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基本情報
登録情報 | データベース: EMDB / ID: EMD-6839 | ||||||||||||
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タイトル | Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom | ||||||||||||
![]() | Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom | ||||||||||||
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![]() | Post-catalytic Spliceosome (P complex) / RNA splicing / exon ligation / spliceosome / SPLICING | ||||||||||||
機能・相同性 | ![]() U2-type post-spliceosomal complex / mRNA branch site recognition / spliceosomal complex disassembly / U2-type post-mRNA release spliceosomal complex / cellular bud site selection / pre-mRNA 3'-splice site binding / post-mRNA release spliceosomal complex / nuclear mRNA surveillance / generation of catalytic spliceosome for first transesterification step / cis assembly of pre-catalytic spliceosome ...U2-type post-spliceosomal complex / mRNA branch site recognition / spliceosomal complex disassembly / U2-type post-mRNA release spliceosomal complex / cellular bud site selection / pre-mRNA 3'-splice site binding / post-mRNA release spliceosomal complex / nuclear mRNA surveillance / generation of catalytic spliceosome for first transesterification step / cis assembly of pre-catalytic spliceosome / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / pre-mRNA binding / snRNP binding / small nuclear ribonucleoprotein complex / splicing factor binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / commitment complex / mRNA cis splicing, via spliceosome / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / poly(U) RNA binding / U2-type prespliceosome / precatalytic spliceosome / generation of catalytic spliceosome for second transesterification step / Formation of TC-NER Pre-Incision Complex / spliceosomal complex assembly / mRNA 5'-splice site recognition / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA 3'-splice site recognition / DNA replication origin binding / spliceosomal tri-snRNP complex assembly / Dual incision in TC-NER / Prp19 complex / protein K63-linked ubiquitination / U5 snRNA binding / U5 snRNP / DNA replication initiation / U2 snRNA binding / U6 snRNA binding / pre-mRNA intronic binding / spliceosomal snRNP assembly / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / positive regulation of cell cycle / catalytic step 2 spliceosome / nuclear periphery / positive regulation of RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / RING-type E3 ubiquitin transferase / metallopeptidase activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / RNA helicase activity / RNA helicase / DNA repair / mRNA binding / GTPase activity / chromatin binding / chromatin / GTP binding / ATP hydrolysis activity / mitochondrion / DNA binding / RNA binding / zinc ion binding / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | ||||||||||||
![]() | Wan R / Yan C | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae. 著者: Rui Bai / Chuangye Yan / Ruixue Wan / Jianlin Lei / Yigong Shi / ![]() 要旨: Removal of an intron from a pre-mRNA by the spliceosome results in the ligation of two exons in the post-catalytic spliceosome (known as the P complex). Here, we present a cryo-EM structure of the ...Removal of an intron from a pre-mRNA by the spliceosome results in the ligation of two exons in the post-catalytic spliceosome (known as the P complex). Here, we present a cryo-EM structure of the P complex from Saccharomyces cerevisiae at an average resolution of 3.6 Å. The ligated exon is held in the active site through RNA-RNA contacts. Three bases at the 3' end of the 5' exon remain anchored to loop I of U5 small nuclear RNA, and the conserved AG nucleotides of the 3'-splice site (3'SS) are specifically recognized by the invariant adenine of the branch point sequence, the guanine base at the 5' end of the 5'SS, and an adenine base of U6 snRNA. The 3'SS is stabilized through an interaction with the 1585-loop of Prp8. The P complex structure provides a view on splice junction formation critical for understanding the complete splicing cycle. | ||||||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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画像 | ![]() | 153.3 KB | ||
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その他 | ![]() | 228.9 MB | ||
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文書・要旨 | ![]() | 603.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 603.1 KB | 表示 | |
XML形式データ | ![]() | 6.9 KB | 表示 | |
CIF形式データ | ![]() | 7.8 KB | 表示 | |
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-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
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注釈 | Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
+全体 : the Post-catalytic Spliceosome (P complex)
+超分子 #1: the Post-catalytic Spliceosome (P complex)
+分子 #1: Pre-mRNA-splicing factor 8
+分子 #3: Pre-mRNA-splicing factor SNU114
+分子 #7: Pre-mRNA-splicing factor SNT309
+分子 #8: Pre-mRNA-splicing factor SYF1
+分子 #9: Pre-mRNA-splicing factor CLF1
+分子 #10: Pre-mRNA-splicing factor CEF1
+分子 #11: Pre-mRNA-splicing factor SYF2
+分子 #12: Pre-mRNA-splicing factor BUD31
+分子 #13: Pre-mRNA-processing factor 17
+分子 #14: Pre-mRNA-splicing factor SLT11
+分子 #15: Pre-mRNA-splicing factor CWC2
+分子 #16: Pre-mRNA-splicing factor PRP46
+分子 #17: Pre-mRNA-splicing factor CWC15
+分子 #18: Pre-mRNA-processing protein 45
+分子 #19: Pre-mRNA-splicing factor CWC21
+分子 #20: Pre-mRNA-splicing factor CWC22
+分子 #21: Pre-mRNA-splicing factor 18
+分子 #22: Pre-mRNA-splicing factor SLU7
+分子 #23: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
+分子 #24: Small nuclear ribonucleoprotein-associated protein B
+分子 #25: Small nuclear ribonucleoprotein E
+分子 #26: Small nuclear ribonucleoprotein F
+分子 #27: Small nuclear ribonucleoprotein G
+分子 #28: Small nuclear ribonucleoprotein Sm D3
+分子 #29: Small nuclear ribonucleoprotein Sm D1
+分子 #30: Small nuclear ribonucleoprotein Sm D2
+分子 #31: U2 small nuclear ribonucleoprotein A'
+分子 #32: U2 small nuclear ribonucleoprotein B''
+分子 #33: Pre-mRNA-processing factor 19
+分子 #2: U5 snRNA
+分子 #4: U6 snRNA
+分子 #5: mRNA/intron lariat
+分子 #6: U2 snRNA
+分子 #34: INOSITOL HEXAKISPHOSPHATE
+分子 #35: GUANOSINE-5'-TRIPHOSPHATE
+分子 #36: MAGNESIUM ION
+分子 #37: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.9 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |