EMDB-9242: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)

Basic information

• Entry: Database: EMDB / ID: EMD-9242
• Title: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)
• Map data: Postprocessed map

Sample

• Complex: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)
  • RNA: x 4 types
  • Protein or peptide: x 79 types
• Ligand: x 3 types

Function / homology

Function:

Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / L13a-mediated translational silencing of Ceruloplasmin expression / Translation initiation complex formation / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Protein hydroxylation / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / RHO GTPases Activate Formins / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Separation of Sister Chromatids / TORC2 complex binding / erythrocyte homeostasis / Formation of the ternary complex, and subsequently, the 43S complex / cytoplasmic side of rough endoplasmic reticulum membrane / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / Ribosomal scanning and start codon recognition / Translation initiation complex formation / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition / activation-induced cell death of T cells / SARS-CoV-1 modulates host translation machinery / Peptide chain elongation / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / phagocytic cup / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / 90S preribosome / L13a-mediated translational silencing of Ceruloplasmin expression / TOR signaling / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / T cell proliferation involved in immune response / Major pathway of rRNA processing in the nucleolus and cytosol / protein-RNA complex assembly / erythrocyte development / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal small subunit export from nucleus / translation regulator activity / rough endoplasmic reticulum / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / gastrulation / MDM2/MDM4 family protein binding / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / translation initiation factor binding / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / erythrocyte differentiation

Domain/homology:

60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Ribosomal protein S26e signature. / metallochaperone-like domain / TRASH domain / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L19, eukaryotic / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein S3Ae, conserved site / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / Ribosomal protein S3Ae signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein S27e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein L30e signature 2. / Ribosomal protein S19A/S15e / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L30e, conserved site / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site

Component:

Large ribosomal subunit protein eL20 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL27 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein eS24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL10 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein eS21 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL37

• Biological species: Oryctolagus cuniculus (rabbit) / Rabbit (rabbit) / Bovine (cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 3.4 Å
• Authors: Brown A / Baird MR / Yip MCJ / Murray J / Shao S
• Citation: Journal: Elife / Year: 2018; Title: Structures of translationally inactive mammalian ribosomes.; Authors: Alan Brown / Matthew R Baird / Matthew Cj Yip / Jason Murray / Sichen Shao / ; Abstract: The cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. Here, we use electron cryomicroscopy to analyze inactive ribosomes isolated from mammalian reticulocytes, the penultimate stage of red blood cell differentiation. We identify two types of ribosomes that are translationally repressed by protein interactions. The first comprises ribosomes sequestered with elongation factor 2 (eEF2) by SERPINE mRNA binding protein 1 (SERBP1) occupying the ribosomal mRNA entrance channel. The second type are translationally repressed by a novel ribosome-binding protein, interferon-related developmental regulator 2 (IFRD2), which spans the P and E sites and inserts a C-terminal helix into the mRNA exit channel to preclude translation. IFRD2 binds ribosomes with a tRNA occupying a noncanonical binding site, the 'Z site', on the ribosome. These structures provide functional insights into how ribosomal interactions may suppress translation to regulate gene expression.

History

Deposition	Oct 19, 2018	-
Header (metadata) release	Nov 21, 2018	-
Map release	Nov 21, 2018	-
Update	May 15, 2019	-
Current status	May 15, 2019	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_9242.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Postprocessed map
• Voxel size: X=Y=Z: 1.34 Å

Density

Contour Level	By AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum	-0.385669 - 0.67855746
Average (Standard dev.)	0.0017560415 (±0.022576272)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 536.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.34	1.34	1.34
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	536.000	536.000	536.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	240	240	240
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.386	0.679	0.002

Supplemental data

Additional map: Pre-postprocessed map

• File: emd_9242_additional.map
• Annotation: Pre-postprocessed map

Half map: Half map 1

• File: emd_9242_half_map_1.map
• Annotation: Half map 1

Half map: Half map 2

• File: emd_9242_half_map_2.map
• Annotation: Half map 2

Sample components

Entire : Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)

• Entire: Name: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)

Components

• Complex: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)
  • RNA: 28S rRNA
  • RNA: 5S rRNA
  • RNA: 5.8S rRNA
  • Protein or peptide: uL2
  • Protein or peptide: uL3
  • Protein or peptide: uL4
  • Protein or peptide: uL18
  • Protein or peptide: eL6
  • Protein or peptide: uL30
  • Protein or peptide: eL8
  • Protein or peptide: uL6
  • Protein or peptide: uL16
  • Protein or peptide: uL5
  • Protein or peptide: eL13
  • Protein or peptide: eL14
  • Protein or peptide: eL15
  • Protein or peptide: uL13
  • Protein or peptide: uL22
  • Protein or peptide: eL18
  • Protein or peptide: eL19
  • Protein or peptide: eL20
  • Protein or peptide: eL21
  • Protein or peptide: eL22
  • Protein or peptide: uL14
  • Protein or peptide: eL24
  • Protein or peptide: uL23
  • Protein or peptide: uL24
  • Protein or peptide: eL27
  • Protein or peptide: uL15
  • Protein or peptide: eL29
  • Protein or peptide: eL30
  • Protein or peptide: eL31
  • Protein or peptide: eL32
  • Protein or peptide: eL33
  • Protein or peptide: eL34
  • Protein or peptide: uL29
  • Protein or peptide: eL36
  • Protein or peptide: eL37
  • Protein or peptide: eL38
  • Protein or peptide: eL39
  • Protein or peptide: eL40
  • Protein or peptide: eL41
  • Protein or peptide: eL42
  • Protein or peptide: eL43
  • Protein or peptide: eL28
  • Protein or peptide: uL10
  • Protein or peptide: uL11
  • Protein or peptide: eEF2
  • Protein or peptide: SERBP1
  • RNA: 18S rRNA
  • Protein or peptide: uS2
  • Protein or peptide: eS1
  • Protein or peptide: uS5
  • Protein or peptide: uS3
  • Protein or peptide: eS4
  • Protein or peptide: uS7
  • Protein or peptide: eS6
  • Protein or peptide: eS7
  • Protein or peptide: eS8
  • Protein or peptide: uS4
  • Protein or peptide: eS10
  • Protein or peptide: uS17
  • Protein or peptide: eS12
  • Protein or peptide: uS15
  • Protein or peptide: uS11
  • Protein or peptide: uS19
  • Protein or peptide: uS9
  • Protein or peptide: eS17
  • Protein or peptide: uS13
  • Protein or peptide: eS19
  • Protein or peptide: uS10
  • Protein or peptide: eS21
  • Protein or peptide: uS8
  • Protein or peptide: uS12
  • Protein or peptide: eS24
  • Protein or peptide: eS25
  • Protein or peptide: eS26
  • Protein or peptide: eS27
  • Protein or peptide: eS28
  • Protein or peptide: uS14
  • Protein or peptide: eS30
  • Protein or peptide: eS31
  • Protein or peptide: RACK1
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)

• Supramolecule: Name: Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state); type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#83
• Source (natural): Organism: Oryctolagus cuniculus (rabbit) / Location in cell: cytoplasm

Macromolecule #1: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 1.167366125 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGGGGGGC CCAAGUCCUU CUGAUCGAGG CCCAGCCCGU GGACGGUGUG AG(B9B)CCGGU A GCGGCCCCCG GCGCGCCGGG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUC CAUCUAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA (OMG)GGAAAGUUG AAAAGAACUU UGAAG (A2M)GAG AGUUCAAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CC CGGCGGC GCGGUCCGGC CGUGCCGGCG GGGCGGAUCU CCCGCUCCCG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGG CCGCCG CCGGGCGCAU UUCCACCGCG GCGGUGCGCC GCGACCGGCU CCGGGACGGC U(2MG)GGAAGGCC CCGGCGGGGA AGGUGGCCC GGGGGCCCCG GGUGUUACAG CCCCGCCCCG GCAGCAGCAG CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG A CCCGUGCC GCCGCGCUCU CCCCCCUGGG GGGGCCGGGC CACCCCUCCC ACGGCGCGAC CGCUGGGGCG GACUGUCCAG UG CGCCCCG GGCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCU ACCCAC CCGACCC(OMG)UC UUG(1MA)AAC(A2M)CG GACCAAGGAG UCUAACACG(P4U) GCGCGAGUCG GGGGCUAC G AAAGCCGCCG UGGCGCAAUG AAGGUGAAGG CCGGCGGCGC GCCGCCGGCC GAGGUGGGAU CCCGAGGCCU UCCAGUCCG CCGAGGG(B8Q)GC ACCACCGGCC CGUCUCGCCC GCCGCGCCGG GGAGGUGGAG CACGAGCGCA CGUGUUAG(2MG)A CC C(OMG)A(A2M)AGA UGGUGA(A2M)CUA UGCCUGGGCA GGGCGAAGCC AGAGGAAACU CUGGUG(B9B)AGG UCCG (PSU)AGCG GUCCUGACGU GCGAAUCG(7MG)U CGUCCGACCU GGGUAUAG(OMG)G GCGAAAGACU AAUCGAACCA UCU AGUAGC UGG(I4U)UCCCUC CAAAGUUUCC C(PSU)CAGGA(PSU)AG CUGGCGCUCU CCGCAGUUUU AUCCGGUAAA GC GAAUGAU UAGAGGUCUU GGGGCCGAAA CGAUCUCAAC CUAUUCUCAA ACUUUAAAU(E7G) GGUAAGAAGC CCGGCUCGC UGGUGUGGAG CCGGGCGUGG AAUGCGAGUG CCUAGUGGGC CAC(B8H)UUUGG(UR3) AAGC(A2M)GAACU GGCGCU (OMG)CG GGAUGAACCG AACGCCGGGU UAA(P7G)GCGCCC GAUGCCGACG CUCAUCAGAC CCCAGAAAAG GUGUUGGUU GAUAUAGACA GCAGGAUGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAA UGGAUG(OMG)CGC UGGAGCGUCG GGCCCAUACC CGGCCGUCGC UGGCAGUCGG CAGAGCGAGA AAGAGAG UG GCGCGGACGC UACGCCGCGA CGAGUAGGAG GGCCGCUGCG (E7G)UGAGCCUUG AAGCCUAGGG CGCGGGCCCG GGUG GAGCC GCCGCAGGUG CAGAUCUUGG UGGUAGU(A2M)(OMG)(OMC) AAAUAUUCAA ACGA(B8W)AACUU UGAAGGCCG AAGUGG(A2M)GAA GGGUUCCAUG UGAACAG(OMC)A(OMG) UUGAACAUGG GUCAGUCGGU CCUGAGAGAU GGGCGAGC G CCGUUCCGAA GGGACGGGCG AUGGCCUCCG UUGCCCUCAG CCGA(PSU)CGAAA GGGAGUCG(7MG)G UUCAGAUCCC C GAAUCCGG AGUGGCGGAG AUGGGCGCCG CGAGGCGUCC AGUGCGGUAA