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データを開く
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基本情報
登録情報 | ![]() | ||||||||||||
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タイトル | Active state CI from Rotenone-NADH dataset, Subclass 1 | ||||||||||||
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機能・相同性 | ![]() Mitochondrial protein import / RHOG GTPase cycle / Complex I biogenesis / Respiratory electron transport / Neutrophil degranulation / Mitochondrial protein degradation / cardiac muscle tissue development / ubiquinone-6 biosynthetic process / respiratory chain complex I / oxidoreductase activity, acting on NAD(P)H ...Mitochondrial protein import / RHOG GTPase cycle / Complex I biogenesis / Respiratory electron transport / Neutrophil degranulation / Mitochondrial protein degradation / cardiac muscle tissue development / ubiquinone-6 biosynthetic process / respiratory chain complex I / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / acyl binding / ubiquinone binding / acyl carrier activity / quinone binding / electron transport coupled proton transport / : / ATP synthesis coupled electron transport / negative regulation of intrinsic apoptotic signaling pathway / mitochondrial ATP synthesis coupled electron transport / mitochondrial respiratory chain complex I assembly / respiratory electron transport chain / reactive oxygen species metabolic process / : / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase (ubiquinone) activity / electron transport chain / aerobic respiration / mitochondrial intermembrane space / negative regulation of cell growth / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / nervous system development / 4 iron, 4 sulfur cluster binding / response to oxidative stress / nuclear body / mitochondrial inner membrane / nuclear speck / mitochondrial matrix / protein-containing complex binding / endoplasmic reticulum / mitochondrion / nucleoplasm / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() ![]() ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.0 Å | ||||||||||||
![]() | Gu JK / Yang MJ | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: The coupling mechanism of mammalian mitochondrial complex I. 著者: Jinke Gu / Tianya Liu / Runyu Guo / Laixing Zhang / Maojun Yang / ![]() 要旨: Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in ...Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the present study, we report cryo-electron microscopy structures of CI from Sus scrofa in six treatment conditions at a resolution of 2.4-3.5 Å, in which CI structures of each condition can be classified into two biochemical classes (active or deactive), with a notably higher proportion of active CI particles. These structures illuminate how hydrophobic ubiquinone-10 (Q10) with its long isoprenoid tail is bound and reduced in a narrow Q chamber comprising four different Q10-binding sites. Structural comparisons of active CI structures from our decylubiquinone-NADH and rotenone-NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. Our data overturn the widely accepted previous proposal about the coupling mechanism of CI. | ||||||||||||
履歴 |
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構造の表示
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-EMDBアーカイブ
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-検証レポート
文書・要旨 | ![]() | 384.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 383.7 KB | 表示 | |
XML形式データ | ![]() | 6.5 KB | 表示 | |
CIF形式データ | ![]() | 7.4 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 7w1vMC ![]() 7v2cC ![]() 7v2dC ![]() 7v2eC ![]() 7v2fC ![]() 7v2hC ![]() 7v2kC ![]() 7v2rC ![]() 7v30C ![]() 7v31C ![]() 7v32C ![]() 7v33C ![]() 7v3mC ![]() 7vb7C ![]() 7vblC ![]() 7vbnC ![]() 7vbpC ![]() 7vbzC ![]() 7vc0C ![]() 7vwjC ![]() 7vwlC ![]() 7vxpC ![]() 7vxsC ![]() 7vxuC ![]() 7vy1C ![]() 7vy8C ![]() 7vy9C ![]() 7vyaC ![]() 7vyeC ![]() 7vyfC ![]() 7vygC ![]() 7vyhC ![]() 7vyiC ![]() 7vynC ![]() 7vysC ![]() 7vz1C ![]() 7vz8C ![]() 7vzvC ![]() 7vzwC ![]() 7w00C ![]() 7w0hC ![]() 7w0rC ![]() 7w0yC ![]() 7w1oC ![]() 7w1pC ![]() 7w1tC ![]() 7w1uC ![]() 7w1zC ![]() 7w20C ![]() 7w2kC ![]() 7w2lC ![]() 7w2rC ![]() 7w2uC ![]() 7w2yC ![]() 7w31C ![]() 7w32C ![]() 7w35C ![]() 7w4cC ![]() 7w4dC ![]() 7w4eC ![]() 7w4fC ![]() 7w4gC ![]() 7w4jC ![]() 7w4kC ![]() 7w4lC ![]() 7w4mC ![]() 7w4nC ![]() 7w4qC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.0742 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : The porcine respiratory complex I
+超分子 #1: The porcine respiratory complex I
+分子 #1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
+分子 #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
+分子 #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
+分子 #4: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
+分子 #5: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
+分子 #6: Acyl carrier protein
+分子 #7: Complex I subunit B13
+分子 #8: Complex I-B14.5a
+分子 #9: NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 9, mitoc...
+分子 #10: Complex I-9kD
+分子 #11: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
+分子 #12: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
+分子 #13: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
+分子 #14: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
+分子 #15: Complex I-30kD
+分子 #16: Complex I-49kD
+分子 #17: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
+分子 #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
+分子 #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
+分子 #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
+分子 #21: Complex I-B16.6
+分子 #22: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...
+分子 #23: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
+分子 #24: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...
+分子 #25: Complex I-B17
+分子 #26: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...
+分子 #27: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
+分子 #28: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...
+分子 #29: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
+分子 #30: NADH dehydrogenase [ubiquinone] 1 subunit C2
+分子 #31: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
+分子 #32: NADH-ubiquinone oxidoreductase chain 2
+分子 #33: NADH-ubiquinone oxidoreductase chain 3
+分子 #34: NADH-ubiquinone oxidoreductase chain 4L
+分子 #35: NADH-ubiquinone oxidoreductase chain 5
+分子 #36: NADH-ubiquinone oxidoreductase chain 6
+分子 #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
+分子 #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
+分子 #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
+分子 #40: NADH-ubiquinone oxidoreductase chain 4
+分子 #41: NADH-ubiquinone oxidoreductase chain 1
+分子 #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
+分子 #43: Complex I-B18
+分子 #44: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...
+分子 #45: IRON/SULFUR CLUSTER
+分子 #46: FLAVIN MONONUCLEOTIDE
+分子 #47: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE
+分子 #48: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
+分子 #49: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,...
+分子 #50: (2R,6aS,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahy...
+分子 #51: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...
+分子 #52: CARDIOLIPIN
+分子 #53: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
+分子 #54: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer
+分子 #55: FE2/S2 (INORGANIC) CLUSTER
+分子 #56: MAGNESIUM ION
+分子 #57: ZINC ION
+分子 #58: ADENOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm / 最小 デフォーカス(公称値): 1.3 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.0 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 63357 |
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初期 角度割当 | タイプ: RANDOM ASSIGNMENT |
最終 角度割当 | タイプ: RANDOM ASSIGNMENT |