DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 ...DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / General transcription and DNA repair factor IIH subunit TFB1 / DNA-directed RNA polymerase II subunit RPB7 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / RNA polymerase II transcription factor B subunit 3 / General transcription and DNA repair factor IIH subunit SSL1 / General transcription and DNA repair factor IIH subunit TFB4 / General transcription and DNA repair factor IIH subunit TFB5 類似検索 - 構成要素
ジャーナル: Mol Cell / 年: 2019 タイトル: Promoter Distortion and Opening in the RNA Polymerase II Cleft. 著者: Christian Dienemann / Björn Schwalb / Sandra Schilbach / Patrick Cramer / 要旨: Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron ...Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron microscopic (cryo-EM) structure of a yeast PIC that contains underwound, distorted promoter DNA in the closed Pol II cleft. The DNA duplex axis is offset at the upstream edge of the initially melted DNA region (IMR) where DNA opening begins. Unstable IMRs are found in a subset of yeast promoters that we show can still initiate transcription after depletion of the transcription factor (TF) IIH (TFIIH) translocase Ssl2 (XPB in human) from the nucleus in vivo. PIC-induced DNA distortions may thus prime the IMR for melting and may explain how unstable IMRs that are predicted in promoters of Pol I and Pol III can open spontaneously. These results suggest that DNA distortion in the polymerase cleft is a general mechanism that contributes to promoter opening.
タンパク質・ペプチド: General transcription and DNA repair factor IIH helicase subunit XPD,General transcription and DNA repair factor IIH helicase subunit XPD,DNA repair helicase RAD3,DNA repair helicase RAD3,General transcription and DNA repair factor IIH helicase subunit XPD
タンパク質・ペプチド: General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1
タンパク質・ペプチド: General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2
タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 3
タンパク質・ペプチド: General transcription and DNA repair factor IIH subunit TFB4,General transcription and DNA repair factor IIH subunit TFB4,RNA polymerase II transcription factor B subunit 4,General transcription and DNA repair factor IIH subunit TFB4,General transcription and DNA repair factor IIH subunit TFB4
タンパク質・ペプチド: General transcription and DNA repair factor IIH subunit TFB5
タンパク質・ペプチド: General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1
タンパク質・ペプチド: General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB
複合体: Polymerase
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB1
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB2
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB3
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB4
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC1
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB7
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC3
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB9
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC5
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB11
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC4
複合体: Nucleic acid
DNA: non-template DNA (HIS4)
DNA: Template DNA (HIS4)
複合体: transcription factors
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC2
UniProtKB: General transcription and DNA repair factor IIH helicase subunit XPD/RAD3, General transcription and DNA repair factor IIH helicase subunit XPD/RAD3
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分子 #2: General transcription and DNA repair factor IIH subunit TFB1,Gene...
分子
名称: General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General ...名称: General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,RNA polymerase II transcription factor B subunit 1,General transcription and DNA repair factor IIH subunit TFB1,General transcription and DNA repair factor IIH subunit TFB1 タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
UniProtKB: General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1
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分子 #3: General transcription and DNA repair factor IIH subunit TFB2,Gene...
分子
名称: General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General ...名称: General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2,General transcription and DNA repair factor IIH subunit TFB2 タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
UniProtKB: General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, ...UniProtKB: General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2
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分子 #4: RNA polymerase II transcription factor B subunit 3
UniProtKB: RNA polymerase II transcription factor B subunit 3
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分子 #5: General transcription and DNA repair factor IIH subunit TFB4,Gene...
分子
名称: General transcription and DNA repair factor IIH subunit TFB4,General transcription and DNA repair factor IIH subunit TFB4,RNA polymerase II transcription factor B subunit 4,General ...名称: General transcription and DNA repair factor IIH subunit TFB4,General transcription and DNA repair factor IIH subunit TFB4,RNA polymerase II transcription factor B subunit 4,General transcription and DNA repair factor IIH subunit TFB4,General transcription and DNA repair factor IIH subunit TFB4 タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
UniProtKB: General transcription and DNA repair factor IIH subunit TFB5
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分子 #7: General transcription and DNA repair factor IIH subunit SSL1,Gene...
分子
名称: General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General ...名称: General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1,General transcription and DNA repair factor IIH subunit SSL1 タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
UniProtKB: General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, ...UniProtKB: General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1
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分子 #8: General transcription and DNA repair factor IIH helicase subunit ...
分子
名称: General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and ...名称: General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,DNA repair helicase RAD25,General transcription and DNA repair factor IIH helicase subunit XPB,General transcription and DNA repair factor IIH helicase subunit XPB タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2
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分子 #9: DNA-directed RNA polymerase II subunit RPB1