+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-12744 | ||||||||||||||||||
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タイトル | Yeast RNA polymerase II transcription pre-initiation complex with closed distorted promoter DNA | ||||||||||||||||||
マップデータ | |||||||||||||||||||
試料 |
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キーワード | Pre-initiation complex / TRANSCRIPTION | ||||||||||||||||||
機能・相同性 | 機能・相同性情報 regulation of mitotic recombination / RNA polymerase II promoter clearance / RNA polymerase II complex recruiting activity / TFIIA-class transcription factor complex binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / RNA polymerase III transcription regulatory region sequence-specific DNA binding / phosphatidylinositol-5-phosphate binding / positive regulation of mitotic recombination / regulation of transcription by RNA polymerase III ...regulation of mitotic recombination / RNA polymerase II promoter clearance / RNA polymerase II complex recruiting activity / TFIIA-class transcription factor complex binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / RNA polymerase III transcription regulatory region sequence-specific DNA binding / phosphatidylinositol-5-phosphate binding / positive regulation of mitotic recombination / regulation of transcription by RNA polymerase III / nucleotide-excision repair factor 3 complex / RNA polymerase I general transcription initiation factor binding / transcription factor TFIIE complex / DNA translocase activity / nucleotide-excision repair, preincision complex assembly / transcription factor TFIIK complex / transcription open complex formation at RNA polymerase II promoter / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex / transcription factor TFIIF complex / DNA 5'-3' helicase / transcription factor TFIIA complex / phosphatidylinositol-3-phosphate binding / RNA polymerase I preinitiation complex assembly / transcription factor TFIIH holo complex / transcription factor TFIIH core complex / cyclin-dependent protein serine/threonine kinase activator activity / DNA 3'-5' helicase / transcription preinitiation complex / DNA binding, bending / poly(A)+ mRNA export from nucleus / RNA Polymerase I Transcription Initiation / DNA duplex unwinding / Processing of Capped Intron-Containing Pre-mRNA / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA Polymerase III Transcription Initiation From Type 2 Promoter / 3'-5' DNA helicase activity / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / TP53 Regulates Transcription of DNA Repair Genes / termination of RNA polymerase II transcription / transcription factor TFIID complex / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II general transcription initiation factor activity / RNA-templated transcription / RNA Polymerase II Pre-transcription Events / termination of RNA polymerase III transcription / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / termination of RNA polymerase I transcription / RNA Polymerase I Promoter Escape / nucleolar large rRNA transcription by RNA polymerase I / RNA polymerase II complex binding / protein phosphatase activator activity / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription by RNA polymerase I / transcription initiation at RNA polymerase I promoter / Estrogen-dependent gene expression / nuclear-transcribed mRNA catabolic process / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / ATPase activator activity / transcription by RNA polymerase III / RNA polymerase II activity / Dual incision in TC-NER / transcription elongation by RNA polymerase I / positive regulation of transcription initiation by RNA polymerase II / transcription-coupled nucleotide-excision repair / tRNA transcription by RNA polymerase III / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase I activity / RNA polymerase I complex / RNA polymerase III complex / positive regulation of translational initiation / translesion synthesis / ATP-dependent activity, acting on DNA / RNA polymerase II, core complex / RNA polymerase II preinitiation complex assembly / translation initiation factor binding / DNA helicase activity / TBP-class protein binding / isomerase activity / DNA-templated transcription initiation / nucleotide-excision repair / transcription initiation at RNA polymerase II promoter / positive regulation of transcription elongation by RNA polymerase II / transcription elongation by RNA polymerase II / transcription coregulator activity / P-body / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / cytoplasmic stress granule / mRNA processing 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) / synthetic construct (人工物) | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | ||||||||||||||||||
データ登録者 | Schilbach S / Aibara S | ||||||||||||||||||
資金援助 | ドイツ, 5件
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引用 | ジャーナル: Cell / 年: 2021 タイトル: Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening. 著者: Sandra Schilbach / Shintaro Aibara / Christian Dienemann / Frauke Grabbe / Patrick Cramer / 要旨: Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of ...Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30-35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase "clamp head loop" and the TFIIF "charged region" that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems. | ||||||||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
-検証レポート
文書・要旨 | emd_12744_validation.pdf.gz | 814.8 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_12744_full_validation.pdf.gz | 814.4 KB | 表示 | |
XML形式データ | emd_12744_validation.xml.gz | 20.2 KB | 表示 | |
CIF形式データ | emd_12744_validation.cif.gz | 26.2 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-12744 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-12744 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_12744.map.gz / 形式: CCP4 / 大きさ: 209.3 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.05 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
+マスク #1
+追加マップ: Locally filtered map (non-composite) of a CC-dist refinement...
+追加マップ: Half-map 2 of focused refinement encompassing the Ssl1/Tfb4/Tfb1-3-helix-bundle...
+追加マップ: Half-map 1 of focused refinement encompassing the Ssl1/Tfb4/Tfb1-3-helix-bundle...
+追加マップ: Half-map 2 of focused refinement encompassing the Rad3/Ssl1...
+追加マップ: Half-map 1 of focused refinement encompassing the Rad3/Ssl1...
+追加マップ: Half-map 1 of focused refinement encompassing the Ssl2/Tfb2/Tfb5...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Ssl2/Tfb2/Tfb5...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Tfb2...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+追加マップ: Half-map 1 of focused refinement encompassing the Tfb2...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 1 of focused refinement encompassing the Pol...
+追加マップ: Half-map 2 of focused refinement encompassing the Pol...
+ハーフマップ: #2
+ハーフマップ: #1
-試料の構成要素
+全体 : Yeast RNA polymerase II transcription pre-initiation complex with...
+超分子 #1: Yeast RNA polymerase II transcription pre-initiation complex with...
+分子 #1: General transcription and DNA repair factor IIH helicase subunit XPD
+分子 #2: General transcription and DNA repair factor IIH subunit TFB1
+分子 #3: General transcription and DNA repair factor IIH subunit TFB2
+分子 #4: RNA polymerase II transcription factor B subunit 3
+分子 #5: General transcription and DNA repair factor IIH subunit TFB4
+分子 #6: General transcription and DNA repair factor IIH subunit TFB5
+分子 #7: General transcription and DNA repair factor IIH subunit SSL1
+分子 #8: General transcription and DNA repair factor IIH helicase subunit XPB
+分子 #9: DNA-directed RNA polymerase II subunit RPB1
+分子 #10: DNA-directed RNA polymerase II subunit RPB2
+分子 #11: DNA-directed RNA polymerase II subunit RPB3
+分子 #12: DNA-directed RNA polymerase II subunit RPB4
+分子 #13: DNA-directed RNA polymerases I, II, and III subunit RPABC1
+分子 #14: DNA-directed RNA polymerases I, II, and III subunit RPABC2
+分子 #15: DNA-directed RNA polymerase II subunit RPB7
+分子 #16: DNA-directed RNA polymerases I, II, and III subunit RPABC3
+分子 #17: DNA-directed RNA polymerase II subunit RPB9
+分子 #18: DNA-directed RNA polymerases I, II, and III subunit RPABC5
+分子 #19: DNA-directed RNA polymerase II subunit RPB11
+分子 #20: DNA-directed RNA polymerases I, II, and III subunit RPABC4
+分子 #21: Transcription initiation factor IIB
+分子 #23: TATA-box-binding protein
+分子 #24: Transcription initiation factor IIF subunit alpha
+分子 #25: Transcription initiation factor IIF subunit beta
+分子 #27: Transcription initiation factor IIA large subunit
+分子 #28: Transcription initiation factor IIA subunit 2
+分子 #29: Transcription initiation factor IIE subunit alpha
+分子 #30: Transcription initiation factor IIE subunit beta
+分子 #22: Non-template DNA
+分子 #26: Template DNA
+分子 #31: IRON/SULFUR CLUSTER
+分子 #32: ZINC ION
+分子 #33: BERYLLIUM TRIFLUORIDE ION
+分子 #34: MAGNESIUM ION
+分子 #35: ADENOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.6 |
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グリッド | モデル: Quantifoil R3.5/1 / 材質: COPPER / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - 名称: GIF Quantum LS / エネルギーフィルター - スリット幅: 20 eV |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: COUNTING / 実像数: 29670 / 平均露光時間: 9.0 sec. / 平均電子線量: 43.6 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 4.0 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 130000 |
試料ステージ | ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |