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- PDB-5ndz: Crystal structure of a thermostabilised human protease-activated ... -
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Basic information
Entry | Database: PDB / ID: 5ndz | ||||||
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Title | Crystal structure of a thermostabilised human protease-activated receptor-2 (PAR2) in complex with AZ3451 at 3.6 angstrom resolution | ||||||
![]() | Lysozyme,Proteinase-activated receptor 2,Soluble cytochrome b562,Proteinase-activated receptor 2 | ||||||
![]() | MEMBRANE PROTEIN / GPCR / 7TM | ||||||
Function / homology | ![]() positive regulation of neutrophil mediated killing of gram-negative bacterium / potassium channel activating, G protein-coupled receptor signaling pathway / positive regulation of renin secretion into blood stream / positive regulation of eosinophil degranulation / leukocyte proliferation / mature conventional dendritic cell differentiation / regulation of chemokine (C-X-C motif) ligand 2 production / positive regulation of glomerular filtration / negative regulation of toll-like receptor 3 signaling pathway / positive regulation of toll-like receptor 3 signaling pathway ...positive regulation of neutrophil mediated killing of gram-negative bacterium / potassium channel activating, G protein-coupled receptor signaling pathway / positive regulation of renin secretion into blood stream / positive regulation of eosinophil degranulation / leukocyte proliferation / mature conventional dendritic cell differentiation / regulation of chemokine (C-X-C motif) ligand 2 production / positive regulation of glomerular filtration / negative regulation of toll-like receptor 3 signaling pathway / positive regulation of toll-like receptor 3 signaling pathway / thrombin-activated receptor activity / positive regulation of toll-like receptor 2 signaling pathway / positive regulation of actin filament depolymerization / cell-cell junction maintenance / positive regulation of toll-like receptor 4 signaling pathway / positive regulation of cytokine production involved in immune response / positive regulation of pseudopodium assembly / T cell activation involved in immune response / neutrophil activation / negative regulation of chemokine production / positive regulation of phagocytosis, engulfment / positive regulation of leukocyte chemotaxis / positive regulation of chemotaxis / negative regulation of JNK cascade / establishment of endothelial barrier / regulation of JNK cascade / regulation of canonical NF-kappaB signal transduction / positive regulation of positive chemotaxis / positive regulation of Rho protein signal transduction / leukocyte migration / regulation of blood coagulation / pseudopodium / positive regulation of interleukin-10 production / negative regulation of tumor necrosis factor-mediated signaling pathway / G-protein alpha-subunit binding / negative regulation of insulin secretion / positive regulation of chemokine production / positive regulation of superoxide anion generation / viral release from host cell by cytolysis / peptidoglycan catabolic process / Peptide ligand-binding receptors / positive regulation of GTPase activity / positive regulation of interleukin-1 beta production / positive regulation of interleukin-8 production / G protein-coupled receptor activity / electron transport chain / positive regulation of JNK cascade / vasodilation / positive regulation of interleukin-6 production / positive regulation of type II interferon production / G-protein beta-subunit binding / blood coagulation / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / signaling receptor activity / phospholipase C-activating G protein-coupled receptor signaling pathway / positive regulation of canonical NF-kappaB signal transduction / positive regulation of cytosolic calcium ion concentration / G alpha (q) signalling events / protease binding / defense response to virus / host cell cytoplasm / positive regulation of ERK1 and ERK2 cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / periplasmic space / early endosome / electron transfer activity / positive regulation of cell migration / defense response to bacterium / inflammatory response / iron ion binding / G protein-coupled receptor signaling pathway / innate immune response / signaling receptor binding / heme binding / Golgi apparatus / positive regulation of transcription by RNA polymerase II / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cheng, R.K.Y. / Fiez-Vandal, C. / Schlenker, O. / Edman, K. / Aggeler, B. / Brown, D.G. / Brown, G. / Cooke, R.M. / Dumelin, C.E. / Dore, A.S. ...Cheng, R.K.Y. / Fiez-Vandal, C. / Schlenker, O. / Edman, K. / Aggeler, B. / Brown, D.G. / Brown, G. / Cooke, R.M. / Dumelin, C.E. / Dore, A.S. / Geschwindner, S. / Grebner, C. / Hermansson, N.-O. / Jazayeri, A. / Johansson, P. / Leong, L. / Prihandoko, R. / Rappas, M. / Soutter, H. / Snijder, A. / Sundstrom, L. / Tehan, B. / Thornton, P. / Troast, D. / Wiggin, G. / Zhukov, A. / Marshall, F.H. / Dekker, N. | ||||||
![]() | ![]() Title: Structural insight into allosteric modulation of protease-activated receptor 2. Authors: Cheng, R.K.Y. / Fiez-Vandal, C. / Schlenker, O. / Edman, K. / Aggeler, B. / Brown, D.G. / Brown, G.A. / Cooke, R.M. / Dumelin, C.E. / Dore, A.S. / Geschwindner, S. / Grebner, C. / ...Authors: Cheng, R.K.Y. / Fiez-Vandal, C. / Schlenker, O. / Edman, K. / Aggeler, B. / Brown, D.G. / Brown, G.A. / Cooke, R.M. / Dumelin, C.E. / Dore, A.S. / Geschwindner, S. / Grebner, C. / Hermansson, N.O. / Jazayeri, A. / Johansson, P. / Leong, L. / Prihandoko, R. / Rappas, M. / Soutter, H. / Snijder, A. / Sundstrom, L. / Tehan, B. / Thornton, P. / Troast, D. / Wiggin, G. / Zhukov, A. / Marshall, F.H. / Dekker, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 128.5 KB | Display | ![]() |
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PDB format | ![]() | 96.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5nddSC ![]() 5nj6C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 69558.898 Da / Num. of mol.: 1 Mutation: G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A,G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A,G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, ...Mutation: G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A,G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A,G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A,G89A, H108A, G157A, M166L, Y174A, V176E, N222Q, M268A, I289A, L293A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() Gene: e, T4Tp126, F2RL1, GPR11, PAR2, cybC / Plasmid: pFASTBAC / Production host: ![]() ![]() References: UniProt: D9IEF7, UniProt: P55085, UniProt: P0ABE7, UniProt: P00720*PLUS, lysozyme |
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#2: Chemical | ChemComp-8UN / |
#3: Chemical | ChemComp-NA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.08 Å3/Da / Density % sol: 60 % / Description: Needle-shaped crystals (on average 0.1mm long) |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase / pH: 5.8 Details: 0.1 M sodium citrate/citrate acid pH 5.5-6.2, 0.2 M ammonium phosphate dibasic, 38-43 % (w/v) PEG400 PH range: 5.5-6.2 / Temp details: CONSTANT |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96862 Å / Relative weight: 1 |
Reflection | Resolution: 3.59→34.441 Å / Num. obs: 8205 / % possible obs: 94.2 % / Redundancy: 3.5 % / Biso Wilson estimate: 24 Å2 / CC1/2: 0.888 / Rmerge(I) obs: 0.399 / Rpim(I) all: 0.233 / Net I/σ(I): 4.4 |
Reflection shell | Resolution: 3.59→3.94 Å / Redundancy: 3.5 % / Rmerge(I) obs: 1.186 / Mean I/σ(I) obs: 1.9 / Num. unique obs: 1993 / CC1/2: 0.457 / Rpim(I) all: 0.702 / % possible all: 95.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5NDD Resolution: 3.6→34.441 Å / SU ML: 0.64 / Cross valid method: FREE R-VALUE / σ(F): 1.92 / Phase error: 29.13
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.6→34.441 Å
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Refine LS restraints |
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LS refinement shell |
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