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Yorodumi- PDB-1ust: YEAST HISTONE H1 GLOBULAR DOMAIN I, HHO1P GI, SOLUTION NMR STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ust | ||||||
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Title | YEAST HISTONE H1 GLOBULAR DOMAIN I, HHO1P GI, SOLUTION NMR STRUCTURES | ||||||
Components | HISTONE H1 | ||||||
Keywords | DNA BINDING PROTEIN / LINKER HISTONE / DNA BINDING DOMAIN / WINGED HELIX FOLD | ||||||
Function / homology | Function and homology information sporulation / negative regulation of DNA recombination / regulation of double-strand break repair / supercoiled DNA binding / chromosome condensation / phosphate ion binding / nucleosomal DNA binding / protein-DNA complex / chromatin DNA binding / structural constituent of chromatin ...sporulation / negative regulation of DNA recombination / regulation of double-strand break repair / supercoiled DNA binding / chromosome condensation / phosphate ion binding / nucleosomal DNA binding / protein-DNA complex / chromatin DNA binding / structural constituent of chromatin / nucleosome / nucleosome assembly / double-stranded DNA binding / nucleic acid binding / molecular adaptor activity / regulation of DNA-templated transcription / chromatin / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, BOND ANGLE GEOMETRY | ||||||
Authors | Ali, T. / Coles, P. / Stevens, T.J. / Stott, K. / Thomas, J.O. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: Two Homologous Domains of Similar Structure But Different Stability in the Yeast Linker Histone, Hho1P Authors: Ali, T. / Coles, P. / Stevens, T.J. / Stott, K. / Thomas, J.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ust.cif.gz | 287.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ust.ent.gz | 238 KB | Display | PDB format |
PDBx/mmJSON format | 1ust.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/us/1ust ftp://data.pdbj.org/pub/pdb/validation_reports/us/1ust | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10277.848 Da / Num. of mol.: 1 / Fragment: GLOBULAR DOMAIN I, RESIDUES 38-130 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PET17B / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P53551 |
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Compound details | COULD ACT AS AN H1-TYPE LINKER HISTONE. HAS BEEN SHOWN TO BIND DNA. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Sample conditions | pH: 7 / Pressure: 1 atm / Temperature: 288 K |
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-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY, BOND ANGLE GEOMETRY / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: MINIMUM ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 10 |