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- PDB-1phf: CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED CO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1phf | ||||||
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Title | CRYSTAL STRUCTURES OF METYRAPONE-AND PHENYLIMIDAZOLE-INHIBITED COMPLEXES OF CYTOCHROME P450-CAM | ||||||
![]() | CYTOCHROME P450-CAM | ||||||
![]() | OXIDOREDUCTASE(OXYGENASE) | ||||||
Function / homology | ![]() camphor 5-monooxygenase / camphor 5-monooxygenase activity / (+)-camphor catabolic process / cholest-4-en-3-one 26-monooxygenase activity / steroid hydroxylase activity / cholesterol catabolic process / iron ion binding / heme binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Poulos, T.L. | ||||||
![]() | ![]() Title: Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam. Authors: Poulos, T.L. / Howard, A.J. #1: ![]() Title: Inhibitor-Induced Conformational Change in Cytochrome P450-Cam Authors: Raag, R. / Li, H. / Jones, B.C. / Poulos, T.L. #2: ![]() Title: Crystal Structure of Substrate-Free Pseudomonas Putida Cytochrome P450 Authors: Poulos, T.L. / Finzel, B.C. / Howard, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98.1 KB | Display | ![]() |
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PDB format | ![]() | 75.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486 KB | Display | ![]() |
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Full document | ![]() | 501.2 KB | Display | |
Data in XML | ![]() | 12.1 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 89 IS A CIS PROLINE. / 2: RESIDUE 100 IS A CIS PROLINE. / 3: RESIDUE 106 IS A CIS PROLINE. / 4: SEE REMARK 8. |
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Components
#1: Protein | Mass: 46588.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-PIM / |
#4: Water | ChemComp-HOH / |
Nonpolymer details | SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION ON THE OCTAHEDRAL ...SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.16 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 12-15 ℃ / pH: 7 / Method: unknown / Details: P-450CAM was prepared in solutions containing 1 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
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Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.19 / Highest resolution: 1.6 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.6 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.6 Å / Rfactor obs: 0.19 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |