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- EMDB-23028: Negative stain EM structure of the human SAGA coactivator complex... -

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基本情報

登録情報
データベース: EMDB / ID: EMD-23028
タイトルNegative stain EM structure of the human SAGA coactivator complex (TRRAP, core, splicing module)
マップデータmap
試料
  • 複合体: human SAGA
    • 複合体: human SAGA
      • タンパク質・ペプチド: x 12種
    • 複合体: STAGA complex 65 subunit gamma
      • タンパク質・ペプチド: x 1種
キーワードsplicing / gene regulation (遺伝子発現の調節) / transcription (転写 (生物学)) / chromatin (クロマチン)
機能・相同性
機能・相同性情報


regulation of macromolecule metabolic process / : / regulation of primary metabolic process / SAGA-type complex / positive regulation of response to stimulus / regulation of somatic stem cell population maintenance / SAGA complex assembly / lateral mesodermal cell differentiation / allantois development / regulation of cellular response to stress ...regulation of macromolecule metabolic process / : / regulation of primary metabolic process / SAGA-type complex / positive regulation of response to stimulus / regulation of somatic stem cell population maintenance / SAGA complex assembly / lateral mesodermal cell differentiation / allantois development / regulation of cellular response to stress / transcription factor TFTC complex / SLIK (SAGA-like) complex / splicing factor binding / U12-type spliceosomal complex / negative regulation of microtubule depolymerization / hepatocyte differentiation / maintenance of protein location in nucleus / RNA splicing, via transesterification reactions / U2-type spliceosomal complex / U2-type precatalytic spliceosome / U2-type prespliceosome assembly / RNA polymerase binding / SAGA complex / U2 snRNP / limb development / 転写開始前複合体 / precatalytic spliceosome / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / nucleus organization / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / mRNA Splicing - Minor Pathway / regulation of RNA splicing / histone deacetylase complex / transcription factor TFIID complex / RNA polymerase II general transcription initiation factor activity / histone acetyltransferase complex / positive regulation of transcription initiation by RNA polymerase II / embryonic placenta development / U2 snRNA binding / regulation of DNA repair / somitogenesis / gastrulation / RNA polymerase II preinitiation complex assembly / RNA Polymerase II Pre-transcription Events / 視覚 / TBP-class protein binding / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / male germ cell nucleus / DNA-templated transcription initiation / promoter-specific chromatin binding / nuclear estrogen receptor binding / transcription initiation at RNA polymerase II promoter / transcription coregulator activity / spliceosomal complex / mRNA transcription by RNA polymerase II / multicellular organism growth / negative regulation of protein catabolic process / G1/S transition of mitotic cell cycle / オートファジー / nuclear matrix / microtubule cytoskeleton organization / mRNA splicing, via spliceosome / cytoplasmic ribonucleoprotein granule / transcription corepressor activity / microtubule cytoskeleton / HATs acetylate histones / positive regulation of cell growth / DNA-binding transcription factor binding / Regulation of TP53 Activity through Phosphorylation / transcription by RNA polymerase II / transcription coactivator activity / protein stabilization / Ub-specific processing proteases / nuclear speck / クロマチンリモデリング / protein heterodimerization activity / focal adhesion / apoptotic process / protein-containing complex binding / regulation of DNA-templated transcription / 核小体 / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / ゴルジ体 / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / extracellular exosome
類似検索 - 分子機能
STAGA complex 65 subunit gamma / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region ...STAGA complex 65 subunit gamma / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / Bromodomain associated / Bromodomain transcription factors and PHD domain containing proteins / Bromodomain associated domain / Transcription initiation factor IID, 31kD subunit / Splicing factor 3B, subunit 5 / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TAF6, C-terminal HEAT repeat domain superfamily / TATA box binding protein associated factor (TAF) / Transcription initiation factor TFIID subunit 12 domain / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Cleavage/polyadenylation specificity factor, A subunit, N-terminal / Mono-functional DNA-alkylating methyl methanesulfonate N-term / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / CPSF A subunit region / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Histone-fold / Armadillo-like helical / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40リピート / WD40リピート / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Protein kinase-like domain superfamily
類似検索 - ドメイン・相同性
Transformation/transcription domain associated protein / Ataxin-7 / Transcription initiation protein SPT3 homolog / TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L / STAGA complex 65 subunit gamma / Transcription initiation factor TFIID subunit 10 / Splicing factor 3B subunit 3 / Transcription initiation factor TFIID subunit 12 / Transcription factor SPT20 homolog / Transcriptional adapter 1 ...Transformation/transcription domain associated protein / Ataxin-7 / Transcription initiation protein SPT3 homolog / TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L / STAGA complex 65 subunit gamma / Transcription initiation factor TFIID subunit 10 / Splicing factor 3B subunit 3 / Transcription initiation factor TFIID subunit 12 / Transcription factor SPT20 homolog / Transcriptional adapter 1 / Splicing factor 3B subunit 5 / Transcription initiation factor TFIID subunit 9B / TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
類似検索 - 構成要素
生物種Homo sapiens (ヒト) / unclassified Rhodococcus (バクテリア)
手法単粒子再構成法 / ネガティブ染色法 / 解像度: 19.09 Å
データ登録者Herbst DA / Esbin MN
資金援助 米国, European Union, スイス, 6件
OrganizationGrant number国
Howard Hughes Medical Institute (HHMI)CC30250 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM127018 米国
European Molecular Biology Organization (EMBO)ALTF 1002-2018European Union
Swiss National Science FoundationP2BSP3_181878 スイス
Howard Hughes Medical Institute (HHMI)CC34430 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32GM098218 米国
引用ジャーナル: Nat Struct Mol Biol / 年: 2021
タイトル: Structure of the human SAGA coactivator complex.
著者: Dominik A Herbst / Meagan N Esbin / Robert K Louder / Claire Dugast-Darzacq / Gina M Dailey / Qianglin Fang / Xavier Darzacq / Robert Tjian / Eva Nogales /
要旨: The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are ...The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are noteworthy functional and compositional differences for this complex in metazoans. Here we present the cryogenic-electron microscopy (cryo-EM) structure of human SAGA (hSAGA) and show how the arrangement of distinct structural elements results in a globally divergent organization from that of yeast, with a different interface tethering the core module to the TRRAP subunit, resulting in a dramatically altered geometry of functional elements and with the integration of a metazoan-specific splicing module. Our hSAGA structure reveals the presence of an inositol hexakisphosphate (InsP) binding site in TRRAP and an unusual property of its pseudo-(Ψ)PIKK. Finally, we map human disease mutations, thus providing the needed framework for structure-guided drug design of this important therapeutic target for human developmental diseases and cancer.
履歴
登録2020年11月24日-
ヘッダ(付随情報) 公開2021年11月10日-
マップ公開2021年11月10日-
更新2024年5月29日-
現状2024年5月29日処理サイト: RCSB / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.0302
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.0302
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-7kts
  • 表面レベル: 0.0302
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_23028.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_23028.map.gz / 形式: CCP4 / 大きさ: 103 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈map
ボクセルのサイズX=Y=Z: 1.4 Å
密度
表面レベル登録者による: 0.0302 / ムービー #1: 0.0302
最小 - 最大-0.027611308 - 0.065977685
平均 (標準偏差)0.00026056074 (±0.006214624)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ300300300
Spacing300300300
セルA=B=C: 420.0 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.41.41.4
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z420.000420.000420.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.0280.0660.000

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添付データ

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マスク #1

ファイルemd_23028_msk_1.map
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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ハーフマップ: half map 1

ファイルemd_23028_half_map_1.map
注釈half map 1
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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ハーフマップ: half map 2

ファイルemd_23028_half_map_2.map
注釈half map 2
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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試料の構成要素

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全体 : human SAGA

全体名称: human SAGA
要素
  • 複合体: human SAGA
    • 複合体: human SAGA
      • タンパク質・ペプチド: Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein
      • タンパク質・ペプチド: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
      • タンパク質・ペプチド: Isoform 3 of Transcription factor SPT20 homolog
      • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 9B
      • タンパク質・ペプチド: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
      • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 12
      • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 10
      • タンパク質・ペプチド: Transcription initiation protein SPT3 homolog,Transcription initiation protein SPT3 homolog,Transcription initiation protein SPT3 homolog転写 (生物学)
      • タンパク質・ペプチド: Transcriptional adapter 1
      • タンパク質・ペプチド: Ataxin-7Ataxin 7
      • タンパク質・ペプチド: Splicing factor 3B subunit 3
      • タンパク質・ペプチド: Splicing factor 3B subunit 5
    • 複合体: STAGA complex 65 subunit gamma
      • タンパク質・ペプチド: STAGA complex 65 subunit gamma, DhaA,STAGA complex 65 subunit gamma,STAGA complex 65 subunit gamma, DhaA,STAGA complex 65 subunit gamma,STAGA complex 65 subunit gamma, DhaA

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超分子 #1: human SAGA

超分子名称: human SAGA / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all / 詳細: endogeneous human SAGA purified from HeLa cells
分子量理論値: 1.420272 MDa

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超分子 #2: human SAGA

超分子名称: human SAGA / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1-#3, #5-#13 / 詳細: endogeneous human SAGA purified from HeLa cells
由来(天然)生物種: Homo sapiens (ヒト) / 株: HeLa / 組織: cervix

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超分子 #3: STAGA complex 65 subunit gamma

超分子名称: STAGA complex 65 subunit gamma / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #4
由来(天然)生物種: Homo sapiens (ヒト) / 株: HeLa / 組織: cervix

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分子 #1: Transformation/transcription domain-associated protein,Transforma...

分子名称: Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated ...名称: Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein,Transformation/transcription domain-associated protein
タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 419.168094 KDa
配列文字列: MAFVATQGAT VVDQTTLMKK YLQFVAALTD VNTPDETKLK MMQEVSENFE NVTSSPQYST FLEHIIPRFL TFLQDGEVQF LQEKPAQQL RKLVLEIIHR IPTNEHLRPH TKNVLSVMFR FLETENEENV LICLRIIIEL HKQFRPPITQ EIHHFLDFVK Q IYKELPKV ...文字列:
MAFVATQGAT VVDQTTLMKK YLQFVAALTD VNTPDETKLK MMQEVSENFE NVTSSPQYST FLEHIIPRFL TFLQDGEVQF LQEKPAQQL RKLVLEIIHR IPTNEHLRPH TKNVLSVMFR FLETENEENV LICLRIIIEL HKQFRPPITQ EIHHFLDFVK Q IYKELPKV VNRYFENPQV IPENTVPPPE MVGMITTIAV KVNPEREDSE TRTHSIIPRG SLSLKVLAEL PIIVVLMYQL YK LNIHNVV AEFVPLIMNT IAIQVSAQAR QHKLYNKELY ADFIAAQIKT LSFLAYIIRI YQELVTKYSQ QMVKGMLQLL SNC PAETAH LRKELLIAAK HILT(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) ETLRPL AYSTLADLVH HVRQHLPLSD LSLAVQLFAK NIDDESLPSS IQTMSCKLLL NLVDCIRSKS EQESGNGRDV LMRM LEVFV LKFHTIARYQ LSAIFKKCKP QSELGAVEAA LPGVPTAPAA PGPAPSPAPV PAPPPPPPPP PPATPVTPAP VPPFE KQGE KDKEDKQTFQ VTDCRSLVKT LVCGVKTITW GITSCKAPGA QFIPNKQLQP KETQIYIKLV KYAMQALDIY QVQIAG NGQ TYIRVANCQT VRMKEEKEVL EHFAGVFTMM NPLTFKEIFQ TTVPYMVERI SKNYALQIVA NSFLANPTTS ALFATIL VE YLLDRLPEMG SNVELSNLYL KLFKLVFGSV SLFAAENEQM LKPHLHKIVN SSMELAQTAK EPYNYFLLLR ALFRSIGG G SHDLLYQEFL PLLPNLLQGL NMLQSGLHKQ HMKDLFVELC LTVPVRLSSL LPYLPMLMDP LVSALNGSQT LVSQGLRTL ELCVDNLQPD FLYDHIQPVR AELMQALWRT LRNPADSISH VAYRVLGKFG GSNRKMLKES QKLHYVVTEV QGPSITVEFS DCKASLQLP MEKAIETALD CLKSANTEPY YRRQAWEVIK CFLVAMMSLE DNKHALYQLL AHPNFTEKTI PNVIISHRYK A QDTPARKT FEQALTGAFM SAVIKDLRPS ALPFVASLIR HYTMVAVAQQ CGPFLLPCYQ VGSQPSTAMF HSEENGSKGM DP LVLIDAI AICMAYEEKE LCKIGEVALA VIFDVASIIL GSKERACQLP LFSYIVERLC ACCYEQAWYA KLGGVVSIKF LME RLPLTW VLQNQQTFLK ALLFVMMDLT GEVSNGAVAM AKTTLEQLLM RCATPLKDEE RAEEIVAAQE KSFHHVTHDL VREV TSPNS TVRKQAMHSL QVLAQVTGKS VTVIMEPHKE VLQDMVPPKK HLLRHQPANA QIGLMEGNTF CTTLQPRLFT MDLNV VEHK VFYTELLNLC EAEDSALTKL PCYKSLPSLV PLRIAALNAL AACNYLPQSR EKIIAALFKA LNSTNSELQE AGEACM RKF LEGATIEVDQ IHTHMRPLLM MLGDYRSLTL NVVNRLTSVT RLFPNSFNDK FCDQMMQHLR KWMEVVVITH KGGQRSD GN EMKICSAIIN LFHLIPAAPQ TLVKPLLEVV MKTERAMLIE AGSPFREPLI KFLTRHPSQT VELFMMEATL NDPQWSRM F MSFLKHKDAR PLRDVLAANP NRFITLLLPG GAQTAVRPGS PSTSTMRLDL QFQAIKIISI IVKNDDSWLA SQHSLVSQL RRVWVSENFQ ERHRKENMAA TNWKEPKLLA YCLLNYCKRN 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(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)A GTSELVMLSL ELVKTRLAVM SMEM RKNFI QAILTSLIEK SPDAKILRAV VKIVEEWVKN NSPMAANQTP TLREKSILLV KMMTYIEKRF PEDLELNAQF LDLVN YVYR DETLSGSELT AKLEPAFLSG LRCAQPLIRA KFFEVFDNSM KRRVYERLLY VTCSQNWEAM GNHFWIKQCI ELLLAV CEK STPIGTSCQG AMLPSITNVI NLADSHDRAA FAMVTHVKQE PRERENSESK EEDVEIDIEL APGDQTSTPK TKELSEK DI GNQLHMLTNR HDKFLDTLRE VKTGALLSAF VQLCHISTTL AEKTWVQLFP RLWKILSDRQ QHALAGEISP FLCSGSHQ V QRDCQPSALN CFVEAMSQCV PPIPIRPCVL KYLGKTHNLW FRSTLMLEHQ AFEKGLSLQI KPKQTTEFYE QESITPPQQ EILDSLAELY SLLQEEDMWA GLWQKRCKYS ETATAIAYEQ HGFFEQAQES YEKAMDKAKK EHERSNASPA IFPEYQLWED HWIRCSKEL NQWEALTEYG QSKGHINPYL VLECAWRVSN WTAMKEALVQ VEVSCPKEMA WKVNMYRGYL AICHPEEQQL S FIERLVEM ASSLAIREWR RLPHVVSHVH TPLLQAAQQI IELQEAAQIN AGLQPTNLGR NNSLHDMKTV VKTWRNRLPI VS DDLSHWS SIFMWRQHHY QAIVTAYENS SQHDPSSNNA MLGVHASASA IIQYGKIARK QGLVNVALDI LSRIHTIPTV PIV DCFQKI RQQVKCYLQL AGVMGKNECM QGLEVIESTN LKYFTKEMTA EFYALKGMFL AQINKSEEAN KAFSAAVQMH DVLV KAWAM WGDYLENIFV KERQLHLGVS AITCYLHACR HQNESKSRKY LAKVLWLLSF DDDKNTLADA VDKYCIGVPP IQWLA WIPQ LLTCLVGSEG KLLLNLISQV GRVYPQAVYF PIRTLYLTLK IEQRERYKSD SGQQQPSSVG NQSHSASDPG PIRATA PMW RCSRIMHMQR ELHPTLLSSL EGIVDQMVWF RENWHEEVLR QLQQGLAKCY SVAFEKSGAV SDAKITPHTL NFVKKLV ST FGVGLENVSN VSTMFSSAAS ESLARRAQAT AQDPVFQKLK GQFTTDFDFS VPGSMKLHNL ISKLKKWIKI LEAKTKQL P KFFLIEEKCR FLSNFSAQTA EVEIPGEFLM PKPTHYYIKI ARFMPRVEIV QKHNTAARRL YIRGHNGKIY PYLVMNDAC LTESRREERV LQLLRLLNPC LEKRKETTKR HLFFTVPRVV AVSPQMRLVE DNPSSLSLVE IYKQRCAKKG IEHDNPISRY YDRLATVQA RGTQASHQVL RDILKEVQSN MVPRSMLKEW ALHTFPNATD YWTFRKMFTI QLALIGFAEF VLHLNRLNPE M LQIAQDTG KLNVAYFRFD INDATGDLDA NRPVPFRLTP NISEFLTTIG VSGPLTASMI AVARCFAQPN FKVDGILKTV LR DEIIAWH KKTQEDTSSP LSAAGQPENM DSQQLVSLVQ KAVTAIMTRL HNLAQFEGGE SKVNTLVAAA NSLDNLCRMD PAW HPWL

