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- EMDB-10321: Mus musculus brain neocortex ribosome 60S bound to Ebp1 -

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Basic information

Entry
Database: EMDB / ID: EMD-10321
TitleMus musculus brain neocortex ribosome 60S bound to Ebp1
Map dataex vivo derived mus musculus P0 brain neocortex ribosome containing eEF2 and EBP1
Sample
  • Complex: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound to Ebp1
    • Protein or peptide: x 43 types
    • RNA: x 3 types
  • Ligand: x 2 types
Function / homology
Function and homology information


5.8S rRNA binding / Protein hydroxylation / translation at postsynapse / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit ...5.8S rRNA binding / Protein hydroxylation / translation at postsynapse / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / selenocysteine insertion sequence binding / aminoacyl-tRNA synthetase multienzyme complex / embryonic brain development / eukaryotic 80S initiation complex / negative regulation of protein neddylation / translation at presynapse / axial mesoderm development / regulation of G1 to G0 transition / negative regulation of formation of translation preinitiation complex / exit from mitosis / protein-DNA complex disassembly / 90S preribosome assembly / optic nerve development / TORC2 complex binding / GAIT complex / G1 to G0 transition / retinal ganglion cell axon guidance / middle ear morphogenesis / peroxisome proliferator activated receptor binding / A band / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / alpha-beta T cell differentiation / response to aldosterone / positive regulation of axonogenesis / homeostatic process / lung morphogenesis / macrophage chemotaxis / cell-substrate adhesion / smooth endoplasmic reticulum / growth factor binding / protein-RNA complex assembly / blastocyst development / translation regulator activity / protein localization to nucleus / cellular response to actinomycin D / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / protein targeting / positive regulation of G1/S transition of mitotic cell cycle / positive regulation of axon extension / MDM2/MDM4 family protein binding / ribonucleoprotein complex binding / rough endoplasmic reticulum / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal large subunit biogenesis / cellular response to interleukin-4 / Neutrophil degranulation / ossification / cytosolic ribosome / : / positive regulation of translation / regulation of signal transduction by p53 class mediator / innate immune response in mucosa / mRNA 3'-UTR binding / skeletal system development / positive regulation of cell differentiation / sensory perception of sound / multicellular organism growth / mRNA 5'-UTR binding / bone development / transcription coactivator binding / cytoplasmic ribonucleoprotein granule / rRNA processing / cellular response to type II interferon / ribosomal large subunit assembly / transcription corepressor activity / large ribosomal subunit rRNA binding / antimicrobial humoral immune response mediated by antimicrobial peptide / large ribosomal subunit / presynapse / regulation of translation / retina development in camera-type eye / heparin binding / cell body / 5S rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / antibacterial humoral response / fibroblast proliferation / postsynapse / killing of cells of another organism / defense response to Gram-negative bacterium / tRNA binding / negative regulation of translation / response to lipopolysaccharide / postsynaptic density / nucleic acid binding / rRNA binding
Similarity search - Function
PA2G4 family / : / Peptidase M24A, methionine aminopeptidase, subfamily 2, binding site / Methionine aminopeptidase subfamily 2 signature. / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal ...PA2G4 family / : / Peptidase M24A, methionine aminopeptidase, subfamily 2, binding site / Methionine aminopeptidase subfamily 2 signature. / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Peptidase M24 / Metallopeptidase family M24 / Ribosomal L28e protein family / Creatinase/aminopeptidase-like / : / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal protein L10e, conserved site / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal L29e protein family / Ribosomal protein L24e, conserved site / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 60S ribosomal protein L35 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal L27e protein family / Ribosomal Protein L6, KOW domain / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L31e, conserved site / Ribosomal protein L37ae / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal L40e family / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L7A/L8 / Ribosomal protein L27e signature. / Ribosomal protein L10e signature. / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L30, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal L30 N-terminal domain / Ribosomal protein L19/L19e conserved site / Ribosomal L37ae protein family / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19e signature. / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L6e signature. / Ribosomal_L31e / Ribosomal protein L15e, conserved site / Ribosomal protein L30e signature 1. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L14e domain
Similarity search - Domain/homology
Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL6 ...Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein eL36 / Proliferation-associated protein 2G4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein eS32 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein eL19 / Ribosomal protein L26 / Ribosomal protein L37a / Ribosomal protein L39 / 60S ribosomal protein L27 / 60S ribosomal protein L40 / Ribosomal protein L36A / 60S ribosomal protein L10 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein eL38
Similarity search - Component
Biological speciesMus musculus (house mouse) / Mouse (mice)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsKraushar ML / Sprink T
Funding support Germany, 4 items
OrganizationGrant numberCountry
European Molecular Biology Organization (EMBO)190-2016 Germany
European Union (EU)iNEXT (PID: 2227) Germany
Alexander von Humboldt Foundation Germany
European Union (EU)Instruct-ERIC Pilot R&D Project (APPID: 232) Germany
CitationJournal: Mol Cell / Year: 2021
Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.
Authors: Matthew L Kraushar / Ferdinand Krupp / Dermot Harnett / Paul Turko / Mateusz C Ambrozkiewicz / Thiemo Sprink / Koshi Imami / Manuel Günnigmann / Ulrike Zinnall / Carlos H Vieira-Vieira / ...Authors: Matthew L Kraushar / Ferdinand Krupp / Dermot Harnett / Paul Turko / Mateusz C Ambrozkiewicz / Thiemo Sprink / Koshi Imami / Manuel Günnigmann / Ulrike Zinnall / Carlos H Vieira-Vieira / Theres Schaub / Agnieszka Münster-Wandowski / Jörg Bürger / Ekaterina Borisova / Hiroshi Yamamoto / Mladen-Roko Rasin / Uwe Ohler / Dieter Beule / Thorsten Mielke / Victor Tarabykin / Markus Landthaler / Günter Kramer / Imre Vida / Matthias Selbach / Christian M T Spahn /
Abstract: Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during ...Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit (TE) factor during protein synthesis at near-atomic resolution by cryoelectron microscopy (cryo-EM). Ribosome profiling demonstrated Ebp1-60S binding is highest during start codon initiation and N-terminal peptide elongation, regulating ribosome occupancy of these codons. Membrane-targeting domains emerging from the 60S tunnel, which recruit SRP/Sec61 to the shared binding site, displace Ebp1. Ebp1 is particularly abundant in the early-born neural stem cell (NSC) lineage and regulates neuronal morphology. Ebp1 especially impacts the synthesis of membrane-targeted cell adhesion molecules (CAMs), measured by pulsed stable isotope labeling by amino acids in cell culture (pSILAC)/bioorthogonal noncanonical amino acid tagging (BONCAT) mass spectrometry (MS). Therefore, Ebp1 is a central component of protein synthesis, and the ribosome TE is a focal point of gene expression control in the molecular specification of neuronal morphology during development.
History
DepositionSep 20, 2019-
Header (metadata) releaseSep 30, 2020-
Map releaseSep 30, 2020-
UpdateFeb 17, 2021-
Current statusFeb 17, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 4
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6swa
  • Surface level: 4
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10321.png

Downloads & links

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Map

FileDownload / File: emd_10321.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationex vivo derived mus musculus P0 brain neocortex ribosome containing eEF2 and EBP1
Voxel sizeX=Y=Z: 1.3267 Å
Density
Contour LevelBy AUTHOR: 4.0 / Movie #1: 4
Minimum - Maximum-10.475252 - 20.985825
Average (Standard dev.)0.0008521894 (±0.8978186)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 477.612 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.32671.32671.3267
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z477.612477.612477.612
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-10.76222.0310.001

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Supplemental data

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Supplemental map: emd 10321 additional 1.map

Fileemd_10321_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mus musculus postnatal day 0 brain neocortex 80S ribosome bound t...

EntireName: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound to Ebp1
Components
  • Complex: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound to Ebp1
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L14
    • Protein or peptide: 60S ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L13a
    • Protein or peptide: 60S ribosomal protein L17
    • Protein or peptide: 60S ribosomal protein L18
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60S ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L23a
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: 60S ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: Ribosomal protein L39
    • Protein or peptide: 60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: Ribosomal protein L36ARibosome
    • Protein or peptide: Ribosomal protein L37aRibosome
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L28
    • RNA: 28S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: Proliferation-associated protein 2G4
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound t...

SupramoleculeName: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound to Ebp1
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#46
Source (natural)Organism: Mus musculus (house mouse) / Strain: CD1 / Organ: brain / Tissue: neocortex / Location in cell: cytoplasm
Molecular weightTheoretical: 3.2 MDa

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Macromolecule #1: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 27.456158 KDa
SequenceString: GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV ...String:
GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGRLRG TKTVQ

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Macromolecule #2: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 45.22398 KDa
SequenceString: SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDASKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD TGKKQLEKDF NSMKKYCQVI R IIAHTQMR ...String:
SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDASKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD TGKKQLEKDF NSMKKYCQVI R IIAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVNQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LP RKTHRGL RKVACIGAWH PARVAFSVAR AGQKGYHHRT EINKKIYKIG QGYLIKDGKL IKNNASTDYD LSDKSINPLG GFV HYGEVT NDFIMLKGCV VGTKKRVLTL RKSLLVQTKR RALEKIDLKF IDTTSKFGHG RFQTMEEKKA FMGPLKKD

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Macromolecule #3: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 41.321762 KDa
SequenceString: CARPLISVYS EKGESSGKNV TLPAVFKAPI RPDIVNFVHT NLRKNNRQPY AVSELAGHQT SAESWGTGRA VARIPRVRGG GTHRSGQGA FGNMCRGGRM FAPTKTWRRW HRRVNTTQKR YAICSALAAS ALPALVMSKG HRIEEVPELP LVVEDKVEGY K KTKEAVQL ...String:
CARPLISVYS EKGESSGKNV TLPAVFKAPI RPDIVNFVHT NLRKNNRQPY AVSELAGHQT SAESWGTGRA VARIPRVRGG GTHRSGQGA FGNMCRGGRM FAPTKTWRRW HRRVNTTQKR YAICSALAAS ALPALVMSKG HRIEEVPELP LVVEDKVEGY K KTKEAVQL LKKLKAWNDI KKVYASQRMR AGKGKMRNRR RIQRRGPCII YNEDNGIIKA FRNIPGITLL NVSKLNILKL AP GGHVGRF CIWTESAFRK LDELYGTWRK AASLKSNYNL PMHKMMNTDL SRILKSPEIQ RALRAPRKKI HRRVLKKNPL KNL RIMLKL NPYAKTMRRN TILRQARNHK LRVKKLEAAA TALATKS

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Macromolecule #4: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 34.129383 KDa
SequenceString: VKVVKNKAYF KRYQVRFRRR REGKTDYYAR KRLVIQDKNK YNTPKYRMIV RVTNRDIICQ IAYARIEGDM IVCAAYAHEL PKYGVKVGL TNYAAAYCTG LLLARRLLNR FGMDKIYEGQ VEVNGGEYNV ESIDGQPGAF TCYLDAGLAR TTTGNKVFGA L KGAVDGGL ...String:
VKVVKNKAYF KRYQVRFRRR REGKTDYYAR KRLVIQDKNK YNTPKYRMIV RVTNRDIICQ IAYARIEGDM IVCAAYAHEL PKYGVKVGL TNYAAAYCTG LLLARRLLNR FGMDKIYEGQ VEVNGGEYNV ESIDGQPGAF TCYLDAGLAR TTTGNKVFGA L KGAVDGGL SIPHSTKRFP GYDSESKEFN AEVHRKHIMG QNVADYMRYL MEEDEDAYKK QFSQYIKNNV TPDMMEEMYK KA HAAIREN PVYEKKPKRE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES

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Macromolecule #5: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 22.35933 KDa
SequenceString: TVGGDKNGGT RVVKLRKMPR YYPTEDVPRK LLSHGKKPFS QHVRRLRSSI TPGTVLIILT GRHRGKRVVF LKQLDSGLLL VTGPLVINR VPLRRTHQKF VIATSTKVDI SDVKIPKHLT DAYFKKKQLR KPRHQEGEIF DTEKEKYEIT EQRKADQKAV D LQILPKIK ...String:
TVGGDKNGGT RVVKLRKMPR YYPTEDVPRK LLSHGKKPFS QHVRRLRSSI TPGTVLIILT GRHRGKRVVF LKQLDSGLLL VTGPLVINR VPLRRTHQKF VIATSTKVDI SDVKIPKHLT DAYFKKKQLR KPRHQEGEIF DTEKEKYEIT EQRKADQKAV D LQILPKIK AVPQLQGYLR SQFSLTNGMY PHKLVF

