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- PDB-6gz5: tRNA translocation by the eukaryotic 80S ribosome and the impact ... -
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Basic information
Entry | Database: PDB / ID: 6gz5 | ||||||||||||||||||||||||
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Title | tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-3 (TI-POST-3) | ||||||||||||||||||||||||
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![]() | Flis, J. / Holm, M. / Rundlet, E.J. / Loerke, J. / Hilal, T. / Dabrowski, M. / Buerger, J. / Mielke, T. / Blanchard, S.C. / Spahn, C.M.T. / Budkevich, T.V. | ||||||||||||||||||||||||
![]() | Journal: Cell Rep / Year: 2018 Title: tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis. ![]() Abstract: Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes ...Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site. | ||||||||||||||||||||||||
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Date | Deposition: Jul 3, 2018 / Release: Dec 5, 2018
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Structure visualization
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-Related structure data
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Assembly
Deposited unit | ![]()
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1 |
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Components
-RNA chain , 7 types, 7 molecules A2BvBxBwB1A3A4
#1: RNA chain | ![]() Mass: 1170164.875 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
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#2: RNA chain | Mass: 24437.535 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
#3: RNA chain | ![]() Mass: 3492.122 Da / Num. of mol.: 1 Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() |
#4: RNA chain | Mass: 24533.586 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
#5: RNA chain | ![]() Mass: 551108.188 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
#39: RNA chain | ![]() Mass: 50449.812 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
#40: RNA chain | ![]() Mass: 38385.750 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
+Ribosomal protein ... , 78 types, 78 molecules BDBFBKBMBPBQBRBSBTBUBZBcBdBfBgBABBBCBEBGBHBIBJBLBNBOBVBWBXBY...
-Protein/peptide , 1 types, 1 molecules Ct
#86: Protein/peptide | Mass: 95056.734 Da / Num. of mol.: 1 / Source: (natural) ![]() ![]() ![]() |
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-Non-polymers , 3 types, 333 molecules 




#87: Chemical | ChemComp-MG / #88: Chemical | ChemComp-ZN / #89: Chemical | ChemComp-GNP / | |
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-Experimental details
-Experiment
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EM experiment | Aggregation state: PARTICLE / Reconstruction method: ![]() |
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Sample preparation
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