登録情報 データベース : EMDB / ID : EMD-6987 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of a P-type ATPase マップデータ 詳細 試料複合体 : complex of one PMCA1 molecular with one NPTN molecularタンパク質・ペプチド : Plasma membrane calcium-transporting ATPase 1タンパク質・ペプチド : Neuroplastinリガンド : N-ACETYL-D-GLUCOSAMINE 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration / regulation of receptor localization to synapse / calcium ion export across plasma membrane / calcium ion transmembrane transporter activity / : / trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission / regulation of vascular associated smooth muscle contraction / type 1 fibroblast growth factor receptor binding / : / excitatory synapse assembly ... P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration / regulation of receptor localization to synapse / calcium ion export across plasma membrane / calcium ion transmembrane transporter activity / : / trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission / regulation of vascular associated smooth muscle contraction / type 1 fibroblast growth factor receptor binding / : / excitatory synapse assembly / cellular response to corticosterone stimulus / : / dendrite self-avoidance / positive regulation of fibroblast growth factor receptor signaling pathway / inhibitory synapse / positive regulation of long-term neuronal synaptic plasticity / GABA receptor activation / cell-cell adhesion mediator activity / P-type Ca2+ transporter / neural retina development / P-type calcium transporter activity / cellular response to vitamin D / ATPase-coupled monoatomic cation transmembrane transporter activity / positive regulation of calcium ion transport / dendritic spine membrane / Reduction of cytosolic Ca++ levels / negative regulation of cytokine production / positive regulation of protein localization / neuronal cell body membrane / negative regulation of cytosolic calcium ion concentration / plasma membrane => GO:0005886 / homophilic cell adhesion via plasma membrane adhesion molecules / Ion transport by P-type ATPases / immunological synapse / regulation of cardiac conduction / GABA-ergic synapse / positive regulation of bone mineralization / regulation of cellular response to insulin stimulus / Ion homeostasis / monoatomic ion transmembrane transport / cell adhesion molecule binding / regulation of cytosolic calcium ion concentration / response to cold / positive regulation of long-term synaptic potentiation / PDZ domain binding / long-term synaptic potentiation / axon guidance / Schaffer collateral - CA1 synapse / visual learning / brain development / cytoplasmic side of plasma membrane / regulation of blood pressure / positive regulation of neuron projection development / intracellular calcium ion homeostasis / presynaptic membrane / positive regulation of cytosolic calcium ion concentration / basolateral plasma membrane / transmembrane transporter binding / positive regulation of ERK1 and ERK2 cascade / calmodulin binding / positive regulation of protein phosphorylation / membrane raft / apical plasma membrane / axon / intracellular membrane-bounded organelle / glutamatergic synapse / dendrite / cell surface / ATP hydrolysis activity / extracellular exosome / nucleoplasm / ATP binding / membrane / metal ion binding / plasma membrane 類似検索 - 分子機能 Plasma membrane calcium-transporting ATPase 1 / Neuroplastin / Plasma membrane calcium transporting P-type ATPase, C-terminal / Plasma membrane calcium transporter ATPase C terminal / P-type ATPase, subfamily IIB / Basigin-like / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal ... Plasma membrane calcium-transporting ATPase 1 / Neuroplastin / Plasma membrane calcium transporting P-type ATPase, C-terminal / Plasma membrane calcium transporter ATPase C terminal / P-type ATPase, subfamily IIB / Basigin-like / Cation-transporting P-type ATPase, C-terminal / Cation transporting ATPase, C-terminus / Cation transporter/ATPase, N-terminus / Cation-transporting P-type ATPase, N-terminal / Cation transporter/ATPase, N-terminus / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold 類似検索 - ドメイン・相同性 Plasma membrane calcium-transporting ATPase 1 / Neuroplastin 類似検索 - 構成要素生物種 Homo sapiens (ヒト) / Human (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4.11 Å 詳細 データ登録者Gong DS / Chi XM / Ren K / Huang GXY / Zhou GW / Yan N / Lei JL / Zhou Q 引用ジャーナル : Nat Commun / 年 : 2018タイトル : Structure of the human plasma membrane Ca-ATPase 1 in complex with its obligatory subunit neuroplastin.著者 : Deshun Gong / Ximin Chi / Kang Ren / Gaoxingyu Huang / Gewei Zhou / Nieng Yan / Jianlin Lei / Qiang Zhou / 要旨 : Plasma membrane Ca-ATPases (PMCAs) are key regulators of global Ca homeostasis and local intracellular Ca dynamics. Recently, Neuroplastin (NPTN) and basigin were identified as previously ... Plasma membrane Ca-ATPases (PMCAs) are key regulators of global Ca homeostasis and local intracellular Ca dynamics. Recently, Neuroplastin (NPTN) and basigin were identified as previously unrecognized obligatory subunits of PMCAs that dramatically increase the efficiency of PMCA-mediated Ca clearance. Here, we report the cryo-EM structure of human PMCA1 (hPMCA1) in complex with NPTN at a resolution of 4.1 Å for the overall structure and 3.9 Å for the transmembrane domain. The single transmembrane helix of NPTN interacts with the TM-linker and TM10 of hPMCA1. The subunits are required for the hPMCA1 functional activity. The NPTN-bound hPMCA1 closely resembles the E1-Mg structure of endo(sarco)plasmic reticulum Ca ATPase and the Ca site is exposed through a large open cytoplasmic pathway. This structure provides insight into how the subunits bind to the PMCAs and serves as an important basis for understanding the functional mechanisms of this essential calcium pump family. 履歴 登録 2018年6月27日 - ヘッダ(付随情報) 公開 2018年9月19日 - マップ公開 2018年9月19日 - 更新 2018年9月19日 - 現状 2018年9月19日 処理サイト : PDBj / 状態 : 公開
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