+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-30305 | |||||||||
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Title | Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD | |||||||||
Map data | Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD | |||||||||
Sample |
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Function / homology | Function and homology information angiotensin-converting enzyme 2 / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / peptidyl-dipeptidase activity / carboxypeptidase activity / metallopeptidase activity / virus receptor activity / Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release ...angiotensin-converting enzyme 2 / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / peptidyl-dipeptidase activity / carboxypeptidase activity / metallopeptidase activity / virus receptor activity / Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / membrane => GO:0016020 / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / membrane fusion / Attachment and Entry / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / symbiont-mediated suppression of host innate immune response / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / cell surface / extracellular space / identical protein binding / membrane / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Felis catus (domestic cat) / Severe acute respiratory syndrome coronavirus 2 | |||||||||
Method | single particle reconstruction / Resolution: 3.0 Å | |||||||||
Authors | Gao GF / Wang QH / Wu L | |||||||||
Funding support | China, 1 items
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Citation | Journal: Cell Discov / Year: 2020 Title: Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2. Authors: Lili Wu / Qian Chen / Kefang Liu / Jia Wang / Pengcheng Han / Yanfang Zhang / Yu Hu / Yumin Meng / Xiaoqian Pan / Chengpeng Qiao / Siyu Tian / Pei Du / Hao Song / Weifeng Shi / Jianxun Qi / ...Authors: Lili Wu / Qian Chen / Kefang Liu / Jia Wang / Pengcheng Han / Yanfang Zhang / Yu Hu / Yumin Meng / Xiaoqian Pan / Chengpeng Qiao / Siyu Tian / Pei Du / Hao Song / Weifeng Shi / Jianxun Qi / Hong-Wei Wang / Jinghua Yan / George Fu Gao / Qihui Wang / Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the recent pandemic COVID-19, is reported to have originated from bats, with its intermediate host unknown to date. ...Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the recent pandemic COVID-19, is reported to have originated from bats, with its intermediate host unknown to date. Here, we screened 26 animal counterparts of the human ACE2 (hACE2), the receptor for SARS-CoV-2 and SARS-CoV, and found that the ACE2s from various species, including pets, domestic animals and multiple wild animals, could bind to SARS-CoV-2 receptor binding domain (RBD) and facilitate the transduction of SARS-CoV-2 pseudovirus. Comparing to SARS-CoV-2, SARS-CoV seems to have a slightly wider range in choosing its receptor. We further resolved the cryo-electron microscopy (cryo-EM) structure of the cat ACE2 (cACE2) in complex with the SARS-CoV-2 RBD at a resolution of 3 Å, revealing similar binding mode as hACE2 to the SARS-CoV-2 RBD. These results shed light on pursuing the intermediate host of SARS-CoV-2 and highlight the necessity of monitoring susceptible hosts to prevent further outbreaks. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_30305.map.gz | 2.5 MB | EMDB map data format | |
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Header (meta data) | emd-30305-v30.xml emd-30305.xml | 12.6 KB 12.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_30305_fsc.xml | 6.5 KB | Display | FSC data file |
Images | emd_30305.png | 62.1 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-30305 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30305 | HTTPS FTP |
-Validation report
Summary document | emd_30305_validation.pdf.gz | 367.8 KB | Display | EMDB validaton report |
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Full document | emd_30305_full_validation.pdf.gz | 367.4 KB | Display | |
Data in XML | emd_30305_validation.xml.gz | 9.2 KB | Display | |
Data in CIF | emd_30305_validation.cif.gz | 11.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30305 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30305 | HTTPS FTP |
-Related structure data
Related structure data | 7c8dMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_30305.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.99375 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD
Entire | Name: Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD |
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Components |
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-Supramolecule #1: Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD
Supramolecule | Name: Cryo-EM structure of cat ACE2 and SARS-CoV-2 RBD / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: Felis catus (domestic cat) |
-Supramolecule #2: cat ACE2
Supramolecule | Name: cat ACE2 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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-Supramolecule #3: SARS-CoV-2 RBD
Supramolecule | Name: SARS-CoV-2 RBD / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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-Macromolecule #1: Angiotensin-converting enzyme 2
Macromolecule | Name: Angiotensin-converting enzyme 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: angiotensin-converting enzyme 2 |
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Source (natural) | Organism: Felis catus (domestic cat) |
Molecular weight | Theoretical: 85.132953 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MQSTTEELAK TFLEKFNHEA EELSYQSSLA SWNYNTNITD ENVQKMNEAG AKWSAFYEEQ SKLAKTYPLA EIHNTTVKRQ LQALQQSGS SVLSADKSQR LNTILNAMST IYSTGKACNP NNPQECLLLE PGLDDIMENS KDYNERLWAW EGWRAEVGKQ L RPLYEEYV ...String: MQSTTEELAK TFLEKFNHEA EELSYQSSLA SWNYNTNITD ENVQKMNEAG AKWSAFYEEQ SKLAKTYPLA EIHNTTVKRQ LQALQQSGS SVLSADKSQR LNTILNAMST IYSTGKACNP NNPQECLLLE PGLDDIMENS KDYNERLWAW EGWRAEVGKQ L RPLYEEYV ALKNEMARAN NYEDYGDYWR GDYEEEWTDG YNYSRSQLIK DVEHTFTQIK PLYQHLHAYV RAKLMDTYPS RI SPTGCLP AHLLGDMWGR FWTNLYPLTV PFGQKPNIDV TDAMVNQSWD ARRIFKEAEK FFVSVGLPNM TQGFWENSML TEP GDSRKV VCHPTAWDLG KGDFRIKMCT KVTMDDFLTA HHEMGHIQYD MAYAVQPFLL RNGANEGFHE AVGEIMSLSA ATPN HLKTI GLLSPGFSED SETEINFLLK QALTIVGTLP FTYMLEKWRW MVFKGEIPKE QWMQKWWEMK REIVGVVEPV PHDET YCDP ASLFHVANDY SFIRYYTRTI YQFQFQEALC RIAKHEGPLH KCDISNSSEA GKKLLQMLTL GKSKPWTLAL EHVVGE KKM NVTPLLKYFE PLFTWLKEQN RNSFVGWNTD WRPYADQSIK VRISLKSALG DEAYEWNDNE MYLFRSSVAY AMREYFS KV KNQTIPFVED NVWVSNLKPR ISFNFFVTAS KNVSDVIPRS EVEEAIRMSR SRINDAFRLD DNSLEFLGIQ PTLSPPYQ P PVTHHHHHHH H |
-Macromolecule #2: Spike protein S1
Macromolecule | Name: Spike protein S1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Molecular weight | Theoretical: 21.873496 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADY NYKLPDDFTG CVIAWNSNNL DSKVGGNYNY LYRLFRKSNL KPFERDISTE IYQAGSTPCN GVEGFNCYFP L QSYGFQPT ...String: TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADY NYKLPDDFTG CVIAWNSNNL DSKVGGNYNY LYRLFRKSNL KPFERDISTE IYQAGSTPCN GVEGFNCYFP L QSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGP |
-Macromolecule #3: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 3 / Number of copies: 1 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Processing | single particle reconstruction |
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Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | #0 - Image recording ID: 1 / #0 - Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / #0 - Average electron dose: 50.0 e/Å2 / #1 - Image recording ID: 2 / #1 - Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / #1 - Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |