKLM HGLKLAGID INRKMLADLA VNDIASFNTL ADSAKKALAK

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Macromolecule #19: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 11.234029 KDa
SequenceString:
MYAIIETGGK QIKVEAGQEI YVEKLAGEVG DVVTFDKVLF VGGDSAKVGV PFVEGATVTA KVEKQGRAKK LTVYKYKPKK NYHKKQGHR QPYTKLTIDA INA

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Macromolecule #20: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 12.895945 KDa
SequenceString:
MASEVTSAKA VAKTVRIAPR KARIVIDLIR GKQVGEAIAI LKYTPRSASP IIEKVLKSAI ANAEHNYDLD INNLVVEEAF VDEGPTLKR FRPRAQGRAS AINKRTSHIT VVVSEVKEG

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Macromolecule #21: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.935654 KDa
SequenceString:
MDARDIIKRP VVTEESTSIL DDKKYTFEVD TRATKTQVKY AVEEIFDVKV AKVNVMNYKG KLKRMGRYAG YTNKRRKAIV TVTADSKEI QFFEV

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Macromolecule #22: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 11.205304 KDa
SequenceString:
MHVKKGDKVK VITGKDKGKS GKVLAAFPKK DRVLIEGINM VKKHTKPSNV NPQGGILNVE APIHVSNVML IDPKTGEPTR VGYEVKGDK KVRVAKKSGE VIDK

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Macromolecule #23: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.57193 KDa
SequenceString:
MLKFDIQHFA HKKGGGSTSN GRDSESKRLG AKRADGQFVT GGSILYRQRG TKIYPGTNVG RGGDDTLFAK TDGVVRFERM GRDKKKVSV YPEVQEA

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Macromolecule #24: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 7.010383 KDa
SequenceString:
MAKECVITGR KSRSGNKRSH AMNSSKRTWK ANLQKVRILV NGKPKKVWVS ARALKSGKVE RV

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Macromolecule #25: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 7.414548 KDa
SequenceString:
MKANDIRDLS TTEIQDQEKA LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTIVRER ELA

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Macromolecule #26: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 6.504753 KDa
SequenceString:
MAKLEITLKR SLIGRPQPQR KTVQALGLGK TNSVVVKEDN PAIRGMITKV SHLVDVKEV

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Macromolecule #27: 50S ribosomal protein L32-2

MacromoleculeName: 50S ribosomal protein L32-2 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 6.534564 KDa
SequenceString:
MAVPFRRTSK AKKRKRRTHV KLQLPGMNEC SNCGEYRLSH HVCPECGQYD GKDVANS

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Macromolecule #28: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 6.021034 KDa
SequenceString:
MRVNITLECT ECGDRNYITT KNKRENPERI ELKKYCPRLR RVTLHRETK

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Macromolecule #29: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 5.319457 KDa
SequenceString:
MKRTYQPSKR KRKKVHGFRT RMSTKNGRRV LASRRRKGRK VLSA

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Macromolecule #30: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 7.746438 KDa
SequenceString:
MPKMKTHRGS AKRFKRTGSG KLKRRHGFTS HMFANKSQKQ KRKLRKSAMV SAGDFKRIRQ MVAKMK

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Macromolecule #31: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 4.33646 KDa
SequenceString:
MKVRPSVKPM CEKCKVIRRK GKVMVICENP KHKQKQG

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Macromolecule #33: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 28.417777 KDa
SequenceString: MPVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAFNFMREV ASDNGTILFV GTKKQAQESV RDEAIRSGQ YFVNHRWLGG TLTNFETIQK RIQHLKKIER MEADGTFEVL PKKEVVLLKK EQEKLERFLG GIKDMKGLPD A LFIVDPRK ...String:
MPVISMKQLL EAGVHFGHQT RRWNPKMKKY IFTERNGIYI IDLQKTVKKV DEAFNFMREV ASDNGTILFV GTKKQAQESV RDEAIRSGQ YFVNHRWLGG TLTNFETIQK RIQHLKKIER MEADGTFEVL PKKEVVLLKK EQEKLERFLG GIKDMKGLPD A LFIVDPRK ERIAVAEARK LHIPIIGIVD TNCDPDEIDY VIPANDDAIR AVKLLTAKMA DAIIEVNQGE ELTEAEVAPV EE KATEETT EA

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Macromolecule #34: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 24.587158 KDa
SequenceString: MGQKVHPIGM RIGVIRDWDS KWYAEKDYAD FLHEDLRIRD YVAKRLSDAS VSRVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKN LNELTQKRVH INIVEIKRAD LDAKLVAENI ARQLEGRVSF RRAQKQAIQR TMRAGAKGIK TQVSGRLGGA D IARAEHYS ...String:
MGQKVHPIGM RIGVIRDWDS KWYAEKDYAD FLHEDLRIRD YVAKRLSDAS VSRVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKN LNELTQKRVH INIVEIKRAD LDAKLVAENI ARQLEGRVSF RRAQKQAIQR TMRAGAKGIK TQVSGRLGGA D IARAEHYS EGTVPLHTLR ADIDYAWEEA DTTYGKLGVK VWIYRGEVLP TKKNNVEGGK

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Macromolecule #35: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 22.773992 KDa
SequenceString: MARYTGPSWK VSRRLGISLS GTGKELERRP YAPGQHGPTQ RKKISEYGLQ QAEKQKLRHM YGLTERQFKN TFNKAGKLRG KHGENFMIL LEQRLDNIVY RLGLARTRRA ARQLVNHGHI TVDGKRVDIP SYQVSVGQVI SVREKSAKNS AIAESLEVSS F VPEYVTFD ...String:
MARYTGPSWK VSRRLGISLS GTGKELERRP YAPGQHGPTQ RKKISEYGLQ QAEKQKLRHM YGLTERQFKN TFNKAGKLRG KHGENFMIL LEQRLDNIVY RLGLARTRRA ARQLVNHGHI TVDGKRVDIP SYQVSVGQVI SVREKSAKNS AIAESLEVSS F VPEYVTFD AEKLTGSLNR LPERSELAAE INEAFIVEFY SR

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Macromolecule #36: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 17.482166 KDa
SequenceString:
MPEQIDGNKL DLEERVVTIN RVAKVVKGGR RFRFTALVVV GDKNGHVGFG TGKAQEVPDA IRKAVEDAKK NMVLVPTVDT TIPHTVVGH FGGGEILLKP ASAGSGVTAG GPVRAVLELA GVADVSSKSL GSNTPINMVR ATIDGIKQLK NAEDVAKLRG K TVEELLG

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Macromolecule #37: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 11.52609 KDa
SequenceString:
MARKYEIMYI IRPNIEEDEK KAVVERFDGI LTENGAEIIE SKEWGKRRLA YEINDYRDGF YHIVKLNADK ADSINEFDRL AKISDDIIR HMVIKEEA

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Macromolecule #38: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 17.848816 KDa
SequenceString:
MPRKGPVAKR DVLPDPIYNS KLVTRLINKM MVDGKRGKSQ AILYSAFDII AQETGKDPME VFEQAMKNIM PLLEVKARRV GGANYQVPI EVRADRRSTL GLRWLVNYAR LRGEKTMEVR VAREIMDAAN NTGASVKKRE DTHKMADANR AFAHYRW

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Macromolecule #39: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 14.667118 KDa
SequenceString:
MVMTDPIADF LTRIRNANMV KHDKLELPAS KIKKEIAEIL KREGFIRDVE YIEDDNAGTI RVFLKYGATG ERVITGLKRI SKPGLRVYA KSTEVPKVLN GLGIAIVSTS QGVLTDKEAR AKQVGGEVLA YVW

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Macromolecule #40: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 14.397641 KDa
SequenceString:
MAQVQYYGTG RRKSSVARVR LVPGDGKIVI NNRDWEDYIP FAALREVIKQ PLVATETLGN YDVLVNVHGG GYTGQAGAIR HGVARALLQ VAPEYRPALK SAGLLTRDSR MKERKKPGLK GARRAPQFSK R

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Macromolecule #41: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 11.703661 KDa
SequenceString:
MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSIYT VLRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDSLMR LDLPSGVDIE IKL