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Macromolecule #6: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 27.674057 KDa
SequenceString: PETLKKKRRN FAELKVKRLR KKFALKTLRK ARRKLIYEKA KHYHKEYRQM YRTEIRMARM ARKAGNFYVP AEPKLAFVIR IRGINGVSP KVRKVLQLLR LRQIFNGTFV KLNKASINML RIVEPYIAWG YPNLKSVNEL IYKRGYGKIN KKRIALTDNS L IARSLGKF ...String:
PETLKKKRRN FAELKVKRLR KKFALKTLRK ARRKLIYEKA KHYHKEYRQM YRTEIRMARM ARKAGNFYVP AEPKLAFVIR IRGINGVSP KVRKVLQLLR LRQIFNGTFV KLNKASINML RIVEPYIAWG YPNLKSVNEL IYKRGYGKIN KKRIALTDNS L IARSLGKF GIICMEDLIH EIYTVGKRFK EANNFLWPFK LSSPRGGMKK KTTHFVEGGD AGNREDQINR LIRRMN

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Macromolecule #7: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 26.705521 KDa
SequenceString: NPLFEKRPKN FGIGQDIQPK RDLTRFVKWP RYIRLQRQRA ILYKRLKVPP AINQFTQALD RQTATQLLKL AHKYRPETKQ EKKQRLLAR AEKKAAGKGD VPTKRPPVLR AGVNTVTTLV ENKKAQLVVI AHDVDPIELV VFLPALCRKM GVPYCIIKGK A RLGHLVHR ...String:
NPLFEKRPKN FGIGQDIQPK RDLTRFVKWP RYIRLQRQRA ILYKRLKVPP AINQFTQALD RQTATQLLKL AHKYRPETKQ EKKQRLLAR AEKKAAGKGD VPTKRPPVLR AGVNTVTTLV ENKKAQLVVI AHDVDPIELV VFLPALCRKM GVPYCIIKGK A RLGHLVHR KTCTTVAFTQ VNSEDKGALA KLVEAIRTNY NDRYDEIRRH WGGNVLGPKS VARIAKLEKA KAKELA

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Macromolecule #8: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 21.788369 KDa
SequenceString:
MKTILSNQTV DIPENVEITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR TGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QAD

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Macromolecule #9: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 24.38176 KDa
SequenceString: RRPARCYRYC KNKPYPKSRF CRGVPDAKIR IFDLGRKKAK VDEFPLCGHM VSDEYEQLSS EALEAARICA NKYMVKSCGK DGFHIRVRL HPFHVIRINK MLSCAGADRL QTGMRGAFGK PQGTVARVHI GQVIMSIRTK LQNKEHVIEA LRRAKFKFPG R QKIHISKK ...String:
RRPARCYRYC KNKPYPKSRF CRGVPDAKIR IFDLGRKKAK VDEFPLCGHM VSDEYEQLSS EALEAARICA NKYMVKSCGK DGFHIRVRL HPFHVIRINK MLSCAGADRL QTGMRGAFGK PQGTVARVHI GQVIMSIRTK LQNKEHVIEA LRRAKFKFPG R QKIHISKK WGFTKFNADE FEDMVAEKRL IPDGCGVKYI PNRGPLDKWR ALH

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Macromolecule #10: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 19.270318 KDa
SequenceString:
ENPMRELRIR KLCLNICVGE SGDRLTRAAK VLEQLTGQTP VFSKARYTVR SFGIRRNEKI AVHCTVRGAK AEEILEKGLK VREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ Q KYDGIILP G

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Macromolecule #11: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 23.617697 KDa
SequenceString: APSRNGMILK PHFHKDWQQR VDTWFNQPAR KIRRRKARQA KARRIAPRPA SGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLILFPRK PSAPKKGDSS AEELKLATQL TGPVMPIRNV Y KKEKARVI ...String:
APSRNGMILK PHFHKDWQQR VDTWFNQPAR KIRRRKARQA KARRIAPRPA SGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLILFPRK PSAPKKGDSS AEELKLATQL TGPVMPIRNV Y KKEKARVI TEEEKNFKAF ASLRMARANA RLFGIRAKRA KEAAEQD