UniProtKB: Transformation/transcription domain associated protein, Transformation/transcription domain associated protein, Transformation/transcription domain associated protein

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分子 #2: TAF5-like RNA polymerase II p300/CBP-associated factor-associated...

分子名称: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L
タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 66.223047 KDa
配列文字列: MKRVRTEQIQ MAVSCYLKRR QYVDSDGPLK QGLRLSQTAE EMAANLTVQS ESGCANIVSA APCQAEPQQY EVQFGRLRNF LTDSDSQHS HEVMPLLYPL FVYLHLNLVQ NSPKSTVESF YSRFHGMFLQ NASQKDVIEQ LQTTQTIQDI LSNFKLRAFL D NKYVVRLQ ...文字列:
MKRVRTEQIQ MAVSCYLKRR QYVDSDGPLK QGLRLSQTAE EMAANLTVQS ESGCANIVSA APCQAEPQQY EVQFGRLRNF LTDSDSQHS HEVMPLLYPL FVYLHLNLVQ NSPKSTVESF YSRFHGMFLQ NASQKDVIEQ LQTTQTIQDI LSNFKLRAFL D NKYVVRLQ EDSYNYLIRY LQSDNNTALC KVLTLHIHLD VQPAKRTDYQ LYASGSSSRS ENNGLEPPDM PSPILQNEAA LE VLQESIK RVKDGPPSLT TICFYAFYNT EQLLNTAEIS PDSKLLAAGF DNSCIKLWSL RSKKLKSEPH QVDVSRIHLA CDI LEEEDD EDDNAGTEMK ILRGHCGPVY STRFLADSSG LLSCSEDMSI RYWDLGSFTN TVLYQGHAYP VWDLDISPYS LYFA SGSHD RTARLWSFDR TYPLRIYAGH LADVDCVKFH PNSNYLATGS TDKTVRLWSA QQGNSVRLFT GHRGPVLSLA FSPNG KYLA SAGEDQRLKL WDLASGTLYK ELRGHTDNIT SLTFSPDSGL IASASMDNSV RVWDIRNTYC SAPADGSSSE LVGVYT GQM SNVLSVQFMA CNLLLVTGIT QENQEH

