- EMDB-20732: GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - (... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-20732
タイトル
GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - (LBD-TMD-C3(AS) I) - with antagonist ZK200775, without NTD
マップデータ
GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - with antagonist ZK200775, without NTD
試料
複合体: GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS
複合体: Glutamate receptor 2
タンパク質・ペプチド: Glutamate receptor 2
複合体: Protein cornichon homolog 3
タンパク質・ペプチド: Protein cornichon homolog 3
機能・相同性
機能・相同性情報
Cargo concentration in the ER / COPII-mediated vesicle transport / localization within membrane / regulation of AMPA receptor activity / neurotransmitter receptor localization to postsynaptic specialization membrane / spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space ...Cargo concentration in the ER / COPII-mediated vesicle transport / localization within membrane / regulation of AMPA receptor activity / neurotransmitter receptor localization to postsynaptic specialization membrane / spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / channel regulator activity / ligand-gated monoatomic cation channel activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / cellular response to glycine / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / positive regulation of synaptic transmission / glutamate receptor binding / extracellular ligand-gated monoatomic ion channel activity / glutamate-gated calcium ion channel activity / glutamate-gated receptor activity / presynaptic active zone membrane / response to fungicide / vesicle-mediated transport / regulation of synaptic transmission, glutamatergic / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / dendrite membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / cytoskeletal protein binding / dendrite cytoplasm / ionotropic glutamate receptor signaling pathway / SNARE binding / regulation of membrane potential / dendritic shaft / synaptic membrane / synaptic transmission, glutamatergic / PDZ domain binding / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / protein tetramerization / postsynaptic density membrane / ionotropic glutamate receptor binding / modulation of chemical synaptic transmission / establishment of protein localization / Schaffer collateral - CA1 synapse / terminal bouton / receptor internalization / cerebral cortex development / synaptic vesicle membrane / synaptic vesicle / presynapse / signaling receptor activity / presynaptic membrane / amyloid-beta binding / growth cone / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / perikaryon / dendritic spine / postsynaptic density / neuron projection / axon / neuronal cell body / glutamatergic synapse / synapse / dendrite / protein-containing complex binding / protein kinase binding / cell surface / endoplasmic reticulum / protein-containing complex / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能
Cornichon / Cornichon, conserved site / Cornichon protein / Cornichon family signature. / Cornichon / Bacterial extracellular solute-binding proteins, family 3 / Solute-binding protein family 3/N-terminal domain of MltF / Ionotropic glutamate receptor, metazoa / Ligated ion channel L-glutamate- and glycine-binding site / : ...Cornichon / Cornichon, conserved site / Cornichon protein / Cornichon family signature. / Cornichon / Bacterial extracellular solute-binding proteins, family 3 / Solute-binding protein family 3/N-terminal domain of MltF / Ionotropic glutamate receptor, metazoa / Ligated ion channel L-glutamate- and glycine-binding site / : / Ligand-gated ion channel / Ionotropic glutamate receptor, L-glutamate and glycine-binding domain / Ligated ion channel L-glutamate- and glycine-binding site / Ionotropic glutamate receptor / Eukaryotic homologues of bacterial periplasmic substrate binding proteins. / Receptor, ligand binding region / Receptor family ligand binding region / Periplasmic binding protein-like I 類似検索 - ドメイン・相同性
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development
R01HD061543
米国
引用
ジャーナル: Science / 年: 2019 タイトル: Structures of the AMPA receptor in complex with its auxiliary subunit cornichon. 著者: Terunaga Nakagawa / 要旨: In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes ...In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes are critical for synaptic plasticity, learning, and memory. The two major categories of AMPAR auxiliary subunits are transmembrane AMPAR regulatory proteins (TARPs) and cornichon homologs (CNIHs); these subunits share little homology and play distinct roles in controlling ion channel gating and trafficking of AMPAR. Here, I report high-resolution cryo-electron microscopy structures of AMPAR in complex with CNIH3. Contrary to its predicted membrane topology, CNIH3 lacks an extracellular domain and instead contains four membrane-spanning helices. The protein-protein interaction interface that dictates channel modulation and the lipids surrounding the complex are revealed. These structures provide insights into the molecular mechanism for ion channel modulation and assembly of AMPAR/CNIH3 complexes.
ダウンロード / ファイル: emd_20732.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - with antagonist ZK200775, without NTD
ボクセルのサイズ
X=Y=Z: 1.066 Å
密度
表面レベル
登録者による: 0.018 / ムービー #1: 0.018
最小 - 最大
-0.05631211 - 0.09510806
平均 (標準偏差)
0.00010620647 (±0.002830746)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
360
360
360
Spacing
360
360
360
セル
A=B=C: 383.76 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.066
1.066
1.066
M x/y/z
360
360
360
origin x/y/z
0.000
0.000
0.000
length x/y/z
383.760
383.760
383.760
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
360
360
360
D min/max/mean
-0.056
0.095
0.000
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添付データ
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試料の構成要素
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全体 : GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS
全体
名称: GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS
要素
複合体: GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS
複合体: Glutamate receptor 2
タンパク質・ペプチド: Glutamate receptor 2
複合体: Protein cornichon homolog 3
タンパク質・ペプチド: Protein cornichon homolog 3
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超分子 #1: GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS
超分子
名称: GluA2 in complex with CNIH3 at 4:4 stoichiometry in AS タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: Bound to antagonist ZK200775 (MPQX). Focused classification and refinement of the LBD, TMD, and CNIH3. The NTD is not aligned. No symmetry imposed.