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Macromolecule #42: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.812904 KDa
SequenceString:
MARKTNTRKR RVKKNIESGI AHIRSTFNNT IVMITDTHGN ALAWSSAGSL GFKGSRKSTP FAAQMAAESA AKSAQEHGLK TLEVTVKGP GSGREAAIRA LQAAGLEVTA IKDVTPVPHN GCRPPKRRRV

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Macromolecule #43: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 15.216778 KDa
SequenceString:
MPTINQLVRK PRQSKIKKST SPALNKGLNS FKRELTDVNS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL SNGIEVTAYI PGIGHNLQE HSVVLIRGGR VKDLPGVRYH IVRGALDTAG VENRGQSRSK YGTKKPKK

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Macromolecule #44: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 13.738979 KDa
SequenceString:
MARIAGVDVP REKRIVISLT YIYGIGKQTA KEVLAEAGVS EDTRTRDLTE EELGKIREIL DRIKVEGDLR REVNLNIKRL IEIGSYRGM RHRRGLPVRG QNTKNNARTR KGPSKTVAGK KK

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Macromolecule #45: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 7.164716 KDa
SequenceString:
MAKKSMIAKQ KRTPKYAVQA YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS W

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Macromolecule #46: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.656375 KDa
SequenceString:
MALTQERKNE IIAEYRVHDT DTGSPEVQIA VLTAEINSLN EHVRVHKKDH HSYRGLMKMV GHRRNLLTYL RKKDVQRYRE LIKRLGLRR

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Macromolecule #47: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.386158 KDa
SequenceString:
MAVKIRLKRI GSKKKPFYRI VVADSRFPRD GRSIETIGTY NPLLDPVEVK IDEEATLKWM HNGAKPSDTV RNLLSREGIM EKFHNQKLG K

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Macromolecule #48: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.057699 KDa
SequenceString:
MADRNQRKVY TGRVVSDKMD KTITVVVETY KKHGLYGKRV KYSKKFKAHD ENNIAKTGDV VRISETRPLS ATKHFRLLEV VEEAVII

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Macromolecule #49: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 9.119831 KDa
SequenceString:
MAGGRRGGRR RKKVCYFTSN GITHIDYKDV ELLKKFVSER GKILPRRVTG TSAKYQRKLT VAIKRSRQMA LLPFVAEEK

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Macromolecule #50: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 10.498116 KDa
SequenceString:
MGRSLKKGPF VDDHLMKKVE AAAESEKKQV IKTWSRRSTI FPTFVGQTIA VYDGRKHVPV YVQEDMVGHK LGEFAPTRTY RGHAGDDKK TKR

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Macromolecule #51: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 9.189573 KDa
SequenceString:
MPNIKSAIKR VKTAETRNSR NASQRSAMRT AIKKFDEAAA NNADNAKDLY VEASKKLDSA VSKGLIHKNN AARNKSRLAA KLAK

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Macromolecule #52: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 9.27436 KDa
SequenceString:
MKTGIHPEYR PVVFVDTSTD FKFLSGSTKS SSETIKWEDG NEYPLLRVEI SSDSHPFYTG KQKHATADGR VDRFNKKYGL K

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Macromolecule #2: tRNA-fMet

MacromoleculeName: tRNA-fMet / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 24.788754 KDa
SequenceString:
GCGGGGUGGA GCAGUCUGGU AGCUCGUCGG GCUCAUAACC CGAAGGUCGU AGGUUCAAAU CCUGCCCCCG CAACCCA

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Macromolecule #3: RNA (5'-R(P*GP*AP*GP*GP*UP*NP*NP*NP*NP*NP*NP*AP*UP*G)-3')

MacromoleculeName: RNA (5'-R(P*GP*AP*GP*GP*UP*NP*NP*NP*NP*NP*NP*AP*UP*G)-3')
type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 3.783178 KDa
SequenceString:
GAGGU(N)(N)(N)(N)(N) (N)AUG

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Macromolecule #4: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 949.689812 KDa
SequenceString: UUAAGUUAGA AAGGGCGCAC GGUGGAUGCC UUGGCACUAG GAGCCGAAGA AGGACGGGAC UAACACCGAU AUGCUUUGGG GAGCUGUAC GUAAGCGUUG AUCCAGAGAU UUCCGAAUGG GGGAACCCAC UAUCUUUAGU CGGAUAGUAU CCUUACGUGA A UACAUAGC ...String:
UUAAGUUAGA AAGGGCGCAC GGUGGAUGCC UUGGCACUAG GAGCCGAAGA AGGACGGGAC UAACACCGAU AUGCUUUGGG GAGCUGUAC GUAAGCGUUG AUCCAGAGAU UUCCGAAUGG GGGAACCCAC UAUCUUUAGU CGGAUAGUAU CCUUACGUGA A UACAUAGC GUGAGGAAGG CAGACCCAGG GAACUGAAAC AUCUAAGUAC CUGGAGGAAG AGAAAGAAAA AUCGAUUUCC UG AGUAGCG GCGAGCGAAA CGGAAAGAGC CCAAACCAAG AAGCUUGCUU CUUGGGGUUG UAGGACACUC UAUACGGAGU UAC AAAAGA AAGUUAUAAA UGAAGCGGUC UGGAAAGGCC CGCCAAAGAC GGUAACAGCC CGGUAGUUGA AAUGGCUUUC CCUC CAGAG UGGAUCCUGA GUACGGCGGA ACACGUGAAA UUCCGUCGGA AUCCGGGAGG ACCAUCUCCC AAGGCUAAAU ACUCC CUAG UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGUGAAAA GCACCCCGGA AGGGGAGUGA AACAGUUCCU GAAACC GUG UGCCUACAAG UAGUUAGAGC CCGUUAAUGG GUGAUAGCGU GCCUUUUGUA GAAUGAACCG GCGAGUUACG AUUUGUU GC AAGGUUAAGC GGAAAAAGCG GAGCCGUAGC GAAAGCGAGU CUGAAUAGGG CGCAUAAGUA ACAGGUCGUA GACCCGAA A CCAGGUGAUC UACCCAUGUC CAGGAUGAAG GUAAGGUAAU ACUUACUGGA GGUCCGAACC CACGCACGUU GAAAAGUGC GGGGAUGAGG UGUGGGUAGC GGAGAAAUUC CAAUCGAACU UGGAGAUAGC UGGUUCUCUC CGAAAUAGCU UUAGGGCUAG CCUCGAGGU AAAGAGUCAU GGAGGUAGAG CACUGUUUGG ACUAGGGGCC CUUCUCGGGU UACCGAAUUC AGAUAAACUC C GAAUGCCA UGUACUUAUA CUCGGGAGUC AGACUGCGAG UGAUAAGAUC CGUAGUCGAA AGGGAAACAG CCCAGACCAC CA GUUAAGG UCCCCAAAUA UAUGUUAAGU GGAAAAGGAU GUGGGGUUGC UUAGACAACC AGGAUGUUGG CUUAGAAGCA GCC ACCAUU GAAAGAGUGC GUAAUAGCUC ACUGGUCGAG UGACCCCGCG CCGAAAAUGU ACCGGGGCUA AACAUAUUAC CGAA ACUGU GGAUGAACCU CUUUAGAGGU UCGUGGUAGG AGAGCGUUCU AAGGGCGGUG AAGUCAGACC GGAAGGACUG GUGGA GCGC UUAGAAGUGA