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- EMDB-9678: Photosystem I of Chlamydomonas reinhardtii -

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Basic information

Entry
Database: EMDB / ID: EMD-9678
TitlePhotosystem I of Chlamydomonas reinhardtii
Map data
Sample
  • Complex: Chlamydomonas reinhardtii PSI with eight light harvesting antennae
    • Protein or peptide: x 17 types
  • Ligand: x 11 types
Function / homology
Function and homology information


photosynthesis, light harvesting in photosystem I / chloroplast thylakoid lumen / photosynthesis, light harvesting / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chlorophyll binding / chloroplast thylakoid membrane ...photosynthesis, light harvesting in photosystem I / chloroplast thylakoid lumen / photosynthesis, light harvesting / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chlorophyll binding / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / membrane => GO:0016020 / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI ...Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Chlorophyll A-B binding protein, plant and chromista / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Chlorophyll a-b binding protein, chloroplastic / Photosystem I reaction center subunit VIII / PSI subunit V / Chlorophyll a-b binding protein, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit IX ...Chlorophyll a-b binding protein, chloroplastic / Photosystem I reaction center subunit VIII / PSI subunit V / Chlorophyll a-b binding protein, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit IV, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center / Chlorophyll a-b binding protein, chloroplastic / Photosystem I reaction center subunit II, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic / Chlorophyll a-b binding protein, chloroplastic
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.89 Å
AuthorsPan X / Ma J / Su X / Liu Z / Zhang X / Li M
CitationJournal: Nat Plants / Year: 2019
Title: Antenna arrangement and energy transfer pathways of a green algal photosystem-I-LHCI supercomplex.
Authors: Xiaodong Su / Jun Ma / Xiaowei Pan / Xuelin Zhao / Wenrui Chang / Zhenfeng Liu / Xinzheng Zhang / Mei Li /
Abstract: During oxygenic photosynthesis, photosystems I and II (PSI and PSII) are essential for light-driven electron transport. Excitation energy transfer in PSI occurs extremely quickly, making it an ...During oxygenic photosynthesis, photosystems I and II (PSI and PSII) are essential for light-driven electron transport. Excitation energy transfer in PSI occurs extremely quickly, making it an efficient energy converter. In the alga Chlamydomonas reinhardtii (Cr), multiple units of light-harvesting complex I (LHCI) bind to the PSI core and function as peripheral antennae, forming a PSI-LHCI supercomplex. CrPSI-LHCI shows significantly larger antennae compared with plant PSI-LHCI while maintaining highly efficient energy transfer from LHCI to PSI. Here, we report structures of CrPSI-LHCI, solved by cryo-electron microscopy, revealing that up to ten LHCIs are associated with the PSI core. The structures provide detailed information about antenna organization and pigment arrangement within the supercomplexes. Highly populated and closely associated chlorophylls in the antennae explain the high efficiency of light harvesting and excitation energy transfer in CrPSI-LHCI.
History
Header (metadata) releaseJun 20, 2018-
Map releaseJun 20, 2018-
DepositionOct 10, 2018-
UpdateMay 1, 2019-
Current statusMay 1, 2019Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6ijj
  • Surface level: 0.07
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9678.png

Downloads & links

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Map

FileDownload / File: emd_9678.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.07 / Movie #1: 0.07
Minimum - Maximum-0.1588642 - 0.39064652
Average (Standard dev.)0.003640059 (±0.02154177)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-100-100-100
Dimensions200200200
Spacing200200200
CellA=B=C: 208.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.041.041.04
M x/y/z200200200
origin x/y/z0.0000.0000.000
length x/y/z208.000208.000208.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-100-100-100
NC/NR/NS200200200
D min/max/mean-0.1590.3910.004

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Supplemental data

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Sample components

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Entire : Chlamydomonas reinhardtii PSI with eight light harvesting antennae