UniProtKB: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L

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分子 #3: Isoform 3 of Transcription factor SPT20 homolog

分子名称: Isoform 3 of Transcription factor SPT20 homolog / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 88.12907 KDa
配列文字列: MQQALELALD RAEYVIESAR QRPPKRKYLS SGRKSVFQKL YDLYIEECEK EPEVKKLRRN VNLLEKLVMQ ETLSCLVVNL YPGNEGYSL MLRGKNGSDS ETIRLPYEEG ELLEYLDAEE LPPILVDLLE KSQVNIFHCG CVIAEIRDYR QSSNMKSPGY Q SRHILLRP ...文字列:
MQQALELALD RAEYVIESAR QRPPKRKYLS SGRKSVFQKL YDLYIEECEK EPEVKKLRRN VNLLEKLVMQ ETLSCLVVNL YPGNEGYSL MLRGKNGSDS ETIRLPYEEG ELLEYLDAEE LPPILVDLLE KSQVNIFHCG CVIAEIRDYR QSSNMKSPGY Q SRHILLRP TMQTLICDVH SITSDNHKWT QEDKLLLESQ LILATAEPLC LDPSIAVTCT ANRLLYNKQK MNTRPMKRCF KR YSRSSLN RQQDLSHCPP PPQLRLLDFL QKRKERKAGQ HYDLKISKAG NCVDMWKRSP CNLAIPSEVD VEKYAKVEKS IKS DDSQPT VWPAHDVKDD YVFECEAGTQ YQKTKLTILQ SLGDPLYYGK IQPCKADEES DSQMSPSHSS TDDHSNWFII GSKT DAERV VNQYQELVQN EAKCPVKMSH SSSGSASLSQ VSPGKETDQT ETVSVQSSVL GKGVKHRPPP IKLPSSSGNS SSGNY FTPQ QTSSFLKSPT PPPSSKPSSI PRKSSVDLNQ VSMLSPAALS PASSSQRSGT PKPSTPTPTP SSTPHPPDAQ SSTPST PSA TPTPQDSGFT PQPTLLTQFA QQQRSLSQAM PVTTIPLSTM VTSITPGTTA TQVMANSAGL NFINVVGSVC GAQALMS GS NPMLGCNTGA ITPAGINLSG LLPSGGLLPN ALPSAMQAAS QAGVPFGLKN TSSLRPLNLL QLPGGSLIFN TLQQQQQQ L SQFTPQQPQQ PTTCSPQQPG EQGSEQGSTS QEQALSAQQA AVINLTGVGS FMQSQAAAVA ILAASNGYGS SSSTNSSAT SSSAYRQPVK K

UniProtKB: Transcription factor SPT20 homolog

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分子 #4: STAGA complex 65 subunit gamma, DhaA,STAGA complex 65 subunit gam...

分子名称: STAGA complex 65 subunit gamma, DhaA,STAGA complex 65 subunit gamma,STAGA complex 65 subunit gamma, DhaA,STAGA complex 65 subunit gamma,STAGA complex 65 subunit gamma, DhaA
タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: unclassified Rhodococcus (バクテリア)
分子量理論値: 83.013664 KDa
組換発現生物種: Homo sapiens (ヒト)
配列文字列: MNLQRYWGEI PISSSQTNRS SFDLLPREFR LV(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)MLDIPSEP CSLTIHTIQL IQHNRRLRNL IA TAQAQN(UNK) (UNK)(UNK) ...文字列:
MNLQRYWGEI PISSSQTNRS SFDLLPREFR LV(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)MLDIPSEP CSLTIHTIQL IQHNRRLRNL IA TAQAQN(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)DCKNPNAP FQIRHSDPES DFYRGKGEPV TELSWHSCRQ LLYQAVATIL AHAGFDCANE SVLETLTDVA HEYCLKF TK LLRFAVDREA RLGQTPFPDV MEQVFHEVGI GSVLSLQKFW QHRIKDYHSY MLQISKQLSE EYERIVNPEK ATEDAKPV K IKEEPVSDIT FPVSEELEAD LASGDQSLPM GVLGAQSERF PSNLEVEASP QASSAEVNAS PLWNLAHVKM EPQESEEGN VSGHGVLGSD VFEEPMSGMS EAGIPQSPDD SDSSYGSHST DSLMGSSPVF NQRCKKRMRK IGTSGEDLYF QSGGSMAEIG TGFPFDPHY VEVLGERMHY VDVGPRDGTP VLFLHGNPTS SYVWRNIIPH VAPTHRCIAP DLIGMGKSDK PDLGYFFDDH V RFMDAFIE ALGLEEVVLV IHDWGSALGF HWAKRNPERV KGIAFMEFIR PIPTWDEWPE FARETFQAFR TTDVGRKLII DQ NVFIEGT LPMGVVRPLT EVEMDHYREP FLNPVDREPL WRFPNELPIA GEPANIVALV EEYMDWLHQS PVPKLLFWGT PGV LIPPAE AARLAKSLPN CKAVDIGPGL NLLQEDNPDL IGSEIARWLS TLEISGDYKD HDGDYKDHDI DYKDDDDKGS