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Macromolecule #12: 60S ribosomal protein L14

MacromoleculeName: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 16.301475 KDa
SequenceString:
VFRRYVEVGR VAYISFGPHA GKLVAIVDVI DQNRALVDGP CTRVRRQAMP FKCMQLTDFI LKFPHSARQK YVRKAWEKAD INTKWAATR WAKKIDARER KAKMTDFDRF KVMKAKKMRN RIIKTEVKKL QRAAILKASP

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Macromolecule #13: 60S ribosomal protein L15

MacromoleculeName: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 24.076088 KDa
SequenceString: GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR ...String:
GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR KSRGLGKGHK FHHTIGGSRR AAWRRRNTLQ LHRYR

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Macromolecule #14: 60S ribosomal protein L13a

MacromoleculeName: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 22.748414 KDa
SequenceString: QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALERLK VLDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KMHYRKKK ...String:
QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALERLK VLDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KMHYRKKK QILRLRKQAE KNVEKKICKF TEVLKTN

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Macromolecule #15: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 17.758678 KDa
SequenceString:
VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLKKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

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Macromolecule #16: 60S ribosomal protein L18

MacromoleculeName: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 21.411363 KDa
SequenceString:
DIRHNKDRKV RRKEPKSQDI YLRLLVKLYR FLARRTNSTF NQVVLKRLFM SRTNRPPLSL SRMIRKMKLP GRENKTAVVV GTVTDDVRI LEVPKLKVCA LRVSSRARSR ILKAGGKILT FDQLALESPK GRGTVLLSGP RKGREVYRHF GKAPGTPHSH T KPYVRSKG RKFERARGRR ASRGYKN

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Macromolecule #17: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 20.631312 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAAGKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTF

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Macromolecule #18: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 18.274523 KDa
SequenceString:
TNTKGKRRGT RYMFSRPFRK HGVVPLATYM RIYKKGDIVD IKGMGTVQKG MPHKCYHGKT GRVYNVTQHA VGIIVNKQVK GKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR HAAPPREAHF VRTNGKEPEL LEPIPYEF

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Macromolecule #19: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 11.738557 KDa
SequenceString:
KQVLKFTLDC THPVEDGIMD AANFEQFLQE RIKVNGKAGN LGGGVVTIER SKSKITVTSE VPFSKRYLKY LTKKYLKKNN LRDWLRVVA NSKESYELRY FQ

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Macromolecule #20: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 13.828295 KDa
SequenceString:
AKFRISLGLP VGAVINCADN TGAKNLYIIS VKGIKGRLNR LPAAGVGDMV MATVKKGKPE LRKKVHPAVV IRQRKSYRRK DGVFLYFED NAGVIVNNKG EMKGSAITGP VAKECADLWP RIASNAGSIA

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Macromolecule #21: 60S ribosomal protein L24

MacromoleculeName: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 7.381578 KDa
SequenceString:
KVELCSFSGY KIYPGHGRRY ARTDGKVFQF LNAKCESAFL SKRNPRQINW TVLYRRKHKK GQ

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Macromolecule #22: 60S ribosomal protein L23a

MacromoleculeName: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 13.727181 KDa
SequenceString:
KIRTSPTFRR PKTLRLRRQP KYPRKSAPRR NKLDHYAIIK FPLTTESAMK KIEDNNTLVF IVDVKANKHQ IKQAVKKLYD IDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII

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Macromolecule #23: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 15.161899 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQ

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Macromolecule #24: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 15.647584 KDa
SequenceString:
KFMKPGKVVL VLAGRYSGRK AVIVKNIDDG TSDRPYSHAL VAGIDRYPRK VTAAMGKKKI AKRSKIKSFV KVYNYNHLMP TRYSVDIPL DKTVVNKDVF RDPALKRKAR REAKVKFEER YKTGKNKWFF QKLRF

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Macromolecule #25: 60S ribosomal protein L27a

MacromoleculeName: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 16.517418 KDa
SequenceString:
PSRLRKTRKL RGHVSHGHGR IGKHRKHPGG RGNAGGMHHH RINFDKYHPG YFGKVGMRHY HLKRNQSFCP TVNLDKLWTL VSEQTRVNA AKNKTGVAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKGV GGACVLVA

