National Natural Science Foundation of China (NSFC)
31930064
中国
National Natural Science Foundation of China (NSFC)
31970264
中国
National Natural Science Foundation of China (NSFC)
31770778
中国
引用
ジャーナル: Mol Plant / 年: 2022 タイトル: Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana. 著者: Xiaodong Su / Duanfang Cao / Xiaowei Pan / Lifang Shi / Zhenfeng Liu / Luca Dall'Osto / Roberto Bassi / Xinzheng Zhang / Mei Li / 要旨: Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane- ...Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.9 Å resolution structure of the Arabidopsis thaliana NDH-PSI (AtNDH-PSI) supercomplex. We constructed structural models for 26 AtNDH subunits, among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex. In the supercomplex, one NDH can bind up to two PSI-light-harvesting complex I (PSI-LHCI) complexes at both sides of its membrane arm. Two minor LHCIs, Lhca5 and Lhca6, each present in one PSI-LHCI, interact with NDH and contribute to supercomplex formation and stabilization. Collectively, our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (8)
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集合体
登録構造単位
AA: Photosystem I P700 chlorophyll a apoprotein A1 AB: Photosystem I P700 chlorophyll a apoprotein A2 AC: Photosystem I iron-sulfur center AD: Photosystem I reaction center subunit II-2, chloroplastic AE: Photosystem I reaction center subunit IV A, chloroplastic AF: Photosystem I reaction center subunit III, chloroplastic AG: Photosystem I reaction center subunit V, chloroplastic AH: Photosystem I reaction center subunit VI-2, chloroplastic AI: Photosystem I reaction center subunit VIII AJ: Photosystem I reaction center subunit IX AK: Photosystem I reaction center subunit psaK, chloroplastic AL: Photosystem I reaction center subunit XI, chloroplastic A1: Chlorophyll a-b binding protein 6, chloroplastic A3: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic A4: Chlorophyll a-b binding protein 4, chloroplastic A6: Photosystem I chlorophyll a/b-binding protein 6, chloroplastic BA: Photosystem I P700 chlorophyll a apoprotein A1 BB: Photosystem I P700 chlorophyll a apoprotein A2 BC: Photosystem I iron-sulfur center BD: Photosystem I reaction center subunit II-2, chloroplastic BE: Photosystem I reaction center subunit IV A, chloroplastic BF: Photosystem I reaction center subunit III, chloroplastic BG: Photosystem I reaction center subunit V, chloroplastic BH: Photosystem I reaction center subunit VI-2, chloroplastic BI: Photosystem I reaction center subunit VIII BJ: Photosystem I reaction center subunit IX BK: Photosystem I reaction center subunit psaK, chloroplastic BL: Photosystem I reaction center subunit XI, chloroplastic B1: Chlorophyll a-b binding protein 6, chloroplastic B2: Photosystem I chlorophyll a/b-binding protein 2, chloroplastic B3: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic B5: Photosystem I chlorophyll a/b-binding protein 5, chloroplastic A: NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic B: NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic C: NAD(P)H-quinone oxidoreductase subunit 3, chloroplastic D: NAD(P)H-quinone oxidoreductase chain 4, chloroplastic E: NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic F: NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic G: NAD(P)H-quinone oxidoreductase subunit 6, chloroplastic a: Photosynthetic NDH subunit of subcomplex B 1, chloroplastic b: Photosynthetic NDH subunit of subcomplex B 2, chloroplastic c: Photosynthetic NDH subunit of subcomplex B 3, chloroplastic d: NDH dependent flow 6 e: Photosynthetic NDH subunit of subcomplex B 5, chloroplastic f: Photosynthetic NDH subunit of lumenal location 1, chloroplastic g: Photosynthetic NDH subunit of lumenal location 2, chloroplastic h: Photosynthetic NDH subunit of lumenal location 3, chloroplastic i: Photosynthetic NDH subunit of lumenal location 4, chloroplastic j: Isoform 2 of Photosynthetic NDH subunit of lumenal location 5, chloroplastic H: NAD(P)H-quinone oxidoreductase subunit H, chloroplastic I: NAD(P)H-quinone oxidoreductase subunit I, chloroplastic J: NAD(P)H-quinone oxidoreductase subunit J, chloroplastic K: NAD(P)H-quinone oxidoreductase subunit K, chloroplastic L: NAD(P)H-quinone oxidoreductase subunit L, chloroplastic M: NAD(P)H-quinone oxidoreductase subunit M, chloroplastic N: NAD(P)H-quinone oxidoreductase subunit N, chloroplastic O: NdhO T: NdhT ヘテロ分子
分子量: 10400.812 Da / 分子数: 1 / 由来タイプ: 天然 詳細: All Residues in NdhT were modeled as UNK. The real sequence of the entity is: ...詳細: All Residues in NdhT were modeled as UNK. The real sequence of the entity is: MAYATSTYARTSCIILPKIQNGAHFTDNTKAFRRITARRVTRISSQGPTKPPKPSPGVDTRIHWESPDEGWIGGRSDPAKSVDEDKTNLLSDEKFAELIKDSFDSHYQFLGVSTDAHLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYNVLSDEETRRFYDWTLAQEVASRQAEKMRMKLEDPKEQDFRGYESIPDMVDRLGGRNMELSDQAMTALTFDILIVLFAVCCIVFVIVFKDPSY 由来: (天然) Arabidopsis thaliana (シロイヌナズナ)
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Photosystem I reaction center subunit ... , 9種, 18分子 ADBDAEBEAFBFAGBGAHBHAIBIAJBJAKBKALBL