response to salt stress / response to hydrogen peroxide / protein homooligomerization / : / unfolded protein binding / protein folding / protein complex oligomerization / response to heat / cytoplasm 類似検索 - 分子機能
: / Hsp20/alpha crystallin family / Small heat shock protein (sHSP) domain profile. / Alpha crystallin/Hsp20 domain / HSP20-like chaperone 類似検索 - ドメイン・相同性
ジャーナル: Structure / 年: 2006 タイトル: Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. 著者: Helen E White / Elena V Orlova / Shaoxia Chen / Luchun Wang / Athanasios Ignatiou / Brent Gowen / Thusnelda Stromer / Titus M Franzmann / Martin Haslbeck / Johannes Buchner / Helen R Saibil / 要旨: Small heat shock proteins are a superfamily of molecular chaperones that suppress protein aggregation and provide protection from cell stress. A key issue for understanding their action is to define ...Small heat shock proteins are a superfamily of molecular chaperones that suppress protein aggregation and provide protection from cell stress. A key issue for understanding their action is to define the interactions of subunit domains in these oligomeric assemblies. Cryo-electron microscopy of yeast Hsp26 reveals two distinct forms, each comprising 24 subunits arranged in a porous shell with tetrahedral symmetry. The subunits form elongated, asymmetric dimers that assemble via trimeric contacts. Modifications of both termini cause rearrangements that yield a further four assemblies. Each subunit contains an N-terminal region, a globular middle domain, the alpha-crystallin domain, and a C-terminal tail. Twelve of the C termini form 3-fold assembly contacts which are inserted into the interior of the shell, while the other 12 C termini form contacts on the surface. Hinge points between the domains allow a variety of assembly contacts, providing the flexibility required for formation of supercomplexes with non-native proteins.
履歴
登録
2006年5月25日
登録サイト: RCSB / 処理サイト: RCSB
改定 1.0
2006年8月1日
Provider: repository / タイプ: Initial release
改定 1.1
2008年5月1日
Group: Version format compliance
改定 1.2
2011年7月13日
Group: Derived calculations / Version format compliance
SEQUENCE THE SEQUENCE LISTED HERE IS THAT OF HEAT SHOCK PROTEIN 16.9B CORRESPONDING TO THE PDB ...SEQUENCE THE SEQUENCE LISTED HERE IS THAT OF HEAT SHOCK PROTEIN 16.9B CORRESPONDING TO THE PDB ENTRY 1GME. THE ACTUAL SEQUENCE IN THE SAMPLE IS THAT OF UNIPROT ENTRY P15992.
A: small heat shock protein Hsp26 B: small heat shock protein Hsp26 C: small heat shock protein Hsp26 D: small heat shock protein Hsp26 E: small heat shock protein Hsp26 F: small heat shock protein Hsp26 G: small heat shock protein Hsp26 H: small heat shock protein Hsp26 I: small heat shock protein Hsp26 J: small heat shock protein Hsp26 K: small heat shock protein Hsp26 L: small heat shock protein Hsp26 M: small heat shock protein Hsp26 N: small heat shock protein Hsp26 O: small heat shock protein Hsp26 P: small heat shock protein Hsp26 Q: small heat shock protein Hsp26 R: small heat shock protein Hsp26 S: small heat shock protein Hsp26 T: small heat shock protein Hsp26 U: small heat shock protein Hsp26 V: small heat shock protein Hsp26 W: small heat shock protein Hsp26 X: small heat shock protein Hsp26