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- EMDB-21859: CryoEM structure of yeast 80S ribosome with Met-tRNAiMet, eIF5B, ... -

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Basic information

Entry
Database: EMDB / ID: EMD-21859
TitleCryoEM structure of yeast 80S ribosome with Met-tRNAiMet, eIF5B, and GDP
Map data
Sample80S ribosome with eIF5B, Met-tRNAiMet, mRNA, and GDP:
(nucleic-acidNucleic acid) x 6 / uL2 / uL3 / uL4 / uL18 / eL6 / uL30 / eL8 / uL6 / uL16 ...(nucleic-acidNucleic acid) x 6 / uL2 / uL3 / uL4 / uL18 / eL6 / uL30 / eL8 / uL6 / uL16 / uL5 / eL13 / eL14 / eL15List of Subaru engines / uL13 / uL22 / eL18 / eL19 / eL20 / eL21 / eL22 / uL14 / eL24 / uL23 / uL24 / eL27 / uL15 / eL29 / eL30 / eL31 / eL32CD59 / eL33 / eL34 / uL29 / eL36 / eL37 / eL38 / eL39 / eL40 / eL41 / eL42 / eL43 / uL1 / uL10 / L10 / uS2 / eS1 / uS5 / uS3 / eS4 / uS7 / eS6 / eS7 / eS8 / uS4 / eS10 / uS17 / eS12 / uS15 / uS11 / (uS19) x 2 / eS17 / uS13 / eS19 / uS10 / eS21 / uS8 / uS12 / eS24 / eS25 / eS26 / eS27 / eS28 / uS14 / eS30 / eS31 / RACK1Receptor for activated C kinase 1 / eIF5B / (ligand) x 4
Function / homology
Function and homology information


protein-synthesizing GTPase / eukaryotic 48S preinitiation complex / formation of cytoplasmic translation initiation complex / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / regulation of cellular response to stress / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / regulation of translational initiation / invasive growth in response to glucose limitation / GDP-dissociation inhibitor activity ...protein-synthesizing GTPase / eukaryotic 48S preinitiation complex / formation of cytoplasmic translation initiation complex / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / regulation of cellular response to stress / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / regulation of translational initiation / invasive growth in response to glucose limitation / GDP-dissociation inhibitor activity / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / pre-mRNA 5'-splice site binding / rRNA export from nucleus / preribosome, small subunit precursor / preribosome, large subunit precursor / mRNA destabilization / response to cycloheximide / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / rescue of stalled ribosome / ribonucleoprotein complex assembly / rRNA methylation / negative regulation of mRNA splicing, via spliceosome / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit export from nucleus / ribosomal small subunit export from nucleus / maturation of SSU-rRNA / regulation of translational fidelity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / small-subunit processome / ribosomal large subunit biogenesis / ribosomal small subunit biogenesis / protein kinase A catalytic subunit binding / translation initiation factor binding / cellular response to amino acid starvation / positive regulation of protein kinase activity / mature ribosome assembly / maturation of LSU-rRNA / translational termination / translation initiation factor activity / ribosome assembly / DNA-(apurinic or apyrimidinic site) endonuclease activity / assembly of large subunit precursor of preribosome / positive regulation of translational fidelity / maintenance of translational fidelity / protein kinase C binding / protein localization / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / modification-dependent protein catabolic process / macroautophagy / mRNA 5'-UTR binding / protein tag / rRNA processing / large ribosomal subunit rRNA binding / cytoplasmic stress granule / cytoplasmic translation / ribosome biogenesis / cytosolic large ribosomal subunit / ribosome binding / 5S rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit / negative regulation of translation / ribosome / rRNA binding / structural constituent of ribosome / protein ubiquitination / translation / GTPase activity / negative regulation of gene expression / positive regulation of protein phosphorylation / mRNA binding / G protein-coupled receptor signaling pathway / GTP binding / nucleolus / ubiquitin protein ligase binding / enzyme binding / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm
Ribosomal protein L34e, conserved site / Ribosomal protein S12/S23 / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L13, eukaryotic/archaeal ...Ribosomal protein L34e, conserved site / Ribosomal protein S12/S23 / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L13 / KOW / Ribosomal protein L24/L26, conserved site / Ribosomal protein L7, eukaryotic / Ribosomal protein S30 / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein L34Ae / Translation protein SH3-like domain superfamily / Translation protein, beta-barrel domain superfamily / K homology domain superfamily, prokaryotic type / S15/NS1, RNA-binding / Ribosomal protein S13-like, H2TH / Ribosomal protein L37ae/L37e / Zinc-binding ribosomal protein / Nucleic acid-binding, OB-fold / Ribosomal protein S13/S15, N-terminal / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L23/L15e core domain superfamily / Ribosomal protein L30, N-terminal / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L23/L25, N-terminal / Ribosomal protein S5, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal protein L29/L35 / Ribosomal protein S2 / Ribosomal protein S13 / Ribosomal protein S4/S9, N-terminal / Ribosomal protein L7A/L8 / Ribosomal protein S21e / Ribosomal protein S11 / Ribosomal protein L40e / Ribosomal protein S24e / Ribosomal protein L5 / Ribosomal protein L4/L1e / Ribosomal protein L1 / Ribosomal protein L2 / Ribosomal protein S19/S15 / Ribosomal protein L22e / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal protein L29e / Ribosomal protein L37ae / Ribosomal protein L38e / Ribosomal protein L14e domain / Ribosomal protein S27a / RNA-binding S4 domain / Ribosomal protein L7Ae conserved site / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / K Homology domain, type 2 / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S25 / Small GTP-binding protein domain / Ribosomal protein S5, C-terminal / Plectin/S10, N-terminal / Ribosomal protein L25/L23 / Ribosomal protein S4e, N-terminal / Ribosomal protein L10P / Ribosomal protein L31e, conserved site / Ribosomal protein S21e, conserved site / Ribosomal protein S3, conserved site / Ribosomal protein S3Ae, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S8e, conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / WD40 repeat, conserved site / Ribosomal protein L3, conserved site / Ubiquitin conserved site / Ubiquitin domain / Ribosomal protein L14P, conserved site / Ribosomal protein S17, conserved site / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L39e, conserved site / Ribosomal protein S17e, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Ribosomal protein L15e, conserved site / Ribosomal protein L5, conserved site / Ribosomal protein S19 conserved site / Ribosomal protein L18e/L15P / Ribosomal protein L18e, conserved site / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S14, conserved site
40S ribosomal protein S2 / Guanine nucleotide-binding protein subunit beta-like protein / 60S ribosomal protein L14-A / 40S ribosomal protein S27-A / 40S ribosomal protein S7-A / 60S ribosomal protein L16-A / 60S ribosomal protein L5 / 60S ribosomal protein L30 / 40S ribosomal protein S1-B / 60S ribosomal protein L8-A ...40S ribosomal protein S2 / Guanine nucleotide-binding protein subunit beta-like protein / 60S ribosomal protein L14-A / 40S ribosomal protein S27-A / 40S ribosomal protein S7-A / 60S ribosomal protein L16-A / 60S ribosomal protein L5 / 60S ribosomal protein L30 / 40S ribosomal protein S1-B / 60S ribosomal protein L8-A / 40S ribosomal protein S17-B / 60S ribosomal protein L3 / 60S ribosomal protein L4-A / 60S ribosomal protein L35-A / 60S ribosomal protein L19-A / 40S ribosomal protein S20 / 60S ribosomal protein L32 / 40S ribosomal protein S15 / 40S ribosomal protein S14-B / 60S ribosomal protein L34-A / 40S ribosomal protein S25-A / 60S ribosomal protein L11-B / 60S ribosomal protein L13-A / 40S ribosomal protein S10-A / 60S ribosomal protein L21-A / 60S ribosomal protein L6-A / 40S ribosomal protein S16-A / 60S ribosomal protein L9-B / Eukaryotic translation initiation factor 5B / 60S ribosomal protein L38 / 60S ribosomal protein L37-A / 40S ribosomal protein S12 / 40S ribosomal protein S0-B / 60S ribosomal protein L10 / 40S ribosomal protein S29-A / 40S ribosomal protein S26-B / 40S ribosomal protein S18-A / 60S ribosomal protein L42-A / 60S ribosomal protein L18-A / 60S ribosomal protein L26-A / 40S ribosomal protein S3 / 60S ribosomal protein L22-A / 60S ribosomal protein L15-A / 60S ribosomal protein L29 / 60S ribosomal protein L36-A / 60S ribosomal protein L33-A / 60S ribosomal protein L17-A / 40S ribosomal protein S13 / 60S ribosomal protein L7-A / 60S acidic ribosomal protein P0 / 60S ribosomal protein L39 / 60S ribosomal protein L25 / 60S ribosomal protein L24-A / 60S ribosomal protein L28 / RPS5 isoform 1 / 40S ribosomal protein S9-B / Ubiquitin-40S ribosomal protein S31 / 40S ribosomal protein S11-A / 40S ribosomal protein S24-A / 60S ribosomal protein L2-A / 60S ribosomal protein L1-A / 60S ribosomal protein L23-A / 40S ribosomal protein S8-A / 40S ribosomal protein S6-A / 40S ribosomal protein S4-A / 40S ribosomal protein S30-A / 40S ribosomal protein S23-A / 40S ribosomal protein S19-A / 60S ribosomal protein L43-A / 60S ribosomal protein L20-A / Ubiquitin-60S ribosomal protein L40 / 60S ribosomal protein L31-A / 60S ribosomal protein L27-A / 40S ribosomal protein S22-A / 40S ribosomal protein S21-A / 40S ribosomal protein S28-A
Biological speciesSaccharomyces cerevisiae (baker's yeast) / Baker's yeast (baker's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsWang J / Wang J / Puglisi J / Fernandez IS
CitationJournal: Nat Commun / Year: 2020
Title: Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex.
Authors: Jinfan Wang / Jing Wang / Byung-Sik Shin / Joo-Ran Kim / Thomas E Dever / Joseph D Puglisi / Israel S Fernández /
Abstract: Recognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the ...Recognition of a start codon by the initiator aminoacyl-tRNA determines the reading frame of messenger RNA (mRNA) translation by the ribosome. In eukaryotes, the GTPase eIF5B collaborates in the correct positioning of the initiator Met-tRNA on the ribosome in the later stages of translation initiation, gating entrance into elongation. Leveraging the long residence time of eIF5B on the ribosome recently identified by single-molecule fluorescence measurements, we determine the cryoEM structure of the naturally long-lived ribosome complex with eIF5B and Met-tRNA immediately before transition into elongation. The structure uncovers an unexpected, eukaryotic specific and dynamic fidelity checkpoint implemented by eIF5B in concert with components of the large ribosomal subunit.
Validation ReportPDB-ID: 6woo

