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- EMDB-32309: Deactive state CI from Q1-NADH dataset, Subclass 5 -

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Basic information

Entry
Database: EMDB / ID: EMD-32309
TitleDeactive state CI from Q1-NADH dataset, Subclass 5
Map data
Sample
  • Complex: Respiratory complex I
    • Protein or peptide: x 44 types
  • Ligand: x 13 types
Keywordsmammalian / mitochondrial / respiratory / complex I / ELECTRON TRANSPORT
Function / homology
Function and homology information


RHOG GTPase cycle / Mitochondrial protein import / Complex I biogenesis / Respiratory electron transport / Neutrophil degranulation / cardiac muscle tissue development / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly ...RHOG GTPase cycle / Mitochondrial protein import / Complex I biogenesis / Respiratory electron transport / Neutrophil degranulation / cardiac muscle tissue development / oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / negative regulation of intrinsic apoptotic signaling pathway / respirasome / aerobic respiration / reactive oxygen species metabolic process / respiratory electron transport chain / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / negative regulation of cell growth / fatty acid biosynthetic process / NAD binding / FMN binding / nervous system development / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / nuclear body / mitochondrial matrix / nuclear speck / endoplasmic reticulum / mitochondrion / nucleoplasm / metal ion binding / cytosol / cytoplasm
Similarity search - Function
NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / Tim17/Tim22/Tim23/Pmp24 family / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 ...NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / Tim17/Tim22/Tim23/Pmp24 family / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / GRIM-19 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NDUFA6, LYR domain / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Deoxynucleoside kinase domain / Deoxynucleoside kinase / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH dehydrogenase, subunit C / NADH ubiquinone oxidoreductase, F subunit / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / 2Fe-2S iron-sulfur cluster binding domain / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region
Similarity search - Domain/homology
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 ...NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 4L / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4
Similarity search - Component
Biological speciesSus scrofa (pig) / pig (pig) / Pig (pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsGu J / Yang M
Funding support China, 3 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2017YFA0504600 and 2016YFA0501100 China
National Natural Science Foundation of China (NSFC)31625008 China
National Natural Science Foundation of China (NSFC)32030056, 21532004 and 31570733 and 31800620 China
CitationJournal: Nat Struct Mol Biol / Year: 2022
Title: The coupling mechanism of mammalian mitochondrial complex I.
Authors: Jinke Gu / Tianya Liu / Runyu Guo / Laixing Zhang / Maojun Yang /
Abstract: Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in ...Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the present study, we report cryo-electron microscopy structures of CI from Sus scrofa in six treatment conditions at a resolution of 2.4-3.5 Å, in which CI structures of each condition can be classified into two biochemical classes (active or deactive), with a notably higher proportion of active CI particles. These structures illuminate how hydrophobic ubiquinone-10 (Q10) with its long isoprenoid tail is bound and reduced in a narrow Q chamber comprising four different Q10-binding sites. Structural comparisons of active CI structures from our decylubiquinone-NADH and rotenone-NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. Our data overturn the widely accepted previous proposal about the coupling mechanism of CI.
History
DepositionNov 28, 2021-
Header (metadata) releaseJan 25, 2023-
Map releaseJan 25, 2023-
UpdateJun 28, 2023-
Current statusJun 28, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32309.png

Downloads & links

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Map

FileDownload / File: emd_32309.map.gz / Format: CCP4 / Size: 107.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.0979 Å
Density
Contour LevelBy AUTHOR: 0.03
Minimum - Maximum-0.12025829 - 0.20456213
Average (Standard dev.)0.00054426055 (±0.0066438243)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions304304304
Spacing304304304
CellA=B=C: 333.7616 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Respiratory complex I

EntireName: Respiratory complex I
Components
  • Complex: Respiratory complex I
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: Complex I subunit B13Respiratory complex I
    • Protein or peptide: Complex I-B14.5a
    • Protein or peptide: NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 9, mitochondrial
    • Protein or peptide: Complex I-9kD
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
    • Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
    • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
    • Protein or peptide: Complex I-30kD
    • Protein or peptide: Complex I-49kD
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
    • Protein or peptide: Complex I-B16.6
    • Protein or peptide: Complex I-AGGG
    • Protein or peptide: Complex I-B12
    • Protein or peptide: Complex I-SGDH
    • Protein or peptide: Complex I-B17
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
    • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
    • Protein or peptide: Complex I-B18
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE
  • Ligand: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
  • Ligand: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL
  • Ligand: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate
  • Ligand: CARDIOLIPIN
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer
  • Ligand: ADENOSINE-5'-DIPHOSPHATE

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Supramolecule #1: Respiratory complex I

SupramoleculeName: Respiratory complex I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Sus scrofa (pig)

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Macromolecule #1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 47.434066 KDa
SequenceString: APKKTSFGSL KDEDRIFTNL YGRHDWRLKG AQSRGDWYKT KEILLKGPDW ILGEVKTSGL RGRGGAGFPT GLKWSFMNKP SDGRPKYLV VNADEGEPGT CKDREIIRHD PHKLVEGCLV GGRAMGARAA YIYIRGEFYN EASNLQVAIR EAYEAGLIGK N ACGSGYDF ...String:
APKKTSFGSL KDEDRIFTNL YGRHDWRLKG AQSRGDWYKT KEILLKGPDW ILGEVKTSGL RGRGGAGFPT GLKWSFMNKP SDGRPKYLV VNADEGEPGT CKDREIIRHD PHKLVEGCLV GGRAMGARAA YIYIRGEFYN EASNLQVAIR EAYEAGLIGK N ACGSGYDF DVFVVRGAGA YICGEETALI ESIEGKQGKP RLKPPFPADV GVFGCPTTVA NVETVAVSPT ICRRGGAWFA SF GRERNSG TKLFNISGHV NHPCTVEEEM SVPLKELIEK HAGGVIGGWD NLLAVIPGGS STPLIPKSVC ETVLMDFDAL VQA QTGLGT AAVIVMDRST DIVKAIARLI EFYKHESCGQ CTPCREGVDW MNKVMARFVK GDARPAEIDS LWEISKQIEG HTIC ALGDG AAWPVQGLIR HFRPELEERM QQFALQHQ

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Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 20.207957 KDa
SequenceString:
TYKFVNMREP SMDMKSVTDR AAQTLLWTEL VRGLGMTLSY LFREPATINY PFEKGPLSPR FRGEHALRRY PSGEERCIAC KLCEAVCPA QAITIEAEPR ADGSRRTTRY DIDMTKCIYC GFCQEACPVD AIVEGPNFEF STETHEELLY NKEKLLNNGD K WEAEIAAN IQADYLYR

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Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 17.874953 KDa
SequenceString:
SRGEYVVAKL DDLVNWARRS SLWPMTFGLA CCAVEMMHMA APRYDMDRFG VVFRASPRQS DVMIVAGTLT NKMAPALRKV YDQMPEPRY VVSMGSCANG GGYYHYSYSV VRGCDRIVPV DIYVPGCPPT AEALLYGILQ LQRKIKREKR LRIWYRR

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Macromolecule #4: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 13.812977 KDa
SequenceString:
GTSVKPIFSR DMNEAKRRVR ELYRAWYREV PNTVHLFQLD ISVKQGRDKV REMFMKNAHV TDPRVVDLLV IKGKMELEET INVWKQRTH IMRFFHETEA PRPTDFLSKF YVGHDP

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Macromolecule #5: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 9.84128 KDa
SequenceString:
LGLREIRIHL CQRSPGSQGV RDFIEKRYVE LKKANPDLPI LIRECSDVQP KLWARYAFGQ EKNVSLNNFS ADQVTRTLEN VLSGKA

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Macromolecule #6: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 10.133566 KDa
SequenceString:
SDAPPLTLEA IKDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

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Macromolecule #7: Complex I subunit B13

MacromoleculeName: Complex I subunit B13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 12.949106 KDa
SequenceString:
LKKTTGLVGL AVCETPHERL KILYTKILDV LGQIPKNAAY RKYTEQITNE KLGMVKAEPD VKKLEEQLQG GQIEEVILQA ENELSLARK MLRWKPWEPL VEEPPANQWK WPI

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Macromolecule #8: Complex I-B14.5a

MacromoleculeName: Complex I-B14.5a / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 12.517394 KDa
SequenceString:
ASATRVIQLL RNWASGRDLQ AKLQLRYQEI SKRTQPPPKL PVGPSHKLSN NYYCTRDGRR EAMPPSIVMS SQKVLASGKP AESSAVAET EKKAVTPAPP IKRWELSKDQ PYL

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Macromolecule #9: NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 9, mitoc...

MacromoleculeName: NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 9, mitochondrial
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 38.954051 KDa
SequenceString: LHHALIPHGK GGRSSVSGIV ATVFGATGFL GRYVVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MEWNGKDKDS IRKVVEHSN VVINLVGREW ETKNFDFEDV FVKIPHAIAQ VSKEAGVEKL IHISHLNADI KSPSRYLRSK AVGEKEVRAA F PEATIIKP ...String:
LHHALIPHGK GGRSSVSGIV ATVFGATGFL GRYVVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MEWNGKDKDS IRKVVEHSN VVINLVGREW ETKNFDFEDV FVKIPHAIAQ VSKEAGVEKL IHISHLNADI KSPSRYLRSK AVGEKEVRAA F PEATIIKP SDIFGREDRF LNYFASMRWF GGVPLISLGK ETVKQPVYIV DVSKGIINAI KDPDAKGKTF AFVGPNRYLL FD LVQYIFA VAYRPFLPYP LPHFAYRWVG RLFEVSPFEP WTTRDKVERV HMSDMTLPHL PGLEDLGIQA TPLELKAIEV LRR HRTYRW LTSEMEDVKP AKTVNI

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Macromolecule #10: Complex I-9kD

MacromoleculeName: Complex I-9kD / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 5.193683 KDa
SequenceString:
FDNSTYRNLQ HHEYSTYTFL DLNVELSKFR MPQPSSGRQS PRH

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Macromolecule #11: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 14.442389 KDa
SequenceString:
QLIAVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQSG VNNTKKWKME FDTRERWENP LMGWSSTADP LSNLVLTFST KEDAVAFAE KNGWSFDVEE RKVPKPKSKS YGANFSWNKR TRVSTK

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Macromolecule #12: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

MacromoleculeName: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 75.770773 KDa
SequenceString: SNLIEVFVDG QSVMVEPGTT VLQACEKVGM QIPRFCYHER LSVAGNCRMC LVEIEKAPKV VAACAMPVMK GWNILTNSEK SKKAREGVM EFLLANHPLD CPICDQGGEC DLQDQSMMFG SDRSRFLEGK RAVEDKNIGP LVKTIMTRCI QCTRCIRFAS E IAGVDDLG ...String:
SNLIEVFVDG QSVMVEPGTT VLQACEKVGM QIPRFCYHER LSVAGNCRMC LVEIEKAPKV VAACAMPVMK GWNILTNSEK SKKAREGVM EFLLANHPLD CPICDQGGEC DLQDQSMMFG SDRSRFLEGK RAVEDKNIGP LVKTIMTRCI QCTRCIRFAS E IAGVDDLG TTGRGNDMQV GTYIEKMFMS ELSGNIIDIC PVGALTSKPY AFTARPWETR KTESIDVMDA VGSNIVVSTR TG EVMRILP RMHEDINEEW ISDKTRFAYD GLKRQRLTQP MIRNEKGLLT YTTWEDALSR VAGMLQSFQG NDVAAIAGGL VDA EALVAL KDLLNRVDSD SLCTEEVFPT AGAGTDLRSN YLLNTTIAGV EEADVILLVG TNPRFEAPLF NARIRKSWLH NDLK VALIG SPVDLTYRYD HLGDSPKILQ DIASGNHPFS QILKEAKKPM VVLGSSALQR SDGTAILAAV SNIAQNIRLS SGVTG DWKV MNILHRIASQ VAALDLGYKP GVEAIRKNPP KVLFLLGADG GCITRQDLPK DCFIIYQGHH GDVGAPMADV ILPGAA YTE KSATYVNTEG RAQQTKVAVT PPGLAREDWK IIRALSEIAG MTLPYDTLDQ VRSRLEEVSP NLVRYDDVEG ANYFQQA NE LSKLVNQQLL ADPLVPPQLT IKDFYMTDSI SRASQTMAKC VKAVTEGI

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Macromolecule #13: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 17.031244 KDa
SequenceString:
ELVQVLRRGL QQVSGHGGLR GYLRVLFRAN DVRVGTLVGE DKYGNKYYED NKQFFGRHRW VIYTTEMNGR DTFWDVDGSM VPPEWHRWL HCMTDDPPTT KPPTARKYIW TNHKFNVSGT PQQYVPYSTT RKKIQEWVPP STPYK

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Macromolecule #14: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 23.826336 KDa
SequenceString: GAGGALFVHR DTPENNPDTP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD ...String:
GAGGALFVHR DTPENNPDTP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL

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Macromolecule #15: Complex I-30kD

MacromoleculeName: Complex I-30kD / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 24.52173 KDa
SequenceString: TRPTIRPRND VVHKQLSAFG QYVAEILPKY VQQVQVSCFN ELEIFIHPDG VIPVLTFLRD HTNAQFKSLA DLTAVDVPTR QNRFEIVYN LLSLRFNSRI RVKTYTDELT PIESSVTVYK AANWYEREIW DMFGVFFANH PDLRRILTDY GFEGHPFRKD F PLSGYVEL ...String:
TRPTIRPRND VVHKQLSAFG QYVAEILPKY VQQVQVSCFN ELEIFIHPDG VIPVLTFLRD HTNAQFKSLA DLTAVDVPTR QNRFEIVYN LLSLRFNSRI RVKTYTDELT PIESSVTVYK AANWYEREIW DMFGVFFANH PDLRRILTDY GFEGHPFRKD F PLSGYVEL RYDDEVKRVV AEPVELAQEF RKFDLNSPWE AFPAYRQPPE

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Macromolecule #16: Complex I-49kD

MacromoleculeName: Complex I-49kD / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 49.234324 KDa
SequenceString: ARQWQPDVEW AEQFGGAVMY PTKETAHWKP PPWNDVDPPK DTLVSNLTLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLH(2MR)GTEK LIEYKTYLQA LPYFDRLDYV SMMCNEQAYS LAVEKLLNIQ PPPRAQWIRV LFGEITRLLN HIMAVTT HA LDIGAMTPFF ...String:
ARQWQPDVEW AEQFGGAVMY PTKETAHWKP PPWNDVDPPK DTLVSNLTLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLH(2MR)GTEK LIEYKTYLQA LPYFDRLDYV SMMCNEQAYS LAVEKLLNIQ PPPRAQWIRV LFGEITRLLN HIMAVTT HA LDIGAMTPFF WMFEEREKMF EFYERVSGAR MHAAYIRPGG VHQDLPLGLL DDIYEFSKNF SFRIDELEEM LTNNRIWR N RTVDIGVVTA EDALNYGFSG VMLRGSGIQW DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIISQCLN KMPPGEIKVD DAKVSPPKRA EMKTSMESLI HHFKLYTEGY QVPPGATYTA IEAPKGEFGV YLVSDGSSRP YRCKIKAPGF AHLAGLDKM SKGHMLADVV AIIGTQDIVF GEVDR

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Macromolecule #17: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 8.088424 KDa
SequenceString:
MWFEILPGIA VMAACLFIPG MATAHIHKFT NGGKEKRVAH FSYQWNLMER DRRISGVNRY HVTKGLENID

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Macromolecule #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 10.567635 KDa
SequenceString:
GVRTSPTGEK VTHTGQAYDD GDYRRVRFSD RQKEVNENFA IDLIAEQPVS EVGSRVISCD GGGGALGHPR VYINLDKETK TGTCGYCGL QFRQPHH

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Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 9.094372 KDa
SequenceString:
AGRIASFLKN AWAKEPVLVA SFAIGGLAII LPSLSPYTNY AIRINRATPY NYPVPLRDDG NMPDVPSHPQ DPQGPSLEWL KNL

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Macromolecule #20: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 14.555698 KDa
SequenceString:
AKTLLHKYSD IPEGTECHRK AYASTSIGGA TGLIVSAYSI ALKPPASFLE GVARTGRYTF TSAAIGAIFG LTSCISAQVR EKPDDPLNY FIGGCAGGLT LGARTRSYGI GAAACAYMGL TAALVKMGQL EGWQVFAEPK V

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Macromolecule #21: Complex I-B16.6

MacromoleculeName: Complex I-B16.6 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 16.70934 KDa
SequenceString:
ASKVKQDMPP PGGYGPIDYK RNLPRRGLSG YSMFAVGIGT LLFGYWSMMK WNRERRRLQI EDFEARIALM PLFQAEKDRR VLQMLRENL EEEAIIMKDV PDWKVGESVF HTTRWVTPMM GELYGLRTNE EILSATYGFI WYT

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Macromolecule #22: Complex I-AGGG

MacromoleculeName: Complex I-AGGG / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 8.430282 KDa
SequenceString:
GGVHIEPRYR QFPQLTRSQL IQAEFFSATM WFWILWRFWH DSDAVLGHFP YPDPSQWTDE ELGILPDDED

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Macromolecule #23: Complex I-B12

MacromoleculeName: Complex I-B12 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 9.504672 KDa
SequenceString:
EHGHSKMELP DYKQWKIEGT PLETVQEKLA ARGLRDPWGR NEAWRYSGGF ANNVSFVGAL LKGFKWGFAA FVVAVGAEYY LESQ

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Macromolecule #24: Complex I-SGDH

MacromoleculeName: Complex I-SGDH / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 16.477098 KDa
SequenceString:
HGKRLFIIKP SGFYDRRFLK LMRFYILLTG IPVAIGITLV NVFIGEAELA DIPEGYVPEH WEYFKHPISR WIARTFYDGP EKNYEKTMA ILQIEAEKAE LRLKELEVRR LMRARGDGPW YQYPTIDKAL IDHSPKTTPD N

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Macromolecule #25: Complex I-B17

MacromoleculeName: Complex I-B17 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 15.333832 KDa
SequenceString:
SGYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRVW PMEQFWNKFL QDGAPWKNVI YKTYRHSIFA VTHVLIPVWI IHYYLKYHV TAKPYTVVER KPRIFPGDTI LETGEVIPLM KEFPDQH

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Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 18.608805 KDa
SequenceString:
HVTKDMFPGP YPKTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPKLPD RSQQERDPWY DWDHPDLRLN WGEPIHWDLD MYIRNRVDT SPTPVSWNTM CKHLFGFVAF MLFMFWVGEI YPSYQPVGPK QYPYNDLYLE RGGDPTKEPE PVVHYEI

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Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 20.813572 KDa
SequenceString:
PDSWDKDVYP EPPRRTPAPA PQTSLPNPVT YLTKIFDLLV DRPVTLAREF IEQQHAKNRY YYYHREFRRV PDITECEEKD ILCMFEAEM QWRRDYKVDQ EIVNIIQERL KACQQREGES YRQNCAKELE QFTQVSKAFQ DRYSDLGAHY SARKCLAKQK Q RMLAERKA AKEAAAA

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Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 12.635055 KDa
SequenceString:
PPEPTIRLQE DPDPEDENLY EKNPDSHGYD KDPIVDLWNM RVVFFFGFSI VLVLGSTFVA YLPDYRMQEW ARREAERLVK YREANGLPL MESNCFDPNK IQLPEDED

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Macromolecule #29: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 5.881825 KDa
SequenceString:
KFYIREPPHG SPDWLKVGLT LGTSVFLWIY LIKQHKEDVL EYKRRNGLE

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Macromolecule #30: NADH dehydrogenase [ubiquinone] 1 subunit C2

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 14.327655 KDa
SequenceString:
MTMMSGRPGR VPLQFLPNEA RSLPPPKLTD PRLVYMGFLG YCSGLIDNAI RRRPVVSAGL HRQLLYVTSF VFFGYYLLKR QDYMYALRD HDMFAYVKSH PEDFPEKDKK TYGEILEEFH PVR

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Macromolecule #31: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

MacromoleculeName: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 12.375395 KDa
SequenceString:
PFFDVQKRLG LDLDRWMTIQ SAEQPHKIPG RCHAFEKEWI ECAHGIGGIR AEKECKIEFD DFVECLLRQK TMKRLSAIKR QRDKLIKEG KYTPPPHHLG KEDPRP

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Macromolecule #32: NADH-ubiquinone oxidoreductase chain 2

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 39.077504 KDa
SequenceString: MNPIIYTTLI MTVMSGTMLV MISSHWLLIW IGFEMNLLAM IPVLMKNFNP RATEAATKYF LTQATASMML MMAIIINLLY SGQWTITKM FNPVAMTMMT MALAMKLGLS PFHFWVPEVT QGISLQAGLL LLTWQKLAPL SVLCQISQSI NPNLMLTMAM L SILIGGWG ...String:
MNPIIYTTLI MTVMSGTMLV MISSHWLLIW IGFEMNLLAM IPVLMKNFNP RATEAATKYF LTQATASMML MMAIIINLLY SGQWTITKM FNPVAMTMMT MALAMKLGLS PFHFWVPEVT QGISLQAGLL LLTWQKLAPL SVLCQISQSI NPNLMLTMAM L SILIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLPYNT TMTILNLLIY ITMTLAMFML LIHSSATTTL SLSHTWNKMP VI TSLMMVT LLSMGGLPPL SGFMPKWMII QEMTKNESII MPTLMAMTAL LNLYFYMRLA YSSSLTMFPS TNNMKMKWQF EHT KQMKLL PTMIVLSTLV LPMTPALSSL N

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Macromolecule #33: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 12.998448 KDa
SequenceString:
MNIMLTLLTN VTLASLLVLI AFWLPQLNAY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TNNLKTMLT MALFLLILLA ASLAYEWTQK GLEWAE

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Macromolecule #34: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 10.827253 KDa
SequenceString:
MPLVYMNIIM AFAIALAGLL MYRSHLMSSL LCLEGMMLSL FIMSTLIILN THFTLANMMP IILLVFAACE AALGLSLLVM VSNTYGTDY VQNLNLLQC