UniProtKB: STAGA complex 65 subunit gamma, STAGA complex 65 subunit gamma

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分子 #5: Transcription initiation factor TFIID subunit 9B

分子名称: Transcription initiation factor TFIID subunit 9B / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 27.654861 KDa
配列文字列: MESGKMAPPK NAPRDALVMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKPNVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQK NQTPLPLIKP YAGPRLPPDR YCLTAPNYRL KSLIKKGPNQ GRLVPRLSVG AVSSKPTTPT I ATPQTVSV ...文字列:
MESGKMAPPK NAPRDALVMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKPNVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQK NQTPLPLIKP YAGPRLPPDR YCLTAPNYRL KSLIKKGPNQ GRLVPRLSVG AVSSKPTTPT I ATPQTVSV PNKVATPMSV TSQRFTVQIP PSQSTPVKPV PATTAVQNVL INPSMIGPKN ILITTNMVSS QNTANEANPL KR KHEDDDD NDIM

UniProtKB: Transcription initiation factor TFIID subunit 9B

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分子 #6: TAF6-like RNA polymerase II p300/CBP-associated factor-associated...

分子名称: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like ...名称: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L,TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 62.027703 KDa
配列文字列: MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR RKLTVEDFNR ALRWSSVEAV CGYGSQEAL PMRPAREGEL YFPEDREVNL VELALATNIP KGCAETAVRV HVSYLDGKGN LAPQGSVPSA VSSL(UNK) (UNK)(UNK) ...文字列:
MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR RKLTVEDFNR ALRWSSVEAV CGYGSQEAL PMRPAREGEL YFPEDREVNL VELALATNIP KGCAETAVRV HVSYLDGKGN LAPQGSVPSA VSSL(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)Q AAEPNRGGPG GRGCRRLDD LPWDSLLFQE SSSGGGAEPS FGSGLPLPPG GAGPEDPSLS VTLADIYREL YAFFGDSLAT RFGTGQPAPT APRPPGDKKE PAAAPDSVR KMPQLTASAI VSPHGDESPR GSGGGGPASA SGPAASESRP LPRVHRARGA PRQQGPGTGT RDVFQKSRFA P RGAPHFRF IIAGRQAGRR CRGRLFQTAF PAPYGPSPAS RYVQKLPMIG RTSRPARRWA LSDYSLYLPL

UniProtKB: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L

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分子 #7: Transcription initiation factor TFIID subunit 12

分子名称: Transcription initiation factor TFIID subunit 12 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 17.948467 KDa
配列文字列:
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIAD DFIESVVTAA CQLARHRKSS TLEVKDVQLH LERQWNMWIP GFGSEEIRPY KKACTTEAHK QRMALIRKTT K K

UniProtKB: Transcription initiation factor TFIID subunit 12

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分子 #8: Transcription initiation factor TFIID subunit 10

分子名称: Transcription initiation factor TFIID subunit 10 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 21.731248 KDa
配列文字列: MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN ...文字列:
MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN DALQHCKMKG TASGSSRSKS KDRKYTLTME DLTPALSEYG INVKKPHYFT

UniProtKB: Transcription initiation factor TFIID subunit 10

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分子 #9: Transcription initiation protein SPT3 homolog,Transcription initi...

分子名称: Transcription initiation protein SPT3 homolog,Transcription initiation protein SPT3 homolog,Transcription initiation protein SPT3 homolog
タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 35.4475 KDa
配列文字列: MNNTAASPMS TATSSSGRST GKSISFATEL QSMMYSLGDA RRPLHETAVL VEDVVHTQLI NLLQQAAEVS QLRGARVITP EDLLFLMRK DKKKLRRLLK YMFIRDYKSK IVKGIDEDDL LEDKLSGSNN ANKRQKIAQD FLNSIDQTGE LLAMFEDDEI D EVKQERME ...文字列:
MNNTAASPMS TATSSSGRST GKSISFATEL QSMMYSLGDA RRPLHETAVL VEDVVHTQLI NLLQQAAEVS QLRGARVITP EDLLFLMRK DKKKLRRLLK YMFIRDYKSK IVKGIDEDDL LEDKLSGSNN ANKRQKIAQD FLNSIDQTGE LLAMFEDDEI D EVKQERME RAERQ(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)CES RQLS FSKKA SKFRDWLDCS SMEIKPNVVA MEILAYLAYE TVAQLVDLAL LVRQDMVTKA GDPFSHAISA TFIQYHNSAE STAAC GVEA HSDAIQPCHI REAIRRYSHR IGPLSPFTNA YRRNGMAFLA C

