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- EMDB-23658: Canine parvovirus and Fab14 asymmetric reconstruction -

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Basic information

Entry
Database: EMDB / ID: EMD-23658
TitleCanine parvovirus and Fab14 asymmetric reconstruction
Map dataCanine parvovirus and Fab14 asymmetric reconstruction
Sample
  • Complex: Canine parvovirus and FAB
    • Complex: FAB
      • Protein or peptide: CPV Fab14 light chain
      • Protein or peptide: CPV Fab14 heavy chain
    • Complex: Canine parvovirus
      • Protein or peptide: Capsid protein 2
Keywordscanine parvovirus / CPV / Fab14 / VIRUS / VIRUS-Immune System complex
Function / homology
Function and homology information


symbiont entry into host cell via permeabilization of host membrane / T=1 icosahedral viral capsid / clathrin-dependent endocytosis of virus by host cell / virion attachment to host cell / structural molecule activity
Similarity search - Function
Parvovirus coat protein VP2 / Parvovirus coat protein VP1/VP2 / Parvovirus coat protein VP2 / Capsid/spike protein, ssDNA virus
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse) / Canine parvovirus / Canine parvovirus type 2
Methodsingle particle reconstruction / cryo EM / Resolution: 2.4 Å
AuthorsGoteschius DJ / Hartmann SR
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/Office of the DirectorS10RR031780 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site.
Authors: Daniel J Goetschius / Samantha R Hartmann / Lindsey J Organtini / Heather Callaway / Kai Huang / Carol M Bator / Robert E Ashley / Alexander M Makhov / James F Conway / Colin R Parrish / Susan L Hafenstein /
Abstract: Canine parvovirus is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis, and cerebellar disease. Overlap on the surface of parvovirus capsids ...Canine parvovirus is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis, and cerebellar disease. Overlap on the surface of parvovirus capsids between the antigenic epitope and the receptor binding site has contributed to cross-species transmission, giving rise to closely related variants. It has been shown that Mab 14 strongly binds and neutralizes canine but not feline parvovirus, suggesting this antigenic site also controls species-specific receptor binding. To visualize the conformational epitope at high resolution, we solved the cryogenic electron microscopy (cryo-EM) structure of the Fab-virus complex. We also created custom software, Icosahedral Subparticle Extraction and Correlated Classification, to solve a Fab-virus complex with only a few Fab bound per capsid and visualize local structures of the Fab-bound and -unbound antigenic sites extracted from the same complex map. Our results identified the antigenic epitope that had significant overlap with the receptor binding site, and the structures revealed that binding of Fab induced conformational changes to the virus. We were also able to assign the order and position of attached Fabs to allow assessment of complementarity between the Fabs bound to different positions. This approach therefore provides a method for using cryo-EM to investigate complementarity of antibody binding.
History
DepositionMar 18, 2021-
Header (metadata) releaseJul 28, 2021-
Map releaseJul 28, 2021-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.2
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.2
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7m3n
  • Surface level: 0.2
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7m3n
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_23658.png

Downloads & links

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Map

FileDownload / File: emd_23658.map.gz / Format: CCP4 / Size: 371.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCanine parvovirus and Fab14 asymmetric reconstruction
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 460 pix.
= 506. Å		1.1 Å/pix.
x 460 pix.
= 506. Å		1.1 Å/pix.
x 460 pix.
= 506. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2 / Movie #1: 0.2
Minimum - Maximum-0.0328118 - 1.5686238
Average (Standard dev.)0.0045678513 (±0.04654274)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-230-230-230
Dimensions460460460
Spacing460460460
CellA=B=C: 506.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z460460460
origin x/y/z0.0000.0000.000
length x/y/z506.000506.000506.000
α/β/γ90.00090.00090.000
start NX/NY/NZ1331310
NX/NY/NZ223226424
MAP C/R/S123
start NC/NR/NS-230-230-230
NC/NR/NS460460460
D min/max/mean-0.0331.5690.005