CGCGACCGAU CCCGGAGAAG CCGGCGGGAG CC CCGGGGA GAGUUCUCUU UUCUUUGUGA AGGGCAGGGC GCCCUGGAAU GGGUUCGCCC CGAGAGAGGG GCCCGUGCCU UGG AAAGCG UCGCGGUUCC GGCGGCGUCC GGUGAGCUCU CGCUGGCCCU UGAAAAUCCG GGG(B9B)AGAGGG UGUAAAUCUC GC(OMG)CCGGGC CGUACC(B9H)AUA UCCGCAGCAG GUCU(OMC)CAAGG UGAACAGCCU CUGGCAUGUU GGAACAAUG UAGGUAAGGG AAGUCGGCAA GC(OMC)GGAUCCG UAACUUCGGG AUAAGGAUUG GCUCUAAGGG CUGGCAGCCG ACUUAG AAC UGGUGCGGAC CAGGGGAAUC CGACUGUUUA AUUAAAACAA AGCAUCGCGA AGGCCCGCGG CGGGUGUUGA CGCGAUG UG AUUUCUGCC(OMC) AGUGCUCUGA AUG(PSU)CA(A2M)AGU G(A2M)AGAAA(PSU)UC AAUGAAGCGC GGGUAAAC G GCGGGAGUAA C(B8H)A(PSU)GACUCU CUUAAGGUAG C(5MC)AA(A2M)UGCCU C(OMG)UCAUCUAA UUAGUGACG CGCAUGAAUG GAUGA(A2M)CGAG AUUCCCACUG UCCCUACCUA CUAUCCAGCG AAACCAC(A2M)G(OMC) CAAGGGAA C G(P7G)GCUUGG(OMC)G GAAUCAGC(B8K)G (BGH)GAAAGAAGA (OMC)CCUGUUGAG CUUGACUCUA GUCUGGCAC GGUGAAGAGA CAUGAGAAGU GUAGAAUAAG UGGGAGGCCG CCGGUGAAAU ACCACUACUC UGAUCGUUUU UUCACUGACC CGG(5MU)GAGGC GGGGGGGCGA GCCGGGCUCU CGCUUCUGGC GCCAA(B8W)CGCC CGGCCGGCCG GGCGCGACCC GCU CCGGGG ACAGUGCCAG GUGGG(B8W)AGUU UGAC(I4U)G(OMG)GGC GGUACACCUG UCAAACGGUA (6MZ)CGCAGGU G UCCUAAGGCG AGCUCAGGGA GGACAGAAAC CUCCCGUGGA GCAGAAGGGC AAAAGCUCGC UUGA(PSU)CU(B8H)GA U UUUCAG(OMU)A CGAAUACAGA CCGUGAAAGC GGGGCCU(5MC)AC GAUCCUUCUG ACCUUUU(E6G)GG UUUUAAGCAG GA(OMG)(MHG)UGUCA GAAAAGUUAC CACAGGGAUA ACUGGC(PSU)UGU GGCGGCCA(1MA)G CGUUCAUAGC GACGU CGCU UUUUGA(PSU)CCU U(5MC)GA(PSU)GUCGG CUCUUCCUAU CAUUGU(B8W)AAG CAGAAUU(B8T)AC CAAGCG UU(OMG) GAUUG(PSU)UCAC CCACUAAUAG GGAACGUG(A2M)G CUGG(B8W)(UR3)(PSU)UAG A(OMC)CGUCGUG AGACAG(7MG)UUA GUUUUACCCU (M7A)CUGAUG(A2M)UG UGUUGUUGCC AUGGUAAUCC UGC(UR3)CAGUAC GAGA GGAAC CGCAGGU(OMU)CA (OMG)ACAU(PSU)UGGU GUA(PSU)(OMG)UGCUU GGCUGAGGAG CCAAUGGGGC GAAGC UAC(B8T) AUCUGUGGGA UUAUGACU(B8K)A ACGCCUCUAA GUCAGAAUCC CGCCCAGGCG GAACGAUAGC GCCGGAGC C UCGGUUGGCC UCGGAUAGCC GGUCCCCCGC CGGGGUCCGG U(OMG)C(2MG)GAGAGC CCUUCGUCCC GGGAAACGGG G CGGCCGGA AAGGGGGCCG CCCUCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU GGCGCUAAAC CAUUCGUAGA CG ACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUGCUGCG AUCUAUUGAA AGUCAGCCCU CGACACAAGG GUU UGU

Macromolecule #2: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 38.691914 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

Macromolecule #3: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 48.559699 KDa

Sequence

String:

CGACUCUUAG CGG(OMU)GGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGC AGG UUGAUCAUCG ACACUUCGAA CGCACUUGCG GCCCCGGGUU CCUCCCGGGG CUACGCCUGU CUGAGCGUCG CU

