EMDB-0099: tRNA translocation by the eukaryotic 80S ribosome and the impact ...

Basic information

• Entry: Database: EMDB / ID: EMD-0099
• Title: tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)

Sample

• Complex: TI-POST-2
  • Protein or peptide: x 79 types
  • RNA: x 7 types
• Ligand: x 3 types

• Biological species: Oryctolagus cuniculus (rabbit)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Flis J / Holm M / Rundlet EJ / Loerke J / Hilal T / Dabrowski M / Buerger J / Mielke T / Blanchard SC / Spahn CMT / Budkevich TV

Funding support

Germany, United States, 3 items

Organization	Grant number	Country
German Research Foundation	DFG SFB 740	Germany
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM079238	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	T32 GM115327-Tan	United States

• Citation: Journal: Cell Rep / Year: 2018; Title: tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.; Authors: Julia Flis / Mikael Holm / Emily J Rundlet / Justus Loerke / Tarek Hilal / Marylena Dabrowski / Jörg Bürger / Thorsten Mielke / Scott C Blanchard / Christian M T Spahn / Tatyana V Budkevich / ; Abstract: Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site.

History

Deposition	Jul 3, 2018	-
Header (metadata) release	Oct 24, 2018	-
Map release	Dec 5, 2018	-
Update	Mar 30, 2022	-
Current status	Mar 30, 2022	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_0099.map.gz / Format: CCP4 / Size: 253.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.975 Å

Density

Contour Level	By AUTHOR: 2.5 / Movie #1: 2.5
Minimum - Maximum	-3.981005 - 12.587396
Average (Standard dev.)	0.3450845 (±0.67152554)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 405 405 405 Spacing 405 405 405
Cell	A=B=C: 394.875 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	0.975	0.975	0.975
M x/y/z	405	405	405
origin x/y/z	0.000	0.000	0.000
length x/y/z	394.875	394.875	394.875
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	450	450	450
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	405	405	405
D min/max/mean	-3.981	12.587	0.345

Supplemental data

Sample components

Entire : TI-POST-2

• Entire: Name: TI-POST-2

Components

• Complex: TI-POST-2
  • Protein or peptide: ribosomal protein uL2
  • Protein or peptide: ribosomal protein uS2
  • Protein or peptide: ribosomal protein uL3
  • Protein or peptide: ribosomal protein eS1
  • Protein or peptide: ribosomal protein uL4
  • Protein or peptide: ribosomal protein eS28
  • RNA: 28S ribosomal RNA
  • RNA: ap/P-site tRNA
  • RNA: mRNA
  • RNA: pe/E-site-tRNA
  • RNA: 18S ribosomal RNA
  • Protein or peptide: ribosomal protein uS3
  • Protein or peptide: ribosomal protein uS7
  • Protein or peptide: ribosomal protein eS10
  • Protein or peptide: ribosomal protein eS12
  • Protein or peptide: ribosomal protein uS19
  • Protein or peptide: ribosomal protein uS9
  • Protein or peptide: ribosomal protein eS17
  • Protein or peptide: ribosomal protein uS13
  • Protein or peptide: ribosomal protein eS19
  • Protein or peptide: ribosomal protein uS10
  • Protein or peptide: ribosomal protein uS10
  • Protein or peptide: ribosomal protein eS28
  • Protein or peptide: ribosomal protein uS14
  • Protein or peptide: ribosomal protein eS31
  • Protein or peptide: ribosomal protein RACK 1
  • Protein or peptide: ribosomal protein eS4
  • Protein or peptide: ribosomal protein eS6
  • Protein or peptide: ribosomal protein eS7
  • Protein or peptide: ribosomal protein eS8
  • Protein or peptide: ribosomal protein uS4
  • Protein or peptide: ribosomal protein uS17
  • Protein or peptide: ribosomal protein uS15
  • Protein or peptide: ribosomal protein uS11
  • Protein or peptide: ribosomal protein eS21
  • Protein or peptide: ribosomal protein uS8
  • Protein or peptide: ribosomal protein uS12
  • Protein or peptide: ribosomal protein eS24
  • Protein or peptide: ribosomal protein eS26
  • Protein or peptide: ribosomal protein eS27
  • Protein or peptide: ribosomal protein eS30
  • RNA: 5.8S ribosomal RNA
  • RNA: 5S ribosomal RNA
  • Protein or peptide: ribosomal protein uL18
  • Protein or peptide: ribosomal protein eL6
  • Protein or peptide: ribosomal protein uL30
  • Protein or peptide: ribosomal protein eL8
  • Protein or peptide: ribosomal protein uL6
  • Protein or peptide: ribosomal protein uL16
  • Protein or peptide: ribosomal protein uL5
  • Protein or peptide: ribosomal protein eL13
  • Protein or peptide: ribosomal protein eL14
  • Protein or peptide: ribosomal protein eL15
  • Protein or peptide: ribosomal protein uL13
  • Protein or peptide: ribosomal protein uL22
  • Protein or peptide: ribosomal protein eL18
  • Protein or peptide: ribosomal protein eL19
  • Protein or peptide: ribosomal protein eL20
  • Protein or peptide: ribosomal protein eL21
  • Protein or peptide: ribosomal protein eL22
  • Protein or peptide: ribosomal protein uL14
  • Protein or peptide: ribosomal protein eL24
  • Protein or peptide: ribosomal protein uL23
  • Protein or peptide: ribosomal protein uL24
  • Protein or peptide: ribosomal protein eL27
  • Protein or peptide: ribosomal protein uL15
  • Protein or peptide: ribosomal protein eL29
  • Protein or peptide: ribosomal protein eL30
  • Protein or peptide: ribosomal protein eL31
  • Protein or peptide: ribosomal protein eL32
  • Protein or peptide: ribosomal protein eL33
  • Protein or peptide: ribosomal protein eL34
  • Protein or peptide: ribosomal protein uL29
  • Protein or peptide: ribosomal protein eL36
  • Protein or peptide: ribosomal protein eL37
  • Protein or peptide: ribosomal protein eL38
  • Protein or peptide: ribosomal protein eL39
  • Protein or peptide: ribosomal protein eL40
  • Protein or peptide: ribosomal protein eL41
  • Protein or peptide: ribosomal protein eL42
  • Protein or peptide: ribosomal protein eL43
  • Protein or peptide: ribosomal protein eL28
  • Protein or peptide: ribosomal protein uL1
  • Protein or peptide: ribosomal protein uL11
  • Protein or peptide: ribosomal protein uL10
  • Protein or peptide: eukaryotic elongation factor 2 (eEF2)
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

Supramolecule #1: TI-POST-2

• Supramolecule: Name: TI-POST-2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#86
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 4.2 MDa

Macromolecule #1: ribosomal protein uL2

• Macromolecule: Name: ribosomal protein uL2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 27.456158 KDa

Sequence

String:

GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGRLRG TKTVQ

Macromolecule #2: ribosomal protein uS2

• Macromolecule: Name: ribosomal protein uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.24574 KDa

Sequence

String:

LDVLQMKEED VLKFLAAGTH LGGTNLDFQM EQYIYKRKSD GIYIINLKRT WEKLLLAARA IVAIENPADV SVISSRNTGQ RAVLKFAAA TGATPIAGRF TPGTFTNQIQ AAFREPRLLV VTDPRADHQP LTEASYVNLP TIALCNTDSP LRYVDIAIPC N NKGAHSVG LMWWMLAREV LRMRGTISRE HPWEVMPDLY FYRDPEEIEK EEQAAAE

Macromolecule #3: ribosomal protein uL3

• Macromolecule: Name: ribosomal protein uL3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 45.222949 KDa

Sequence

String:

SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDE DGKKQLEKDF SSMKKYCQVI R VIAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVNQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LP RKTHRGL RKVACIGAWH PARVAFSVAR AGQKGYHHRT EINKKIYKIG QGYLIKDGKL IKNNASTDYD LSDKSINPLG GFV HYGEVT NDFVMLKGCV VGTKKRVLTL RKSLLVQTKR RALEKIDLKF IDTTSKFGHG RFQTMEEKKA FMGPLKKD

Macromolecule #4: ribosomal protein eS1

• Macromolecule: Name: ribosomal protein eS1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.660012 KDa

Sequence

String:

VDPFSKKDWY DVKAPAMFNI RNIGKTLVTR TQGTKIASDG LKGRVFEVSL ADLQNDEVAF RKFKLITEDV QGKNCLTNFH GMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND L KEVVNKLI PDSIGKDIEK ACQSIYPLHD VFVRKVKMLK KPKFELGKLM ELHG

Macromolecule #5: ribosomal protein uL4

• Macromolecule: Name: ribosomal protein uL4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 41.171547 KDa

Sequence

String:

CARPLISVYS EKGESSGKNV TLPAVFKAPI RPDIVNFVHT NLRKNNRQPY AVSELAGHQT SAESWGTGRA VARIPRVRGG GTHRSGQGA FGNMCRGGRM FAPTKTWRRW HRRVNTTQKR YAICSALAAS ALPALVMSKG HRIEEVPELP LVVEDKVEGY K KTKEAVLL LKKLKAWNDI KKVYASQRMR AGKGKMRNRR RIQRRGPCII YNEDNGIIKA FRNIPGITLL NVSKLNILKL AP GGHVGRF CIWTESAFRK LDELYGTWRK AASLKSNYNL PMHKMINTDL SRILKSPEIQ RALRAPRKKI HRRVLKKNPL KNL RIMLKL NPYAKTMRRN TILRQARNHK LRVDKAAAAA AALQAKS

Macromolecule #6: ribosomal protein eS28

• Macromolecule: Name: ribosomal protein eS28 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.574971 KDa

Sequence

String:

DKEWMPVTKL GRLVKDMKIK SLEEIYLFSL PIKESEIIDF FLGASLKDEV LKIMPVQKQT RAGQRTRFKA FVAIGDYNGH VGLGVKCSK EVATAIRGAI ILAKLSIVPV RRGYWGNKIG KPHTVPCKVT GRCGSVLVRL IPAPRGTGIV SAPVPKKLLM M AGIDDCYT SARGCTATLG NFAKATFDAI SKTYSYLTPD LWKETVFTKS PYQEFTDHLV KTHT

Macromolecule #12: ribosomal protein uS3

• Macromolecule: Name: ribosomal protein uS3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.35759 KDa

Sequence

String:

AVQISKKRKF VADGIFKAEL NEFLTRELAE DGYSGVEVRV TPTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS G DPVNYYVD TAVRHVLLRQ GVLGIKVKIM LPWDPTGKIG PKKPLPDHVS IVEPKDEILP TT

Macromolecule #13: ribosomal protein uS7

• Macromolecule: Name: ribosomal protein uS7 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.424836 KDa

Sequence

String:

PDIKLFGKWS TDDVQINDIS LQDYIAVKEK YAKYLPHSAG RYAAKRFRKA QCPIVERLTN SMMMHGRNNG KKLMTVRIVK HAFEIIHLL TGENPLQVLV NAIINSGPRE DSTRIGRAGT VRRQAVDVSP LRRVNQAIWL LCTGAREAAF RNIKTIAECL A DELINAAK GSSNSYAIKK KDELERVAKS NR

Macromolecule #14: ribosomal protein eS10

• Macromolecule: Name: ribosomal protein eS10 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.773953 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSR

Macromolecule #15: ribosomal protein eS12

• Macromolecule: Name: ribosomal protein eS12 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.393658 KDa

Sequence

String:

VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWVG LCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK C

Macromolecule #16: ribosomal protein uS19

• Macromolecule: Name: ribosomal protein uS19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.273879 KDa

Sequence

String:

FRKFTYRGVD LDQLLDMSYE QLMQLYSARQ RRRLNRGLRR KQHSLLKRLR KAKKEAPPME KPEVVKTHLR DMIILPEMVG SMVGVYNGK TFNQVEIKPE MIGHYLGEFS ITYKPVKHGR P

Macromolecule #17: ribosomal protein uS9

• Macromolecule: Name: ribosomal protein uS9 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.76548 KDa

Sequence

String:

QSVQVFGRKK TATAVAHCKR GNGLIKVNGR PLEMIEPRTL QYKLLEPVLL LGKERFAGVD IRVRVKGGGH VAQIYAIRQS ISKALVAYY QKYVDEASKK EIKDILIQYD RTLLVADPRR CESKKFGGPG ARARYQKSYR

Macromolecule #18: ribosomal protein eS17

• Macromolecule: Name: ribosomal protein eS17 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.447793 KDa

Sequence

String:

GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGM

Macromolecule #19: ribosomal protein uS13

• Macromolecule: Name: ribosomal protein uS13 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.383064 KDa

Sequence

String:

VIPEKFQHIL RVLNTNIDGR RKIAFAITAI KGVGRRYAHV VLRKADIDLT KRAGELTEDE VERVITIMQN PRQYKIPDWF LNRQKDVKD GKYSQVLANG LDNKLREDLE RLKKIRAHRG LRHFWGLRVR GQHTKTTGRR

Macromolecule #20: ribosomal protein eS19

• Macromolecule: Name: ribosomal protein eS19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.822219 KDa

Sequence

String:

PGVTVKDVNQ QEFVRALAAF LKKSGKLKVP EWVDTVKLAK HKELAPYDEN WFYTRAASTA RHLYLRGGAG VGSMTKIYGG RQRNGVMPS HFSRGSKSVA RRVLQALEGL KMVEKDQDGG RKLTPQGQRD LDRIAGQVAA ANKK

Macromolecule #21: ribosomal protein uS10

• Macromolecule: Name: ribosomal protein uS10 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.029072 KDa

Sequence

String:

IRITLTSRNV KSLEKVCADL IRGAKEKNLK VKGPVRMPTK TLRITTRKTP CGEGSKTWDR FQMRIHKRLI DLHSPSEIVK QITSISIEP GVEVEVTI

Macromolecule #22: ribosomal protein uS10

• Macromolecule: Name: ribosomal protein uS10 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.801748 KDa

Sequence

String:

GKAKKKKWSK GKVRDKLNNL VLFDKATYDK LCKEVPNYKL ITPAVVSERL KIRGSLARAA LQELLSKGLI KLVSKHRAQV IYTRNT

Macromolecule #23: ribosomal protein eS28

• Macromolecule: Name: ribosomal protein eS28 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.007069 KDa

Sequence

String:

QPIKLARVTK VLGRTGSQGQ CTQVRVEFMD DTSRSIIRNV KGPVREGDVL TLLESEREAR RL

Macromolecule #24: ribosomal protein uS14

• Macromolecule: Name: ribosomal protein uS14 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.12121 KDa

Sequence

String:

QLYWSHPRKF GQGSRSCRVC SNRHGLIRKY GLNMCRQCFR QYAKDIGFIK L

Macromolecule #25: ribosomal protein eS31

• Macromolecule: Name: ribosomal protein eS31 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.645277 KDa

Sequence

String:

KRKKKSYTTP KKNKHKRKKV KLAVLKYYKV DENGKISRLR RECPSDECGA GVFMASHFDR HYCGKCCLTY CFN

Macromolecule #26: ribosomal protein RACK 1

• Macromolecule: Name: ribosomal protein RACK 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.726164 KDa

Sequence

String:

TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTIG

Macromolecule #27: ribosomal protein eS4

• Macromolecule: Name: ribosomal protein eS4 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.893043 KDa

Sequence

String:

ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIVFLRNR LKYALTGDEV KKICMQRFIK IDGKVRVDVT YPAGFMDVI SIEKTGEHFR LVYDTKGRFA VHRITVEEAK YKLCKVRKIT VGVKGIPHLV THDARTIRYP DPVIKVNDTV Q IDLGTGKI INFIKFDTGN LCMVIGGANL GRVGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG NGNKPWISLP RG KGIRLTV AEERDKRLAT

Macromolecule #28: ribosomal protein eS6

• Macromolecule: Name: ribosomal protein eS6 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 26.913867 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRR

Macromolecule #29: ribosomal protein eS7

• Macromolecule: Name: ribosomal protein eS7 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.990465 KDa

Sequence

String:

NGEKPDEFES GISQALLELE MNSDLKAQLR ELNITAAKEI EVGGGRKAII IFVPVPQLKS FQKIQVRLVR ELEKKFSGKH VVFIAQRRI LPKPTRKSRT KNKQKRPRSR TLTAVHDAIL EDLVFPSEIV GKRIRVKLDG SRLIKVHLDK AQQNNVEHKV E TFSGVYKK LTGKDVNFEF PEFQL

Macromolecule #30: ribosomal protein eS8

• Macromolecule: Name: ribosomal protein eS8 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.132191 KDa

Sequence

String:

GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKGK

Macromolecule #31: ribosomal protein uS4

• Macromolecule: Name: ribosomal protein uS4 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.222221 KDa

Sequence

String:

PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK K

Macromolecule #32: ribosomal protein uS17

• Macromolecule: Name: ribosomal protein uS17 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.910176 KDa

Sequence

String:

TERAYQKQPT IFQNKKRVLL GETGKEKLPR YYKNIGLGFK TPKEAIEGTY IDKKCPFTGN VSIRGRILSG VVTKMKMQRT IVIRRDYLH YIRKYNRFEK RHKNMSVHLS PCFRDVQIGD IVTVGECRPL SKTVRFNVLK VTKAAGTKKQ FQKF

Macromolecule #33: ribosomal protein uS15

• Macromolecule: Name: ribosomal protein uS15 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.057113 KDa

Sequence

String:

GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV

Macromolecule #34: ribosomal protein uS11

• Macromolecule: Name: ribosomal protein uS11 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.544659 KDa

Sequence

String:

SLGPQVAEGE NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQRC KELGITALHI KLRATGGNR TKTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRRL

Macromolecule #35: ribosomal protein eS21

• Macromolecule: Name: ribosomal protein eS21 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.863111 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS K

Macromolecule #36: ribosomal protein uS8

• Macromolecule: Name: ribosomal protein uS8 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.734357 KDa

Sequence

String:

VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

Macromolecule #37: ribosomal protein uS12

• Macromolecule: Name: ribosomal protein uS12 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.626392 KDa

Sequence

String:

GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

Macromolecule #38: ribosomal protein eS24

• Macromolecule: Name: ribosomal protein eS24 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.489176 KDa

Sequence

String:

TVTIRTRKFM TNRLLQRKQM VIDVLHPGKA TVPKTEIREK LAKMYKTTPD VIFVFGFRTH FGGGKTTGFG MIYDSLDYAK KNEPKHRLA RHGLYEKKKT SRKQRKERKN RMKKVRGTAK ANVGAG

Macromolecule #39: ribosomal protein eS26

• Macromolecule: Name: ribosomal protein eS26 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.087117 KDa

Sequence

String:

TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPP

Macromolecule #40: ribosomal protein eS27

• Macromolecule: Name: ribosomal protein eS27 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.00057 KDa

Sequence

String:

AKDLLHPSPE EEKRKHKKKR LVQSPNSYFM DVKCPGCYKI TTVFSHAQTV VLCVGCSTVL CQPTGGKARL TEGCSFRRKQ

Macromolecule #41: ribosomal protein eS30

• Macromolecule: Name: ribosomal protein eS30 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.239447 KDa

Sequence

String:

HGSLARAGKV RGQTPKVAKQ EKKKKKTGRA KRRMQYNRRF VNVVPTFGKK KGPNA

Macromolecule #44: ribosomal protein uL18

• Macromolecule: Name: ribosomal protein uL18 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.091371 KDa

Sequence

String:

VKVVKNKAYF KRYQVKFRRR REGKTDYYAR KRLVIQDKNK YNTPKYRMIV RVTNRDIICQ IAYARIEGDM IVCAAYAHEL PKYGVKVGL TNYAAAYCTG LLLARRLLNR FGMDKIYEGQ VEVTGDEYNV ESIDGQPGAF TCYLDAGLAR TTTGNKVFGA L KGAVDGGL SIPHSTKRFP GYDSESKEFN AEVHRKHIMG QNVADYMRYL MEEDEDAYKK QFSQYIKNSV TPDMMEEMYK KA HAAIREN PVYEKKPKKE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES

Macromolecule #45: ribosomal protein eL6

• Macromolecule: Name: ribosomal protein eL6 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.262428 KDa

Sequence

String:

PVGGDKNGGT RVVKLRKMPR YYPTEDVPRK LLSHGKKPFS QHVRKLRASI TPGTILIILT GRHRGKRVVF LKQLASGLLL VTGPLVLNR VPLRRTHQKF VIATSTKIDI SNVKIPKHLT DAYFKKKKLR KPRHQEGEIF DTEKEKYEIT EQRKIDQKAV D SQILPKIK AIPQLQGYLR SVFALTNGIY PHKLVF

Macromolecule #46: ribosomal protein uL30

• Macromolecule: Name: ribosomal protein uL30 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 27.733146 KDa

Sequence

String:

PETLKKKRRN FAELKIKRLR KKFAQKMLRK ARRKLIYEKA KHYHKEYRQM YRTEIRMARM ARKAGNFYVP AEPKLAFVIR IRGINGVSP KVRKVLQLLR LRQIFNGTFV KLNKASINML RIVEPYIAWG YPNLKSVNEL IYKRGYGKIN KKRIALTDNA L IARSLGKY GIICMEDLIH EIYTVGKRFK EANNFLWPFK LSSPRGGMKK KTTHFVEGGD AGNREDQINR LIRRMN

Macromolecule #47: ribosomal protein eL8

• Macromolecule: Name: ribosomal protein eL8 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 26.724568 KDa

Sequence

String:

NPLFEKRPKN FGIGQDIQPK RDLTRFVKWP RYIRLQRQRA ILYKRLKVPP AINQFTQALD RQTATQLLKL AHKYRPETKQ EKKQRLLAR AEKKAAGKGD VPTKRPPVLR AGVNTVTTLV ENKKAQLVVI AHDVDPIELV VFLPALCRKM GVPYCIIKGK A RLGRLVHR KTCTTVAFTQ VNSEDKGALA KLVEAIRTNY NDRYDEIRRH WGGNVLGPKS VARIAKLEKA KAKELA

Macromolecule #48: ribosomal protein uL6

• Macromolecule: Name: ribosomal protein uL6 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.770355 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QAD

Macromolecule #49: ribosomal protein uL16

• Macromolecule: Name: ribosomal protein uL16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.150484 KDa

Sequence

String:

RRPARCYRYC KNKPYPKSRF CRGVPDAKIR IFDLGRKKAK VDEFPLCGHM VSDEYEQLSS EALEAARICA NKYMVKSCGK DGFHIRVRL HPFHVIRINK MLSADRLQTG MRGAFGKPQG TVARVHIGQV IMSIRTKLQN KEHVIEALRR AKFKFPGRQK I HISKKWGF TKFNADEFED MVAEKRLIPD GCGVKYIPNR GPLDKWRALH

Macromolecule #50: ribosomal protein uL5

• Macromolecule: Name: ribosomal protein uL5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 19.270318 KDa

Sequence

String:

ENPMRELRIR KLCLNICVGE SGDRLTRAAK VLEQLTGQTP VFSKARYTVR SFGIRRNEKI AVHCTVRGAK AEEILEKGLK VREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ Q KYDGIILP G

Macromolecule #51: ribosomal protein eL13

• Macromolecule: Name: ribosomal protein eL13 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.574697 KDa

Sequence

String:

APSRNGMVLK PHFHKDWQRR VATWFNQPAR KIRRRKARQA KARRIAPRPA SGPIRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLILFPRK PSAPKKGDSS AEELKLATQL TGPVMPVRNV Y KKEKARVI TEEEKNFKAF ASLRMARANA RLFGIRAKRA KEAAEQD

Macromolecule #52: ribosomal protein eL14

• Macromolecule: Name: ribosomal protein eL14 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.220309 KDa

Sequence

String:

VFRRFVEVGR VAYVSFGPHA GKLVAIVDVI DQNRALVDGP CTQVRRQAMP FKCMQLTDFI LKFPHSAHQK YVRQAWQKAD INTKWAATR WAKKIEARER KAKMTDFDRF KVMKAKKMRN RIIKNEVKKL QKAALLKASP

Macromolecule #53: ribosomal protein eL15

• Macromolecule: Name: ribosomal protein eL15 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.076088 KDa

Sequence

String:

GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR KSRGLGKGHK FHHTIGGSRR AAWRRRNTLQ LHRYR

Macromolecule #54: ribosomal protein uL13

• Macromolecule: Name: ribosomal protein uL13 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.820395 KDa

Sequence

String:

QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK QLMRLRKQAE KNVEKKIDKY TEVLKTH

Macromolecule #55: ribosomal protein uL22

• Macromolecule: Name: ribosomal protein uL22 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.757627 KDa

Sequence

String:

VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLQKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

Macromolecule #56: ribosomal protein eL18

• Macromolecule: Name: ribosomal protein eL18 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.556482 KDa

Sequence

String:

GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGRENKTAV VVGTITDDV RVQEVPKLKV CALRVTSRAR SRILRAGGKI LTFDQLALDS PKGCGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN

Macromolecule #57: ribosomal protein eL19

• Macromolecule: Name: ribosomal protein eL19 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.742146 KDa

Sequence

String:

SMLRLQKRLA SSVLRCGKKK VWLDPNETNE IANANSRQQI RKLIKDGLII RKPVTVHSRA RCRKNTLARR KGRHMGIGKR KGTANARMP EKVTWMRRMR ILRRLLRRYR ESKKIDRHMY HSLYLKVKGN VFKNKRILME HIHKLKADKA RKKLLADQAE A RRSKTKEA RKRREERLQA KKE

Macromolecule #58: ribosomal protein eL20

• Macromolecule: Name: ribosomal protein eL20 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.66134 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTF

Macromolecule #59: ribosomal protein eL21

• Macromolecule: Name: ribosomal protein eL21 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.276518 KDa

Sequence

String:

TNTKGKRRGT RYMFSRPFRK HGVVPLATYM RIYKKGDIVD IKGMGTVQKG MPHKCYHGKT GRVYNVTQHA VGIVVNKQVK GKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR QPAPPREAHF VRTNGKEPEL LEPIPYEF

Macromolecule #60: ribosomal protein eL22

• Macromolecule: Name: ribosomal protein eL22 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.481248 KDa

Sequence

String:

VLKFTLDCTH PVEDGIMDAA NFEQFLQERI KVNGKAGNLG GGVVTIERSK SKITVTSEVP FSKRYLKYLT KKYLKKNNLR DWLRVVANS KESYELRYFQ

Macromolecule #61: ribosomal protein uL14

• Macromolecule: Name: ribosomal protein uL14 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.828295 KDa

Sequence

String:

AKFRISLGLP VGAVINCADN TGAKNLYIIS VKGIKGRLNR LPAAGVGDMV MATVKKGKPE LRKKVHPAVV IRQRKSYRRK DGVFLYFED NAGVIVNNKG EMKGSAITGP VAKECADLWP RIASNAGSIA

Macromolecule #62: ribosomal protein eL24

• Macromolecule: Name: ribosomal protein eL24 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.087417 KDa

Sequence

String:

KVELCSFSGY KIYPGHGRRY ARTDGKVFQF LNAKCESAFL SKRNPRQINW TVLYRRKHKK GQSEEIQKKR TRRAVKFQRA ITGASLADI MAKRNQKPEV RKAQREQAIR AAKEAKKAKQ AS

Macromolecule #63: ribosomal protein uL23

• Macromolecule: Name: ribosomal protein uL23 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.614023 KDa

Sequence

String:

KIRTSPTFRR PKTLRLRRQP KYPRKSAPRR NKLDHYAIIK FPLTTESAMK KIEDNNTLVF IVDVKANKHQ IKQAVKKLYD IDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGI

Macromolecule #64: ribosomal protein uL24

• Macromolecule: Name: ribosomal protein uL24 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.161899 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQ

Macromolecule #65: ribosomal protein eL27

• Macromolecule: Name: ribosomal protein eL27 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.647584 KDa

Sequence

String:

KFMKPGKVVL VLAGRYSGRK AVIVKNIDDG TSDRPYSHAL VAGIDRYPRK VTAAMGKKKI AKRSKIKSFV KVYNYNHLMP TRYSVDIPL DKTVVNKDVF RDPALKRKAR REAKVKFEER YKTGKNKWFF QKLRF

Macromolecule #66: ribosomal protein uL15

• Macromolecule: Name: ribosomal protein uL15 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.47334 KDa

Sequence

String:

PSRLRKTRKL RGHVSHGHGR IGKHRKHPGG RGNAGGLHHH RINFDKYHPG YFGKVGMKHY HLKRNQSFCP TVNLDKLWTL VSEQTRVNA AKNKTGAAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKSV GGACVLVA

Macromolecule #67: ribosomal protein eL29

• Macromolecule: Name: ribosomal protein eL29 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.996388 KDa

Sequence

String:

AKSKNHTTHN QSRKWHRNGI KKPRSQRYES LKGVDPKFLR NMRFAKKHNK KGLKKMQANN AKAMSARA

Macromolecule #68: ribosomal protein eL30

• Macromolecule: Name: ribosomal protein eL30 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.500556 KDa

Sequence

String:

TKKSLESINS RLQLVMKSGK YVLGYKQTLK MIRQGKAKLV ILANNCPALR KSEIEYYAML AKTGVHHYSG NNIELGTACG KYYRVCTLA IIDPGDSDII RSMP

Macromolecule #69: ribosomal protein eL31

• Macromolecule: Name: ribosomal protein eL31 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.506581 KDa

Sequence

String:

NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLV TYVPVTTFKN LQTVNVD

Macromolecule #70: ribosomal protein eL32

• Macromolecule: Name: ribosomal protein eL32 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.179215 KDa

Sequence

String:

AALRPLVKPK IVKKRTKKFI RHQSDRYVKI KRNWRKPRGI DNRVRRRFKG QILMPNIGYG SNKKTKHMLP SGFRKFLVHN VKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARLR

Macromolecule #71: ribosomal protein eL33

• Macromolecule: Name: ribosomal protein eL33 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.433548 KDa

Sequence

String:

SGRLWSKAIF AGYKRGLRNQ REHTALLKIE GVYARDETEF YLGKRCAYVY KAKNNTVTPG GKPNKTRVIW GKVTRAHGNS GMVRAKFRS NLPAKAIGHR IRVMLYPSRI

Macromolecule #72: ribosomal protein eL34

• Macromolecule: Name: ribosomal protein eL34 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.994621 KDa

Sequence

String:

VQRLTYRRRL SYNTASNKTR LSRTPGNRIV YLYTKKVGKA PKSACGVCPG RLRGVRAVRP KVLMRLSKTK KHVSRAYGGS MCAKCVRDR IKRAFLIEEQ KIVVKVLKAQ AQSQK

Macromolecule #73: ribosomal protein uL29

• Macromolecule: Name: ribosomal protein uL29 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.462429 KDa

Sequence

String:

AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLNKHEE NLKTKKQQRK ERLYPLRKYA VKA

Macromolecule #74: ribosomal protein eL36

• Macromolecule: Name: ribosomal protein eL36 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.373708 KDa

Sequence

String:

YPMAVGLNKG HKVTKNVSKP RHSRRRGRLT KHTKFVRDMI REVCGFAPYE RRAMELLKVS KDKRALKFIK KRVGTHIRAK RKREELSNV LAAMRKAA

Macromolecule #75: ribosomal protein eL37

• Macromolecule: Name: ribosomal protein eL37 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.864596 KDa

Sequence

String:

TKGTSSFGKR RNKTHTLCRR CGSKAYHLQK STCGKCGYPA KRKRKYNWSA KAKRRNTTGT GRMRHLKIVY RRFRHGFREG TTPK

Macromolecule #76: ribosomal protein eL38

• Macromolecule: Name: ribosomal protein eL38 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.107752 KDa

Sequence

String:

PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKELK

Macromolecule #77: ribosomal protein eL39

• Macromolecule: Name: ribosomal protein eL39 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.295562 KDa

Sequence

String:

SSHKTFRIKR FLAKKQKQNR PIPQWIRMKT GNKIRYNSKR RHWRRTKLGL

Macromolecule #78: ribosomal protein eL40

• Macromolecule: Name: ribosomal protein eL40 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 5.957237 KDa

Sequence

String:

IEPSLRQLAQ KYNCDKMICR KCYARLHPRA VNCRKKKCGH TNNLRPKKKV

Macromolecule #79: ribosomal protein eL41

• Macromolecule: Name: ribosomal protein eL41 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

Macromolecule #80: ribosomal protein eL42

• Macromolecule: Name: ribosomal protein eL42 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.345776 KDa

Sequence

String:

VNVPKTRRTF CKKCGKHQPH KVTQYKKGKD SLYAQGKRRY DRKQSGYGGQ TKPIFRKKAK TTKKIVLRLE CVEPNCRSKR MLAIKRCKH FELGGDKKRK GQVIQF

Macromolecule #81: ribosomal protein eL43

• Macromolecule: Name: ribosomal protein eL43 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 10.168153 KDa

Sequence

String:

AKRTKKVGIV GKYGTRYGAS LRKMVKKIEI SQHAKYTCSF CGKTKMKRRA VGIWHCGSCM KTVAGGAWTY NTTSAVTVKS AIRRLKELK DQ

Macromolecule #82: ribosomal protein eL28

• Macromolecule: Name: ribosomal protein eL28 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.991366 KDa

Sequence

String:

SAHLQWMVVR NCSSFLIKRN KQTYSTEPNN LKARNSFRYN GLIHRKTVGV EPAADGKGVV VVIKRRSGQR KPATSYVRTT INKNARATL SSIRHMIRKN KYRPDLRMAA IRRASAILRS QKP

Macromolecule #83: ribosomal protein uL1

• Macromolecule: Name: ribosomal protein uL1 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.879422 KDa

Sequence

String:

MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

Macromolecule #84: ribosomal protein uL11

• Macromolecule: Name: ribosomal protein uL11 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.290794 KDa

Sequence

String:

VYLRCTGGEV GATSALAPKI GPLGLSPKKV GDDIAKATGD WKGLRITVKL TIQNRQAQIE VVPSASALII KALKEPPRDR KKQKNIKHS GNITFDEIVN IARQMRHRSL ARELSGTIKE ILGTAQSVGC NVDGRHPHDI IDDINSGAVE CP

Macromolecule #85: ribosomal protein uL10

• Macromolecule: Name: ribosomal protein uL10 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.21784 KDa

Sequence

String:

DRATWKSNYF LKIIQLLDDY PKCFIVGADN VGSKQMQQIR MSLRGKAVVL MGKNTMMRKA IRGHLENNPA LEKLLPHIRG NVGFVFTKE DLTEIRDMLR ANKVPAAARA GAIAPCEVTV PAQNTGLGPE KTSFFQALGI TTKISRGTIE ILSDVQLIKT G DKVGASEA TLLNMLNISP FSFGLVIQQV FDNGSIYNPE VLDI

Macromolecule #86: eukaryotic elongation factor 2 (eEF2)

• Macromolecule: Name: eukaryotic elongation factor 2 (eEF2) / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 95.056734 KDa

Sequence

String:

