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Open data
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Basic information
Entry | Database: PDB / ID: 1kf6 | ||||||
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Title | E. coli Quinol-Fumarate Reductase with Bound Inhibitor HQNO | ||||||
![]() | (FUMARATE REDUCTASE ...) x 4 | ||||||
![]() | OXIDOREDUCTASE / respiration / fumarate reductace / succinate dehydrogenase / complex II / quinol / quinone | ||||||
Function / homology | ![]() : / succinate dehydrogenase activity / fermentation / fumarate metabolic process / succinate dehydrogenase / anaerobic electron transport chain / succinate dehydrogenase (quinone) activity / anaerobic respiration / 3 iron, 4 sulfur cluster binding / iron-sulfur cluster binding ...: / succinate dehydrogenase activity / fermentation / fumarate metabolic process / succinate dehydrogenase / anaerobic electron transport chain / succinate dehydrogenase (quinone) activity / anaerobic respiration / 3 iron, 4 sulfur cluster binding / iron-sulfur cluster binding / bacterial-type flagellum assembly / tricarboxylic acid cycle / FAD binding / 2 iron, 2 sulfur cluster binding / flavin adenine dinucleotide binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / DNA damage response / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Iverson, T.M. / Luna-Chavez, C. / Croal, L.R. / Cecchini, G. / Rees, D.C. | ||||||
![]() | ![]() Title: Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. Authors: Iverson, T.M. / Luna-Chavez, C. / Croal, L.R. / Cecchini, G. / Rees, D.C. #1: ![]() Title: Structure of the Escherichia coli Fumarate reductase respiratory complex Authors: Iverson, T.M. / Luna-Chavez, C. / Cecchini, G. / Rees, D.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 440.1 KB | Display | ![]() |
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PDB format | ![]() | 350.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 842.4 KB | Display | ![]() |
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Full document | ![]() | 927.3 KB | Display | |
Data in XML | ![]() | 56.4 KB | Display | |
Data in CIF | ![]() | 80.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1kfyC ![]() 1l0vC ![]() 1fum C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Components
-FUMARATE REDUCTASE ... , 4 types, 8 molecules AMBNCODP
#1: Protein | Mass: 66057.555 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 27021.885 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein | Mass: 14898.773 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #4: Protein | Mass: 13118.870 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 11 types, 39 molecules ![](data/chem/img/K.gif)
![](data/chem/img/OAA.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/FES.gif)
![](data/chem/img/F3S.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/HQO.gif)
![](data/chem/img/CE1.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/OAA.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/1PE.gif)
![](data/chem/img/FES.gif)
![](data/chem/img/F3S.gif)
![](data/chem/img/SF4.gif)
![](data/chem/img/HQO.gif)
![](data/chem/img/CE1.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Chemical | #7: Chemical | #8: Chemical | #9: Chemical | ChemComp-1PE / | #10: Chemical | #11: Chemical | #12: Chemical | #13: Chemical | #14: Chemical | ChemComp-CE1 / #15: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.75 Å3/Da / Density % sol: 67.23 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: PEG 5K MME, MgOAc, NaCitrate, EDTA, DTT, HQNO, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.8 | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 15, 1999 |
Radiation | Monochromator: 0.87 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→20 Å / Num. all: 292370 / Num. obs: 292370 / % possible obs: 91.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.7→2.74 Å / % possible all: 74 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. obs: 93174 / Num. measured all: 292370 / Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS % possible obs: 74 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 6.2 |
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Processing
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Refinement | Starting model: PDB entry 1FUM![]() 1fum Resolution: 2.7→20 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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Software | *PLUS Name: 'CNS AND REFMAC' / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 50 Å / σ(F): 0 / Rfactor obs: 0.231 / Rfactor Rfree: 0.28 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.0193 |