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- PDB-1i1h: CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX W... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1i1h | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE COMPLEX WITH HYDROGENOBYRINIC ACID | ||||||
![]() | PRECORRIN-8X METHYLMUTASE | ||||||
![]() | ISOMERASE / Precorrin / Vitamin B12 | ||||||
Function / homology | ![]() precorrin-8X methylmutase / precorrin-8X methylmutase activity / cobalamin biosynthetic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Shipman, L.W. / Li, D. / Roessner, C.A. / Scott, A.I. / Sacchettini, J.C. | ||||||
![]() | ![]() Title: Crystal structure of precorrin-8x methyl mutase. Authors: Shipman, L.W. / Li, D. / Roessner, C.A. / Scott, A.I. / Sacchettini, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 56.3 KB | Display | ![]() |
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PDB format | ![]() | 40.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 506.2 KB | Display | ![]() |
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Full document | ![]() | 527.4 KB | Display | |
Data in XML | ![]() | 9.9 KB | Display | |
Data in CIF | ![]() | 13.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The second part of the functional dimer is generated by the crystallographic two-fold axis: -x, y, -z |
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Components
#1: Protein | Mass: 23217.508 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-COJ / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.18 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 299 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, Magnesium Chloride, Tris-HCl, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 299.0K | ||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 37 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8.5 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Dec 20, 1999 / Details: osmic mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→30 Å / Num. all: 17886 / Num. obs: 5501 / % possible obs: 98.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.4 % / Biso Wilson estimate: 24.3 Å2 / Rmerge(I) obs: 0.106 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 2.6→2.8 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.266 / % possible all: 94.4 |
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 30 Å / Observed criterion σ(I): 2 / Redundancy: 3.4 % / Num. measured all: 17886 |
Reflection shell | *PLUS % possible obs: 98.4 % / Mean I/σ(I) obs: 4.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |