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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-9944 | ||||||||||||
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Title | Human MCU-EMRE complex | ||||||||||||
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![]() | ion channel / Mitochondrial Ca2+ uptake / MCU / TRANSPORT PROTEIN | ||||||||||||
Function / homology | ![]() uniporter activity / Processing of SMDT1 / mitochondrial calcium ion transmembrane transport / uniplex complex / Mitochondrial calcium ion transport / positive regulation of mitochondrial calcium ion concentration / mitochondrial calcium ion homeostasis / channel activator activity / calcium import into the mitochondrion / positive regulation of neutrophil chemotaxis ...uniporter activity / Processing of SMDT1 / mitochondrial calcium ion transmembrane transport / uniplex complex / Mitochondrial calcium ion transport / positive regulation of mitochondrial calcium ion concentration / mitochondrial calcium ion homeostasis / channel activator activity / calcium import into the mitochondrion / positive regulation of neutrophil chemotaxis / positive regulation of mitochondrial fission / calcium channel complex / Mitochondrial protein degradation / calcium-mediated signaling / calcium channel activity / positive regulation of insulin secretion / glucose homeostasis / protein complex oligomerization / protein-macromolecule adaptor activity / mitochondrial inner membrane / mitochondrial matrix / mitochondrion / nucleoplasm / identical protein binding Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.27 Å | ||||||||||||
![]() | Zhuo W / Zhou H | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of intact human MCU supercomplex with the auxiliary MICU subunits. Authors: Wei Zhuo / Heng Zhou / Runyu Guo / Jingbo Yi / Laixing Zhang / Lei Yu / Yinqiang Sui / Wenwen Zeng / Peiyi Wang / Maojun Yang / ![]() | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 8.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.4 KB 12.4 KB | Display Display | ![]() |
Images | ![]() | 85.6 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6k7xMC ![]() 9945C ![]() 6k7yC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.091 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Human MCU-EMRE complex
Entire | Name: Human MCU-EMRE complex |
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Components |
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-Supramolecule #1: Human MCU-EMRE complex
Supramolecule | Name: Human MCU-EMRE complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Calcium uniporter protein, mitochondrial
Macromolecule | Name: Calcium uniporter protein, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 32.300203 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: DDVTVVYQNG LPVISVRLPS RRERCQFTLK PISDSVGVFL RQLQEEDRGI DRVAIYSPDG VRVAASTGID LLLLDDFKLV INDLTYHVR PPKRDLLSHE NAATLNDVKT LVQQLYTTLC IEQHQLNKER ELIERLEDLK EQLAPLEKVR IEISRKAEKR T TLVLWGGL ...String: DDVTVVYQNG LPVISVRLPS RRERCQFTLK PISDSVGVFL RQLQEEDRGI DRVAIYSPDG VRVAASTGID LLLLDDFKLV INDLTYHVR PPKRDLLSHE NAATLNDVKT LVQQLYTTLC IEQHQLNKER ELIERLEDLK EQLAPLEKVR IEISRKAEKR T TLVLWGGL AYMATQFGIL ARLTWWEYSW DIMEPVTYFI TYGSAMAMYA YFVMTRQEYV YPEARDRQYL LFFHKGAKKS RF DLEKYNQ LKDAIAQAEM DLKRLRDPLQ VHLPLRQIG UniProtKB: Calcium uniporter protein, mitochondrial |
-Macromolecule #2: Essential MCU regulator, mitochondrial
Macromolecule | Name: Essential MCU regulator, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 8 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 5.993192 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: VIVTRSGAIL PKPVKMSFGL LRVFSIVIPF LYVGTLISKN FAALLEEHDI FVPE UniProtKB: Essential MCU regulator, mitochondrial |
-Macromolecule #3: CARDIOLIPIN
Macromolecule | Name: CARDIOLIPIN / type: ligand / ID: 3 / Number of copies: 8 / Formula: CDL |
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Molecular weight | Theoretical: 1.464043 KDa |
Chemical component information | ![]() ChemComp-CDL: |
-Macromolecule #4: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,...
Macromolecule | Name: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL type: ligand / ID: 4 / Number of copies: 16 / Formula: PLX |
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Molecular weight | Theoretical: 767.132 Da |
Chemical component information | ![]() ChemComp-PLX: |
-Macromolecule #5: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 5 / Number of copies: 6 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Applied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.27 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 179468 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-6k7x: |