Mass: 369.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C17H20ClNO4S / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
2
isotropic
1
2D 1H-13C HSQC
1
3
2
isotropic
1
3D 1H-13C NOESY
-
Sample preparation
Details
Type
Solution-ID
Contents
Label
Solvent system
solution
1
0.5 mM [U-15N] cChimera_protein, 100 mM potassium chloride, 10 mM imidazole, 10 mM calcium chloride, 0.25 mM [U-99% 2H] DSS, 1.5 mM A7, 1 % [U-99% 2H] DMSO, 95% H2O/5% D2O
15N_chimera
95% H2O/5% D2O
solution
2
0.5 mM [U-15N] cChimera_protein, 100 mM potassium chloride, 10 mM imidazole, 10 mM calcium chloride, 0.25 mM [U-99% 2H] DSS, 0.7 mM A7, 1 mM [U-99% 2H] DMSO, 100% D2O
13C_15N_chimera
100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.5mM
cChimera_protein
[U-15N]
1
100mM
potassiumchloride
naturalabundance
1
10mM
imidazole
naturalabundance
1
10mM
calciumchloride
naturalabundance
1
0.25mM
DSS
[U-99% 2H]
1
1.5mM
A7
naturalabundance
1
1 %
DMSO
[U-99% 2H]
1
0.5mM
cChimera_protein
[U-15N]
2
100mM
potassiumchloride
naturalabundance
2
10mM
imidazole
naturalabundance
2
10mM
calciumchloride
naturalabundance
2
0.25mM
DSS
[U-99% 2H]
2
0.7mM
A7
naturalabundance
2
1mM
DMSO
[U-99% 2H]
2
Sample conditions
Ionic strength: NA Not defined / Label: NMR_sample / pH: 6.7 / PH err: 0.1 / Pressure: ambient / Temperature: 303 K
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