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- PDB-1m4g: Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tubercul... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1m4g | ||||||
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Title | Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Ribostamycin | ||||||
![]() | Aminoglycoside 2'-N-acetyltransferase | ||||||
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Function / homology | ![]() aminoglycoside 2'-N-acetyltransferase activity / aminoglycoside antibiotic catabolic process / aminoglycoside N-acetyltransferase activity / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Vetting, M.W. / Hegde, S.S. / Javid-Majd, F. / Blanchard, J.S. / Roderick, S.L. | ||||||
![]() | ![]() Title: Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates. Authors: Vetting, M.W. / Hegde, S.S. / Javid-Majd, F. / Blanchard, J.S. / Roderick, S.L. #1: ![]() Title: Overexpression and Mechanistic Analysis of Chromosomally Encoded Aminoglycoside 2'-N-Acetyltransferase (AAC(2')-Ic) from Mycobacterium tuberculosis Authors: Hegde, S.S. / Javid-Majd, F. / Blanchard, J.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.9 KB | Display | ![]() |
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PDB format | ![]() | 72.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1m44SC ![]() 1m4dC ![]() 1m4iC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a dimer as contained in the assymetric unit |
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Components
#1: Protein | Mass: 20063.699 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: P0A5N0, UniProt: P9WQG9*PLUS, ![]() #2: Chemical | ![]() #3: Chemical | ![]() #4: Chemical | #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.3 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Ammonium Sulfate, ADA, Coenzyme A, Ribostamycin, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Feb 2, 2002 / Details: Confocal Optics |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→24.09 Å / Num. all: 39240 / Num. obs: 38456 / % possible obs: 98 % / Observed criterion σ(I): -3 / Redundancy: 3.5 % / Biso Wilson estimate: 14.5 Å2 / Rsym value: 0.05 / Net I/σ(I): 16.5 |
Reflection shell | Resolution: 1.8→1.88 Å / Redundancy: 3.1 % / Mean I/σ(I) obs: 8.7 / Num. unique all: 4121 / Rsym value: 0.132 / % possible all: 96.3 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. obs: 38407 / % possible obs: 98.1 % / Rmerge(I) obs: 0.05 |
Reflection shell | *PLUS % possible obs: 96.3 % / Rmerge(I) obs: 0.132 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ID 1M44 Resolution: 1.8→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze | Luzzati coordinate error obs: 0.159 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.88 Å
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Refinement | *PLUS Lowest resolution: 50 Å / % reflection Rfree: 5 % / Rfactor Rfree![]() ![]() | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 2.1 | |||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor Rfree: 0.23 / Rfactor Rwork: 0.196 |