+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-4140 | |||||||||
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タイトル | Nonstop ribosomal complex bound with Dom34 and Hbs1 | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | mRNA surveillance / ribosome | |||||||||
機能・相同性 | 機能・相同性情報 Eukaryotic Translation Elongation / Dom34-Hbs1 complex / RNA surveillance / nuclear-transcribed mRNA catabolic process, no-go decay / nuclear-transcribed mRNA catabolic process, non-stop decay / HSF1 activation / nonfunctional rRNA decay / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting ...Eukaryotic Translation Elongation / Dom34-Hbs1 complex / RNA surveillance / nuclear-transcribed mRNA catabolic process, no-go decay / nuclear-transcribed mRNA catabolic process, non-stop decay / HSF1 activation / nonfunctional rRNA decay / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / positive regulation of translational fidelity / ribosome disassembly / Protein methylation / RMTs methylate histone arginines / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / preribosome, large subunit precursor / ribosomal large subunit export from nucleus / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / G-protein alpha-subunit binding / positive regulation of protein kinase activity / positive regulation of translational initiation / regulation of translational fidelity / protein-RNA complex assembly / Ub-specific processing proteases / ribosomal small subunit export from nucleus / translation regulator activity / translation elongation factor activity / ribosomal subunit export from nucleus / translational termination / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / Neutrophil degranulation / RNA endonuclease activity / cellular response to amino acid starvation / ribosome assembly / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / meiotic cell cycle / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / positive regulation of translation / macroautophagy / small-subunit processome / positive regulation of apoptotic signaling pathway / protein kinase C binding / maintenance of translational fidelity / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / ribosomal large subunit assembly / modification-dependent protein catabolic process / cytoplasmic stress granule / rRNA processing / protein tag activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / translation / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / cell division / negative regulation of gene expression / response to antibiotic / GTPase activity / mRNA binding 類似検索 - 分子機能 | |||||||||
生物種 | Saccharomyces cerevisiae (パン酵母) / synthetic construct (人工物) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | |||||||||
データ登録者 | Hilal T / Yamamoto H | |||||||||
資金援助 | ドイツ, 2件
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引用 | ジャーナル: Nat Commun / 年: 2016 タイトル: Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution. 著者: Tarek Hilal / Hiroshi Yamamoto / Justus Loerke / Jörg Bürger / Thorsten Mielke / Christian M T Spahn / 要旨: The surveillance of mRNA translation is imperative for homeostasis. Monitoring the integrity of the message is essential, as the translation of aberrant mRNAs leads to stalling of the translational ...The surveillance of mRNA translation is imperative for homeostasis. Monitoring the integrity of the message is essential, as the translation of aberrant mRNAs leads to stalling of the translational machinery. During ribosomal rescue, arrested ribosomes are specifically recognized by the conserved eukaryotic proteins Dom34 and Hbs1, to initiate their recycling. Here we solve the structure of Dom34 and Hbs1 bound to a yeast ribosome programmed with a nonstop mRNA at 3.3 Å resolution using cryo-electron microscopy. The structure shows that Domain N of Dom34 is inserted into the upstream mRNA-binding groove via direct stacking interactions with conserved nucleotides of 18S rRNA. It senses the absence of mRNA at the A-site and part of the mRNA entry channel by direct competition. Thus, our analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34·Hbs1·GTP complex during Dom34-mediated mRNA surveillance pathways. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_4140.map.gz | 143.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-4140-v30.xml emd-4140.xml | 99 KB 99 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_4140_fsc.xml | 17.1 KB | 表示 | FSCデータファイル |