SummaryFull reportAbout validation report
History
DepositionApr 25, 2020-
Header (metadata) releaseSep 23, 2020-
Map releaseSep 23, 2020-
UpdateSep 23, 2020-
Current statusSep 23, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6woo
  • Surface level: 0.042
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_21859.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.42 Å/pix.
x 300 pix.
= 424.8 Å
1.42 Å/pix.
x 300 pix.
= 424.8 Å
1.42 Å/pix.
x 300 pix.
= 424.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.416 Å
Density
Contour LevelBy AUTHOR: 0.042 / Movie #1: 0.042
Minimum - Maximum-0.09174424 - 0.2578786
Average (Standard dev.)0.0017547369 (±0.010322071)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 424.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.4161.4161.416
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z424.800424.800424.800
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ400400400
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.0920.2580.002

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Supplemental data

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Segmentation: #1

Fileemd_21859_msk_1.map
Projections & Slices
AxesZYX

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Additional map: unsharpened map

Fileemd_21859_additional_1.map
Annotationunsharpened map
Projections & Slices
AxesZYX

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Additional map: post-processed map, with Bfactor -50 and low-pass filtered to 3.3A

Fileemd_21859_additional_2.map
Annotationpost-processed map, with Bfactor -50 and low-pass filtered to 3.3A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: half-1

Fileemd_21859_half_map_1.map
Annotationhalf-1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: half-map 2

Fileemd_21859_half_map_2.map
Annotationhalf-map 2
Projections & Slices
AxesZYX

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Sample components

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Entire 80S ribosome with eIF5B, Met-tRNAiMet, mRNA, and GDP

EntireName: 80S ribosome with eIF5B, Met-tRNAiMet, mRNA, and GDP / Number of components: 89

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Component #1: protein, 80S ribosome with eIF5B, Met-tRNAiMet, mRNA, and GDP

ProteinName: 80S ribosome with eIF5B, Met-tRNAiMet, mRNA, and GDP / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

