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- EMDB-1843: Structural basis for the subunit assembly of the anaphase promoti... -

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Basic information

Database: EMDB / ID: 1843
TitleStructural basis for the subunit assembly of the anaphase promoting complex
KeywordsAPC/C / anaphase promoting complex
SampleApc4-Apc5 heterodimer
SourceSaccharomyces cerevisiae / yeast / Baker's yeast / サッカロミセス・セレビシエ /
Map dataApc4 and Apc5
Methodsingle particle reconstruction
AuthorsSchreiber A / Stengel F / Zhang Z / Enchev RE / Kong E / Morris EP / Robinson CV / daFonseca PCA / Barford D
CitationNature, 2011, 470, 227-232

Nature, 2011, 470, 227-232 StrPapers
Structural basis for the subunit assembly of the anaphase-promoting complex.
Anne Schreiber / Florian Stengel / Ziguo Zhang / Radoslav I Enchev / Eric H Kong / Edward P Morris / Carol V Robinson / Paula C A da Fonseca / David Barford

DateDeposition: Dec 4, 2010 / Header (metadata) release: Dec 9, 2010 / Map release: Feb 7, 2011 / Last update: Dec 11, 2013

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 52
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by radius
  • Surface level: 52
  • Imaged by UCSF CHIMERA
  • Download
3D viewer

View / / Stereo:
Slabnear <=> far

fix: /
Orientation Rotation
Misc. /
Supplemental images

Downloads & links


Fileemd_1843.map.gz (map file in CCP4 format, 6751 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
120 pix
3.47 Å/pix.
= 416.4 Å
120 pix
3.47 Å/pix.
= 416.4 Å
120 pix
3.47 Å/pix.
= 416.4 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 3.47 Å
Contour Level:52 (by author), 52 (movie #1):
Minimum - Maximum-150.097 - 339.789
Average (Standard dev.)0.606204 (9.63846)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 416.4 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z3.473.473.47
M x/y/z120120120
origin x/y/z0.0000.0000.000
length x/y/z416.400416.400416.400
start NX/NY/NZ-30-24-70
MAP C/R/S123
start NC/NR/NS-60-60-60
D min/max/mean-150.097339.7890.606

Supplemental data

Sample components

Entire Apc4-Apc5 heterodimer

EntireName: Apc4-Apc5 heterodimer / Number of components: 1
MassTheoretical: 160 kDa / Experimental: 155 kDa

Component #1: protein, Apc4_5

ProteinName: Apc4_5 / a.k.a: Apc4_5 / Recombinant expression: Yes
SourceSpecies: Saccharomyces cerevisiae / yeast / Baker's yeast / サッカロミセス・セレビシエ /

Experimental details

Sample preparation

Specimen stateparticle
VitrificationInstrument: NONE / Cryogen name: NONE

Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderHolder: Eucentric / Model: OTHER

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric)

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