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Macromolecule #26: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 7.964323 KDa
SequenceString:
AKSKNHTTHN QSRKWHRNGI KKPRSQRYES LKGVDPKFLR NMRFAKKHNK KGLKKMQANN AKAVSARA

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Macromolecule #27: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 10.811743 KDa
SequenceString:
SINSRLQLVM KSGKYVLGYK QTLKMIRQGK AKLVILANNC PALRKSEIEY YAMLAKTGVH HYSGNNIELG TACGKYYRVC TLAIIDPGD SDIIRSMP

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Macromolecule #28: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 12.506581 KDa
SequenceString:
NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLV TYVPVTTFKN LQTVNVD

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Macromolecule #29: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 15.179215 KDa
SequenceString:
AALRPLVKPK IVKKRTKKFI RHQSDRYVKI KRNWRKPRGI DNRVRRRFKG QILMPNIGYG SNKKTKHMLP SGFRKFLVHN VKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARLR

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Macromolecule #30: 60S ribosomal protein L35a

MacromoleculeName: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 12.449612 KDa
SequenceString:
SGRLWCKAIF AGYKRGLRNQ REHTALLKIE GVYARDETEF YLGKRCAYVY KAKNNTVTPG GKPNKTRVIW GKVTRAHGNS GMVRAKFRS NLPAKAIGHR IRVMLYPSRI

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Macromolecule #31: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 12.993569 KDa
SequenceString:
VQRLTYRRRL SYNTASNKTR LSRTPGNRIV YLYTKKVGKA PKSACGVCPG RLRGVRAVRP KVLMRLSKTQ KHVSRAYGGS MCAKCVRDR IKRAFLIEEQ KIVVKVLKAQ AQSQK

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Macromolecule #32: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 14.464507 KDa
SequenceString:
AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLTKHEE KLKTKKQQRK ERLYPLRKYA VKA

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Macromolecule #33: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 11.489695 KDa
SequenceString:
RYPMAVGLNK GHKVTKNVSK PRHSRRRSRL TNHTKFVRDM IREVCGFAPY ERRAMELLKV SKSKRALKFI KKRVGTHIRA KRKREELSN VLAAMEEAA

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Macromolecule #34: 60S ribosomal protein L37

MacromoleculeName: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 9.864596 KDa
SequenceString:
TKGTSSFGKR RNKTHTLCRR CGSKAYHLQK STCGKCGYPA KRKRKYNWSA KAKRRNTTGT GRMRHLKIVY RRFRHGFREG TTPK

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Macromolecule #35: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 8.093726 KDa
SequenceString:
PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKDLK

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Macromolecule #36: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 6.295562 KDa
SequenceString:
SSHKTFRIKR FLAKKQKQNR PIPQWIRMKT GNKIRYNSKR RHWRRTKLGL

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Macromolecule #37: 60S ribosomal protein L40

MacromoleculeName: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 5.957237 KDa
SequenceString:
IEPSLRQLAQ KYNCDKMICR KCYARLHPRA VNCRKKKCGH TNNLRPKKKV

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Macromolecule #38: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

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Macromolecule #39: Ribosomal protein L36A

MacromoleculeName: Ribosomal protein L36A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 12.345776 KDa
SequenceString:
VNVPKTRRTF CKKCGKHQPH KVTQYKKGKD SLYAQGKRRY DRKQSGYGGQ TKPIFRKKAK TTKKIVLRLE CVEPNCRSKR MLAIKRCKH FELGGDKKRK GQVIQF

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Macromolecule #40: Ribosomal protein L37a

MacromoleculeName: Ribosomal protein L37a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 10.168153 KDa
SequenceString:
AKRTKKVGIV GKYGTRYGAS LRKMVKKIEI SQHAKYTCSF CGKTKMKRRA VGIWHCGSCM KTVAGGAWTY NTTSAVTVKS AIRRLKELK DQ

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Macromolecule #41: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 22.097578 KDa
SequenceString:
SMLRLQKRLA SSVLRCGKKK VWLDPNETNE IANANSRQQI RKLIKDGLII RKPVTVHSRA RCRKNTLARR KGRHMGIGKR KGTANARMP EKVTWMRRMR ILRRLLRRYR ESKKIDRHMY HSLYLKVKGN VFKNKRILME HIHKLKADKA RKKLLADQAE A RRSKTKEA RKRREERLQA KKEEII