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Component #2: nucleic-acid, 25S ribosomal RNA

nucleic acidName: 25S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAAC GGCGAGUGAA GCGGCAAAAG CUCAAAUUUG AAAUCUGGUA CCUUCGGUGC CCGAGUUGUA AUUUGGAGAG GGCAACUUUG ...Sequence:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAAC GGCGAGUGAA GCGGCAAAAG CUCAAAUUUG AAAUCUGGUA CCUUCGGUGC CCGAGUUGUA AUUUGGAGAG GGCAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GCGGUUCUUU GUAAAGUGCC UUCGAAGAGU CGAGUUGUUU GGGAAUGCAG CUCUAAGUGG GUGGUAAAUU CCAUCUAAAG CUAAAUAUUG GCGAGAGACC GAUAGCGAAC AAGUACAGUG AUGGAAAGAU GAAAAGAACU UUGAAAAGAG AGUGAAAAAG UACGUGAAAU UGUUGAAAGG GAAGGGCAUU UGAUCAGACG CCAGCAUCAG UUUUGGUGGC AGGAUAAAUC CAUAGGAAUG UAGCUUGCCU CGGUAAGUAU UAUAGCCUGU GGGAAUACUG CCAGCUGGGA CUGAGGACUG CGACGUAAGU CAAGGAUGCU GGCAUAAUGG UUAUAUGCCG CCCGUCUUGA AACACGGACC AAGGAGUCUA ACGUCUAUGC GAGUGUUUGG GUGUAAAACC CAUACGCGUA AUGAAAGUGA ACGUAGGUUG GGGCCUCGCA AGAGGUGCAC AAUCGACCGA UCCUGAUGUC UUCGGAUGGA UUUGAGUAAG AGCAUAGCUG UUGGGACCCG AAAGAUGGUG AACUAUGCCU GAAUAGGGUG AAGCCAGAGG AAACUCUGGU GGAGGCUCGU AGCGGUUCUG ACGUGCAAAU CGAUCGUCGA AUUUGGGUAU AGGGGCGAAA GACUAAUCGA ACCAUCUAGU AGCUGGUUCC UGCCGAAGUU UCCCUCAGGA UAGCAGAAGC UCGUAUCAGU UUUAUGAGGU AAAGCGAAUG AUUAGAGGUU CCGGGGUCGA AAUGACCUUG ACCUAUUCUC AAACUUUAAA UAUGUAAGAA GUCCUUGUUA CUUAAUUGAA CGUGGACAUU UGAAUGAAGA GCUUUUAGUG GGCCAUUUUU GGUAAGCAGA ACUGGCGAUG CGGGAUGAAC CGAACGUAGA GUUAAGGUGC CGGAAUACAC GCUCAUCAGA CACCACAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCGGCCG AAUGAACUAG CCCUGAAAAU GGAUGGCGCU CAAGCGUGUU ACCUAUACUC UACCGUCAGG GUUGAUAUGA UGCCCUGACG AGUAGGCAGG CGUGGAGGUC AGUGACGAAG CCUAGACCGU AAGGUCGGGU CGAACGGCCU CUAGUGCAGA UCUUGGUGGU AGUAGCAAAU AUUCAAAUGA GAACUUUGAA GACUGAAGUG GGGAAAGGUU CCACGUCAAC AGCAGUUGGA CGUGGGUUAG UCGAUCCUAA GAGAUGGGGA AGCUCCGUUU CAAAGGCCUG AUUUUAUGCA GGCCACCAUC GAAAGGGAAU CCGGUUAAGA UUCCGGAACC UGGAUAUGGA UUCUUCACGG UAACGUAACU GAAUGUGGAG ACGUCGGCGC GAGCCCUGGG AGGAGUUAUC UUUUCUUCUU AACAGCUUAU CACCCCGGAA UUGGUUUAUC CGGAGAUGGG GUCUUAUGGC UGGAAGAGGC CAGCACCUUU GCUGGCUCCG GUGCGCUUGU GACGGCCCGU GAAAAUCCAC AGGAAGGAAU AGUUUUCAUG CCAGGUCGUA CUGAUAACCG CAGCAGGUCU CCAAGGUGAA CAGCCUCUAG UUGAUAGAAU AAUGUAGAUA AGGGAAGUCG GCAAAAUAGA UCCGUAACUU CGGGAUAAGG AUUGGCUCUA AGGGUCGGGU AGUGAGGGCC UUGGUCAGAC GCAGCGUUGU AGACGGCCUU GGUAGGUCUC UUGUAGAUGC UACAAUUACG AUCAACUUAG AACUGGUACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCAUUG CGAUGGUCAG AAAGUGAUGU UGACGCAAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAACCAAGC GCGGGUAAAC GGCGGGAGUA ACUAUGACUC UCUUAAGGUA GCCAAAUGCC UCGUCAUCUA AUUAGUGACG CGCAUGAAUG GAUUAACGAG AUUCCCACUG UCCCUAUCUA CUAUCUAGCG AAACCACAGC CAAGGGAACG GGCUUGGCAG AAUCAGCGGG GAAAGAAGAC CCUGUUGAGC UUGACUCUAG UUUGACAUUG UGAAGAGACA UAGAGGGUGU AGAAUAAGUG GGAGCUUCGG CGCCAGUGAA AUACCACUAC CUUUAUAGUU UCUUUACUUA UUCAAUGAAG CGGAGCUGGA AUUCAUUUUC CACGUUCUAG CAUUCAAGGU CCCAUUCGGG GCUGAUCCGG GUUGAAGACA UUGUCAGGUG GGGAGUUUGG CUGGGGCGGC ACAUCUGUUA AACGAUAACG CAGAUGUCCU AAGGGGGGCU CAUGGAGAAC AGAAAUCUCC AGUAGAACAA AAGGGUAAAA GCCCCCUUGA UUUUGAUUUU CAGUGUGAAU ACAAACCAUG AAAGUGUGGC CUAUCGAUCC UUUAGUCCCU CGGAAUUUGA GGCUAGAGGU GCCAGAAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCUCUUCC UAUCAUACCG AAGCAGAAUU CGGUAAGCGU UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGAA UGUUACCGCA AUAGUAAUUG AACUUAGUAC GAGAGGAACA GUUCAUUCGG AUAAUUGGUU UUUGCGGCUG UCUGAUCAGG CAUUGCCGCG AAGCUACCAU CCGCUGGAUU AUGGCUGAAC GCCUCUAAGU CAGAAUCCAU GCUAGAACGC GGUGAUUUCU UUGCUCCACA CAAUAUAGAU GGAUACGAAU AAGGCGUCCU UGUGGCGUCG CUGAACCAUA GCAGGCUAGC AACGGUGCAC UUGGCGGAAA GGCCUUGGGU GCUUGCUGGC GAAUUGCAAU GUCAUUUUGC GUGGGGAUAA AUCAUUUGUA UACGACUUAG AUGUACAACG GGGUAUUGUA AGCAGUAGAG UAGCCUUGUU GUUACGAUCU GCUGAGAUUA AGCCUUUGUU GUCUGAUUUG U
MassTheoretical: 1.057318625 MDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #3: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGGU GACCAUACGC GAAACUCAGG UGCUGCAAUC U
MassTheoretical: 38.951105 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #4: nucleic-acid, 5.8S ribosomal rRNA

nucleic acidName: 5.8S ribosomal rRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAAU CAUCGAAUCU UUGAACGCAC AUUGCGCCCC UUGGUAUUCC AGGGGGCAUG CCUGUUUGAG CGUCAUU
MassTheoretical: 50.376758 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #5: protein, uL2

ProteinName: uL2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.858875 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #6: protein, uL3

ProteinName: uL3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.491359 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #7: protein, uL4

ProteinName: uL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 38.825762 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #8: protein, uL18

ProteinName: uL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.562559 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #9: protein, eL6

ProteinName: eL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.869369 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #10: protein, uL30

ProteinName: uL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.240377 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #11: protein, eL8

ProteinName: eL8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.814998 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #12: protein, uL6

ProteinName: uL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.691105 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #13: protein, uL16

ProteinName: uL16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.937906 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #14: protein, uL5

ProteinName: uL5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.1369 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #15: protein, eL13

ProteinName: eL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.472967 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #16: protein, eL14

ProteinName: eL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.992792 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #17: protein, eL15

ProteinName: eL15List of Subaru engines / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.221982 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #18: protein, uL13

ProteinName: uL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.028951 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #19: protein, uL22

ProteinName: uL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.245086 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #20: protein, eL18

ProteinName: eL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.421008 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #21: protein, eL19

ProteinName: eL19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.631121 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #22: protein, eL20

ProteinName: eL20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.138432 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #23: protein, eL21

ProteinName: eL21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.034908 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #24: protein, eL22

ProteinName: eL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.263863 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #25: protein, uL14

ProteinName: uL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.005393 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #26: protein, eL24

ProteinName: eL24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.253511 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #27: protein, uL23

ProteinName: uL23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.771176 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #28: protein, uL24

ProteinName: uL24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.06351 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #29: protein, eL27

ProteinName: eL27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.366088 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #30: protein, uL15

ProteinName: uL15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.559393 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #31: protein, eL29

ProteinName: eL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.489611 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #32: protein, eL30

ProteinName: eL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.558344 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #33: protein, eL31

ProteinName: eL31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.292396 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #34: protein, eL32

ProteinName: eL32CD59 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.0225 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #35: protein, eL33

ProteinName: eL33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.932776 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #36: protein, eL34

ProteinName: eL34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.671181 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #37: protein, uL29

ProteinName: uL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.540108 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #38: protein, eL36

ProteinName: eL36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.918959 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #39: protein, eL37

ProteinName: eL37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.629096 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #40: protein, eL38

ProteinName: eL38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.643286 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #41: protein, eL39

ProteinName: eL39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.114286 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #42: protein, eL40

ProteinName: eL40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 5.919163 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #43: protein, eL41

ProteinName: eL41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.137985 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #44: protein, eL42

ProteinName: eL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.655925 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #45: protein, eL43

ProteinName: eL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.611355 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #46: protein, uL1

ProteinName: uL1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.524799 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #47: protein, uL10

ProteinName: uL10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.474707 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #48: protein, L10

ProteinName: L10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.95396 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #49: protein, uS2

ProteinName: uS2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.852168 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #50: protein, eS1

ProteinName: eS1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.410262 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #51: protein, uS5

ProteinName: uS5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.212979 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #52: protein, uS3

ProteinName: uS3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.702791 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #53: protein, eS4

ProteinName: eS4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.039797 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #54: protein, uS7

ProteinName: uS7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.908338 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #55: protein, eS6

ProteinName: eS6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.895072 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #56: protein, eS7

ProteinName: eS7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.000492 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #57: protein, eS8

ProteinName: eS8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.406609 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #58: protein, uS4

ProteinName: uS4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.965434 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #59: protein, eS10

ProteinName: eS10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.585125 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #60: protein, uS17

ProteinName: uS17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.561434 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #61: protein, eS12

ProteinName: eS12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.327331 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #62: protein, uS15

ProteinName: uS15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.928748 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #63: protein, uS11

ProteinName: uS11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.446426 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #64: protein, uS19

ProteinName: uS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.651793 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #65: protein, uS19

ProteinName: uS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.659216 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #66: protein, eS17

ProteinName: eS17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.110388 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #67: protein, uS13

ProteinName: uS13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.928304 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #68: protein, eS19

ProteinName: eS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.81093 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #69: protein, uS10

ProteinName: uS10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.076072 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #70: protein, eS21

ProteinName: eS21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.758829 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #71: protein, uS8

ProteinName: uS8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.518867 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #72: protein, uS12

ProteinName: uS12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.986816 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #73: protein, eS24

ProteinName: eS24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.23165 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #74: protein, eS25

ProteinName: eS25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.001377 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #75: protein, eS26

ProteinName: eS26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.022989 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #76: protein, eS27

ProteinName: eS27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.762195 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #77: protein, eS28

ProteinName: eS28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.116281 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #78: protein, uS14

ProteinName: uS14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.335303 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #79: protein, eS30

ProteinName: eS30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.084264 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #80: protein, eS31

ProteinName: eS31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.457662 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #81: protein, RACK1

ProteinName: RACK1Receptor for activated C kinase 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.710023 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #82: nucleic-acid, 18S ribosomal RNA

