+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-5756 | |||||||||
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タイトル | 3D EM Reconstruction of the AKAP18-PKA Complex in a Linear Conformation | |||||||||
マップデータ | Reconstruction of AKAP18-PKA in a Linear Conformation | |||||||||
試料 |
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キーワード | A-Kinase Anchoring Protein / AKAP / AKAP18 / A-Kinase / PKA / cAMP-Dependent Kinase / Kinase / RII / PKA Regulatory Subunit II / Phosphorylation / Anchoring / Intrinsic Disorder | |||||||||
機能・相同性 | 機能・相同性情報 spontaneous exocytosis of neurotransmitter / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / negative regulation of meiotic cell cycle / HDL assembly / DARPP-32 events / Rap1 signalling / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / Regulation of insulin secretion ...spontaneous exocytosis of neurotransmitter / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / negative regulation of meiotic cell cycle / HDL assembly / DARPP-32 events / Rap1 signalling / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / Regulation of insulin secretion / GPER1 signaling / Hedgehog 'off' state / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / regulation of membrane repolarization / exocytic vesicle / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / MAPK6/MAPK4 signaling / GLI3 is processed to GLI3R by the proteasome / AURKA Activation by TPX2 / Factors involved in megakaryocyte development and platelet production / cAMP-dependent protein kinase regulator activity / Regulation of PLK1 Activity at G2/M Transition / Interleukin-3, Interleukin-5 and GM-CSF signaling / CD209 (DC-SIGN) signaling / Mitochondrial protein degradation / positive regulation of potassium ion transmembrane transport / RET signaling / nucleotide-activated protein kinase complex / regulation of protein kinase A signaling / Ion homeostasis / VEGFA-VEGFR2 Pathway / regulation of cellular respiration / regulation of protein processing / protein localization to lipid droplet / regulation of bicellular tight junction assembly / cellular response to parathyroid hormone stimulus / cAMP-dependent protein kinase inhibitor activity / cAMP-dependent protein kinase / cellular response to cold / protein kinase A binding / regulation of osteoblast differentiation / sperm capacitation / cAMP-dependent protein kinase activity / ciliary base / negative regulation of glycolytic process through fructose-6-phosphate / cAMP-dependent protein kinase complex / AMP-activated protein kinase activity / postsynaptic modulation of chemical synaptic transmission / cellular response to glucagon stimulus / AMP binding / protein kinase A regulatory subunit binding / plasma membrane raft / protein kinase A catalytic subunit binding / axoneme / mesoderm formation / lateral plasma membrane / small molecule binding / sperm flagellum / negative regulation of smoothened signaling pathway / regulation of proteasomal protein catabolic process / beta-2 adrenergic receptor binding / cAMP binding / cellular response to cAMP / regulation of synaptic transmission, glutamatergic / positive regulation of gluconeogenesis / sperm midpiece / negative regulation of TORC1 signaling / T-tubule / protein kinase A signaling / protein serine/threonine/tyrosine kinase activity / protein export from nucleus / hippocampal mossy fiber to CA3 synapse / positive regulation of protein export from nucleus / acrosomal vesicle / sarcoplasmic reticulum / neural tube closure / cellular response to glucose stimulus / regulation of protein phosphorylation / modulation of chemical synaptic transmission / neuromuscular junction / adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of insulin secretion / mRNA processing / small GTPase binding / presynapse / kinase activity / manganese ion binding / cellular response to heat / postsynapse / peptidyl-serine phosphorylation / dendritic spine / regulation of cell cycle / protein kinase activity / nuclear speck / phosphorylation / apical plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / Mus musculus (ハツカネズミ) | |||||||||
手法 | 単粒子再構成法 / ネガティブ染色法 / 解像度: 35.0 Å | |||||||||
データ登録者 | Smith FD / Reichow SL / Esseltine JL / Shi D / Langeberg LK / Scott JD / Gonen T | |||||||||
引用 | ジャーナル: Elife / 年: 2013 タイトル: Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation. 著者: F Donelson Smith / Steve L Reichow / Jessica L Esseltine / Dan Shi / Lorene K Langeberg / John D Scott / Tamir Gonen / 要旨: Anchoring proteins sequester kinases with their substrates to locally disseminate intracellular signals and avert indiscriminate transmission of these responses throughout the cell. Mechanistic ...Anchoring proteins sequester kinases with their substrates to locally disseminate intracellular signals and avert indiscriminate transmission of these responses throughout the cell. Mechanistic understanding of this process is hampered by limited structural information on these macromolecular complexes. A-kinase anchoring proteins (AKAPs) spatially constrain phosphorylation by cAMP-dependent protein kinases (PKA). Electron microscopy and three-dimensional reconstructions of type-II PKA-AKAP18γ complexes reveal hetero-pentameric assemblies that adopt a range of flexible tripartite configurations. Intrinsically disordered regions within each PKA regulatory subunit impart the molecular plasticity that affords an ∼16 nanometer radius of motion to the associated catalytic subunits. Manipulating flexibility within the PKA holoenzyme augmented basal and cAMP responsive phosphorylation of AKAP-associated substrates. Cell-based analyses suggest that the catalytic subunit remains within type-II PKA-AKAP18γ complexes upon cAMP elevation. We propose that the dynamic movement of kinase sub-structures, in concert with the static AKAP-regulatory subunit interface, generates a solid-state signaling microenvironment for substrate phosphorylation. DOI: http://dx.doi.org/10.7554/eLife.01319.001. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_5756.map.gz | 6.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-5756-v30.xml emd-5756.xml | 15.1 KB 15.1 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_5756.png | 61.5 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-5756 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-5756 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_5756_validation.pdf.gz | 297 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_5756_full_validation.pdf.gz | 296.6 KB | 表示 | |
XML形式データ | emd_5756_validation.xml.gz | 5.5 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-5756 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-5756 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_5756.map.gz / 形式: CCP4 / 大きさ: 6.4 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Reconstruction of AKAP18-PKA in a Linear Conformation | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 4.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : AKAP18-PKA Complex in Linear Conformation
全体 | 名称: AKAP18-PKA Complex in Linear Conformation |
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要素 |
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-超分子 #1000: AKAP18-PKA Complex in Linear Conformation
超分子 | 名称: AKAP18-PKA Complex in Linear Conformation / タイプ: sample / ID: 1000 集合状態: Hetero-pentamer composed of one AKAP18 bound to a dimer of the PKA Regulatory Subunit II and two copies of the PKA Catalytic Subunit Number unique components: 3 |
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分子量 | 実験値: 240 KDa / 理論値: 220 KDa / 手法: Native PAGE |
-分子 #1: A-Kinase Anchoring Protein 18
分子 | 名称: A-Kinase Anchoring Protein 18 / タイプ: protein_or_peptide / ID: 1 / Name.synonym: AKAP18 / コピー数: 1 / 集合状態: monomer / 組換発現: Yes |
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由来(天然) | 生物種: Homo sapiens (ヒト) / 別称: Human |
分子量 | 理論値: 40 KDa |
組換発現 | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) 組換株: Sf-9 / 組換プラスミド: Bacmid |
-分子 #2: A-Protein Kinase Catalytic Subunit
分子 | 名称: A-Protein Kinase Catalytic Subunit / タイプ: protein_or_peptide / ID: 2 / Name.synonym: PKA C subunit / コピー数: 2 / 集合状態: monomer / 組換発現: Yes |
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由来(天然) | 生物種: Mus musculus (ハツカネズミ) / 別称: Mouse |
分子量 | 理論値: 40 KDa |
組換発現 | 生物種: Escherichia coli (大腸菌) / 組換株: BL21(DE3)pLysS / 組換プラスミド: pET15a |
-分子 #3: A-Protein Kinase Regulatory Subunit II alpha
分子 | 名称: A-Protein Kinase Regulatory Subunit II alpha / タイプ: protein_or_peptide / ID: 3 / Name.synonym: PKA RIIalpha Subunit / コピー数: 2 / 集合状態: dimer / 組換発現: Yes |
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由来(天然) | 生物種: Mus musculus (ハツカネズミ) / 別称: Mouse |
分子量 | 理論値: 45 KDa |
組換発現 | 生物種: Escherichia coli (大腸菌) / 組換株: BL21(DE3)pLysS / 組換プラスミド: pET28b |
-実験情報
-構造解析
手法 | ネガティブ染色法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
濃度 | 0.01 mg/mL |
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緩衝液 | pH: 7.4 詳細: 25 mM HEPES, 200 mM NaCl, 0.5 mM EDTA, 1 mM dithiothreitol. |
染色 | タイプ: NEGATIVE / 詳細: 0.75% (w/v) uranyl formate |
グリッド | 詳細: 200 mesh copper grid with thin carbon support |
凍結 | 凍結剤: NONE / 装置: OTHER |
-電子顕微鏡法
顕微鏡 | FEI TECNAI 12 |
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温度 | 平均: 298 K |
アライメント法 | Legacy - 非点収差: Objective lens astigmatism was corrected at 100,000 times magnification |
日付 | 2012年5月1日 |
撮影 | カテゴリ: CCD / フィルム・検出器のモデル: GENERIC GATAN / 実像数: 1335 / 平均電子線量: 15 e/Å2 |
Tilt angle min | 0 |
電子線 | 加速電圧: 120 kV / 電子線源: LAB6 |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 6.3 mm / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.0 µm / 倍率(公称値): 52000 |
試料ステージ | 試料ホルダーモデル: OTHER / Tilt angle max: 50 |
-画像解析
詳細 | Images were processed in IMAGIC and ISAC. 3D reconstruction was done in IMAGIC and FREALIGN. |
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CTF補正 | 詳細: Each Micrograph |
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 35.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: IMAGIC, FREALIGN / 使用した粒子像数: 1000 |
-原子モデル構築 1
初期モデル | PDB ID: Chain - #0 - Chain ID: A / Chain - #1 - Chain ID: B |
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ソフトウェア | 名称: Chimera |
詳細 | This domain was linked to the AKAP18 central domain (PDB 2VFL) using COOT and they were fit together into the EM density. |
精密化 | 空間: REAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: cross-correlation |
得られたモデル | PDB-3j4r: |
-原子モデル構築 2
初期モデル | PDB ID: Chain - Chain ID: A |
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ソフトウェア | 名称: Chimera |
詳細 | This domain was linked to the AKAP RII Binding Domain (PDB 2IZX) using COOT and they were fit together into the EM density. |
精密化 | 空間: REAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: cross-correlation |
得られたモデル | PDB-3j4r: |
-原子モデル構築 3
初期モデル | PDB ID: Chain - #0 - Chain ID: A / Chain - #1 - Chain ID: B |
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ソフトウェア | 名称: Chimera |
詳細 | This domain was linked to the PKA RIIalpha D/D domain (PDB 2IZX) using COOT and they were fit together into the EM density. |
精密化 | 空間: REAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: cross-correlation |
得られたモデル | PDB-3j4r: |