UniProtKB: Transcription initiation protein SPT3 homolog, Transcription initiation protein SPT3 homolog

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分子 #10: Transcriptional adapter 1

分子名称: Transcriptional adapter 1 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 37.432531 KDa
配列文字列: MATFVSELEA AKKNLSEALG DNVKQYWANL KLWFKQKISK EEFDLEAHRL LTQDNVHSHN DFLLAILTRC QILVSTPDGA GSLPWPGGS AAKPGKPKGK KKLSSVRQKF DHRFQPQNPL SGAQQFVAKD PQDDDDLKLC SHTMMLPTRG QLEGRMIVTA Y EHGLDNVT ...文字列:
MATFVSELEA AKKNLSEALG DNVKQYWANL KLWFKQKISK EEFDLEAHRL LTQDNVHSHN DFLLAILTRC QILVSTPDGA GSLPWPGGS AAKPGKPKGK KKLSSVRQKF DHRFQPQNPL SGAQQFVAKD PQDDDDLKLC SHTMMLPTRG QLEGRMIVTA Y EHGLDNVT EEAVSAVVYA VENHLKDILT SVVSRRKAYR LRDGHFKYAF GSNVTPQPYL KNSVVAYNNL IESPPAFTAP CA GQNPASH PPPDDAEQQA ALLLACSGDT LPASLPPVNM YDLFEALQVH REVIPTHTVY ALNIERIITK LWHPNHEELQ QDK VHRQRL AAKEGLLLC

UniProtKB: Transcriptional adapter 1

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分子 #11: Ataxin-7

分子名称: Ataxin-7 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 95.597742 KDa
配列文字列: MSERAADDVR GEPRRAAAAA GGAAAAAARQ QQQQQQQQQP PPPQPQRQQH PPPPPRRTRP EDGGPGAAST SAAAMATVGE RRPLPSPEV MLGQSWNLWV EASKLPGKDG TELDESFKEF GKNREVMGLC REDMPIFGFC PAHDDFYLVV CNDCNQVVKP Q AFQSHYER ...文字列:
MSERAADDVR GEPRRAAAAA GGAAAAAARQ QQQQQQQQQP PPPQPQRQQH PPPPPRRTRP EDGGPGAAST SAAAMATVGE RRPLPSPEV MLGQSWNLWV EASKLPGKDG TELDESFKEF GKNREVMGLC REDMPIFGFC PAHDDFYLVV CNDCNQVVKP Q AFQSHYER RHSSSSKPPL AVPPTSVFSF FPSLSKSKGG SASGSNRSSS GGVLSASSSS SKLLKSPKEK LQLRGNTRPM HP IQQSRVP HGRIMTPSVK VEKIHPKMDG TLLKSAVGPT CPATVSSLVK PGLNCPSIPK PTLPSPGQIL NGKGLPAPPT LEK KPEDNS NNRKFLNKRL SEREFDPDIH CGVIDLDTKK PCTRSLTCKT HSLTQRRAVQ GRRKRFDVLL AEHKNKTREK ELIR HPDSQ QPPQPLRDPH PAPPRTSQEP HQNPHGVIPS ESKPFVASKP KPHTPSLPRP PGCPAQQGGS APIDPPPVHE SPHPP LPAT EPASRLSSEE GEGDDKEESV EKLDCHYSGH HPQPASFCTF GSRQIGRGYY VFDSRWNRLR CALNLMVEKH LNAQLW KKI PPVPSTTSPI STRIPHRTNS VPTSQCGVSY LAAATVSTSP VLLSSTCISP NSKSVPAHGT TLNAQPAASG AMDPVCS MQ SRQVSSSSSS PSTPSGLSSV PSSPMSRKPQ KLKSSKSLRP KESSGNSTNC QNASSSTSGG SGKKRKNSSP LLVHSSSS S SSSSSSSHSM ESFRKNCVAH SGPPYPSTVT SSHSIGLNCV TNKANAVNVR HDQSGRGPPT GSPAESIKRM SVMVNSSDS TLSLGPFIHQ SNELPVNSHG SFSHSHTPLD KLIGKKRKCS PSSSSINNSS SKPTKVAKVP AVNNVHMKHT GTIPGAQGLM NSSLLHQPK ARP

UniProtKB: Ataxin-7

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分子 #12: Splicing factor 3B subunit 3

