+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1h8b | ||||||
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タイトル | EF-hands 3,4 from alpha-actinin / Z-repeat 7 from titin | ||||||
要素 |
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キーワード | STRUCTURAL PROTEIN / Z-DISK STRUCTURAL COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 actin filament uncapping / FATZ binding / titin Z domain binding / phospholipase C-activating angiotensin-activated signaling pathway / positive regulation of endocytic recycling / positive regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transporter activity / telethonin binding / positive regulation of cation channel activity / negative regulation of protein localization to cell surface ...actin filament uncapping / FATZ binding / titin Z domain binding / phospholipase C-activating angiotensin-activated signaling pathway / positive regulation of endocytic recycling / positive regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transporter activity / telethonin binding / positive regulation of cation channel activity / negative regulation of protein localization to cell surface / LIM domain binding / postsynaptic actin cytoskeleton / microspike assembly / positive regulation of potassium ion transport / muscle cell development / focal adhesion assembly / Striated Muscle Contraction / Assembly and cell surface presentation of NMDA receptors / cardiac muscle cell development / Nephrin family interactions / structural constituent of muscle / intracellular non-membrane-bounded organelle / sarcomere organization / cortical actin cytoskeleton / Negative regulation of NMDA receptor-mediated neuronal transmission / Unblocking of NMDA receptors, glutamate binding and activation / pseudopodium / negative regulation of potassium ion transport / Long-term potentiation / postsynaptic density, intracellular component / titin binding / phosphatidylinositol-4,5-bisphosphate binding / cytoskeletal protein binding / Ras activation upon Ca2+ influx through NMDA receptor / regulation of membrane potential / platelet alpha granule lumen / nuclear receptor coactivator activity / filopodium / protein localization to plasma membrane / cell projection / actin filament / postsynaptic density membrane / Z disc / actin filament binding / integrin binding / cell junction / Platelet degranulation / RAF/MAP kinase cascade / actin cytoskeleton organization / regulation of apoptotic process / transmembrane transporter binding / dendritic spine / cytoskeleton / cell adhesion / protein domain specific binding / focal adhesion / glutamatergic synapse / calcium ion binding / extracellular exosome / extracellular region / identical protein binding / cytosol 類似検索 - 分子機能 | ||||||
生物種 | HOMO SAPIENS (ヒト) ORYCTOLAGUS CUNICULUS (ウサギ) | ||||||
手法 | 溶液NMR / TORSION ANGLE DYNAMICS, CARTESIAN DYANMICS | ||||||
データ登録者 | Atkinson, R.A. / Joseph, C. / Kelly, G. / Muskett, F.W. / Frenkiel, T.A. / Nietlispach, D. / Pastore, A. | ||||||
引用 | ジャーナル: Nat.Struct.Biol. / 年: 2001 タイトル: Ca2+-Independent Binding of an EF-Hand Domain to a Novel Motif in the Alpha-Actinin-Titin Complex 著者: Atkinson, R.A. / Joseph, C. / Kelly, G. / Muskett, F.W. / Frenkiel, T.A. / Nietlispach, D. / Pastore, A. #1: ジャーナル: Biochemistry / 年: 2000 タイトル: The Binding of Alpha-Actinin to Titin: Implications for Z-Disk Assembly 著者: Atkinson, R.A. / Joseph, C. / Piaz, F.D. / Birolo, L. / Stier, G. / Pucci, P. / Pastore, A. | ||||||
履歴 |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1h8b.cif.gz | 845.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1h8b.ent.gz | 713.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1h8b.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1h8b_validation.pdf.gz | 355.5 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1h8b_full_validation.pdf.gz | 779.7 KB | 表示 | |
XML形式データ | 1h8b_validation.xml.gz | 58.5 KB | 表示 | |
CIF形式データ | 1h8b_validation.cif.gz | 90.5 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/h8/1h8b ftp://data.pdbj.org/pub/pdb/validation_reports/h8/1h8b | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 8077.011 Da / 分子数: 1 / 断片: EF-HANDS 3&4 RESIDUE 822-894 / 由来タイプ: 組換発現 / 由来: (組換発現) HOMO SAPIENS (ヒト) / 組織: MUSCLE / 発現宿主: ESCHERICHIA COLI (大腸菌) / 参照: UniProt: P35609 |
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#2: タンパク質 | 分子量: 5667.321 Da / 分子数: 1 / 断片: Z-REPEAT 7 RESIDUES 648-698 / 由来タイプ: 組換発現 / 由来: (組換発現) ORYCTOLAGUS CUNICULUS (ウサギ) / 組織: MUSCLE / 発現宿主: ESCHERICHIA COLI (大腸菌) / 参照: UniProt: O97791 |
構成要素の詳細 | CHAIN A ENGINEERED MUTATION THR822MET, MODIFIED FOR EXPRESSION F-ACTIN CROSS-LINKING PROTEIN IS ...CHAIN A ENGINEERED |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験 | タイプ: SEE PUBLICATION |
NMR実験の詳細 | Text: THE STRUCTURE OF THE COMPLEX WAS DETERMINED USING CONSTRAINTS FROM 15N-EDITED NOESY, 13C-EDITED NOESY AND F1-FILTERED/F3-EDITED NOESY SPECTRA, RECORDED ON 13C, 15N-LABELLED SAMPLES IN WHICH ...Text: THE STRUCTURE OF THE COMPLEX WAS DETERMINED USING CONSTRAINTS FROM 15N-EDITED NOESY, 13C-EDITED NOESY AND F1-FILTERED/F3-EDITED NOESY SPECTRA, RECORDED ON 13C, 15N-LABELLED SAMPLES IN WHICH ONLY ONE OF THE TWO COMPONENTS WAS LABELLED |
-試料調製
詳細 | 内容: 0.7 MM COMPLEX |
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試料状態 | イオン強度: 20 mM / pH: 6.6 / 圧: 1 atm / 温度: 300 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: TORSION ANGLE DYNAMICS, CARTESIAN DYANMICS / ソフトェア番号: 1 詳細: REFINEMENT DETAILS MAY BE FOUND IN THE JRNL CITATION ABOVE THE FIRST RESIDUE OF THE NATURAL SEQUENCE OF ALPHA-ACTININ EF34 IS REPLACED BY A MET. THIS IS PRECEDED BY THE DIPEPTIDE GLY-ALA. ...詳細: REFINEMENT DETAILS MAY BE FOUND IN THE JRNL CITATION ABOVE THE FIRST RESIDUE OF THE NATURAL SEQUENCE OF ALPHA-ACTININ EF34 IS REPLACED BY A MET. THIS IS PRECEDED BY THE DIPEPTIDE GLY-ALA. THERE ARE NO DISTANCE CONSTRAINTS FOR THESE TWO RESIDUES WHICH ARE OMITTED FROM THE STRUCTURE CALCULATION. THE FIRST RESIDUE OF THE NATURAL SEQUENCE OF TITIN ZR7 IS REPLACED BY A MET. THIS IS PRECEDED BY THE DIPEPTIDE GLY-ALA. THERE ARE NO DISTANCE CONSTRAINTS FOR THE N-TERMINAL 8 RESIDUES AND THE C-TERMINAL 22 RESIDUES WHICH ARE OMITTED FROM THE STRUCTURE CALCULATION. | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LOWEST ENERGIES / 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 30 |