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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-11690 | |||||||||
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Title | Human TRPV4 structure in presence of 4a-PDD | |||||||||
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Function / homology | ![]() blood vessel endothelial cell delamination / osmosensor activity / vasopressin secretion / stretch-activated, monoatomic cation-selective, calcium channel activity / positive regulation of striated muscle contraction / calcium ion import into cytosol / positive regulation of macrophage inflammatory protein 1 alpha production / negative regulation of brown fat cell differentiation / positive regulation of microtubule depolymerization / hyperosmotic salinity response ...blood vessel endothelial cell delamination / osmosensor activity / vasopressin secretion / stretch-activated, monoatomic cation-selective, calcium channel activity / positive regulation of striated muscle contraction / calcium ion import into cytosol / positive regulation of macrophage inflammatory protein 1 alpha production / negative regulation of brown fat cell differentiation / positive regulation of microtubule depolymerization / hyperosmotic salinity response / cortical microtubule organization / positive regulation of chemokine (C-X-C motif) ligand 1 production / positive regulation of chemokine (C-C motif) ligand 5 production / cartilage development involved in endochondral bone morphogenesis / cellular hypotonic response / regulation of response to osmotic stress / cellular hypotonic salinity response / multicellular organismal-level water homeostasis / osmosensory signaling pathway / positive regulation of vascular permeability / cellular response to osmotic stress / calcium ion import / cell volume homeostasis / positive regulation of monocyte chemotactic protein-1 production / cell-cell junction assembly / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Botte M / Ulrich AKG / Adaixo R / Gnutt D / Brockmann A / Bucher D / Chami M / Bocquet M / Ebbinghaus-Kintscher U / Puetter V ...Botte M / Ulrich AKG / Adaixo R / Gnutt D / Brockmann A / Bucher D / Chami M / Bocquet M / Ebbinghaus-Kintscher U / Puetter V / Becker A / Egner U / Stahlberg H / Hennig M / Holton SJ | |||||||||
![]() | ![]() Title: Cryo-EM structural studies of the agonist complexed human TRPV4 ion-channel reveals novel structural rearrangements resulting in an open-conformation Authors: Botte M / Ulrich AKG / Adaixo R / Gnutt D / Brockmann A / Bucher D / Chami M / Bocquet M / Ebbinghaus-Kintscher U / Puetter V / Becker A / Egner U / Stahlberg H / Hennig M / Holton SJ | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 481.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.6 KB 11.6 KB | Display Display | ![]() |
Images | ![]() | 44.6 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7aa5MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.639 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Human TRPV4 in complex with 4a-PDD
Entire | Name: Human TRPV4 in complex with 4a-PDD |
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Components |
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-Supramolecule #1: Human TRPV4 in complex with 4a-PDD
Supramolecule | Name: Human TRPV4 in complex with 4a-PDD / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Macromolecule #1: Transient receptor potential cation channel subfamily V member 4,...
Macromolecule | Name: Transient receptor potential cation channel subfamily V member 4,Green fluorescent protein type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 106.24182 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MDYKDDDDKS SMGSRTSLYK KAGSLEVLFQ GPVFNRPILF DIVSRGSTAD LDGLLPFLLT HKKRLTDEEF REPSTGKTCL PKALLNLSN GRNDTIPVLL DIAERTGNMR EFINSPFRDI YYRGQTALHI AIERRCKHYV ELLVAQGADV HAQARGRFFQ P KDEGGYFY ...String: MDYKDDDDKS SMGSRTSLYK KAGSLEVLFQ GPVFNRPILF DIVSRGSTAD LDGLLPFLLT HKKRLTDEEF REPSTGKTCL PKALLNLSN GRNDTIPVLL DIAERTGNMR EFINSPFRDI YYRGQTALHI AIERRCKHYV ELLVAQGADV HAQARGRFFQ P KDEGGYFY FGELPLSLAA CTNQPHIVNY LTENPHKKAD MRRQDSRGNT VLHALVAIAD NTRENTKFVT KMYDLLLLKC AR LFPDSNL EAVLNNDGLS PLMMAAKTGK IGIFQHIIRR EVTDEDTRHL SRKFKDWAYG PVYSSLYDLS SLDTCGEEAS VLE ILVYNS KIENRHEMLA VEPINELLRD KWRKFGAVSF YINVVSYLCA MVIFTLTAYY QPLEGTPPYP YRTTVDYLRL AGEV ITLFT GVLFFFTNIK DLFMKKCPGV NSLFIDGSFQ LLYFIYSVLV IVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRG LKLT GTYSIMIQKI LFKDLFRFLL VYLLFMIGYA SALVSLLNPC ANMKVCNEDQ TDCTVPTYPS CRDSETFSTF LLDLFK LTI GMGDLEMLSS TKYPVVFIIL LVTYIILTFV LLLNMLIALM GETVGQVSKE SKHIWKLQWA TTILDIERSF PVFLRKA FR SGEMVTVGKS SDGTPDRRWC FRVDEVNWSH ENLYFQGAAG SGEFKGEELF TGVVPILVEL DGDVNGHKFS VSGEGEGD A TYGKLTLKFI CTTGKLPVPW PTLVTTLTYG VQCFSRYPDH MKRHDFFKSA MPEGYVQERT ISFKDDGNYK TRAEVKFEG DTLVNRIELK GIDFKEDGNI LGHKLEYNYN SHNVYITADK QKNGIKANFK IRHNIEDGSV QLADHYQQNT PIGDGPVLLP DNHYLSTQS ALSKDPNEKR DHMVLLEFVT AAGITHGMDE LYKASGHHHH HHHHHH |
-Macromolecule #2: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 2 / Number of copies: 1 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 8 |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.0533 kPa |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV / Details: blot for 3 seconds before plunging. |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Initial angle assignment | Type: RANDOM ASSIGNMENT |
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Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.18 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 51215 |