登録情報 データベース : EMDB / ID : EMD-4396 構造の表示 ダウンロードとリンクタイトル Chromatin remodeller-nucleosome complex at 4.5 A resolution. マップデータChromatin remodeller-nucleosome complex at 4.5 A resolution. 詳細 試料複合体 : SWR1-nucleosome complex複合体 : Histones複合体 : DNA複合体 : remodellerリガンド : x 4種 詳細 キーワード Chromatin / Remodeller / ATPase / Histone / NUCLEAR PROTEIN機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
sexual sporulation resulting in formation of a cellular spore / HATs acetylate histones / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / R2TP complex / Assembly of the ORC complex at the origin of replication / Swr1 complex ... sexual sporulation resulting in formation of a cellular spore / HATs acetylate histones / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / R2TP complex / Assembly of the ORC complex at the origin of replication / Swr1 complex / HDACs deacetylate histones / protein targeting to vacuole / Ino80 complex / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / replication fork protection complex / Oxidative Stress Induced Senescence / RMTs methylate histone arginines / postreplication repair / recombinational repair / ATP-dependent chromatin remodeler activity / SUMOylation of chromatin organization proteins / box C/D snoRNP assembly / NuA4 histone acetyltransferase complex / positive regulation of transcription by RNA polymerase I / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / 3'-5' DNA helicase activity / rRNA transcription / Estrogen-dependent gene expression / Ub-specific processing proteases / CENP-A containing nucleosome / heterochromatin organization / nucleosome binding / DNA helicase activity / nucleosomal DNA binding / nuclear periphery / helicase activity / structural constituent of chromatin / rRNA processing / nucleosome / nucleosome assembly / chromatin organization / 5'-3' DNA helicase activity / histone binding / DNA helicase / molecular adaptor activity / protein stabilization / chromatin remodeling / protein heterodimerization activity / DNA repair / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / structural molecule activity / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / DNA binding / ATP binding / nucleus / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能 Vps71/ZNHIT1 / Zinc finger HIT-type profile. / Zinc finger, HIT-type / domain in helicases and associated with SANT domains / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain ... Vps71/ZNHIT1 / Zinc finger HIT-type profile. / Zinc finger, HIT-type / domain in helicases and associated with SANT domains / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / HSA domain / Helicase/SANT-associated domain / HSA domain profile. / : / SNF2-like, N-terminal domain superfamily / SNF2, N-terminal / SNF2-related domain / Actin / Actin family / Actin / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A conserved site / Histone H2A signature. / Histone H2A, C-terminal domain / C-terminus of histone H2A / Histone H2A / Histone 2A / Histone H4, conserved site / Histone H4 signature. / Histone H4 / Histone H4 / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / Histone H3 signature 1. / Helicase conserved C-terminal domain / Histone H3 signature 2. / Histone H3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / ATPase, nucleotide binding domain / Histone-fold / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 Histone H2B.1 / Histone H4 / Histone H2A.1 / Histone H3 / Vacuolar protein sorting-associated protein 71 / RuvB-like protein 1 / Helicase SWR1 / RuvB-like protein 2 / Actin-like protein ARP6 類似検索 - 構成要素生物種 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) / synthetic construct (人工物) 手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.6 Å 詳細 データ登録者Willhoft O / Chua EYD 資金援助 英国, 5件 詳細 詳細を隠すOrganization Grant number 国 Wellcome Trust 095519/Z/11/Z 英国 Wellcome Trust 209327/Z/17/Z 英国 Cancer Research UK C6913/A21608 英国 Medical Research Council (United Kingdom) MR/N009258/1 英国 Medical Research Council (United Kingdom) MR/R009023/1 英国
引用ジャーナル : Science / 年 : 2018タイトル : Structure and dynamics of the yeast SWR1-nucleosome complex.著者 : Oliver Willhoft / Mohamed Ghoneim / Chia-Liang Lin / Eugene Y D Chua / Martin Wilkinson / Yuriy Chaban / Rafael Ayala / Elizabeth A McCormack / Lorraine Ocloo / David S Rueda / Dale B Wigley / 要旨 : The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution ... The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution reveals details of the intricate interactions between components of the SWR1 complex and its nucleosome substrate. Interactions between the Swr1 motor domains and the DNA wrap at superhelical location 2 distort the DNA, causing a bulge with concomitant translocation of the DNA by one base pair, coupled to conformational changes of the histone core. Furthermore, partial unwrapping of the DNA from the histone core takes place upon binding of nucleosomes to SWR1 complex. The unwrapping, as monitored by single-molecule data, is stabilized and has its dynamics altered by adenosine triphosphate binding but does not require hydrolysis. 履歴 登録 2018年4月27日 - ヘッダ(付随情報) 公開 2018年5月30日 - マップ公開 2018年10月17日 - 更新 2024年5月15日 - 現状 2024年5月15日 処理サイト : PDBe / 状態 : 公開
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