nucleic acidName: 18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: AUCUGGUUGA UCCUGCCAGU AGUCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUAUAAGCA AUUUAUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUCGUUUAUU UGAUAGUUCC UUUACUACAU GGUAUAACUG UGGUAAUUCU AGAGCUAAUA ...Sequence:
AUCUGGUUGA UCCUGCCAGU AGUCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUAUAAGCA AUUUAUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUCGUUUAUU UGAUAGUUCC UUUACUACAU GGUAUAACUG UGGUAAUUCU AGAGCUAAUA CAUGCUUAAA AUCUCGACCC UUUGGAAGAG AUGUAUUUAU UAGAUAAAAA AUCAAUGUCU UCGGACUCUU UGAUGAUUCA UAAUAACUUU UCGAAUCGCA UGGCCUUGUG CUGGCGAUGG UUCAUUCAAA UUUCUGCCCU AUCAACUUUC GAUGGUAGGA UAGUGGCCUA CCAUGGUUUC AACGGGUAAC GGGGAAUAAG GGUUCGAUUC CGGAGAGGGA GCCUGAGAAA CGGCUACCAC AUCCAAGGAA GGCAGCAGGC GCGCAAAUUA CCCAAUCCUA AUUCAGGGAG GUAGUGACAA UAAAUAACGA UACAGGGCCC AUUCGGGUCU UGUAAUUGGA AUGAGUACAA UGUAAAUACC UUAACGAGGA ACAAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGCUCCA AUAGCGUAUA UUAAAGUUGU UGCAGUUAAA AAGCUCGUAG UUGAACUUUG GGCCCGGUUG GCCGGUCCGA UUUUUCGUGU ACUGAUUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUGUG GCUCUUGGCG AACCAGGACU UUUACUUUGA AAAAAUUAGA GUGUUCAAAG CAGGCGUAUU GCUCGAAUAU AUUAGCAUGG AAUAAUAGAA UAGGACGUUU GGUUCUAUUU UGUUGGUUUC UAGGACCAUC GUAAUGAUUA AUAGGGACGG UCGGGGGCAU CAGUAUUCAA UUGUCAGAGG UGAAAUUCUU GGAUUUAUUG AAGACUAACU ACUGCGAAAG CAUUUGCCAA GGACGUUUUC AUUAAUCAAG AACGAAAGUU AGGGGAUCGA AGAUGAUCAG AUACCGUCGU AGUCUUAACC AUAAACUAUG CCGACUAGGG AUCGGGUGGU GUUUUUUUAA UGACCCACUC GGCACCUUAC GAGAAAUCAA AGUCUUUGGG UUCUGGGGGG AGUAUGGUCG CAAGGCUGAA ACUUAAAGGA AUUGACGGAA GGGCACCACC AGGAGUGGAG CCUGCGGCUU AAUUUGACUC AACACGGGGA AACUCACCAG GUCCAGACAC AAUAAGGAUU GACAGAUUGA GAGCUCUUUC UUGAUUUUGU GGGUGGUGGU GCAUGGCCGU UCUUAGUUGG UGGAGUGAUU UGUCUGCUUA AUUGCGAUAA CGAACGAGAC CUUAACCUAC UAAAUAGUGG UGCUAGCAUU UGCUGGUUAU CCACUUCUUA GAGGGACUAU CGGUUUCAAG CCGAUGGAAG UUUGAGGCAA UAACAGGUCU GUGAUGCCCU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUUGU GAAACUCCGU CGUGCUGGGG AUAGAGCAUU GUAAUUAUUG CUCUUCAACG AGGAAUUCCU AGUAAGCGCA AGUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGAU CUGCUUAGAG AAGGGGGCAA CUCCAUCUCA GAGCGGAGAA UUUGGACAAA CUUGGUCAUU UAGAGGAACU AAAAGUCGUA ACAAGGUUUC CGUAGGUGAA CCUGCGGAAG GAUCAU
MassTheoretical: 578.475125 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #83: protein, eIF5B

ProteinName: eIF5B / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 67.068273 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Escherichia coli (E. coli)

+
Component #84: nucleic-acid, Met-tRNA-iMet

nucleic acidName: Met-tRNA-iMet / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AGCGCCGUGG CGCAGUGGGA AGCGCGCAGG GCUCAUAACC CUGAUGUCCU CGGAUCGAAA CCGAGCGGCG CUACCA
MassTheoretical: 24.583705 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #85: nucleic-acid, mRNA

nucleic acidName: mRNAMessenger RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAAAUG
MassTheoretical: 1.923237 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

+
Component #86: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 6 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

+
Component #87: ligand, GUANOSINE-5'-DIPHOSPHATE

LigandName: GUANOSINE-5'-DIPHOSPHATE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.443201 kDa

+
Component #88: ligand, N-carboxy-L-threonine

LigandName: N-carboxy-L-threonine / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.163129 kDa

+
Component #89: ligand, METHIONINE

LigandName: METHIONINE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.149211 kDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7
Support filmunspecified
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 60 e/Å2 / Illumination mode: OTHER
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: OTHER

-
Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 107000
3D reconstructionSoftware: RELION / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Refinement protocol: rigid body
Input PDB model: 4V8Y
Output model

+
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