TVDQIRAIMD KKANIRNMSV IAHVDHGKST LTDSLVCKAG IIASARAGET RFTDTRKDEQ ERCITIKSTA ISLFYELSEN DLNFIKQSK DGAGFLINLI DSPGHVDFSS EVTAALRVTD GALVVVDCVS GVCVQTETVL RQAIAERIKP VLMMNKMDRA L LELQLEPE ELYQTFQRIV ENVNVIISTY GEGESGPMGN IMIDPVLGTV GFGSGLHGWA FTLKQFAEMY VAKFAAKGEG QL GPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLD SEDKDK EGKPLLKAVM RRWLPAGDAL LQMITIHLPS PVTAQKYRCE LLYEGPPDDE AAMGIKSCDP KGPLMMYISK MVPT SDKGR FYAFGRVFSG LVSTGLKVRI MGPNYTPGKK EDLYLKPIQR TILMMGRYVE PIEDVPCGNI VGLVGVDQFL VKTGT ITTF EHAHNMRVMK FSVSPVVRVA VEAKNPADLP KLVEGLKRLA KSDPMVQCII EESGEHIIAG AGELHLEICL KDLEED HAC IPIKKSDPVV SYRETVSEES NVLCLSKSPN KHNRLYMKAR PFPDGLAEDI DKGEVSARQE LKQRARYLAE KYEWDVA EA RKIWCFGPDG TGPNILTDIT KGVQYLNEIK DSVVAGFQWA TKEGALCEEN MRGVRFDVHD VTLHADAI(DDE)R GGGQ IIPTA RRCLYASVLT AQPRLMEPIY LVEIQCPEQV VGGIYGVLNR KRGHVFEESQ VAGTPMFVVK AYLPVNESFG FTADL RSNT GGQAFPQCVF DHWQILPGDP FDNSSRPSQV VAETRKRKGL KEIPALDNFL DKL

Macromolecule #7: 28S ribosomal RNA

• Macromolecule: Name: 28S ribosomal RNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 1.170164875 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGGGCG CGGGACAUGU GGCGUACGGA A GACCCGCU CCCCGGCGCC GCUCGUGGGG GGCCCAAGUC CUUCUGAUCG AGGCCCAGCC CGUGGACGGU GUGAGGCCGG UA GCGGCCC CCGGCGCGCG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG GGUCC GGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCC CCCGGGGUCG GCGGGGGACC GUCCCCCGGA CCGGCGACCG GCCGCC GCC GGGCGCAUUU CCAGGCGGUG CGCCGCGACC GGCUCCGGGA CGGCUGGGAA GGCCCGGCGG GGAAGGUGGC UCGGGGC CC CCGAGUGUUA CAGCCCCCCC GGCAGCAGCA CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUCGCC GCGCUCUC C CCCCUGGGGG GGCCGGGCCA CCCCUCCCAC GGCGCGACCG CUCUCCCAGG GGGCGGGGCG GACUGUCCCC AGUGCGCCC CGGGCGGGUC GCGCCGUCGG GCCCGGGGGG CCACGCGCGC GUCCCGGGAC GGCGGAGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUA CCCACCCGAC CCGUCUUGAA ACACGGACCA AGGAGUCUAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA G CCGCCGUG GCGCAAUGAA GGUGAAGGCC GGCGCGCUCG CCGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GG GGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACCCG AAAGAUGGUG AAC UAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAAA UCGGUCGUCC GACC UGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGGC GCUCU CGCC CACGCAGUUU UAUCCGGUAA AGCGAAUGAU UAGAGGUCUU GGGGCCGAAA CGAUCUCAAC CUAUUCUCAA ACUUUA AAU GGGUAAGAAG CCCGGCUCGC UGGCGUGGAG CCGGGGUGGA AUGCGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAA CU GGCGCUGCGG GAUGAACCGA ACGCCGGGUU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUU G AUAUAGACAG CAGGACGGUG GCCAUGGAAG UCGGAAUCCG CUAAGGAGUG UGUAACAACU CACCUGCCGA AUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCGUCGG GCCCAUACCC GGCCGUCGCC GGCCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAG CCUUGAAGCC UAGGGCGCGG GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU U CAAACGAG AACUUUGAAG GCCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AG AUGGGCG AGCGCCGUUC CGAAGGGACG GGCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCC CCGAAU CCGGAGUGGC GGAGAUGGGC GCCGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAG CCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUU GGAA AGCGUCGCGG UUCCGGCGGC GUCCGGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGC GCC GGGCCGUACC CAUAUCCGCA GCAGGUCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGU CG GCAAGCCGGA UCCGUAACUU CGGGAUAAGG AUUGGCUCUA AGGGCUGGCA GCCGACUUAG AACUGGUGCG GACCAGGG G AAUCCGACUG UUUAAUUAAA ACAAAGCAUC GCGAAGGCCC GCGGCGGGUG UUGACGCGAU GUGAUUUCUG CCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAAUGAA GCGCGGGUAA ACGGCGGGAG UAACUAUGAC UCUCUUAAGG UAGCCAAAUG CCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUGAAC GAGAUUCCCA CUGUCCCUAC CUACUAUCCA GCGAAACCAC A GCCAAGGG AACGGGCUUG GCGGAAUCAG CGGGGAAAGA AGACCCUGUU GAGCUUGACU CUAGUCUGGC ACGGUGAAGA GA CAUGAGA GGUGUAGAAU AAGUGGGAGG CCCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGU GAGGCG GGGGGGCGAG CCCGAGGGGC UCUCGCUUCU GGCGCCAAGC GCCCGCCCGG CCGGGCGCGA CCCGCUCCGG GGAC AGUGC CAGGUGGGGA GUUUGACUGG GGCGGUACAC CUGUCAAACG GUAACGCAGG UGUCCUAAGG CGAGCUCAGG GAGGA CAGA AACCUCCCGU GGAGCAGAAG GGCAAAAGCU CGCUUGAUCU UGAUUUUCAG UACGAAUACA GACCGUGAAA GCGGGG CCU CACGAUCCUU CUGACCUUUU GGGUUUUAAG CAGGAGGUGU CAGAAAAGUU ACCACAGGGA UAACUGGCUU GUGGCGG CC AAGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUUGUGA AGCAGAAUUC GCCAAGCG U UGGAUUGUUC ACCCACUAAU AGGGAACGUG AGCUGGGUUU AGACCGUCGU GAGACAGGUU AGUUUUACCC UACUGAUGA UGUGUUGUUG CCAUGGUAAU CCUGCUCAGU ACGAGAGGAA CCGCAGGUUC AGACAUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGG GCGAAGCUAC CAUCUGUGGG AUUAUGACUG AACGCCUCUA AGUCAGAAUC CCGCCCAGGC GGAACGAUAC G GCAGCGCC GCGGAGCCUC GGUUGGCCUC GGAUAGCCGG UCCGGGGUCC GGUGCGGAGU GCCCUUCGUC CUGGGAAACG GG GCGCGGC CGGAAAGGCG GCCGCCCCCU CGCCCGUCAC GCACCGCACG UUCGUGGGGA ACCUGGCGCU AAACCAUUCG UAG ACGACC UGCUUCUGGG UCGGGGUUUC GUACGUAGCA GAGCAGCUCC CUCGCUGCGA UCUAUUGAAA GUCAGCCCUC GACA CAAGG GUUUGUC

Macromolecule #8: ap/P-site tRNA

• Macromolecule: Name: ap/P-site tRNA / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.437535 KDa

Sequence

String:

GUUUCCGUAG UGUAGUGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCC CGGUUCGAAA CCGGGCGGAA ACACCA

Macromolecule #9: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 3.492122 KDa

Sequence

String:

UUCGUUAAAG A

Macromolecule #10: pe/E-site-tRNA

• Macromolecule: Name: pe/E-site-tRNA / type: rna / ID: 10 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.533586 KDa

Sequence

String:

GCGGACUUAG CUCAGUUGGG AGAGCGCCAG ACUGAAGAUC UGGAGGUCCU GUGUUCGAUC CACAGAGUUC GCACCA

Macromolecule #11: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 551.108188 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGACU UGGAUAACUG UGGUAAUUCU AGAGCUAAUA C AUGCCGAC GGGCGCUGAC CCCCUUCGCG GGGGGGAUGC GUGCAUUUAU CAGAUCAAAA CCAACCCGGG CUUUGGUGAC UC UAGAUAA CCUCGGGCCG AUCGCACGCC CCCCGUGGCG GCGACGACCC AUUCGAACGU CUGCCCUAUC AACUUUCGAU GGU AGUCGC CGUGCCUACC AUGGUGACCA CGGGUGACGG GGAAUCAGGG UUCGAUUCCG GAGAGGGAGC CUGAGAAACG GCUA CCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCACUCCCGA CCCGGGGAGG UAGUGACGAA AAAUAACAAU ACAGG ACUC UUUCGAGGCC CUGUAAUUGG AAUGAGUCCA CUUUAAAUCC UUUAACGAGG AUCCAUUGGA GGGCAAGUCU GGUGCC AGC AGCCGCGGUA AUUCCAGCUC CAAUAGCGUA UAUUAAAGUU GCUGCAGUUA AAAAGCUCGU AGUUGGAUCU UGGGAGC GG CCCCUUGCCU CUCGCCCGAA GCGUUUACUU UGAAAAAAUU AGAGUGUUCA AAGCAGGCCC GAGCCGCCUG GAUACCGC A GCUAGGAAUA AUGGAAUAGG ACCGCGGUUC UAUUUUGUUG GUUUUCGGAA CUGAGGCCAU GAUUAAGAGG GACGGCCGG GGGCAUUCGU AUUGCGCCGC UAGAGGUGAA AUUCUUGGAC CGGCGCAAGA CGGACCAGAG CGAAAGCAUU UGCCAAGAAU GUUUUCAUU AAUCAAGAAC GAAAGUCGGA GGUUCGAAGA CGAUCAGAUA CCGUCGUAGU UCCGACCAUA AACGAUGCCG A CCGGCGAU GCGGCGGCGU UAUUCCCAUG ACCCGCCGGG CAGCUUCCGG GAAACCAAAG UCUUUGGGUU CCGGGGGGAG UA UGGUUGC AAAGCUGAAA CUUAAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACA CGGGAA ACCUCACCCG GCCCGGACAC GGACAGGAUU GACAGAUUGA UAGCUCUUUC UCGAUUCCGU GGGUGGUGGU GCAU GGCCG UUCUUAGUUG GUGGAGCGAU UUGUCUGGUU AAUUCCGAUA ACGAACGAGA CUCUGGCAUG CUAACUAGUU ACGCG ACCC CCGAGCGGUA ACUUCUUAGA GGGACAAGUG GCGUUCAGCC ACCCGAGAUU GAGCAAUAAC AGGUCUGUGA UGCCCU UAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUAC CCUACGCCGG CAGGCGCGGG UAACCCG UU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAUUC CCCAUGAACG AGGAAUUCCC AGUAAGUGCG GGUCAUAA G CUUGCGUUGA UUAAGUCCCU GCCCUUUGUA CACACCGCCC GUCGCUACUA CCGAUUGGAU GGUUUAGUGA GGCCCUCGG AUCGGCCCCG CCGUGGCGGA GCGCUGAGAA GACGGUCGAA CUUGACUAUC UAGAGGAAGU AAAAGUCGUA ACAAGGUUUC CGUAGGUGA ACCUGCGGAA GGAUCAUUA

Macromolecule #42: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 42 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 50.449812 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

Macromolecule #43: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 43 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 38.38575 KDa

Sequence

String:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU

Macromolecule #87: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 87 / Number of copies: 327 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #88: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 88 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #89: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

• Macromolecule: Name: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 89 / Number of copies: 1 / Formula: GNP
• Molecular weight: Theoretical: 522.196 Da

Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI POLARA 300
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 30.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Tecnai Polara / Image courtesy: FEI Company

Image processing

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 37941
• Initial angle assignment: Type: PROJECTION MATCHING
• Final angle assignment: Type: PROJECTION MATCHING

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-6gz4:
tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)