+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-4304 | |||||||||
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タイトル | Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhardtii) | |||||||||
マップデータ | Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhartdii) | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 smoothened signaling pathway involved in dorsal/ventral neural tube patterning / myotome development / protein localization to non-motile cilium / regulation of intraciliary retrograde transport / forebrain dorsal/ventral pattern formation / ear morphogenesis / intraciliary transport particle A / intraciliary anterograde transport / cone photoreceptor outer segment / negative regulation of eating behavior ...smoothened signaling pathway involved in dorsal/ventral neural tube patterning / myotome development / protein localization to non-motile cilium / regulation of intraciliary retrograde transport / forebrain dorsal/ventral pattern formation / ear morphogenesis / intraciliary transport particle A / intraciliary anterograde transport / cone photoreceptor outer segment / negative regulation of eating behavior / digestive system development / embryonic heart tube left/right pattern formation / embryonic body morphogenesis / neural tube patterning / photoreceptor cell outer segment organization / intraciliary retrograde transport / protein localization to ciliary membrane / cerebellar Purkinje cell differentiation / intraciliary transport / establishment of protein localization to organelle / embryonic camera-type eye development / gonad development / regulation of cilium assembly / spinal cord dorsal/ventral patterning / photoreceptor connecting cilium / ciliary tip / ventricular system development / Intraflagellar transport / camera-type eye morphogenesis / embryonic brain development / protein localization to cilium / non-motile cilium assembly / regulation of smoothened signaling pathway / embryonic heart tube development / non-motile cilium / embryonic cranial skeleton morphogenesis / nervous system process / 繊毛 / embryonic forelimb morphogenesis / determination of left/right symmetry / embryonic limb morphogenesis / limb development / smoothened signaling pathway / embryonic digit morphogenesis / receptor clustering / axoneme / Bergmann glial cell differentiation / centriolar satellite / cilium assembly / photoreceptor outer segment / Hedgehog 'off' state / negative regulation of smoothened signaling pathway / 中心小体 / ciliary basal body / cellular response to leukemia inhibitory factor / neural tube closure / cell morphogenesis / 繊毛 / microtubule cytoskeleton / positive regulation of canonical Wnt signaling pathway / heart development / protein-containing complex assembly / in utero embryonic development / 細胞骨格 / intracellular signal transduction / 中心体 / chromatin binding / positive regulation of gene expression / regulation of transcription by RNA polymerase II / 生体膜 / 細胞膜 / 細胞質 類似検索 - 分子機能 | |||||||||
生物種 | Chlamydomonas reinhardtii (クラミドモナス) | |||||||||
手法 | サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度: 33.0 Å | |||||||||
データ登録者 | Pigino G / Jordan MA | |||||||||
引用 | ジャーナル: Nat Cell Biol / 年: 2018 タイトル: The cryo-EM structure of intraflagellar transport trains reveals how dynein is inactivated to ensure unidirectional anterograde movement in cilia. 著者: Mareike A Jordan / Dennis R Diener / Ludek Stepanek / Gaia Pigino / 要旨: Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components along the microtubules to and from the ...Movement of cargos along microtubules plays key roles in diverse cellular processes, from signalling to mitosis. In cilia, rapid movement of ciliary components along the microtubules to and from the assembly site is essential for the assembly and disassembly of the structure itself. This bidirectional transport, known as intraflagellar transport (IFT), is driven by the anterograde motor kinesin-2 and the retrograde motor dynein-1b (dynein-2 in mammals). However, to drive retrograde transport, dynein-1b must first be delivered to the ciliary tip by anterograde IFT. Although, the presence of opposing motors in bidirectional transport processes often leads to periodic stalling and slowing of cargos, IFT is highly processive. Using cryo-electron tomography, we show that a tug-of-war between kinesin-2 and dynein-1b is prevented by loading dynein-1b onto anterograde IFT trains in an autoinhibited form and by positioning it away from the microtubule track to prevent binding. Once at the ciliary tip, dynein-1b must transition into an active form and engage microtubules to power retrograde trains. These findings provide a striking example of how coordinated structural changes mediate the behaviour of complex cellular machinery. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_4304.map.gz | 30.1 KB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-4304-v30.xml emd-4304.xml | 8.4 KB 8.4 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_4304_1.png emd_4304_2.png emd_4304_3.png | 142.5 KB 95.8 KB 85.2 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-4304 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4304 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_4304.map.gz / 形式: CCP4 / 大きさ: 48.8 KB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhartdii) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 14.13 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : Intraflagellar transport complex A
全体 | 名称: Intraflagellar transport complex A |
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要素 |
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-超分子 #1: Intraflagellar transport complex A
超分子 | 名称: Intraflagellar transport complex A / タイプ: complex / ID: 1 / 親要素: 0 |
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由来(天然) | 生物種: Chlamydomonas reinhardtii (クラミドモナス) 株: CC-124 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | サブトモグラム平均法 |
試料の集合状態 | 2D array |
-試料調製
緩衝液 | pH: 7 |
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グリッド | モデル: Quantifoil R3.5/1 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 前処理 - タイプ: GLOW DISCHARGE |
凍結 | 凍結剤: ETHANE / 装置: LEICA EM GP |
-電子顕微鏡法
顕微鏡 | FEI TITAN |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
特殊光学系 | エネルギーフィルター - 名称: GIF Quantum LS |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 2.1 e/Å2 |
-画像解析
抽出 | トモグラム数: 15 / 使用した粒子像数: 750 |
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CTF補正 | ソフトウェア - 名称: CTFPHASEFLIP |
最終 角度割当 | タイプ: NOT APPLICABLE |
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 33.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: PEET (ver. 1.11.0) / 使用したサブトモグラム数: 1450 |