分子名称: Splicing factor 3B subunit 3 / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 135.718844 KDa
配列文字列: MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY ...文字列:
MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY HVVGVDVGFE NPMFACLEMD YEEADNDPTG EAAANTQQTL TFYELDLGLN HVVRKYSEPL EEHGNFLITV PG GSDGPSG VLICSENYIT YKNFGDQPDI RCPIPRRRND LDDPERGMIF VCSATHKTKS MFFFLAQTEQ GDIFKITLET DED MVTEIR LKYFDTVPVA AAMCVLKTGF LFVASEFGNH YLYQIAHLGD DDEEPEFSSA MPLEEGDTFF FQPRPLKNLV LVDE LDSLS PILFCQIADL ANEDTPQLYV ACGRGPRSSL RVLRHGLEVS EMAVSELPGN PNAVWTVRRH IEDEFDAYII VSFVN ATLV LSIGETVEEV TDSGFLGTTP TLSCSLLGDD ALVQVYPDGI RHIRADKRVN EWKTPGKKTI VKCAVNQRQV VIALTG GEL VYFEMDPSGQ LNEYTERKEM SADVVCMSLA NVPPGEQRSR FLAVGLVDNT VRIISLDPSD CLQPLSMQAL PAQPESL CI VEMGGTEKQD ELGERGSIGF LYLNIGLQNG VLLRTVLDPV TGDLSDTRTR YLGSRPVKLF RVRMQGQEAV LAMSSRSW L SYSYQSRFHL TPLSYETLEF ASGFASEQCP EGIVAISTNT LRILALEKLG AVFNQVAFPL QYTPRKFVIH PESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAF SVAVCRFSNT GEDWYVLVGV AKDLILNPRS VAGGFVYTYK LVNNGEKLEF LHKTPVEEVP AAIAPFQGRV L IGVGKLLR VYDLGKKKLL RKCENKHIAN YISGIQTIGH RVIVSDVQES FIWVRYKRNE NQLIIFADDT YPRWVTTASL LD YDTVAGA DKFGNICVVR LPPNTNDEVD EDPTGNKALW DRGLLNGASQ KAEVIMNYHV GETVLSLQKT TLIPGGSESL VYT TLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRT PPEVS KKLEDIRTRY AF

UniProtKB: Splicing factor 3B subunit 3

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分子 #13: Splicing factor 3B subunit 5

分子名称: Splicing factor 3B subunit 5 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 10.149369 KDa
配列文字列:
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN

UniProtKB: Splicing factor 3B subunit 5

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実験情報

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構造解析

手法ネガティブ染色法
解析単粒子再構成法
試料の集合状態particle

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試料調製

緩衝液pH: 7.5
構成要素:
濃度名称式
25.0 mM4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
200.0 mMsodium chloride塩化ナトリウムNaCl塩化ナトリウム
0.2 mMEthylenediaminetetraacetic acidエチレンジアミン四酢酸
6.0 mMmagnesium chlorideMgCl2
3.0 % (w/v)D(+)trehalose

詳細: Buffers were freshly prepared and 0.22 um filtered.
染色タイプ: NEGATIVE / 材質: uranyl formate
グリッドモデル: Homemade / 材質: COPPER / メッシュ: 400 / 支持フィルム - 材質: CARBON / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec. / 前処理 - 雰囲気: AIR / 前処理 - 気圧: 101.325 kPa
詳細The sample was crosslinked with 1 mM bis(sulfosuccinimidyl)suberate (BS3) prior to freezing

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電子顕微鏡法

顕微鏡FEI TECNAI 20
電子線加速電圧: 120 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELDBright-field microscopy / Cs: 2.2 mm / 最大 デフォーカス（公称値）: 3.9 µm / 最小 デフォーカス（公称値）: 0.4 µm / 倍率（公称値）: 80000
試料ステージ試料ホルダーモデル: SIDE ENTRY, EUCENTRIC
撮影フィルム・検出器のモデル: GATAN ULTRASCAN 4000 (4k x 4k)
撮影したグリッド数: 1 / 実像数: 745 / 平均電子線量: 35.0 e/Å2

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画像解析

粒子像選択選択した数: 45790
初期モデルモデルのタイプ: INSILICO MODEL / In silico モデル: stochastic gradient descent
初期 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 3.1)
最終 3次元分類クラス数: 4 / 平均メンバー数/クラス: 992 / ソフトウェア - 名称: RELION (ver. 3.1)
最終 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 3.1)
最終 再構成使用したクラス数: 3 / アルゴリズム: FOURIER SPACE / 解像度のタイプ: BY AUTHOR / 解像度: 19.09 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3.1) / 使用した粒子像数: 3157
FSC曲線 (解像度の算出)

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原子モデル構築 1

初期モデル
PDB IDChain

5ife

chain_id: B, residue_range: 15-80, source_name: PDB, initial_model_type: experimental model

5ife

chain_id: A, residue_range: 1-1217, source_name: PDB, initial_model_type: experimental model
精密化空間: REAL / プロトコル: RIGID BODY FIT
得られたモデル

PDB-7kts:
Negative stain EM structure of the human SAGA coactivator complex (TRRAP, core, splicing module)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る