[English] 日本語
Yorodumi- PDB-1uss: YEAST HISTONE H1 GLOBULAR DOMAIN II, HHO1P GII, SOLUTION NMR STRU... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1uss | ||||||
---|---|---|---|---|---|---|---|
Title | YEAST HISTONE H1 GLOBULAR DOMAIN II, HHO1P GII, SOLUTION NMR STRUCTURES | ||||||
Components | HISTONE H1 | ||||||
Keywords | DNA BINDING PROTEIN / LINKER HISTONE / DNA BINDING DOMAIN | ||||||
Function / homology | Function and homology information negative regulation of DNA recombination / supercoiled DNA binding / chromosome condensation / phosphate ion binding / nucleosomal DNA binding / protein-DNA complex / chromatin DNA binding / structural constituent of chromatin / nucleosome / nucleosome assembly ...negative regulation of DNA recombination / supercoiled DNA binding / chromosome condensation / phosphate ion binding / nucleosomal DNA binding / protein-DNA complex / chromatin DNA binding / structural constituent of chromatin / nucleosome / nucleosome assembly / double-stranded DNA binding / nucleic acid binding / molecular adaptor activity / regulation of DNA-templated transcription / chromatin / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, BOND ANGLE GEOMETRY | ||||||
Authors | Ali, T. / Coles, P. / Stevens, T.J. / Stott, K. / Thomas, J.O. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: Two Homologous Domains of Similar Structure But Different Stability in the Yeast Linker Histone, Hho1P Authors: Ali, T. / Coles, P. / Stevens, T.J. / Stott, K. / Thomas, J.O. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1uss.cif.gz | 279.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1uss.ent.gz | 231.7 KB | Display | PDB format |
PDBx/mmJSON format | 1uss.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1uss_validation.pdf.gz | 401.8 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1uss_full_validation.pdf.gz | 466 KB | Display | |
Data in XML | 1uss_validation.xml.gz | 17.4 KB | Display | |
Data in CIF | 1uss_validation.cif.gz | 27.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/us/1uss ftp://data.pdbj.org/pub/pdb/validation_reports/us/1uss | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 9746.461 Da / Num. of mol.: 1 / Fragment: GLOBULAR DOMAIN II, RESIDUES 171-258 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PET17B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P53551 |
---|---|
Compound details | COULD ACT AS AN H1-TYPE LINKER HISTONE. HAS BEEN SHOWN TO BIND DNA. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|
-Sample preparation
Sample conditions | pH: 7.0 / Pressure: 1 atm / Temperature: 288 K |
---|
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: DISTANCE GEOMETRY, BOND ANGLE GEOMETRY / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: MINIMUM ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 10 |