+Open data
-Basic information
Entry | Database: PDB / ID: 7ej2 | ||||||
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Title | human voltage-gated potassium channel KV1.3 H451N mutant | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / potassium channel / complex / C-type inactivation | ||||||
Function / homology | Function and homology information pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / delayed rectifier potassium channel activity / Voltage gated Potassium channels / outward rectifier potassium channel activity ...pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / delayed rectifier potassium channel activity / Voltage gated Potassium channels / outward rectifier potassium channel activity / juxtaparanode region of axon / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / regulation of potassium ion transmembrane transport / optic nerve development / action potential / tertiary granule membrane / calyx of Held / voltage-gated potassium channel activity / potassium channel regulator activity / specific granule membrane / voltage-gated potassium channel complex / potassium ion transmembrane transport / potassium ion transport / protein homooligomerization / cytoplasmic side of plasma membrane / presynaptic membrane / postsynaptic membrane / transmembrane transporter binding / cytoskeleton / membrane raft / axon / glutamatergic synapse / Neutrophil degranulation / synapse / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.3 Å | ||||||
Authors | Liu, S. / Zhao, Y. / Tian, C. | ||||||
Citation | Journal: Cell Discov / Year: 2021 Title: Structures of wild-type and H451N mutant human lymphocyte potassium channel K1.3. Authors: Sanling Liu / Yue Zhao / Hao Dong / Liang Xiao / Yong Zhang / Yuqin Yang / Seow Theng Ong / K George Chandy / Longhua Zhang / Changlin Tian / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7ej2.cif.gz | 489.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7ej2.ent.gz | 383.3 KB | Display | PDB format |
PDBx/mmJSON format | 7ej2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7ej2_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 7ej2_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 7ej2_validation.xml.gz | 78.2 KB | Display | |
Data in CIF | 7ej2_validation.cif.gz | 118.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ej/7ej2 ftp://data.pdbj.org/pub/pdb/validation_reports/ej/7ej2 | HTTPS FTP |
-Related structure data
Related structure data | 31149MC 7ej1C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 41049.152 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCNAB2, KCNA2B, KCNK2 / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: Q13303, Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor #2: Protein | Mass: 63882.434 Da / Num. of mol.: 4 / Mutation: H451N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCNA3, HGK5 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P22001 #3: Chemical | ChemComp-NAP / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Human KV1.3 H451N-KVb2.1 / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 62 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.3 Å / Num. of particles: 79338 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 101.5 Å2 | ||||||||||||||||||||||||
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