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Macromolecule #42: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 14.009404 KDa
SequenceString:
SAHLQWMVVR NCSSFLIKRN KQTYSTEPNN LKARNSFRYN GLIHRKTVGV EPAADGKGVV VVMKRRSGQR KPATSYVRTT INKNARATL SSIRHMIRKN KYRPDLRMAA IRRASAILRS QKP

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Macromolecule #46: Proliferation-associated protein 2G4

MacromoleculeName: Proliferation-associated protein 2G4 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 39.388098 KDa
SequenceString: QEQTIAEDLV VTKYKMGGDI ANRVLRSLVE ASSSGVSVLS LCEKGDAMIM EETGKIFKKE KEMKKGIAFP TSISVNNCVC HFSPLKSDQ DYILKEGDLV KIDLGVHVDG FIANVAHTFV IGVAQGTQVT GRKADVIKAA HLCAEAALRL VKPGNQNTQV T EAWNKVAH ...String:
QEQTIAEDLV VTKYKMGGDI ANRVLRSLVE ASSSGVSVLS LCEKGDAMIM EETGKIFKKE KEMKKGIAFP TSISVNNCVC HFSPLKSDQ DYILKEGDLV KIDLGVHVDG FIANVAHTFV IGVAQGTQVT GRKADVIKAA HLCAEAALRL VKPGNQNTQV T EAWNKVAH SFNCTPIEGM LSHQLKQHVI DGEKTIIQNP TDQQKKDHEK AEFEVHEVYA VDVLVSSGEG KAKDAGQRTT IY KRDPSKQ YGLKMKTSRA FFSEVERRFD AMPFTLRAFE DEKKARMGVV ECAKHELLQP FNVLYEKEGE FVAQFKFTVL LMP NGPMRI TSGPFEPDLY KSEMEVQDAE LKALLQS