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Supplemental data

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Half map: Half-map 1

Fileemd_23658_half_map_1.map
AnnotationHalf-map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map 2

Fileemd_23658_half_map_2.map
AnnotationHalf-map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Canine parvovirus and FAB

EntireName: Canine parvovirus and FAB
Components
  • Complex: Canine parvovirus and FAB
    • Complex: FAB
      • Protein or peptide: CPV Fab14 light chain
      • Protein or peptide: CPV Fab14 heavy chain
    • Complex: Canine parvovirus
      • Protein or peptide: Capsid protein 2

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Supramolecule #1: Canine parvovirus and FAB

SupramoleculeName: Canine parvovirus and FAB / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all

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Supramolecule #2: FAB

SupramoleculeName: FAB / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2
Source (natural)Organism: Mus musculus (house mouse)

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Supramolecule #3: Canine parvovirus

SupramoleculeName: Canine parvovirus / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Canine parvovirus

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Macromolecule #1: CPV Fab14 light chain

MacromoleculeName: CPV Fab14 light chain / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 11.981362 KDa
SequenceString:
DIVMTQSHKF MSTSVGDRVS ITCKASQDVN TALAWYQQIP GQSPKLLIYS ASNRYTGVPD RFTASGSGTD FTFTISSVQA EDLALYYCQ QHYTTPWTFG GGTKLEIKR

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Macromolecule #2: CPV Fab14 heavy chain

MacromoleculeName: CPV Fab14 heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 13.10453 KDa
SequenceString:
AVHLQGTELV KPGASAGVKL SCKASGYTFT NYDMNWVRQR PEQGLEWIGW IFPGDGSTRY NEKFKGKATL TTDKSSSTAY QLNRLTSED SAVYFCARRG SHGSYSFAYW GQGTLVTVSG

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Macromolecule #3: Capsid protein 2

MacromoleculeName: Capsid protein 2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Canine parvovirus type 2
Molecular weightTheoretical: 64.705559 KDa
SequenceString: MSDGAVQPDG GQPAVRNERA TGSGNGSGGG GGGGSGGVGI STGTFNNQTE FKFLENGWVE ITANSSRLVH LNMPESENYR RVVVNNMDK TAVNGNMALD DIHAQIVTPW SLVDANAWGV WFNPGDWQLI VNTMSELHLV SFEQEIFNVV LKTVSESATQ P PTKVYNND ...String:
MSDGAVQPDG GQPAVRNERA TGSGNGSGGG GGGGSGGVGI STGTFNNQTE FKFLENGWVE ITANSSRLVH LNMPESENYR RVVVNNMDK TAVNGNMALD DIHAQIVTPW SLVDANAWGV WFNPGDWQLI VNTMSELHLV SFEQEIFNVV LKTVSESATQ P PTKVYNND LTASLMVALD SNNTMPFTPA AMRSETLGFY PWKPTIPTPW RYYFQWDRTL IPSHTGTSGT PTNIYHGTDP DD VQFYTIE NSVPVHLLRT GDEFATGTFF FDCKPCRLTH TWQTNRALGL PPFLNSLPQS EGATNFGDIG VQQDKRRGVT QMG NTNYIT EATIMRPAEV GYSAPYYSFE ASTQGPFKTP IAAGRGGAQT DENQAADGNP RYAFGRQHGQ KTTTTGETPE RFTY IAHQD TGRYPEGDWI QNINFNLPVT NDNVLLPTDP IGGKTGINYT NIFNTYGPLT ALNNVPPVYP NGQIWDKEFD TDLKP RLHV NAPFVCQNNC PGQLFVKVAP NLTNEYDPDA SANMSRIVTY SDFWWKGKLV FKAKLRASHT WNPIQQMSIN VDNQFN YVP SNIGGMKIVY EKSQLAPRKL Y

UniProtKB: Capsid protein 2

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF
Details: Asymmetric reconstruction, particle number is 162,627*10.2 orientations per particle
Number images used: 162627
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: OTHER

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