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- PDB-1ozg: The crystal structure of Klebsiella pneumoniae acetolactate synth... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ozg | ||||||
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Title | The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate | ||||||
![]() | Acetolactate synthase, catabolic | ||||||
![]() | LYASE / acetolactate synthase / acetohydroxyacid synthase / thiamin diphosphate | ||||||
Function / homology | ![]() butanediol metabolic process / acetolactate synthase / acetolactate synthase complex / acetolactate synthase activity / valine biosynthetic process / isoleucine biosynthetic process / thiamine pyrophosphate binding / flavin adenine dinucleotide binding / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pang, S.S. / Duggleby, R.G. / Schowen, R.L. / Guddat, L.W. | ||||||
![]() | ![]() Title: The Crystal Structures of Klebsiella pneumoniae Acetolactate Synthase with Enzyme-bound Cofactor and with an Unusual Intermediate. Authors: Pang, S.S. / Duggleby, R.G. / Schowen, R.L. / Guddat, L.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 235.1 KB | Display | ![]() |
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PDB format | ![]() | 185.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 48.7 KB | Display | |
Data in CIF | ![]() | 69.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1n0hC ![]() 1ozfC ![]() 1ozhC ![]() 1jscS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The biological assembly is a tetramer generated from the dimer in the asymmetric unit by the operation: -X,Y,-Z+1/2 |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 61355.766 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 626 molecules ![](data/chem/img/PO4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HE3.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HE3.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-PEG / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 48.86 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: PEG 8000, ethylene glycol, sodium HEPES , pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 17 ℃ / pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 22, 2002 / Details: mirrors |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→100 Å / Num. all: 48466 / Num. obs: 48466 / % possible obs: 88.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rsym value: 0.057 / Net I/σ(I): 17.7 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 1.7 % / Mean I/σ(I) obs: 4.5 / Num. unique all: 3030 / Rsym value: 0.126 / % possible all: 49.3 |
Reflection | *PLUS Lowest resolution: 100 Å / Num. measured all: 170273 / Rmerge(I) obs: 0.057 |
Reflection shell | *PLUS % possible obs: 49.3 % / Num. unique obs: 3030 / Num. measured obs: 5047 / Rmerge(I) obs: 0.126 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1JSC Resolution: 2.3→100 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 26.92 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→100 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.38 Å
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Refinement | *PLUS Lowest resolution: 100 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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