GAAUGCCGGU AUGAGUAGCG AAAGAAGGGU GAGAAUCCCU UCCACCGAAU AUCUAAGGUU UCCUGA GGA AGGCUCGUCC GCUCAGGGUU AGUCGGGACC UAAGCCGAGG CCGAUAGGCG UAGGCGAUGG ACAACAGGUA GAGAUUC CU GUACCAGUGC UAAUUGUUUA ACCGAUGGGG UGACACAGAA GGAUAGGGAA UCGCACGAAU GGAAAUGUGC GUCCAAGC A GUGAGUGUGA GAAGUAGGCA AAUCCGCUUC UCGCGAAGCA UGAGCUGUGA UGGGGAAGGA AAUUAAGUAC GGAAGUUCC UGAUUUCACG CUGUCAAGAA AAGCCUCUAG GAAGAGUAGU ACUGCCCGUA CCGCAAACCG ACACAGGUAG AUGAGGAGAG AAUCCUAAG GUGAGCGAGA GAACUCUCGU UAAGGAACUC GGCAAAAUGA CCCCGUAACU UCGGGAGAAG GGGUGCUCUA U UAGGGUGC AAGCCCGAGA GAGCCGCAGU GAAUAGGCCC AGGCGACUGU UUAGCAAAAA CACAGGUCUC UGCAAAACCG UA AGGUGAC GUAUAGGGGC UGACGCCUGC CCGGUGCUGG AAGGUUAAGA GGAGUGCUUA GCUUCGGCGA AGGUACGAAU UGA AGCCCC AGUAAACGGC GGCCGUAACU AUAACGGUCC UAAGGUAGCG AAAUUCCUUG UCGGGUAAGU UCCGACCCGC ACGA AAGGC GCAACGAUCU GGGCACUGUC UCAACGAGAG ACUCGGUGAA AUUAUAGUAC CUGUGAAGAU GCAGGUUACC CGCGA CAGG ACGGAAAGAC CCCGUGGAGC UUUACUGCAA CCUGAUAUGG AAUGUUUGUA CCGCUUGUAC AGGAUAGGUA GGAGCC GAA GAGACGUGUG CGCUAGCAUA CGAGGAGGCA AUGGUGGGAU ACUACCCUGG CUGUAUGACC AUUCUAACCC GCCACGC UU AGCGCGUGGG GAGACAGUGU CAGGUGGGCA GUUUGACUGG GGCGGUCGCC UCCUAAAGAG UAACGGAGGC GCCCAAAG G UUCCCUCAGA AUGGAUGGAA AUCAUUCGCA GAGUGUAAAG GCACAAGGGA GCUUGACUGC GAGACUGACA AGUCGAGCA GGGACGAAAG UCGGGCUUAG UGAUCCGGUG GUUCCGCAUG GAAGGGCCAU CGCUCAACGG AUAAAAGCUA CCCCGGGGAU AACAGGCUU AUCUCCCCCA AGAGUCCACA UCGACGGGGA GGUUUGGCAC CUCGAUGUCG GCUCGUCGCA UCCUGGGGCU G UAGUCGGU CCCAAGGGUU GGGCUGUUCG CCCAUUAAAG CGGCACGCGA GCUGGGUUCA GAACGUCGUG AGACAGUUCG GU CCCUAUC CGUCGCGGGC GCAGGAAAUU UGAGAGGAGC UGUCCUUAGU ACGAGAGGAC CGGGAUGGAC ACACCGCUGG UGU ACCAGU UGUUCCGCCA GGAGCAUCGC UGGGUAGCUA UGUGUGGCAG GGAUAAACGC UGAAAGCAUC UAAGCGUGAA GCCC CCCUC AAGAUGAGAU UUCCCAUUUC UUCGGAAAGU AAGAUCCCUG AAAGAUGAUC AGGUAGAUAG GUUUGGAGUG GAAGU GUAG CGAUACAUGG AGCGGACAAA UACUAAUCGA UCGAGGACUU AACC