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Macromolecule #35: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 68.267031 KDa
SequenceString: MNPFASLTLT TLTILTIPIM MSNSNIYKTN LYPNYVKTTV SYAFTLSLVP LLMFMHTGQE MIISNWHWMT LQTVELSLSF KMDYFSVMF IPVALFVTWS IMEFSMWYMH SDPFINRFFK YLLLFLITMM ILVTANNLFQ LFIGWEGVGI MSFLLIGWWH G RTDANTAA ...String:
MNPFASLTLT TLTILTIPIM MSNSNIYKTN LYPNYVKTTV SYAFTLSLVP LLMFMHTGQE MIISNWHWMT LQTVELSLSF KMDYFSVMF IPVALFVTWS IMEFSMWYMH SDPFINRFFK YLLLFLITMM ILVTANNLFQ LFIGWEGVGI MSFLLIGWWH G RTDANTAA LQAILYNRIG DIGFVLSMAW FLTHSNAWDL QQIFMLNNEC PNMPLIGLLL AAAGKSAQFG LHPWLPSAME GP TPVSALL HSSTMVVAGV FLLIRFYPLM ETNKLVQTMT LCLGAITTLF TALCAITQND IKKIVAFSTS SQLGLMMVTI GIN QPHLAF LHICMHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLYKAM PFTTTALIIG SLALTGMPYL TGFYSKDLII EAVN MSYTN AWALLMTLIA TSLTAAYSTR IIFFAFLGKP RFPPLVLINE NNPLLINSIK RLLIGSIFAG FIISNNIPPM TVPNT TMPL YMKMTALIVT IMGFMLALEL NNTTYYLKFK YPSQTYKFSN MLGYYPSIMH RLPTYHNLSM SQKSASSLLD LIWLET ILP KTTSFIQMKM SIMVSNQKGL IKLYFLSFLI TIMISMTLFN

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Macromolecule #36: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 19.021332 KDa
SequenceString:
MTMYIAFILS TIFVIGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMLVVFGY TTAMATEMYP EVWVSNKTV FGAFVSGLMM EFCMVYYALK EEEVEIIFKF NGLGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

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Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 6.781886 KDa
SequenceString:
NVLQIVRDHW VHILVPVGFV FGCYLDRRSD EKLTAFRNKS LLFKRELRPN EEVTWK

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Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 14.986153 KDa
SequenceString:
SFPKYKPSRL ATLPATLDPA EYDISPETRK AQAERLAIRS RLKREYLLQY NDPNRLGVIE DPALIRWTYA RSANIYPNFR PTPKTSLLG ALFGIGPLFF WYYVFKTDRD KKEKLIQEGK LDQTFNISY

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Macromolecule #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 21.733711 KDa
SequenceString:
AFSAPAAYLT HQQKVLRLYK RALRHLESWC VHRDKYRYFA CLMRARFDEH KNEKDMVKAT QLLRQAEEEF WYGQHPQPYI FPESPGGTS YERYECYKVP EWCLDDWHPS EKAMYPDYFA KREQWKRLRR ESWEREVKQL QEETPPGGPR TEALPPARKE G DLPPLWWH IVTRPRERPM

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Macromolecule #40: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 51.853355 KDa
SequenceString: MLKIIIPTTM LLPMTWMSKH NMIWINATVH SLLISLISLS LLNQLGENSL NFSLTFFSDS LSAPLLVLTT WLLPLMLMAS QSHLSKETT TRKKLYITML ILLQLFLIMT FTATELILFY ILFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALVYIQ ...String:
MLKIIIPTTM LLPMTWMSKH NMIWINATVH SLLISLISLS LLNQLGENSL NFSLTFFSDS LSAPLLVLTT WLLPLMLMAS QSHLSKETT TRKKLYITML ILLQLFLIMT FTATELILFY ILFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALVYIQ NTTGSLNFLI IHYWSHPLSN SWSNIFMWLA CIMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAVLLKL GG YGMMRIT TILNPLTNYM AYPFLMLSMW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAIMIQTPW SFMGATALMI AHG LTSSML FCLANTNYER VHSRTMILAR GLQTLLPLMA TWWLVASLTN LALPPSINLI GELFIITASF SWSNITIILM GMNM MITAL YSLYMLITTQ RGKYTHHINN IKPSFTRENA LMALHILPLL LLTLNPKMIL GPLY

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Macromolecule #41: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 35.66752 KDa
SequenceString: MFMINILSLI IPILLAVAFL TLVERKVLGY MQLRKGPNVV GPYGLLQPIA DALKLFTKEP LRPATSSISM FIIAPILALS LALTMWVPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS Y TLSTLITT ...String:
MFMINILSLI IPILLAVAFL TLVERKVLGY MQLRKGPNVV GPYGLLQPIA DALKLFTKEP LRPATSSISM FIIAPILALS LALTMWVPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS Y TLSTLITT QEHIWMIFTS WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFAMFFMAEY ANIIMMNAFT AI LFLGASH DPHTPELYTI NFVLKTLALT ITFLWIRASY PRFRYDQLMH LLWKSFLPLT LALCMWHISL PIMTASIPPQ S

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Macromolecule #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 19.932898 KDa
SequenceString:
PGIVELPTLE DLKVQEVKVS SSVLKAAAHH YGAQCDKPNK EFMLCRWEEK DPRRCLEEGK LVNQCALDFF RQIKRHCAEP FTEYWTCID YSGLQLFRHC RKQQAKFDEC VLDKLGWVRP DLGELSKVTK VKTDRPLPEN PYHSRARPEP NPEAEGDLKP A KHGSRLFF WTM

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Macromolecule #43: Complex I-B18

MacromoleculeName: Complex I-B18 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 15.176357 KDa
SequenceString:
GAHLARRYLG DASKEPDPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL MLQQRDYCAH YLIQLLKCKR DSFPNFLACK HEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA EMARG

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Macromolecule #44: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: pig (pig)
Molecular weightTheoretical: 36.79268 KDa
SequenceString: LQYGPLAFVL GERTTRKLTE TSKVITVDGN ICSGKGRLAR EIAEKLGLRH FPEAGIHYAD STTGDGKPLD VQLSGNCSLE KFYDDPKSN DGNSYRLQSW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV EHYNEVKKVT A CEYLPPHV ...String:
LQYGPLAFVL GERTTRKLTE TSKVITVDGN ICSGKGRLAR EIAEKLGLRH FPEAGIHYAD STTGDGKPLD VQLSGNCSLE KFYDDPKSN DGNSYRLQSW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV EHYNEVKKVT A CEYLPPHV VVYVDVPVPE IQSRIQKKGN PHEMKITAAY LQDIENAYKK TFLPEMSEKC EVLQYSAREA EDAEKVVEDI EY LKCDKGP WPDQDDRTFH RLRMLVQNKL EVLNYTTIPV YLPEITIGAH QSDRVFQKFT ELPGRKYSPG YNEDVGDKWI WLK

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Macromolecule #45: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 45 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #46: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 46 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

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Macromolecule #47: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

MacromoleculeName: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / type: ligand / ID: 47 / Number of copies: 1 / Formula: NAI
Molecular weightTheoretical: 665.441 Da
Chemical component information

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / Nicotinamide adenine dinucleotide

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Macromolecule #48: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

MacromoleculeName: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / type: ligand / ID: 48 / Number of copies: 7 / Formula: PEE
Molecular weightTheoretical: 744.034 Da
Chemical component information

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM / Discrete optimized protein energy

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Macromolecule #49: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,...

MacromoleculeName: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL
type: ligand / ID: 49 / Number of copies: 4 / Formula: PLX
Molecular weightTheoretical: 767.132 Da
Chemical component information

ChemComp-PLX:
(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / phospholipid*YM

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Macromolecule #50: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate
type: ligand / ID: 50 / Number of copies: 2 / Formula: 8Q1
Molecular weightTheoretical: 540.651 Da
Chemical component information

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

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Macromolecule #51: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 51 / Number of copies: 6 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 52 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

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Macromolecule #53: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 53 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

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Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #55: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 55 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #56: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer

MacromoleculeName: Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer / type: ligand / ID: 56 / Number of copies: 1 / Formula: UQ
Molecular weightTheoretical: 863.343 Da
Chemical component information

ChemComp-UQ:
Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer

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Macromolecule #57: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.3 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: RANDOM ASSIGNMENT
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 53905

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