登録情報 データベース : EMDB / ID : EMD-13831 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of Ty3 retrotransposon targeting a TFIIIB-bound tRNA gene マップデータCryo-EM map of Ty3 intasome targeting RNA Pol III transcribed gene 詳細 試料複合体 : Ty3 retrotransposon engaged with TFIIIB transcription factor and tRNA promoter複合体 : DNADNA : DNA (56-MER)DNA : DNA (31-MER)DNA : DNA (34-MER)DNA : DNA (9-MER)DNA : DNA (19-MER)複合体 : TFIIIB transcription factorタンパク質・ペプチド : Transposon Ty3-G Gag-Pol polyproteinタンパク質・ペプチド : Transcription factor TFIIIB component B''タンパク質・ペプチド : TATA-box-binding proteinタンパク質・ペプチド : Transcription factor IIIB 70 kDa subunit 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
retrotransposon nucleocapsid / RNA polymerase III core binding / retrotransposition / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / DNA-templated transcription open complex formation / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / TFIIIC-class transcription factor complex binding / RNA polymerase III type 3 promoter sequence-specific DNA binding ... retrotransposon nucleocapsid / RNA polymerase III core binding / retrotransposition / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / DNA-templated transcription open complex formation / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / TFIIIC-class transcription factor complex binding / RNA polymerase III type 3 promoter sequence-specific DNA binding / RNA polymerase I general transcription initiation factor binding / RNA polymerase III general transcription initiation factor activity / regulation of transcription by RNA polymerase III / transcription factor TFIIA complex / RNA polymerase I preinitiation complex assembly / transcription preinitiation complex / DNA binding, bending / RNA Polymerase III Transcription Initiation From Type 2 Promoter / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; アスパラギン酸プロテアーゼ / RNA polymerase II transcribes snRNA genes / ribonuclease H / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Polymerase II Pre-transcription Events / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / Estrogen-dependent gene expression / transcription by RNA polymerase III / transcription factor TFIID complex / RNA polymerase II general transcription initiation factor activity / RNA polymerase II core promoter sequence-specific DNA binding / RNA nuclease activity / RNA polymerase II preinitiation complex assembly / TBP-class protein binding / DNA-templated transcription initiation / DNA integration / RNA-directed DNA polymerase / RNA-directed DNA polymerase activity / disordered domain specific binding / RNA-DNA hybrid ribonuclease activity / peptidase activity / DNA-binding transcription factor binding / DNA recombination / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / negative regulation of DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / proteolysis / DNA binding / RNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytoplasm 類似検索 - 分子機能 Peptidase A2B, Ty3 transposon peptidase / Ty3 transposon peptidase / Ty3 transposon capsid-like protein / Ty3 transposon capsid-like protein / Transcription factor TFIIIB component B'', fungi / Brf1, TBP-binding domain / Brf1-like TBP-binding domain / Transcription factor TFIIIB component B'', Myb domain / Myb DNA-binding like / Integrase zinc-binding domain ... Peptidase A2B, Ty3 transposon peptidase / Ty3 transposon peptidase / Ty3 transposon capsid-like protein / Ty3 transposon capsid-like protein / Transcription factor TFIIIB component B'', fungi / Brf1, TBP-binding domain / Brf1-like TBP-binding domain / Transcription factor TFIIIB component B'', Myb domain / Myb DNA-binding like / Integrase zinc-binding domain / Integrase zinc binding domain / Reverse transcriptase/retrotransposon-derived protein, RNase H-like domain / RNase H-like domain found in reverse transcriptase / Transcription factor TFIIB, cyclin-like domain / Transcription factor TFIIB, conserved site / Transcription factor TFIIB repeat / Transcription factor TFIIB repeat signature. / Transcription factor TFIIB / Zinc finger TFIIB-type profile. / Zinc finger, TFIIB-type / TFIIB zinc-binding / TATA-box binding protein, eukaryotic / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / TBP domain superfamily / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Cyclin-like / domain present in cyclins, TFIIB and Retinoblastoma / Cyclin-like superfamily / Homeobox-like domain superfamily / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / zinc finger / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Ribonuclease H superfamily / Aspartic peptidase domain superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily 類似検索 - ドメイン・相同性 TATA-box-binding protein / Transcription factor IIIB 70 kDa subunit / Transcription factor TFIIIB component B'' / Transposon Ty3-G Gag-Pol polyprotein 類似検索 - 構成要素生物種 Saccharomyces cerevisiae S288C (パン酵母)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.98 Å 詳細 データ登録者Abascal-Palacios G / Jochem L / Pla-Prats C / Beuron F / Vannini A 資金援助 英国, 2件 詳細 詳細を隠すOrganization Grant number 国 Cancer Research UK CR-UK C47547/A21536 英国 Wellcome Trust 200818/Z/16/Z 英国
引用ジャーナル : Nat Commun / 年 : 2021タイトル : Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes.著者 : Guillermo Abascal-Palacios / Laura Jochem / Carlos Pla-Prats / Fabienne Beuron / Alessandro Vannini / 要旨 : Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity ... Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window upstream of RNA Polymerase (Pol) III-transcribed genes, representing a paradigm for harmless targeted integration. Here we present the cryo-EM reconstruction at 4.0 Å of an active Ty3 strand transfer complex bound to TFIIIB transcription factor and a tRNA gene. The structure unravels the molecular mechanisms underlying Ty3 targeting specificity at Pol III-transcribed genes and sheds light into the architecture of retrotransposon machinery during integration. Ty3 intasome contacts a region of TBP, a subunit of TFIIIB, which is blocked by NC2 transcription regulator in RNA Pol II-transcribed genes. A newly-identified chromodomain on Ty3 integrase interacts with TFIIIB and the tRNA gene, defining with extreme precision the integration site position. 履歴 登録 2021年11月3日 - ヘッダ(付随情報) 公開 2022年1月26日 - マップ公開 2022年1月26日 - 更新 2022年1月26日 - 現状 2022年1月26日 処理サイト : PDBe / 状態 : 公開
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