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データを開く
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基本情報
登録情報 | ![]() | ||||||||||||
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タイトル | Subtomogram averaged consensus structure of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes | ||||||||||||
![]() | In situ consensus reconstruction of Plasmodium falciparum parasite Pf80S ribosome | ||||||||||||
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![]() | ribosome / malaria / cryoET / in situ / Plasmodium falciparum / translation | ||||||||||||
機能・相同性 | ![]() RMTs methylate histone arginines / Major pathway of rRNA processing in the nucleolus and cytosol / Protein methylation / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Translesion synthesis by POLK / Translesion synthesis by POLI / Josephin domain DUBs / Metalloprotease DUBs ...RMTs methylate histone arginines / Major pathway of rRNA processing in the nucleolus and cytosol / Protein methylation / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Translesion synthesis by POLK / Translesion synthesis by POLI / Josephin domain DUBs / Metalloprotease DUBs / DNA Damage Recognition in GG-NER / Formation of Incision Complex in GG-NER / Dual Incision in GG-NER / Formation of TC-NER Pre-Incision Complex / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / ER Quality Control Compartment (ERQC) / Iron uptake and transport / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Translation initiation complex formation / Formation of a pool of free 40S subunits / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / GTP hydrolysis and joining of the 60S ribosomal subunit / Negative regulators of DDX58/IFIH1 signaling / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Aggrephagy / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Orc1 removal from chromatin / CDK-mediated phosphorylation and removal of Cdc6 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / KEAP1-NFE2L2 pathway / UCH proteinases / Ub-specific processing proteases / Neddylation / MAPK6/MAPK4 signaling / Antigen processing: Ubiquitination & Proteasome degradation / ABC-family proteins mediated transport / AUF1 (hnRNP D0) binds and destabilizes mRNA / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / positive regulation of apoptotic signaling pathway / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / rRNA processing / protein tag activity / large ribosomal subunit / ribosome binding / kinase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / ubiquitin-dependent protein catabolic process / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / mRNA binding / ubiquitin protein ligase binding / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度: 4.1 Å | ||||||||||||
![]() | Anton L / Cheng W / Zhu X / Ho CM | ||||||||||||
資金援助 | ![]() ![]() ![]()
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![]() | ![]() タイトル: Divergent translational landscape reflects adaptation to biased codon usage in malaria parasites 著者: Anton L / Cheng W / Haile M / Cobb DW / Zhu X / Han L / Li E / Nair A / Lee CL / Ho CM | ||||||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | |||
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ヘッダ (付随情報) | ![]() | |||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8tpuMC ![]() 41486 ![]() 41487 ![]() 41488 ![]() 41489 ![]() 41490 ![]() 41491 ![]() 41492 ![]() 41493 ![]() 41494 M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | In situ consensus reconstruction of Plasmodium falciparum parasite Pf80S ribosome | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.69 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Plasmodium falciparum 80S ribosome consensus structure
+超分子 #1: Plasmodium falciparum 80S ribosome consensus structure
+分子 #1: 40S ribosomal protein S24
+分子 #2: 40S ribosomal protein S25
+分子 #3: 40S ribosomal protein S26
+分子 #4: 40S ribosomal protein S27
+分子 #5: 60S ribosomal protein L41
+分子 #6: Small ribosomal subunit protein eS30
+分子 #9: 40S ribosomal protein S3a
+分子 #10: 40S ribosomal protein SA
+分子 #11: 40S ribosomal protein S3
+分子 #12: 40S ribosomal protein S9, putative
+分子 #13: 40S ribosomal protein S4
+分子 #14: 40S ribosomal protein S5
+分子 #15: 40S ribosomal protein S6
+分子 #16: 40S ribosomal protein S5, putative
+分子 #17: 40S ribosomal protein S7
+分子 #18: 40S ribosomal protein S15A, putative
+分子 #19: 40S ribosomal protein S8
+分子 #20: 40S ribosomal protein S16
+分子 #21: 40S ribosomal protein S20e, putative
+分子 #22: 40S ribosomal protein S10, putative
+分子 #23: 40S ribosomal protein S11
+分子 #24: 40S ribosomal protein S23, putative
+分子 #25: 40S ribosomal protein S12
+分子 #26: 40S ribosomal protein S18, putative
+分子 #27: 40S ribosomal protein S29, putative
+分子 #28: 40S ribosomal protein S15
+分子 #29: 40S ribosomal protein S11, putative
+分子 #30: 40S ribosomal protein S17, putative
+分子 #31: 40S ribosomal protein S19
+分子 #32: 40S ribosomal protein S19
+分子 #33: 40S ribosomal protein S21
+分子 #37: 60S ribosomal protein L13
+分子 #38: 60S ribosomal protein L27
+分子 #39: 60S ribosomal protein L28
+分子 #40: 60S ribosomal protein L29
+分子 #41: 60S ribosomal protein L30e, putative
+分子 #42: 60S ribosomal protein L31
+分子 #43: 60S ribosomal protein L14, putative
+分子 #44: 60S ribosomal protein L32
+分子 #45: 60S ribosomal protein L35ae, putative
+分子 #46: 60S ribosomal protein L34
+分子 #47: 60S ribosomal protein L36
+分子 #48: 60S ribosomal protein L38
+分子 #49: 60S ribosomal protein L39
+分子 #50: Ubiquitin-60S ribosomal protein L40
+分子 #51: Ribosomal protein L15
+分子 #52: Large ribosomal subunit protein eL43
+分子 #53: Large ribosomal subunit protein eL42
+分子 #54: 60S ribosomal protein L6, putative
+分子 #55: 60S ribosomal protein L7a
+分子 #56: Ribosomal protein L37
+分子 #57: 60S ribosomal protein L13, putative
+分子 #58: 60S ribosomal protein L23, putative
+分子 #59: 60S ribosomal protein L18-2, putative
+分子 #60: 60S ribosomal protein L27a, putative
+分子 #61: 60S ribosomal protein L10, putative
+分子 #62: 60S ribosomal protein L5, putative
+分子 #63: 60S ribosomal protein L17, putative
+分子 #64: 60S ribosomal protein L23
+分子 #65: 60S ribosomal protein L19
+分子 #66: 60S ribosomal protein L26, putative
+分子 #67: 60S ribosomal protein L35, putative
+分子 #68: 60S ribosomal protein L7, putative
+分子 #69: 60S ribosomal protein L2
+分子 #70: 60S ribosomal protein L3
+分子 #71: 60S ribosomal protein L4
+分子 #72: 60S ribosomal protein L11a, putative
+分子 #73: 60S ribosomal protein L18a
+分子 #74: 60S ribosomal protein L6, putative
+分子 #75: 60S ribosomal protein L21
+分子 #76: 60S ribosomal protein L41
+分子 #77: 60S ribosomal protein L22, putative
+分子 #78: 60S ribosomal protein L24, putative
+分子 #79: Receptor for activated c kinase
+分子 #7: 60S ribosomal protein L24, putative
+分子 #8: 18S ribosomal RNA
+分子 #34: 28S ribosomal RNA
+分子 #35: 5.8S ribosomal RNA
+分子 #36: 5S ribosomal RNA
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | サブトモグラム平均法 |
試料の集合状態 | cell |
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試料調製
緩衝液 | pH: 7.2 |
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凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 2.93 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.2 µm 最小 デフォーカス(公称値): 2.8000000000000003 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 4.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用したサブトモグラム数: 120226 |
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抽出 | トモグラム数: 848 / 使用した粒子像数: 129617 |
最終 角度割当 | タイプ: OTHER |