[English] 日本語
![](img/lk-miru.gif)
- EMDB-1845: Structural basis for the subunit assembly of the anaphase promoti... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-1845 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structural basis for the subunit assembly of the anaphase promoting complex | |||||||||
![]() | SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23) | |||||||||
![]() |
| |||||||||
![]() | APC/C / anaphase promoting complex | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction | |||||||||
![]() | Schreiber A / Stengel F / Zhang Z / Enchev RE / Kong E / Morris EP / Robinson CV / daFonseca PCA / Barford D | |||||||||
![]() | ![]() Title: Structural basis for the subunit assembly of the anaphase-promoting complex. Authors: Anne Schreiber / Florian Stengel / Ziguo Zhang / Radoslav I Enchev / Eric H Kong / Edward P Morris / Carol V Robinson / Paula C A da Fonseca / David Barford / ![]() Abstract: The anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions. Information on how its 13 constituent proteins ...The anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions. Information on how its 13 constituent proteins are assembled, and how they interact with co-activators, substrates and regulatory proteins is limited. Here, we describe a recombinant expression system that allows the reconstitution of holo APC/C and its sub-complexes that, when combined with electron microscopy, mass spectrometry and docking of crystallographic and homology-derived coordinates, provides a precise definition of the organization and structure of all essential APC/C subunits, resulting in a pseudo-atomic model for 70% of the APC/C. A lattice-like appearance of the APC/C is generated by multiple repeat motifs of most APC/C subunits. Three conserved tetratricopeptide repeat (TPR) subunits (Cdc16, Cdc23 and Cdc27) share related superhelical homo-dimeric architectures that assemble to generate a quasi-symmetrical structure. Our structure explains how this TPR sub-complex, together with additional scaffolding subunits (Apc1, Apc4 and Apc5), coordinate the juxtaposition of the catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-phosphorylation sites, relative to co-activator, regulatory proteins and substrates. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 9.1 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 10.5 KB 10.5 KB | Display Display | ![]() |
Images | ![]() | 123.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 187 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 186.1 KB | Display | |
Data in XML | ![]() | 5.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.47 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23)
Entire | Name: SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23) |
---|---|
Components |
|
-Supramolecule #1000: SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23)
Supramolecule | Name: SC8 (consisting of Apc1, Apc2, Apc4, Apc5, Apc10, Apc11, Mnd2, Cdc23) type: sample / ID: 1000 / Number unique components: 8 |
---|---|
Molecular weight | Experimental: 700 KDa / Theoretical: 690 KDa |
-Macromolecule #1: Apc1
Macromolecule | Name: Apc1 / type: protein_or_peptide / ID: 1 / Name.synonym: Apc1 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #2: Apc2
Macromolecule | Name: Apc2 / type: protein_or_peptide / ID: 2 / Name.synonym: Apc2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #3: Apc4
Macromolecule | Name: Apc4 / type: protein_or_peptide / ID: 3 / Name.synonym: Apc4 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #4: Apc5
Macromolecule | Name: Apc5 / type: protein_or_peptide / ID: 4 / Name.synonym: Apc5 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #5: Apc10
Macromolecule | Name: Apc10 / type: protein_or_peptide / ID: 5 / Name.synonym: Apc10 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #6: Apc11
Macromolecule | Name: Apc11 / type: protein_or_peptide / ID: 6 / Name.synonym: Apc11 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #7: Mnd2
Macromolecule | Name: Mnd2 / type: protein_or_peptide / ID: 7 / Name.synonym: Mnd2 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #8: Cdc23
Macromolecule | Name: Cdc23 / type: protein_or_peptide / ID: 8 / Name.synonym: Cdc23 / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
![]() | single particle reconstruction |
---|---|
Aggregation state | particle |
-
Sample preparation
Vitrification | Cryogen name: NONE / Instrument: OTHER |
---|
-
Electron microscopy
Microscope | FEI TECNAI F20 |
---|---|
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder: Eucentric / Specimen holder model: OTHER |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
-
Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) |
---|