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Macromolecule #5: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Listeria monocytogenes EGD-e (bacteria)
Molecular weightTheoretical: 499.378531 KDa
SequenceString: GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CCUAAUACAU GCAAGUCGAA CGAACGGAGG AAGAGCUUGC UCUUCCAAU GUUAGUGGCG GACGGGUGAG UAACACGUGG GCAACCUGCC UGUAAGUUGG GGAUAACUCC GGGAAACCGG G GCUAAUAC ...String:
GAGAGUUUGA UCCUGGCUCA GGACGAACGC UGGCGGCGUG CCUAAUACAU GCAAGUCGAA CGAACGGAGG AAGAGCUUGC UCUUCCAAU GUUAGUGGCG GACGGGUGAG UAACACGUGG GCAACCUGCC UGUAAGUUGG GGAUAACUCC GGGAAACCGG G GCUAAUAC CGAAUGAUAA AGUGUGGCGC AUGCCACGCU UUUGAAAGAU GGUUUCGGCU AUCGCUUACA GAUGGGCCCG CG GUGCAUU AGCUAGUUGG UAGGGUAAUG GCCUACCAAG GCAACGAUGC AUAGCCGACC UGAGAGGGUG AUCGGCCACA CUG GGACUG AGACACGGCC CAGACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCCGCAA UGGACGAAAG UCUGACGGAG CAAC GCCGC GUGUAUGAAG AAGGUUUUCG GAUCGUAAAG UACUGUUGUU AGAGAAGAAC AAGGAUAAGA GUAACUGCUU GUCCC UUGA CGGUAUCUAA CCAGAAAGCC ACGGCUAACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGU GGCAAGCGUU GUCCGG AUU UAUUGGGCGU AAAGCGCGCG CAGGCGGUCU UUUAAGUCUG AUGUGAAAGC CCCCGGCUUA ACCGGGGAGG GUCAUUG GA AACUGGAAGA CUGGAGUGCA GAAGAGGAGA GUGGAAUUCC ACGUGUAGCG GUGAAAUGCG UAGAUAUGUG GAGGAACA C CAGUGGCGAA GGCGACUCUC UGGUCUGUAA CUGACGCUGA GGCGCGAAAG CGUGGGGAGC AAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA AGUGUUAGGG GGUUUCCGCC CCUUAGUGCU GCAGCUAACG CAUUAAGCAC UCCGCCUGG GGAGUACGAC CGCAAGGUUG AAACUCAAAG GAAUUGACGG GGGCCCGCAC AAGCGGUGGA GCAUGUGGUU U AAUUCGAA GCAACGCGAA GAACCUUACC AGGUCUUGAC AUCCUUUGAC CACUCUGGAG ACAGAGCUUU CCCUUCGGGG AC AAAGUGA CAGGUGGUGC AUGGUUGUCG UCAGCUCGUG UCGUGAGAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUG AUUUUA GUUGCCAGCA UUUAGUUGGG CACUCUAAAG UGACUGCCGG UGCAAGCCGG AGGAAGGUGG GGAUGACGUC AAAU CAUCA UGCCCCUUAU GACCUGGGCU ACACACGUGC UACAAUGGAU AGUACAAAGG GUCGCGAAGC CGCGAGGUGG AGCUA AUCC CAUAAAACUA UUCUCAGUUC GGAUUGUAGG CUGCAACUCG CCUACAUGAA GCCGGAAUCG CUAGUAAUCG UGGAUC AGC AUGCCACGGU GAAUACGUUC CCGGGCCUUG UACACACCGC CCGUCACACC ACGAGAGUUU GUAACACCCG AAGUCGG UA GGGUAACCUU UAUGGAGCCA GCCGCCGAAG GUGGGACAGA UAAUUGGGGU GAAGUCGUAA CAAGGUAGCC GUAUCGGA A GGUGCGGCUG GAUCACCUCC U

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Macromolecule #32: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Listeria monocytogenes EGD-E (bacteria)
Molecular weightTheoretical: 36.692707 KDa
SequenceString:
CUGGUAGUUA UGGCGAGAAG GUCACACCCG UUCCCAUCCC GAACACGGUA GUUAAGCUUC UCUGCGCCAA UGGUAGUUGG GGGCUUCCC CCUGCGAGAG UAGGUCGCUG CCGGG

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Macromolecule #53: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 53 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 143 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #55: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 55 / Number of copies: 2 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE / Spermidine

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Macromolecule #56: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 56 / Number of copies: 21 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #57: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 57 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: -1.9000000000000001 µm / Nominal defocus min: -0.7000000000000001 µm / Nominal magnification: 165000
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Number grids imaged: 1 / Average electron dose: 26.3 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 83340
CTF correctionSoftware - Name: Gctf
Startup modelType of model: INSILICO MODEL
In silico model: Generated by RELION using 70S particles from 2D classification.
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 45548
FSC plot (resolution estimation)

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