画像 | emd_4140.png | 111.2 KB | ||
Filedesc metadata | emd-4140.cif.gz | 19.7 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-4140 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4140 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_4140_validation.pdf.gz | 298 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_4140_full_validation.pdf.gz | 297.1 KB | 表示 | |
XML形式データ | emd_4140_validation.xml.gz | 14.6 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4140 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4140 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_4140.map.gz / 形式: CCP4 / 大きさ: 266.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 0.994 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : Nonstop ribosomal complex bound with Dom34-Hbs1-GMPPNP
+超分子 #1: Nonstop ribosomal complex bound with Dom34-Hbs1-GMPPNP
+分子 #1: Protein DOM34
+分子 #2: 40S ribosomal protein S0-A
+分子 #3: 40S ribosomal protein S26-B
+分子 #4: 40S ribosomal protein S1-A
+分子 #5: 40S ribosomal protein S27-A
+分子 #6: 40S ribosomal protein S2
+分子 #7: 40S ribosomal protein S28-B
+分子 #8: 40S ribosomal protein S3
+分子 #9: 40S ribosomal protein S29-A
+分子 #10: 40S ribosomal protein S4-A
+分子 #11: 40S ribosomal protein S30-A
+分子 #12: 40S ribosomal protein S5
+分子 #13: 40S ribosomal protein S6-A
+分子 #14: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #15: 40S ribosomal protein S7-A
+分子 #16: 40S ribosomal protein S8-A
+分子 #17: 40S ribosomal protein S9-A
+分子 #18: 40S ribosomal protein S10-A
+分子 #19: 40S ribosomal protein S11-A
+分子 #20: 40S ribosomal protein S12
+分子 #21: 40S ribosomal protein S13
+分子 #22: 40S ribosomal protein S14-A
+分子 #23: 40S ribosomal protein S15
+分子 #24: 40S ribosomal protein S16-A
+分子 #25: 40S ribosomal protein S17-B
+分子 #26: 40S ribosomal protein S18-A
+分子 #27: 40S ribosomal protein S19-A
+分子 #28: 40S ribosomal protein S20
+分子 #29: 40S ribosomal protein S21-A
+分子 #30: 40S ribosomal protein S22-A
+分子 #31: 40S ribosomal protein S23-A
+分子 #32: 40S ribosomal protein S24-A
+分子 #33: 40S ribosomal protein S25-A
+分子 #35: Ubiquitin-40S ribosomal protein S31
+分子 #39: 60S ribosomal protein L28
+分子 #40: 60S ribosomal protein L2-A
+分子 #41: 60S ribosomal protein L29
+分子 #42: 60S ribosomal protein L3
+分子 #43: 60S ribosomal protein L30
+分子 #44: 60S ribosomal protein L4-A
+分子 #45: 60S ribosomal protein L5
+分子 #46: 60S ribosomal protein L31-A
+分子 #47: 60S ribosomal protein L32
+分子 #48: 60S ribosomal protein L33-A
+分子 #49: 60S ribosomal protein L7-A
+分子 #50: 60S ribosomal protein L34-A
+分子 #51: 60S ribosomal protein L8-A
+分子 #52: 60S ribosomal protein L35-A
+分子 #53: 60S ribosomal protein L9-A
+分子 #54: 60S ribosomal protein L36-A
+分子 #55: 60S ribosomal protein L10
+分子 #56: 60S ribosomal protein L11-A
+分子 #57: 60S ribosomal protein L37-A
+分子 #58: 60S ribosomal protein L38
+分子 #59: 60S ribosomal protein L39
+分子 #60: 60S ribosomal protein L13-A
+分子 #61: Ubiquitin-60S ribosomal protein L40
+分子 #62: 60S ribosomal protein L14-A
+分子 #63: 60S ribosomal protein L15-A
+分子 #64: 60S ribosomal protein L42-B
+分子 #65: 60S ribosomal protein L43-B
+分子 #66: 60S ribosomal protein L17-A
+分子 #67: 60S ribosomal protein L18-A
+分子 #68: 60S ribosomal protein L20-A
+分子 #69: 60S ribosomal protein L22-A
+分子 #70: 60S ribosomal protein L23-A
+分子 #71: 60S ribosomal protein L27-A
+分子 #72: 60S ribosomal protein L16-A
+分子 #73: 60S ribosomal protein L41-B
+分子 #74: 60S ribosomal protein L19-A
+分子 #75: 60S ribosomal protein L25
+分子 #76: 60S ribosomal protein L26-A
+分子 #77: 60S ribosomal protein L21-A
+分子 #78: 60S ribosomal protein L6-A
+分子 #79: 60S ribosomal protein L24-A
+分子 #80: Protein HBS1
+分子 #34: 18S ribosomal RNA
+分子 #36: 25S ribosomal RNA
+分子 #37: 5S ribosomal RNA
+分子 #38: 5.8S ribosomal RNA
+分子 #81: nonstop mRNA
+分子 #82: yeast Phe-tRNA-Phe
+分子 #83: ZINC ION
+分子 #84: MAGNESIUM ION
+分子 #85: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
+分子 #86: N-acetyl-L-phenylalanine
+分子 #87: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI POLARA 300 |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / デジタル化 - 画像ごとのフレーム数: 1-25 / 実像数: 4797 / 平均露光時間: 5.0 sec. / 平均電子線量: 25.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.0 mm / 最大 デフォーカス(公称値): 4.5 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 80645 |
試料ステージ | ホルダー冷却材: NITROGEN |
実験機器 | モデル: Tecnai Polara / 画像提供: FEI Company |
+画像解析
-原子モデル構築 1
精密化 | 空間: REAL / プロトコル: FLEXIBLE FIT |
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得られたモデル | PDB-5m1j: |