RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E ...RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E / DNA-directed RNA polymerase II subunit RPB1 / DNA-directed RNA polymerase II subunit RPB9 / Transcription elongation factor SPT6 類似検索 - 構成要素
生物種
Homo sapiens (ヒト) / Sus scrofa (ブタ) / synthetic construct (人工物)
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Structure of the transcribing RNA polymerase II-Elongin complex. 著者: Ying Chen / Goran Kokic / Christian Dienemann / Olexandr Dybkov / Henning Urlaub / Patrick Cramer / 要旨: Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to ...Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB-ELOC subunit heterodimer. ELOA contains a 'latch' that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element.
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB3
タンパク質・ペプチド: RNA polymerase II subunit D
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit E
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit F
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB7
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC3
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB9
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC5
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB11-a
タンパク質・ペプチド: RNA polymerase II subunit K
DNA: Non-template DNA
RNA: RNA
DNA: Template DNA
タンパク質・ペプチド: Transcription elongation factor SPT6
リガンド: MAGNESIUM ION
リガンド: ZINC ION
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超分子 #1: The Pol II-SPT6 transcription elongation complex
超分子
名称: The Pol II-SPT6 transcription elongation complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#16 詳細: The complex is a sub-class of a Cry-EM data set of the Pol II-SPT6-Elongin complex.
由来(天然)
生物種: Homo sapiens (ヒト)
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分子 #1: DNA-directed RNA polymerase II subunit RPB1