EntireName: Chlamydomonas reinhardtii PSI with eight light harvesting antennae
Components
  • Complex: Chlamydomonas reinhardtii PSI with eight light harvesting antennae
    • Protein or peptide: PsaA
    • Protein or peptide: PsaB
    • Protein or peptide: PsaC
    • Protein or peptide: PsaD
    • Protein or peptide: PsaE
    • Protein or peptide: PsaF
    • Protein or peptide: PsaI
    • Protein or peptide: PsaJ
    • Protein or peptide: PsaK
    • Protein or peptide: PsaL
    • Protein or peptide: Lhca1
    • Protein or peptide: Lhca3
    • Protein or peptide: Lhca4
    • Protein or peptide: Lhca5
    • Protein or peptide: Lhca6
    • Protein or peptide: Lhca7
    • Protein or peptide: Lhca8
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONEPhytomenadione
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: DODECYL-ALPHA-D-MALTOSIDE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

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Supramolecule #1: Chlamydomonas reinhardtii PSI with eight light harvesting antennae

SupramoleculeName: Chlamydomonas reinhardtii PSI with eight light harvesting antennae
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#17
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: PsaA

MacromoleculeName: PsaA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 83.239203 KDa
SequenceString: MTISTPEREA KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLS GMYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL Y TTAIGGLV ...String:
MTISTPEREA KKVKIAVDRN PVETSFEKWA KPGHFSRTLS KGPNTTTWIW NLHADAHDFD SHTSDLEEIS RKVFSAHFGQ LGIIFIWLS GMYFHGARFS NYEAWLSDPT HIKPSAQVVW PIVGQEILNG DVGGGFQGIQ ITSGFFQLWR ASGITSELQL Y TTAIGGLV MAAAMFFAGW FHYHKAAPKL EWFQNVESML NHHLGGLLGL GSLAWAGHQI HVSLPVNKLL DAGVDPKEIP LP HDLLLNR AIMADLYPSF AKGIAPFFTL NWSEYSDFLT FKGGLNPVTG GLWLSDTAHH HVAIAVLFLV AGHMYRTNWG IGH SMKEIL EAHRGPFTGE GHVGLYEILT TSWHAQLAIN LALFGSLSII VAHHMYAMPP YPYLATDYGT QLSLFTHHTW IGGF CIVGA GAHAAIFMVR DYDPTNNYNN LLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMSAL GRPQDMFSDT AIQLQ PVFA QWIQNTHFLA PQLTAPNALA ATSLTWGGDL VAVGGKVAMM PISLGTSDFM VHHIHAFTIH VTVLILLKGV LFARSS RLI PDKANLGFRF PCDGPGRGGT CQVSAWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVT ASGVSHITGG NFAQSAN TI NGWLRDFLWA QSSQVIQSYG SALSAYGLIF LGAHFVWAFS LMFLFSGRGY WQELIESIVW AHNKLKVAPA IQPRALSI T QGRAVGVAHY LLGGIATTWS FFLARIISVG

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Macromolecule #2: PsaB

MacromoleculeName: PsaB / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 82.184266 KDa
SequenceString: MATKLFPKFS QGLAQDPTTR RIWYGLAMAH DFESHDGMTE ENLYQKIFAS HFGQLSIIFL WTSGNLFHVA WQGNFEQWVT DPVHIRPIA HAIWDPHFGQ PAVEAFTRGG ASGPVNISTS GVYQWWYTIG MRTNQDLYVG SVFLALVSAI FLFAGWLHLQ P NFQPSLSW ...String:
MATKLFPKFS QGLAQDPTTR RIWYGLAMAH DFESHDGMTE ENLYQKIFAS HFGQLSIIFL WTSGNLFHVA WQGNFEQWVT DPVHIRPIA HAIWDPHFGQ PAVEAFTRGG ASGPVNISTS GVYQWWYTIG MRTNQDLYVG SVFLALVSAI FLFAGWLHLQ P NFQPSLSW FKDAESRLNH HLSGLFGVSS LAWTGHLVHV AIPESRGQHV GWDNFLSVLP HPQGLTPFFT GNWAAYAQSP DT ASHVFGT AQGSGQAILT FLGGFHPQTQ SLWLTDMAHH HLAIAVIFIV AGHMYRTNFG IGHRMQAILE AHTPPSGSLG AGH KGLFDT VNNSLHFQLG LALASVGTIT SLVAQHMYSL PPYAFQAIDF TTQAALYTHH QYIAGFIMCG AFAHGAIFFI RDYD PEQNK GNVLARMLDH KEALISHLSW VSLFLGFHTL GLYVHNDVMQ AFGTPEKQIL IEPVFAQWIQ AAHGKALYGF DFLLS SKTS AAFANGQSLW LPGWLDAINN NQNSLFLTIG PGDFLVHHAI ALGLHTTTLI LVKGALDARG SKLMPDKKDF GYSFPC DGP GRGGTCDISA YDAFYLAVFW MLNTIGWVTF YWHWKHLTLW QGNVAQFDES STYLMGWLRD YLWLNSSQLI NGYNPFG MN SLSVWAWTFL FGHLIYATGF MFLISWRGYW QELIETLVWA HEKTPLANLV YWKDKPVALS IVQARLVGLA HFSVGYIF T YAAFLIASTS GRFG

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Macromolecule #3: PsaC

MacromoleculeName: PsaC / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 8.869325 KDa
SequenceString:
MAHIVKIYDT CIGCTQCVRA CPLDVLEMVP WDGCKASQMA SAPRTEDCVG CKRCETACPT DFLSVRVYLG SESTRSMGLS Y

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Macromolecule #4: PsaD

MacromoleculeName: PsaD / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.372887 KDa
SequenceString: MAVMMRTQAP AATRASSRVA VAARPAARRA VVVRAEAEAA PAAAKKAAEK PAWTVPTLNP DTPSPIFGGS TGGLLRKAQT EEFYVITWE AKKEQIFEMP TGGAAIMRQG PNLLKFGKKE QCLALTTQLR NKFKLTPCFY RVFPDGKVQY LHPADGVYPE K VNAGRVGA ...String:
MAVMMRTQAP AATRASSRVA VAARPAARRA VVVRAEAEAA PAAAKKAAEK PAWTVPTLNP DTPSPIFGGS TGGLLRKAQT EEFYVITWE AKKEQIFEMP TGGAAIMRQG PNLLKFGKKE QCLALTTQLR NKFKLTPCFY RVFPDGKVQY LHPADGVYPE K VNAGRVGA NQNMRRIGQN VNPIKVKFSG RMMSPAEI

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Macromolecule #5: PsaE

MacromoleculeName: PsaE / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 16.221703 KDa
SequenceString:
MVYAIPHVLC VDTTACHEWY QRRVHGGFQP RPRVTLRLSS PHQNRKMQAL SSRVNIAAKP QRAQRLVVRA EEVKAAPKKE VGPKRGSLV KILRPESYWF NQVGKVVSVD QSGVRYPVVV RFENQNYAGV TTNNYALDEV VAAK

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Macromolecule #6: PsaF

MacromoleculeName: PsaF / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 24.088936 KDa
SequenceString: MALTMRNPAV KASSRVAPSS RRALRVACQA QKNETASKVG TALAASALAA AVSLSAPSAA MADIAGLTPC SESKAYAKLE KKELKTLEK RLKQYEADSA PAVALKATME RTKARFANYA KAGLLCGNDG LPHLIADPGL ALKYGHAGEV FIPTFGFLYV A GYIGYVGR ...String:
MALTMRNPAV KASSRVAPSS RRALRVACQA QKNETASKVG TALAASALAA AVSLSAPSAA MADIAGLTPC SESKAYAKLE KKELKTLEK RLKQYEADSA PAVALKATME RTKARFANYA KAGLLCGNDG LPHLIADPGL ALKYGHAGEV FIPTFGFLYV A GYIGYVGR QYLIAVKGEA KPTDKEIIID VPLATKLAWQ GAGWPLAAVQ ELQRGTLLEK EENITVSPR

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Macromolecule #7: PsaI

MacromoleculeName: PsaI / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.586388 KDa
SequenceString:
MALRAVSAKS AVRPTVARAS VKPVAALKPA QKMALAGAAS VALLAASSSS AEASQVIATV ASAAQGYPFV PPSWAPSVFV PLTGLVLPA IAMATLFVYI EKEAPSS

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Macromolecule #8: PsaJ

MacromoleculeName: PsaJ / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 4.750509 KDa
SequenceString:
MKDFTTYLST APVIATIWFT FTAGLLIEIN RYFPDPLVFS F

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Macromolecule #9: PsaK

MacromoleculeName: PsaK / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 16.412221 KDa
SequenceString:
PRCICKMTAR QSRISHCPLR AFASSCTNLV HTCPRLSSIT SIYQPATMQA LATRPSAIRP TKAARRSSVV VRADGFIGSS TNLIMVAST TATLAAARFG LAPTVKKNTT AGLKLVDSKN SAGVISNDPA GFTIVDVLAM GAAGHGLGVG IVLGLKGIGA L

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Macromolecule #10: PsaL

MacromoleculeName: PsaL / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.203562 KDa
SequenceString: MPTKGSPLTG LITWAFLVSA AHTRTVDTRP RPRGIWRRAA VARRPVEERI ATGSSLAHTY HKMAVAMRSS TGLRATAARR QMPLGLGRV STVRVCAADT KKAQVISPVN GDPFVGMLET PVTSAPIVAT YLSNLPAYRT GVAPVLRGVE IGLAHGFLLA G PFIKLGPL ...String:
MPTKGSPLTG LITWAFLVSA AHTRTVDTRP RPRGIWRRAA VARRPVEERI ATGSSLAHTY HKMAVAMRSS TGLRATAARR QMPLGLGRV STVRVCAADT KKAQVISPVN GDPFVGMLET PVTSAPIVAT YLSNLPAYRT GVAPVLRGVE IGLAHGFLLA G PFIKLGPL RNVPETAEIA GSLSAAGLVL ILALCLSIYG SAQFQSTPSI GVKTLSGRSV ARDPLFSADG WSEFAAGFLV GG EAGVAWA YVCTQILPYY S

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Macromolecule #11: Lhca1

MacromoleculeName: Lhca1 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.135863 KDa
SequenceString: MGELSRADSL LRTITKPPIK MALSMRTLSA RTAAPRGFSG RRVAAVSNGS RVTMKAGNWL PGSDAPAWLP DDLPGNYGFD PLSLGKEPA SLKRFTESEV IHGRWAMLGV AGSLAVELLG YGNWYDAPLW AVNGGKATWF GIEVPFDLNA LLAFEFVAMA A AEGQRGDA ...String:
MGELSRADSL LRTITKPPIK MALSMRTLSA RTAAPRGFSG RRVAAVSNGS RVTMKAGNWL PGSDAPAWLP DDLPGNYGFD PLSLGKEPA SLKRFTESEV IHGRWAMLGV AGSLAVELLG YGNWYDAPLW AVNGGKATWF GIEVPFDLNA LLAFEFVAMA A AEGQRGDA GGVVYPGGAF DPLGFAKDSS KSGELKLKEI KNGRLAMVAF LGFVAQHAAT GKGPIAALGE HLANPWGANF AT NGISVPF F

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Macromolecule #12: Lhca3

MacromoleculeName: Lhca3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 32.629486 KDa
SequenceString: MMLTKSAQAA FSGKVARPAK ANRARLVCRA EEKSIAKVDR SKDQLYVGAS QSSLAYLDGS LPGDFGFDPL GLLDPVNSGG FIEPKWLQY SEVIHARWAM LGAAGCIAPE VLGAAGLIPD ATNIKWFESG VIPPAGSYNG YWADPYTIFF VEIVAMQFAE L RRLQDFRY ...String:
MMLTKSAQAA FSGKVARPAK ANRARLVCRA EEKSIAKVDR SKDQLYVGAS QSSLAYLDGS LPGDFGFDPL GLLDPVNSGG FIEPKWLQY SEVIHARWAM LGAAGCIAPE VLGAAGLIPD ATNIKWFESG VIPPAGSYNG YWADPYTIFF VEIVAMQFAE L RRLQDFRY PGSMGQQYFL GLEAIFKGSG DAAYPGGPFF NLFNLGKTEA AMKELKLKEI KNGRLAMLAM LGYGAQAVMT GK GPFQNLV EHLADPVNNN ILTNFAGRVS GSSQPWRPHG WWRRRYRSVA ALALIRNRSV C

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Macromolecule #13: Lhca4

MacromoleculeName: Lhca4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 31.46201 KDa
SequenceString: MYSALIPLLS SGINAEYMGV SRCSGKMAFV LAKSSAFGVA AKPVSRRSSV AVKASAVPEN VKEAREWIDA WKSKSGGAKR DAALPSWMP GADLPGYLNG TLPGDFGFDP LYLGQDPVKL KWYAQAELMN ARFAMLAVAG ILVPELLSNI GFSWPGAGVA W YDAGKFEY ...String:
MYSALIPLLS SGINAEYMGV SRCSGKMAFV LAKSSAFGVA AKPVSRRSSV AVKASAVPEN VKEAREWIDA WKSKSGGAKR DAALPSWMP GADLPGYLNG TLPGDFGFDP LYLGQDPVKL KWYAQAELMN ARFAMLAVAG ILVPELLSNI GFSWPGAGVA W YDAGKFEY FAPASSLFGV QMLLFAWVEI RRYQDFVKPG SANQDPIFTN NKLPDGNEPG YPGGIFDPFG WSKGDIKSLK LK EIKNGRL AMLAFAGFIG QAYTTGTTPL KNLSTHLADP WSTTVWQNDL ARL

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Macromolecule #14: Lhca5

MacromoleculeName: Lhca5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 30.15165 KDa
SequenceString: AVAWFFTPGH TNHQPAKMAA LMQKSALSRP ACSTRSSRRA VVVRAAADRK LWAPGVVAPE YLKGDLAGDY GWDPLGLGAD PTALKWYRQ SELQHARWAM LGVAGVLVQE IVKPDVYFYE AGLPQNLPEP FTNINMGGLL AWEFILMHWV EVRRWQDYKN F GSVNEDPI ...String:
AVAWFFTPGH TNHQPAKMAA LMQKSALSRP ACSTRSSRRA VVVRAAADRK LWAPGVVAPE YLKGDLAGDY GWDPLGLGAD PTALKWYRQ SELQHARWAM LGVAGVLVQE IVKPDVYFYE AGLPQNLPEP FTNINMGGLL AWEFILMHWV EVRRWQDYKN F GSVNEDPI FKGNKVPNPE MGYPGGIFDP FGFSKGNLKE LQTKEIKNGR LAMIAYMAFI LQAQATGKGP LAALSAHLSN PF GNNILKN IGTCTVPHSV DVQGLTIPLT CLWPGSQ

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Macromolecule #15: Lhca6

MacromoleculeName: Lhca6 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 35.122043 KDa
SequenceString: MDIRREWEWC RVPHLQEPCN PGREKKKKKK KKKKYSALIP LLLSTQSTWG VFAKSHYTVP KMMLVAKNAV AARPSARSAR RSVVAKASS RPLWLPGSTP PAHLKGDLPG DFGFDPLGLG ANAESLKWFK ESELVHSRWA MAAVAGILVQ EIVRPDVFWY N AGKEVESP ...String:
MDIRREWEWC RVPHLQEPCN PGREKKKKKK KKKKYSALIP LLLSTQSTWG VFAKSHYTVP KMMLVAKNAV AARPSARSAR RSVVAKASS RPLWLPGSTP PAHLKGDLPG DFGFDPLGLG ANAESLKWFK ESELVHSRWA MAAVAGILVQ EIVRPDVFWY N AGKEVESP LGPLGLLAVE FFLMHWVEVR RWQDLRKPGS VDQDPIFSQY KLPPHEVGYP GGVFAPFIPG DLAELKVKEI KN GRLAMLA FVGFVMAAQV TGKGPIAALQ EHLADPWGTT IFSKAAVVPG QAVAPPCKIP ASVSYKGIEI PTPCFLQGLW P

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Macromolecule #16: Lhca7

MacromoleculeName: Lhca7 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.248869 KDa
SequenceString: MALSMIAQRR AGAFSARQAP RAVRAQAAVR PVWFPGNPPP AHLDGSLAGD YGFDPLFLGQ EPQTLKWYVQ AELVHGRFAM LGAAGIILT SIGAKVGLGF PEWYDAGKVV VEKNNIDFPT LMVIQFYLMG WAETKRWYDF KNPGSQADGS FLGFTEEFKG L ENGYPGGR ...String:
MALSMIAQRR AGAFSARQAP RAVRAQAAVR PVWFPGNPPP AHLDGSLAGD YGFDPLFLGQ EPQTLKWYVQ AELVHGRFAM LGAAGIILT SIGAKVGLGF PEWYDAGKVV VEKNNIDFPT LMVIQFYLMG WAETKRWYDF KNPGSQADGS FLGFTEEFKG L ENGYPGGR FFDPMGLSRG DAAKYQEYKQ KEVKNGRLAM IACLGFAAQY AATGKGPLDN LADHLADPNH VNFATNGVSI PI A

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Macromolecule #17: Lhca8

MacromoleculeName: Lhca8 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.178635 KDa
SequenceString: GRQFAPRFLA LCLCRCCLRT EHGPHSPATM ALTMKRSGVA ARSASSRKSV VTCVARQSWL PGSQIPAHLD TPAAQALAGN FGFDPLGLG KDPVALRWYQ QAELIHCRTA MAGVAGILIP GLLTKAGALN VPEWYDAGKV AIENSFAPWG SLLAVQLFLC G FVEAKRWQ ...String:
GRQFAPRFLA LCLCRCCLRT EHGPHSPATM ALTMKRSGVA ARSASSRKSV VTCVARQSWL PGSQIPAHLD TPAAQALAGN FGFDPLGLG KDPVALRWYQ QAELIHCRTA MAGVAGILIP GLLTKAGALN VPEWYDAGKV AIENSFAPWG SLLAVQLFLC G FVEAKRWQ DIRKPGSQGE PGSFLGFEAS LKGTSELGYP GGPFDPLGLS KEADKWADWK LKEVKNGRLA MLAFLGFVAQ KY ATGAGPV DNLAAHLKDP WHVNYATNGV SLPFL

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Macromolecule #18: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 18 / Number of copies: 216 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

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Macromolecule #19: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 19 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

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Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 20 / Number of copies: 14 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #21: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 21 / Number of copies: 30 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #22: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 22 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #23: DODECYL-ALPHA-D-MALTOSIDE

MacromoleculeName: DODECYL-ALPHA-D-MALTOSIDE / type: ligand / ID: 23 / Number of copies: 7 / Formula: LMU
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

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Macromolecule #24: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 24 / Number of copies: 7 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #25: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 25 / Number of copies: 2 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

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Macromolecule #26: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 26 / Number of copies: 8 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

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Macromolecule #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 27 / Number of copies: 8 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

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Macromolecule #28: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...

MacromoleculeName: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL
type: ligand / ID: 28 / Number of copies: 2 / Formula: NEX
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: COMMON LINE
Final angle assignmentType: COMMON LINE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 58955

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