Macromolecule #50: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 50 / Number of copies: 1
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 548.638062 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGU ACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGU(OMU)CC(PSU) U(OMU)GGUCGCUC GUACUUGGAU AACUG UGGU A(A2M)UUCUAG(A2M)G CUAAUA(OMC)AUG CCGACGGGCG CUGACCCCCU UCGCGGGGGG GAUGCGUGCA UUUA UCAGU GGUGACUCUA GAUAACCUCG GGCCGAUCGC ACGCCCGGCG GCGACGACCC AUUCGAACGU CUGCCCUAUC AACUU UCGA UGGUAGUCGC CGUGCCUACC AUGGUGACCA CGGGUGACGG GGAAUCAGGG UUCGAUUCCG GAGAGGGAGC CUGAGA AAC GGCUACCACA UCCAAGGAAG GCAGCAGGCG CGC(A2M)AAUUAC CCACUCCCGA CCCGGGGA(OMG)G UAGUGA (OMC)GA AAAAUAACAA UACAGGACUC UUUCGAGGCC CUGUAAUUGG AAUGAGUC(E3C)A CUUUAAAUCC UUUAACGAG GAUCCAUUGG AGGGCAAGUC UGG(PSU)GCCAGC AGCCGCGGUA AUUCCAGCUC CAAUA(OMG)CGUA UAUUAAAGUU GC UGCAGUU (A2M)AAAAGCUCG UAGUU(OMG)GAUC UUGGGAGCGG CCGUCCCCUG CUCGGCGCCG GCCCGAAGCG UUUAC UUUG AAAAAA(5MU)UAG AGUG(PSU)(PSU)CAAA GCAGGCCGCC UGGAUACCGC AGCUAGGAAU AAUGGAAUAG GACC GCGGU UCUAUUUUGU UGGUUUUCGG AACUGAGGCC AUGAUUAAGA GGGACGGCCG GGGGCAUUCG UAUUGCGCCG CUAGA GGUG AAAUUCUUGG ACCGGCGCAA GACGGACCAG AGCGAAAGCA UUUGCCAAGA AUGUUUUCAU UAAUCAAGA(A2M) CG AAAGUCG GAGGUUCGAA GACGAUCAGA UACCGUCGUA GUUCCGACCA (PSU)AAACGAUGC CGACUGGCGA UGCGGCGGC GUUAUUCCCA UGACCCGCCG GGCAGCUUCC GGGAAACCAA AGUCUUUGGG UUCCGGGGGG AGUAUGGUUG CAAAGCUGAA ACUUAAAGG AAUUGACGGA AGGGCACCAC (B8Q)AGGAGUGGA GCCUGCGGCU UAAU(PSU)UGAC(B8N) CAACACGGG AAACCUCACC CGGCCCGGAC ACGGACAGGA UUGACAGAUU GAUAGCUCUU UCUCGAUUCC GUGGGUGGUG GUGCAUGGC (4AC)GUUCUUAGU UGGUGGAGCG AUUUGUCUGG UUAAUUC(5MC)GA UAACGAACGA GACUCUGGCA UGCUAACUAG UU ACGCGAC CCCGGUCGGC GUAACUUCUU AGAGGGACAA GUGGCGUUCA GCCACCCGAG AUUGAGCAAU AACAGGUCUG UGA UGCCCU UAGAUGUCCG GGGCUGCACG CGCGCUACAC UGACUGGCUC AGCGUGUGCC UACCCUACGC CGGCAGGCGC GGGU AACCC GUUGAACCCC AUUCGUGAUG GGGAUCGGGG AUUGCAAUUA UUCCCCAUGA ACGAGGAAUU CCCAGUAAGU GCGGG UCAU AAGCUUGCGU UGAUU(A2M)AGUC CCUGCCCUUU GUACACACCG (OMC)CCGUCG(OMC)UA CUACCGAUUG GAUG GUUUA GUGAGGCCCU CGGAUCGGCC CCGCCGGGGU GCCCUGGCGG AGCGCUGAGA AGACGGUCGA ACUUG(M7A)CUAU C UAGAGGAA GUAAAAGUCG (UR3)A(6MZ)CAAGGUU UC(4AC)GUAGGUG (MA6)(MA6)CCUGCGGA AGGAUCAUUA

Macromolecule #4: uL2

• Macromolecule: Name: uL2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 26.998613 KDa

Sequence

String:

GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGRLRG T

Macromolecule #5: uL3

• Macromolecule: Name: uL3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 45.119812 KDa

Sequence

String:

SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD AGKRQLDKDF SSMKKYCQVI R VLAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVSQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LP RKTHRGL RKVACIGAWH PARVAFSVAR AGQKGYHHRT EINKKIYKIG QGYLIKDGKL IKNNASTDYD LSDKSINPLG GFV HYGEVT NDFVMLKGCV VGTKKRVLTL RKSLLVQTKR RALEKIDLKF IDTTSKFGHG RFQTVEEKKA FMGPLKKD

Macromolecule #6: uL4

• Macromolecule: Name: uL4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 41.114434 KDa

Sequence

String:

ACARPLISVY SEKGESSGKN VTLPAVFKAP IRPDIVNFVH TNLRKNNRQP YAVSELAGHQ TSAESWGTGR AVARIPRVRG GGTHRSGQG AFGNMCRGGR MFAPTKTWRR WHRRVNTTQK RYAICSALAA SALPALVMSK GHRIEEVPEL PLVVEDKVEG Y KKTKEAVL LLKKLKAWND IKKVYASQRM RAGKGKMRNR RRIQRRGPCV IYNEDNGIVK AFRNIPGITL LNVTKLNILK LA PGGHVGR FCIWTESAFR KLDDLYGTWR KAASLKSNYN LPMHKMLNTD LSRILKSPEI QRALRAPRKK IHRRVLKKNP LKN LRIMLK LNPYA(MLZ)TMRR NTILRQARNH KLRVERAAAA LAAKSD

Macromolecule #7: uL18

• Macromolecule: Name: uL18 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 34.077391 KDa

Sequence

String:

FVKVVKNKAY FKRYQVKFRR RREGKTDYYA RKRLVIQDKN KYNTPKYRMI VRVTNRDIIC QIAYARIEGD MIVCAAYAHE LPKYGVKVG LTNYAAAYCT GLLLARRLLN RFGMDKIYEG QVEVTGDEYN VESIDGQPGA FTCYLDAGLA RTTTGNKVFG A LKGAVDGG LSIPHSTKRF PGYDSESKEF NAEVHRKHIM GQNVADYMRY LMEEDEDAYK KQFSQYIKNN VTPDMMEEMY KK AHAAIRE NPVYEKKPKR EVKKKRWNRP KMSLAQKKDR VAQKKASFLR AQERAA

Macromolecule #8: eL6

• Macromolecule: Name: eL6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 33.028336 KDa

Sequence

String:

MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

Macromolecule #9: uL30

• Macromolecule: Name: uL30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 26.658754 KDa

Sequence

String:

NFAELKIKRL RKKFAQKMLR KARRKLIYEK AKHYHKEYRQ MYRTEIRMAR MARKAGNFYV PAEPKLAFVI RIRGINGVSP KVRKVLQLL RLRQIFNGTF VKLNKASINM LRIVEPYIAW GYPNLKSVNE LIYKRGYGKI NKKRIALTDN TLIARSLGKY N IICMEDLI HEIYTVGKHF KEANNFLWPF KLSSPRGGMK KKTTHFVEGG DAGNREDQIN RLIRRMN

Macromolecule #10: eL8

• Macromolecule: Name: eL8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 36.221516 KDa

Sequence

String:

MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ARAEKKAAGK GDVPTKRPPV LRAGVNTVTT LVENKKAQLV VIAHDVDPIE LVVFLPALCR KMGVPYCILK GK ARLGRLV HRKTCTTVAF TQVNSEDKGA LAKLVEAIRT NYNDRYDEIR RHWGGNVLGP KSVARIAKLE KAKAKELATK LG

Macromolecule #11: uL6

• Macromolecule: Name: uL6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.627215 KDa

Sequence

String:

MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QA

Macromolecule #12: uL16

• Macromolecule: Name: uL16 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.643057 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

Macromolecule #13: uL5

• Macromolecule: Name: uL5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 19.399498 KDa

Sequence

String:

KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKGL KVREYELRK NNFSDTGNFG FGIQEHIDLG IKYDPSIGIY GLDFYVVLGR PGFSIADKKR RTGCIGAKHR ISKEEAMRWF Q QKYDGIIL PG

Macromolecule #14: eL13

• Macromolecule: Name: eL13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.200525 KDa

Sequence

String:

APSRNGMILK PHFHKDWQRR VATWFNQPAR KIRRRKARQA RARRIAPRPA AGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLVLFPRK PSAPKKGDSS AEELKLATQL TGPVMPIRNV F KKEKARVI TEEEKNFKAF ASLRMARANA RLFGIRAKRA KEAAEQDVEK KK

Macromolecule #15: eL14

• Macromolecule: Name: eL14 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.217355 KDa

Sequence

String:

VFRRFVEVGR VAYVSFGPHA GKLVAIVDVI DQNRALVDGP CTRVRRQAMP FKCMQLTDFI LKFPHSARQK YVRKAWEKAD INTKWAATR WAKKIEARER KAKMTDFDRY KVMKAKKMRN RIIKNEVKKL QRAALLKAS

Macromolecule #16: eL15

• Macromolecule: Name: eL15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.076088 KDa

Sequence

String:

GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR KSRGLGKGHK FHHTIGGSRR AAWRRRNTLQ LHRYR

Macromolecule #17: uL13

• Macromolecule: Name: uL13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 23.144859 KDa

Sequence

String:

QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK QLMRLRKQAE KNVEKKISKF TDVLKTHGLL V

Macromolecule #18: uL22

• Macromolecule: Name: uL22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.758678 KDa

Sequence

String:

VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLKKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

Macromolecule #19: eL18

• Macromolecule: Name: eL18 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.568492 KDa

Sequence

String:

GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGRENKTAV VVGTVTDDV RVQEVPKLKV CALRVTSRAR SRILKAGGKI LTFDQLALDS PKGRGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN

Macromolecule #20: eL19

• Macromolecule: Name: eL19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bovine (cattle)
• Molecular weight: Theoretical: 21.613033 KDa

Sequence

String:

SMLRLQKRLA SSVLRCGKKK VWLDPNETNE IANANSRQQI RKLIKDGLII RKPVTVHSRA RCRKNTLARR KGRHMGIGKR KGTANARMP EKVTWMRRMR ILRRLLRRYR ESKKIDRHMY HSLYLKVKGN VFKNKRILME HIHKLKADKA RKKLLADQAE A RRSKTKEA RKRREERLQA KK

Macromolecule #21: eL20

• Macromolecule: Name: eL20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 20.827561 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCRTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

Macromolecule #22: eL21

• Macromolecule: Name: eL21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 18.478791 KDa

Sequence

String:

TNTKGKRRGT RYMFSRPFRK HGVVPLATYM RIYKKGDIVD IKGMGTVQKG MPHKCYHGKT GRVYNVTQHA VGIVVNKQVK GKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR QPAPPREAHF VRTNGKEPEL LEPIPYEFMA

Macromolecule #23: eL22

• Macromolecule: Name: eL22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.495275 KDa

Sequence

String:

QLLKFTLDCT HPVEDGIMDA ANFEQFLQER IKVNGKAGNL GGGVVSLERS KSKITVTSEV PFSKRYLKYL TKKYLKKNNL RDWLRVVAN TKESYELRYF

Macromolecule #24: uL14

• Macromolecule: Name: uL14 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 13.972425 KDa

Sequence

String:

SGAKFRISLG LPVGAVINCA DNTGAKNLYI ISVKGIKGRL NRLPAAGVGD MVMATVKKGK PELRKKVHPA VVIRQRKSYR RKDGVFLYF EDNAGVIVNN KGEMKGSAIT GPVAKECADL WPRIASNAGS IA

Macromolecule #25: eL24

• Macromolecule: Name: eL24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

Macromolecule #26: uL23

• Macromolecule: Name: uL23 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 13.727181 KDa

Sequence

String:

KIRTSPTFRR PKTLRLRRQP KYPRKSAPRR NKLDHYAIIK FPLTTESAMK KIEDNNTLVF IVDVKANKHQ IKQAVKKLYD IDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII

Macromolecule #27: uL24

• Macromolecule: Name: uL24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.891787 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGK

Macromolecule #28: eL27

• Macromolecule: Name: eL27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.704635 KDa

Sequence

String:

GKFMKPGKVV LVLAGRYSGR KAVIVKNIDD GTSDRPYSHA LVAGIDRYPR KVTAAMGKKK IAKRSKIKSF VKVYNYNHLM PTRYSVDIP LDKTVVNKDV FRDPALKRKA RREAKVKFEE RYKTGKNKWF FQKLRF

Macromolecule #29: uL15

• Macromolecule: Name: uL15 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.489363 KDa

Sequence

String:

PSRLRKTRKL RGHVSHGHGR IGKHRKHPGG RGNAGGMHHH RINFDKYHPG YFGKVGMRHY HLKRNQSFCP TVNLDKLWTL VSEQTRVNA AKNKTGAAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKGV GGACVLVA

Macromolecule #30: eL29

• Macromolecule: Name: eL29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 26.708707 KDa

Sequence

String:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPKGA QP PAKAP

Macromolecule #31: eL30

• Macromolecule: Name: eL30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 10.914756 KDa

Sequence

String:

SLESINSRLQ LVMKSGKYVL GYKQSLKMIR QGKAKLVILA NNCPALRKSE IEYYAMLAKT GVHHYSGNNI ELGTACGKYY RVCTLSIID PGDSDIIRS

Macromolecule #32: eL31

• Macromolecule: Name: eL31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.635694 KDa

Sequence

String:

NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLV TYVPVTTFKN LQTVNVDE

Macromolecule #33: eL32

• Macromolecule: Name: eL32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.022021 KDa

Sequence

String:

AALRPLVKPK IVKKRTKKFI RHQSDRYVKI KRNWRKPRGI DNRVRRRFKG QILMPNIGYG SNKKTKHMLP SGFRKFLVHN VKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARL

Macromolecule #34: eL33

• Macromolecule: Name: eL33 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.449612 KDa

Sequence

String:

SGRLWCKAIF AGYKRGLRNQ REHTALLKIE GVYARDETEF YLGKRCAYVY KAKNNTVTPG GKPNKTRVIW GKVTRAHGNS GMVRAKFRS NLPAKAIGHR IRVMLYPSRI

Macromolecule #35: eL34

• Macromolecule: Name: eL34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.994621 KDa

Sequence

String:

VQRLTYRRRL SYNTASNKTR LSRTPGNRIV YLYTKKVGKA PKSACGVCPG RLRGVRAVRP KVLMRLSKTK KHVSRAYGGS MCAKCVRDR IKRAFLIEEQ KIVVKVLKAQ AQSQK

Macromolecule #36: uL29

• Macromolecule: Name: uL29 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.435403 KDa

Sequence

String:

AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLNKHEE SLKTKKQQRK ERLYPLRKYA VKA

Macromolecule #37: eL36

• Macromolecule: Name: eL36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.888371 KDa

Sequence

String:

ALRYPMAVGL NKGHKVTKNV SKPRHSRRRG RLTKHTKFVR DMIREVCGFA PYERRAMELL KVSKDKRALK FIKKRVGTHI RAKRKREEL SSVLAAMRKA AAK

Macromolecule #38: eL37

• Macromolecule: Name: eL37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 10.090891 KDa

Sequence

String:

TKGTSSFGKR RNKTHTLCRR CGSKAYHLQK STCGKCGYPA KRKRKYNWSA KAKRRNTTGT GRMRHLKIVY RRFRHGFREG TTPKPK

Macromolecule #39: eL38

• Macromolecule: Name: eL38 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 8.107752 KDa

Sequence

String:

PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKELK

Macromolecule #40: eL39

• Macromolecule: Name: eL39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.324579 KDa

Sequence

String:

SSHKTFRIKR FLAKKQKQNR PIPQWIWMKT GNKIRYNSKR RHWRRTKLGL

Macromolecule #41: eL40

• Macromolecule: Name: eL40 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.212593 KDa

Sequence

String:

IIEPSLRQLA QKYNCDKMIC R(MLZ)CYARLHPR AVNCRKKKCG HTNNLRPKKK VK

Macromolecule #42: eL41

• Macromolecule: Name: eL41 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

Macromolecule #43: eL42

• Macromolecule: Name: eL42 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 12.070474 KDa

Sequence

String:

VNVPKTRRTF CKKCGKHQPH KVTQYKKGKD SLYAQGKRRY DRKQSGYGGQ TKPIFRKKAK TTKKIVLRLE CVEPNCRSKR MLAIKRCKH FELGGDKKRK GQVI

Macromolecule #44: eL43

• Macromolecule: Name: eL43 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 10.168153 KDa

Sequence

String:

AKRTKKVGIV GKYGTRYGAS LRKMVKKIEI SQHAKYTCSF CGKTKMKRRA VGIWHCGSCM KTVAGGAWTY NTTSAVTVKS AIRRLKELK DQ

Macromolecule #45: eL28

• Macromolecule: Name: eL28 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.220686 KDa

Sequence

String:

SAHLQWMVVR NCSSFLIKRN KQTYSTEPNN LKARNSFRYN GLIHRKTVGV EPAADGKGVV VVMKRRSGQR KPATSYVRTT INKNARATL SSIRHMIRKN KYHPDLRMAA IRRASAILRS QKPVM

Macromolecule #46: uL10

• Macromolecule: Name: uL10 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.521062 KDa

Sequence

String:

DRATWKSNYF LKIIQLLDDY PKCFIVGADN VGSKQMQQIR MSLRGKAVVL MGKNTMMRKA IRGHLENNPA LEKLLPHIRG NVGFVFTKE DLTEIRDMLL ANKVPAAARA GAIAPCEVTV PAQNTGLGPE KTSFFQALGI TTKISRGTIE ILSDVQLIKT G DKVGASEA TLLNMLNISP FSFGLIIQQV FDNGSIYN

Macromolecule #47: uL11

• Macromolecule: Name: uL11 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.561117 KDa

Sequence

String:

EIKVVYLRCT GGEVGATSAL APKIGPLGLS PKKVGDDIAK ATGDWKGLRI TVKLTIQNRQ AQIEVVPSAS ALIIKALKEP PRDRKKQKN IKHSGNITFD EIVNIARQMR HRSLARELSG TIKEILGTAQ SVGCNVDGRH PHDIIDDINS GAVE

Macromolecule #48: eEF2

• Macromolecule: Name: eEF2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 94.587258 KDa

Sequence

String:

FTVDQIRAIM DKKANIRNMS VIAHVDHGKS TLTDSLVCKA GIIARFTDTR KDEQERCITI KSTAISLFYE LSENDLNFIK QSKDGAGFL INLIDSPGHV DFSSEVTAAL RVTDGALVVV DCVSGVCVQT ETVLRQAIAE RIKPVLMMNK MDRALLELQL E PEELYQTF QRIVENVNVI ISTYGEGESG PMGNIMIDPV LGTVGFGSGL HGWAFTLKQF AEMYVAKFAA KGEGQLGPAE RA KKVEDMM KKLWGDRYFD PANGKFSKSA TSPEGKKLPR TFCQLILDPI FKVFDAIMNF KKEETAKLIE KLDIKLDSED KDK EGKPLL KAVMRRWLPA GDALLQMITI HLPSPVTAQK YRCELLYEGP PDDEAAMGIK SCDPKGPLMM YISKMVPTSD KGRF YAFGR VFSGLVSTGL KVRIMGPNYT PGKKEDLYLK PIQRTILMMG RYVEPIEDVP CGNIVGLVGV DQFLVKTGTI TTFEH AHNM RVMKFSVSPV VRVAVEAKNP ADLPKLVEGL KRLAKSDPMV QCIIEESGEH IIAGAGELHL EICLKDLEED HACIPI KKS DPVVSYRETV SEESNVLCLS KSPNKHNRLY MKARPFPDGL AEDIDKGEVS ARQELKQRAR YLAEKYEWDV AEARKIW CF GPDGTGPNIL TDITKGVQYL NEIKDSVVAG FQWATKEGAL CEENMRGVRF DVHDVTLHAD AI(DDE)RGGGQII PTAR RCLYA SVLTAQPRLM EPIYLVEIQC PEQVVGGIYG VLNRKRGHVF EESQVAGTPM FVVKAYLPVN ESFGFTADLR SNTGG QAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL

Macromolecule #49: SERBP1

• Macromolecule: Name: SERBP1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.25769 KDa

Sequence

String:

DGFDSRGKRE FDRHSGSDRS GLKHEDKRGG SGSHNWGTVK DELTLDEWKA IQNKD

Macromolecule #51: uS2

• Macromolecule: Name: uS2 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.361861 KDa

Sequence

String:

SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQTAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPEE IEKEEQAAA

Macromolecule #52: eS1

• Macromolecule: Name: eS1 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.759145 KDa

Sequence

String:

VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF HGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ QVRQIRKKMM EIMTREVQTN D LKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHG

Macromolecule #53: uS5

• Macromolecule: Name: uS5 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.441846 KDa

Sequence

String:

KEWLPVTKLG RLVKDMKIKS LEEIYLFSLP IKESEIIDFF LGASLKDEVL KIMPVQKQTR AGQRTRFKAF VAIGDYNGHV GLGVKCSKE VATAIRGAII LAKLSIVPVR RGYWGNKIGK PHTVPCKVTG RCGSVLVRLI PAPRGTGIVS APVPKKLLMM A GIDDCYTS ARGCTATLGN FAKATFDAIS KTYSYLTPDL WKETVFTKSP YQEFTDHLVK THT

Macromolecule #54: uS3

• Macromolecule: Name: uS3 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 25.215586 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG

Macromolecule #55: eS4

• Macromolecule: Name: eS4 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 29.523674 KDa

Sequence

String:

ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNR LKYALTGDEV KKICMQRFIK IDGKVRTDIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI Q IDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RG KGIRLTI AEERDKRLAA KQSSG

Macromolecule #56: uS7

• Macromolecule: Name: uS7 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

Macromolecule #57: eS6

• Macromolecule: Name: eS6 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 27.471535 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSL

Macromolecule #58: eS7

• Macromolecule: Name: eS7 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

Macromolecule #59: eS8

• Macromolecule: Name: eS8 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 24.003012 KDa

Sequence

String:

GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKG

Macromolecule #60: uS4

• Macromolecule: Name: uS4 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 21.649633 KDa

Sequence

String:

PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK KGQGGAG

Macromolecule #61: eS10

• Macromolecule: Name: eS10 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.529682 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRR

Macromolecule #62: uS17

• Macromolecule: Name: uS17 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

Macromolecule #63: eS12

• Macromolecule: Name: eS12 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 13.048244 KDa

Sequence

String:

VNTALQEVLK TALIHDGLAR GIREAAKALD KRQAHLCVLA SNCDEPMYVK LVEALCAEHQ INLIKVDDNK KLGEWVGLCK IDREGKPRK VVGCSCVVVK DYGKESQAKD VIEEYFKC

