ジャーナル: EMBO J / 年: 2005 タイトル: A quasi-atomic model of human adenovirus type 5 capsid. 著者: Céline M S Fabry / Manuel Rosa-Calatrava / James F Conway / Chloé Zubieta / Stephen Cusack / Rob W H Ruigrok / Guy Schoehn / 要旨: Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent ...Adenoviruses infect a wide range of vertebrates including humans. Their icosahedral capsids are composed of three major proteins: the trimeric hexon forms the facets and the penton, a noncovalent complex of the pentameric penton base and trimeric fibre proteins, is located at the 12 capsid vertices. Several proteins (IIIa, VI, VIII and IX) stabilise the capsid. We have obtained a 10 A resolution map of the human adenovirus 5 by image analysis from cryo-electron micrographs (cryoEMs). This map, in combination with the X-ray structures of the penton base and hexon, was used to build a quasi-atomic model of the arrangement of the two major capsid components and to analyse the hexon-hexon and hexon-penton interactions. The secondary proteins, notably VIII, were located by comparing cryoEM maps of native and pIX deletion mutant virions. Minor proteins IX and IIIa are located on the outside of the capsid, whereas protein VIII is organised with a T=2 lattice on the inner face of the capsid. The capsid organisation is compared with the known X-ray structure of bacteriophage PRD1.
想定した対称性 - 点群: I (正20面体型対称) / アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 10.3 Å / 解像度の算出法: FSC 0.33 CUT-OFF / ソフトウェア - 名称: PFT EM3DR / 詳細: Final maps were calculated from 4100 single images / 使用した粒子像数: 4100
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原子モデル構築 1
ソフトウェア
名称: URO SITUS
詳細
Protocol: Rigid Body. Penton bases were fitted using SITUS Hexons from the asymetric unit were fitted togheter using URO
精密化
空間: RECIPROCAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: R-factor
得られたモデル
PDB-4v4u: The quasi-atomic model of Human Adenovirus type 5 capsid