+Open data
-Basic information
Entry | Database: PDB / ID: 6fit | ||||||
---|---|---|---|---|---|---|---|
Title | FHIT-TRANSITION STATE ANALOG | ||||||
Components | FRAGILE HISTIDINE TRIAD PROTEIN | ||||||
Keywords | HYDROLASE / FRAGILE HISTIDINE TRIAD PROTEIN / FHIT / PUTATIVE TUMOR SUPPRESSOR / HIT PROTEIN FAMILY / HISTIDINE TRIAD PROTEIN FAMILY / NUCLEOTIDYL HYDROLASE / NUCLEOTIDYL TRANSFERASE | ||||||
Function / homology | Function and homology information adenylylsulfate-ammonia adenylyltransferase / adenylylsulfatase / diadenosine triphosphate catabolic process / adenylylsulfate-ammonia adenylyltransferase activity / adenylylsulfatase activity / bis(5'-adenosyl)-triphosphatase / bis(5'-adenosyl)-triphosphatase activity / purine nucleotide metabolic process / Hydrolases; Acting on phosphorus-nitrogen bonds / adenosine 5'-monophosphoramidase activity ...adenylylsulfate-ammonia adenylyltransferase / adenylylsulfatase / diadenosine triphosphate catabolic process / adenylylsulfate-ammonia adenylyltransferase activity / adenylylsulfatase activity / bis(5'-adenosyl)-triphosphatase / bis(5'-adenosyl)-triphosphatase activity / purine nucleotide metabolic process / Hydrolases; Acting on phosphorus-nitrogen bonds / adenosine 5'-monophosphoramidase activity / intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / fibrillar center / nucleotide binding / ubiquitin protein ligase binding / mitochondrion / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6 Å | ||||||
Authors | Lima, C.D. / Klein, M.G. / Hendrickson, W.A. | ||||||
Citation | Journal: Science / Year: 1997 Title: Structure-based analysis of catalysis and substrate definition in the HIT protein family. Authors: Lima, C.D. / Klein, M.G. / Hendrickson, W.A. #1: Journal: Structure / Year: 1997 Title: MAD Analysis of Fhit, a Putative Human Tumor Suppressor from the Hit Protein Family Authors: Lima, C.D. / D'Amico, K.L. / Naday, I. / Rosenbaum, G. / Westbrook, E.M. / Hendrickson, W.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6fit.cif.gz | 39.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6fit.ent.gz | 27.2 KB | Display | PDB format |
PDBx/mmJSON format | 6fit.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6fit_validation.pdf.gz | 437.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6fit_full_validation.pdf.gz | 441.1 KB | Display | |
Data in XML | 6fit_validation.xml.gz | 5 KB | Display | |
Data in CIF | 6fit_validation.cif.gz | 6.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fi/6fit ftp://data.pdbj.org/pub/pdb/validation_reports/fi/6fit | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 16886.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: EXPRESSED AS FUSION PROTEIN WITH GLUTATHIONE-S-TRANSFERASE IN ESCHERICHIA COLI Gene: FHIT / Plasmid: PGEX-2T / Gene (production host): FHIT / Production host: Escherichia coli (E. coli) / Strain (production host): DH5-ALPHA References: UniProt: P49789, bis(5'-adenosyl)-triphosphatase |
---|---|
#2: Chemical | ChemComp-AMW / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.04 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 6.5 / Details: GROWN FROM AMMONIUM SULFATE, PH 6.5 | ||||||||||||||||||||
Crystal grow | *PLUS Method: unknown / Details: Lima, C.D., (1997) Structure (London), 5, 763. | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 110 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 1.074 |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Nov 15, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.074 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 17675 / % possible obs: 79.6 % / Observed criterion σ(I): 2 / Redundancy: 8 % / Rmerge(I) obs: 0.064 |
Reflection | *PLUS Num. measured all: 167167 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.6→10 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / σ(F): 2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|