- PDB-1gme: Crystal structure and assembly of an eukaryotic small heat shock ... -
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Basic information
Entry
Database: PDB / ID: 1gme
Title
Crystal structure and assembly of an eukaryotic small heat shock protein
Components
HEAT SHOCK PROTEIN 16.9B
Keywords
CHAPERONE / SMALL HEAT SHOCK PROTEIN / ALPHA-CRYSTALLIN
Function / homology
Function and homology information
response to salt stress / response to hydrogen peroxide / protein homooligomerization / : / unfolded protein binding / protein folding / protein complex oligomerization / response to heat / cytoplasm Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 33 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE SEQUENCE SHOWS SER AT POSITION 7 INSTEAD OF THR AS REPORTED IN THE SWISSPROT ENTRY. SUBSEQUENT ...THE SEQUENCE SHOWS SER AT POSITION 7 INSTEAD OF THR AS REPORTED IN THE SWISSPROT ENTRY. SUBSEQUENT RESEQUENCING HAS CONFIRMED A SERINE RESIDUE AT POSITION 7
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.59 Å3/Da / Density % sol: 52 % / Description: MAD DATA COLLECTED ON BM14
Crystal grow
pH: 8.5 Details: PROTEIN WAS CRYSTALLIZED FROM 26-29% PEG400, 0.2M SODIUM CITRATE, 0.1M TRIS/HCL PH8.5, pH 8.50
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