- EMDB-13015: Pol II-CSB-CRL4CSA-UVSSA-SPT6-PAF (Structure 4) -
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基本情報
登録情報
データベース: EMDB / ID: EMD-13015
タイトル
Pol II-CSB-CRL4CSA-UVSSA-SPT6-PAF (Structure 4)
マップデータ
The main map.
試料
複合体: Transcribing Pol II bound to CSB, CRL4CSA, UVSSA, PAF and SPT6.
複合体: DNA-directed RNA polymerase with RNA_pol_L_2 and RNA polymerase
タンパク質・ペプチド: x 12種
複合体: Supplementary proteins
タンパク質・ペプチド: x 12種
複合体: NTS, RNA, TS
DNA: x 2種
RNA: x 1種
複合体: E3 ubiquitin-protein ligase RBX1
タンパク質・ペプチド: x 1種
リガンド: x 2種
キーワード
transcription / DNA repair / ubiquitin
機能・相同性
機能・相同性情報
Prolactin receptor signaling / blastocyst growth / Regulation of BACH1 activity / Recognition of DNA damage by PCNA-containing replication complex / negative regulation of double-strand break repair via nonhomologous end joining / DNA Damage Recognition in GG-NER / Formation of TC-NER Pre-Incision Complex / regulation of transcription-coupled nucleotide-excision repair / inner cell mass cell differentiation / Dual Incision in GG-NER ...Prolactin receptor signaling / blastocyst growth / Regulation of BACH1 activity / Recognition of DNA damage by PCNA-containing replication complex / negative regulation of double-strand break repair via nonhomologous end joining / DNA Damage Recognition in GG-NER / Formation of TC-NER Pre-Incision Complex / regulation of transcription-coupled nucleotide-excision repair / inner cell mass cell differentiation / Dual Incision in GG-NER / Dual incision in TC-NER / Ski complex / Formation of Incision Complex in GG-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / positive regulation of mRNA 3'-end processing / nucleotide-excision repair complex / mRNA decay by 3' to 5' exoribonuclease / RNA polymerase II C-terminal domain phosphoserine binding / Regulation of RAS by GAPs / Regulation of RUNX2 expression and activity / Degradation of GLI1 by the proteasome / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Degradation of DVL / Orc1 removal from chromatin / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Cdc73/Paf1 complex / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Hedgehog 'on' state / regulation of isotype switching / negative regulation of granulocyte differentiation / regulation of muscle cell differentiation / Degradation of beta-catenin by the destruction complex / regulation of mRNA export from nucleus / negative regulation of myeloid cell differentiation / endodermal cell fate commitment / eukaryotic initiation factor 4E binding / : / Interleukin-1 signaling / positive regulation of cell cycle G1/S phase transition / blastocyst hatching / anaphase-promoting complex / KEAP1-NFE2L2 pathway / GLI3 is processed to GLI3R by the proteasome / B-WICH complex / trophectodermal cell differentiation / single strand break repair / Neddylation / cullin-RING-type E3 NEDD8 transferase / regulation of mRNA processing / Antigen processing: Ubiquitination & Proteasome degradation / regulation of transcription elongation by RNA polymerase II / nucleosome organization / cullin-RING ubiquitin ligase complex / DNA protection / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / positive regulation by virus of viral protein levels in host cell / regulation of DNA damage checkpoint / ubiquitin-dependent protein catabolic process via the C-end degron rule pathway / Cul7-RING ubiquitin ligase complex / regulation of nucleotide-excision repair / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / spindle assembly involved in female meiosis / epigenetic programming in the zygotic pronuclei / double-strand break repair via classical nonhomologous end joining / response to superoxide / photoreceptor cell maintenance / VCB complex / blastocyst formation / mRNA 3'-end processing / Cul4-RING E3 ubiquitin ligase complex / positive regulation of protein autoubiquitination / UV-damage excision repair / protein neddylation 類似検索 - 分子機能
RNA polymerase II subunit D / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit RPB7 / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E ...RNA polymerase II subunit D / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit RPB7 / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E / DNA-directed RNA polymerase II subunit RPB1 / DNA-directed RNA polymerase II subunit RPB9 / E3 ubiquitin-protein ligase RBX1 / DNA excision repair protein ERCC-6 / DNA excision repair protein ERCC-8 / Cullin-4A / DNA damage-binding protein 1 / UV-stimulated scaffold protein A / Parafibromin / RNA polymerase-associated protein CTR9 homolog / Transcription elongation factor SPT6 / RNA polymerase II-associated factor 1 homolog / RNA polymerase-associated protein LEO1 / Superkiller complex protein 8 類似検索 - 構成要素
生物種
Sus scrofa (ブタ) / Homo sapiens (ヒト) / Mus musculus (ハツカネズミ)
ジャーナル: Nature / 年: 2021 タイトル: Structural basis of human transcription-DNA repair coupling. 著者: Goran Kokic / Felix R Wagner / Aleksandar Chernev / Henning Urlaub / Patrick Cramer / 要旨: Transcription-coupled DNA repair removes bulky DNA lesions from the genome and protects cells against ultraviolet (UV) irradiation. Transcription-coupled DNA repair begins when RNA polymerase II ...Transcription-coupled DNA repair removes bulky DNA lesions from the genome and protects cells against ultraviolet (UV) irradiation. Transcription-coupled DNA repair begins when RNA polymerase II (Pol II) stalls at a DNA lesion and recruits the Cockayne syndrome protein CSB, the E3 ubiquitin ligase, CRL4 and UV-stimulated scaffold protein A (UVSSA). Here we provide five high-resolution structures of Pol II transcription complexes containing human transcription-coupled DNA repair factors and the elongation factors PAF1 complex (PAF) and SPT6. Together with biochemical and published data, the structures provide a model for transcription-repair coupling. Stalling of Pol II at a DNA lesion triggers replacement of the elongation factor DSIF by CSB, which binds to PAF and moves upstream DNA to SPT6. The resulting elongation complex, EC, uses the CSA-stimulated translocase activity of CSB to pull on upstream DNA and push Pol II forward. If the lesion cannot be bypassed, CRL4 spans over the Pol II clamp and ubiquitylates the RPB1 residue K1268, enabling recruitment of TFIIH to UVSSA and DNA repair. Conformational changes in CRL4 lead to ubiquitylation of CSB and to release of transcription-coupled DNA repair factors before transcription may continue over repaired DNA.
凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: FEI VITROBOT MARK IV
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
撮影
フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 40.4 e/Å2
電子線
加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系
照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 倍率(公称値): 81000
実験機器
モデル: Titan Krios / 画像提供: FEI Company
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画像解析
初期モデル
モデルのタイプ: EMDB MAP
最終 再構成
解像度のタイプ: BY AUTHOR / 解像度: 3.0 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION 詳細: Different number of particles was used for different focused refined maps. 使用した粒子像数: 100000