Macromolecule #64: uS15

• Macromolecule: Name: uS15 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 17.057113 KDa

Sequence

String:

GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV

Macromolecule #65: uS11

• Macromolecule: Name: uS11 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.544659 KDa

Sequence

String:

SLGPQVAEGE NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQRC KELGITALHI KLRATGGNR TKTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRRL

Macromolecule #66: uS19

• Macromolecule: Name: uS19 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.646297 KDa

Sequence

String:

FRKFTYRGVD LDQLLDMSYE QLMQLYSARQ RRRLSRGLRR KQHSLLKRLR KAKKEAPPME KPEVVKTHLR DMIILPEMVG SMVGVYNGK TFNQVEIKPE MIGHYLGEFS ITYKPVKHGR PGIGAT

Macromolecule #67: uS9

• Macromolecule: Name: uS9 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.032804 KDa

Sequence

String:

GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERFA GVDIRVRVKG GGHVAQIYAI RQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG GPGARARYQK SYR

Macromolecule #68: eS17

• Macromolecule: Name: eS17 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.250714 KDa

Sequence

String:

GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGMNFKT PRG

Macromolecule #69: uS13

• Macromolecule: Name: uS13 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 16.898648 KDa

Sequence

String:

SLVIPEKFQH ILRVLNTNID GRRKIAFAIT AIKGVGRRYA HVVLRKADID LTKRAGELTE DEVERVITIM QNPRQYKIPD WFLNRQKDV KDGKYSQVLA NGLDNKLRED LERLKKIRAH RGLRHFWGLR VRGQHTKTTG RRGRT

Macromolecule #70: eS19

• Macromolecule: Name: eS19 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.611003 KDa

Sequence

String:

GVTVKDVNQQ EFVRALAAFL KKSGKLKVPE WVDTVKLAKH KELAPYDENW FYTRAASTAR HLYLRGGAGV GSMTKIYGGR QRNGVMPSH FSRGSKSVAR RVLQALEGLK MVEKDQDGGR KLTPQGQRDL DRIAGQVAAA KK

Macromolecule #71: uS10

• Macromolecule: Name: uS10 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.395489 KDa

Sequence

String:

HRIRITLTSR NVKSLEKVCA DLIRGAKEKN LKVKGPVRMP TKTLRITTRK TPCGEGSKTW DRFQMRIHKR LIDLHSPSEI VKQITSISI EPGVEVEVTI A

Macromolecule #72: eS21

• Macromolecule: Name: eS21 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

Macromolecule #73: uS8

• Macromolecule: Name: uS8 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.734357 KDa

Sequence

String:

VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

Macromolecule #74: uS12

• Macromolecule: Name: uS12 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 15.626392 KDa

Sequence

String:

GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

Macromolecule #75: eS24

• Macromolecule: Name: eS24 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 14.432125 KDa

Sequence

String:

TVTIRTRKFM TNRLLQRKQM VIDVLHPGKA TVPKTEIREK LAKMYKTTPD VIFVFGFRTH FGGGKTTGFG MIYDSLDYAK KNEPKHRLA RHGLYEKKKT SRKQRKERKN RMKKVRGTAK ANVGA

Macromolecule #76: eS25

• Macromolecule: Name: eS25 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 8.526119 KDa

Sequence

String:

RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKG

Macromolecule #77: eS26

• Macromolecule: Name: eS26 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 11.645794 KDa

Sequence

String:

TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR FR

Macromolecule #78: eS27

• Macromolecule: Name: eS27 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 9.34899 KDa

Sequence

String:

PLAKDLLHPS PEEEKRKHKK KRLVQSPNSY FMDVKCPGCY KITTVFSHAQ TVVLCVGCST VLCQPTGGKA RLTEGCSFRR KQH

Macromolecule #79: eS28

• Macromolecule: Name: eS28 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 7.007069 KDa

Sequence

String:

QPIKLARVTK VLGRTGSQGQ CTQVRVEFMD DTSRSIIRNV KGPVREGDVL TLLESEREAR RL

Macromolecule #80: uS14

• Macromolecule: Name: uS14 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.559625 KDa

Sequence

String:

GHQQLYWSHP RKFGQGSRSC RVCSNRHGLI RKYGLNMCRQ CFRQYAKDIG FIKLD

Macromolecule #81: eS30

• Macromolecule: Name: eS30 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 6.317539 KDa

Sequence

String:

SLARVGKVRG QTLKVAKQEK KKKRTGRAKR RMQYNRRFVN VVPTFGKKKG PNANS

Macromolecule #82: eS31

• Macromolecule: Name: eS31 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 7.98644 KDa

Sequence

String:

KSYTTPKKNK HKRKKVKLAV LKYYKVDENG KISRLRRECP SDECGAGVFM ASHFDRHYCG KCCLTYCF

Macromolecule #83: RACK1

• Macromolecule: Name: RACK1 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Rabbit (rabbit)
• Molecular weight: Theoretical: 34.669113 KDa

Sequence

String:

TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTI

Macromolecule #84: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 84 / Number of copies: 298 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #85: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 85 / Number of copies: 8 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #86: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 86 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 5.0 nm
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Frames/image: 1-17 / Average exposure time: 1.1 sec. / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Calibrated magnification: 104478 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: Gctf

Startup model

Type of model: EMDB MAP
EMDB ID:

4129

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 133480
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.0)

Atomic model buiding 1

Initial model

PDB ID:

5lzv

• Refinement: Space: REAL / Protocol: OTHER

Output model

PDB-6mte:
Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)