+Open data
-Basic information
Entry | Database: PDB / ID: 3cid | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of BACE Bound to SCH726222 | ||||||
Components | Beta-secretase 1 | ||||||
Keywords | HYDROLASE / BACE1 / Aspartyl protease | ||||||
Function / homology | Function and homology information memapsin 2 / Golgi-associated vesicle lumen / signaling receptor ligand precursor processing / beta-aspartyl-peptidase activity / amyloid precursor protein catabolic process / amyloid-beta formation / membrane protein ectodomain proteolysis / cellular response to manganese ion / prepulse inhibition / amyloid-beta metabolic process ...memapsin 2 / Golgi-associated vesicle lumen / signaling receptor ligand precursor processing / beta-aspartyl-peptidase activity / amyloid precursor protein catabolic process / amyloid-beta formation / membrane protein ectodomain proteolysis / cellular response to manganese ion / prepulse inhibition / amyloid-beta metabolic process / detection of mechanical stimulus involved in sensory perception of pain / cellular response to copper ion / hippocampal mossy fiber to CA3 synapse / presynaptic modulation of chemical synaptic transmission / multivesicular body / response to lead ion / trans-Golgi network / protein processing / recycling endosome / cellular response to amyloid-beta / positive regulation of neuron apoptotic process / synaptic vesicle / late endosome / amyloid-beta binding / peptidase activity / endopeptidase activity / amyloid fibril formation / lysosome / aspartic-type endopeptidase activity / early endosome / endosome membrane / endosome / membrane raft / Amyloid fiber formation / axon / endoplasmic reticulum lumen / dendrite / neuronal cell body / Golgi apparatus / enzyme binding / cell surface / proteolysis / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | ||||||
Authors | Strickland, C. / Cumming, J. | ||||||
Citation | Journal: Bioorg.Med.Chem.Lett. / Year: 2008 Title: Rational design of novel, potent piperazinone and imidazolidinone BACE1 inhibitors Authors: Cumming, J.N. / Le, T.X. / Babu, S. / Carroll, C. / Chen, X. / Favreau, L. / Gaspari, P. / Guo, T. / Hobbs, D.W. / Huang, Y. / Iserloh, U. / Kennedy, M.E. / Kuvelkar, R. / Li, G. / Lowrie, J. ...Authors: Cumming, J.N. / Le, T.X. / Babu, S. / Carroll, C. / Chen, X. / Favreau, L. / Gaspari, P. / Guo, T. / Hobbs, D.W. / Huang, Y. / Iserloh, U. / Kennedy, M.E. / Kuvelkar, R. / Li, G. / Lowrie, J. / McHugh, N.A. / Ozgur, L. / Pan, J. / Parker, E.M. / Saionz, K. / Stamford, A.W. / Strickland, C. / Tadesse, D. / Voigt, J. / Wang, L. / Wu, Y. / Zhang, L. / Zhang, Q. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3cid.cif.gz | 183.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3cid.ent.gz | 150.6 KB | Display | PDB format |
PDBx/mmJSON format | 3cid.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ci/3cid ftp://data.pdbj.org/pub/pdb/validation_reports/ci/3cid | HTTPS FTP |
---|
-Related structure data
Related structure data | 3cibC 3cicC 2qkj C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 43425.961 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BACE1, BACE / Plasmid: pET11a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P56817, memapsin 2 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.46 % |
---|---|
Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 95 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.79→50 Å / Num. all: 95484 / Num. obs: 95380 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 21.3 Å2 / Rmerge(I) obs: 0.051 / Rsym value: 0.051 / Χ2: 1.06 / Net I/σ(I): 25.3 |
Reflection shell | Resolution: 1.79→1.82 Å / Redundancy: 4 % / Rmerge(I) obs: 0.489 / Mean I/σ(I) obs: 2.8 / Num. unique all: 4732 / Rsym value: 0.489 / Χ2: 1.005 / % possible all: 100 |
-Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.8→50 Å / FOM work R set: 0.841 / σ(F): 0 / σ(I): 0
| ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.062 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→50 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
Xplor file |
|