+Open data
-Basic information
Entry | Database: PDB / ID: 2k7b | ||||||
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Title | NMR structure of Mg2+-bound CaBP1 N-domain | ||||||
Components | Calcium-binding protein 1 | ||||||
Keywords | METAL BINDING PROTEIN / EF-hand / calcium / CaBP1 / Alternative splicing / Cell membrane / Cytoplasm / Cytoskeleton / Lipoprotein / Membrane / Myristate | ||||||
Function / homology | Function and homology information enzyme inhibitor activity / Sensory processing of sound by inner hair cells of the cochlea / response to stimulus / nuclear localization sequence binding / negative regulation of protein import into nucleus / calcium channel regulator activity / visual perception / calcium-dependent protein binding / cell cortex / postsynaptic density ...enzyme inhibitor activity / Sensory processing of sound by inner hair cells of the cochlea / response to stimulus / nuclear localization sequence binding / negative regulation of protein import into nucleus / calcium channel regulator activity / visual perception / calcium-dependent protein binding / cell cortex / postsynaptic density / cytoskeleton / Golgi membrane / calcium ion binding / perinuclear region of cytoplasm / extracellular space / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Ames, J. | ||||||
Citation | Journal: To be Published Title: NMR structure of Mg2+-bound CaBP1 N-domain Authors: Ames, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2k7b.cif.gz | 363.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2k7b.ent.gz | 298.6 KB | Display | PDB format |
PDBx/mmJSON format | 2k7b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k7/2k7b ftp://data.pdbj.org/pub/pdb/validation_reports/k7/2k7b | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8957.129 Da / Num. of mol.: 1 / Fragment: UNP residues 76 to 151 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CABP1 / Plasmid: pET3b / Production host: Escherichia coli (E. coli) / References: UniProt: Q9NZU7 |
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#2: Chemical | ChemComp-MG / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.0 mM [U-99% 13C; U-99% 15N] CaBP1, 10 mM TRIS, 50 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.05 / pH: 7.4 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: xplor-NIH and YASAP protocol | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 15 |