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Macromolecule #43: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 43
Details: We used the Oryctolagus cuniculus sequence to model the Mus musculus 28S ribosomal RNA
Number of copies: 1
Source (natural)Organism: Mouse (mice)
Molecular weightTheoretical: 1.170200875 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC GCGCGGGGCG UGGGAAAUGU GGCGUACGGA A GACCCACU ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC GCGCGGGGCG UGGGAAAUGU GGCGUACGGA A GACCCACU CCCCGGCGCC GCUCGUGGGG GGCCCAAGUC CUUCUGAUCG AGGCCCAGCC CGUGGACGGU GUGAGGCCGG UA GCGGCCC CGGCGCCGGG CUCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG GGUCC GGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCC CCCGGGGUCG GCGGGGGACC GUCCCCCGGA CCGGCGACCG GCCGCC GCC GGGCGCAUUU CCAGGCGGUG CGCCGCGACC GGCUCCGGGA CGGCUGGGAA GGCCCGGCGG GGAAGGUGGC UCGGGGC CC CCGAGUGUUA CAGCCCCCCC GGCAGCAGCA CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUCGCC GCGCUCUC C CCCCUGGGGG GGCCGGGCCA CCCCUCCCAC GGCGCGACCG CUCUCCCAGG GGGCGGGGCG GACUGUCCCC AGUGCGCCC CGGGCGGGUC GCGCCGUCGG GCCCGGGGGG CCACGCGCGC GUCCCGGGAC GGCGGAGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUA CCCACCCGAC CCGUCUUGAA ACACGGACCA AGGAGUCUAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA G CCGCCGUG GCGCAAUGAA GGUGAAGGCC GGCGCGCUCG CCGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GG GGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACCCG AAAGAUGGUG AAC UAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAAA UCGGUCGUCC GACC UGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGGC GCUCU CGCC CACGCAGUUU UAUCCGGUAA AGCGAAUGAU UAGAGGUCUU GGGGCCGAAA CGAUCUCAAC CUAUUCUCAA ACUUUA AAU GGGUAAGAAG CCCGGCUCGC UGGCGUGGAG CCGGGGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAA CU GGCGCUGCGG GAUGAACCGA ACGCCGGGUU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUU G AUAUAGACAG CAGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCGUCGG GCCCAUACCC GGCCGUCGCC GGCCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAG CCUUGAAGCC UAGGGCGCGG GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU U CAAACGAG AACUUUGAAG GCCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AG AUGGGCG AGCGCCGUUC CGAAGGGACG GGCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCC CCGAAU CCGGAGUGGC GGAGAUGGGC GCCGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAG CCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUU GGAA AGCGUCGCAC CUUUGCUGGG GUCCGGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGC GCC GGGCCGUACC CAUAUCCGCA GCAGGUCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGU CG GCAAGCCGGA UCCGUAACUU CGGGAUAAGG AUUGGCUCUA AGGGCUGGCA GCCGACUUAG AACUGGUGCG GACCAGGG G AAUCCGACUG UUUAAUUAAA ACAAAGCAUC GCGAAGGCCC GCGGCGGGUG UUGACGCGAU GUGAUUUCUG CCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAAUGAA GCGCGGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUGAAC GAGAUUCCCA CUGUCCCUAC CUACUAUCCA GCGAAACCAC A GCCAAGGG AACGGGCUUG GCGGAAUCAG CGGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUCUGGC ACGGUGAAGA GA CAUGAGA GGUGUAGAAU AAGUGGGAGG CCCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGU GAGGCG GGGGGGCGAG CCCGAGGGGC UCUCGCUUCU GGCGCCAAGC GCCCGCCCGG CCGGGCGCGA CCCGCUCCGG GGAC AGUGC CAGGUGGGGA GUUUGACUGG GGCGGUACAC CUGUCAAACG GUAACGCAGG UGUCCUAAGG CGAGCUCAGG GAGGA CAGA AACCUCCCGU GGAGCAGAAG GGCAAAAGCU CGCUUGAUCU UGAUUUUCAG UACGAAUACA GACCGUGAAA GCGGGG CCU CACGAUCCUU CUGACCUUUU GGGUUUUAAG CAGGAGGUGU CAGAAAAGUU ACCACAGGGA UAACUGGCUU GUGGCGG CC AAGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUUGUGA AGCAGAAUUC GCCAAGCG U UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGA UGUGUUGUUG CCAUGGUAAU CCUGCUCAGU ACGAGAGGAA CCGCAGGUUC AGACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGG GCGAAGCUAC CAUCUGUGGG AUUAUGACUG AACGCCUCUA AGUCAGAAUC CCGCCCAGGC GGAACGAUAC G GCAGCGCC GCGGAGCCUC GGUUGGCCUC GGAUAGCCGG UCCGGGGUCC GGUGCGGAGU GCCCUUCGUC CUGGGAAACG GG GCGCGGC CGGAAAGGCG GCCGCCCCCU CGCCCGUCAC GCACCGCACG UUCGUGGGGA ACCUGGCGCU AAACCAUUCG UAG ACGACC UGCUUCUGGG UCGGGGUUUC GUACGUAGCA GAGCAGCUCC CUCGCUGCGA UCUAUUGAAA GUCAGCCCUC GACA CAAGG GUUUGUC

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Macromolecule #44: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 44
Details: We used the Oryctolagus cuniculus sequence to model the Mus musculus 5.8S ribosomal RNA
Number of copies: 1
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 50.449812 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

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Macromolecule #45: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 45
Details: We used the Oryctolagus cuniculus sequence to model the Mus musculus 5S ribosomal RNA
Number of copies: 1
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 38.38575 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU

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Macromolecule #47: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 47 / Number of copies: 247 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #48: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 48 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
Component:
ConcentrationNameFormula
20.0 mMHEPES
100.0 mMpotassium chlorideKCl
10.0 mMmagnesium chlorideMgCl2
20.0 mMDithiothreitol
0.04 mMSpermine
0.5 mMSpermidine
0.1 mg/mlcycloheximide
GridModel: Quantifoil R3/3 / Material: COPPER / Mesh: 100 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2.0 nm / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 31000
Specialist opticsEnergy filter - Name: GIF Quantum LS
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 5379 / Average exposure time: 20.0 sec. / Average electron dose: 31.78 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 208206
CTF correctionSoftware - Name: CTFFIND
Initial angle assignmentType: COMMON LINE / Software - Name: SPHIRE
Final 3D classificationSoftware - Name: SPARX
Final angle assignmentType: COMMON LINE / Software - Name: SPARX
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: SPARX / Number images used: 208206

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